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Mouse zygote-specific proteasome assembly chaperone important for maternal-to-zygotic transition
- Source :
- Biology Open, Biology Open, Vol 2, Iss 2, Pp 170-182 (2012)
- Publication Year :
- 2012
- Publisher :
- The Company of Biologists, 2012.
-
Abstract
- Summary During the maternal-to-zygotic transition (MZT), maternal proteins in oocytes are degraded by the ubiquitin–proteasome system (UPS), and new proteins are synthesized from the zygotic genome. However, the specific mechanisms underlying the UPS at the MZT are not well understood. We identified a molecule named zygote-specific proteasome assembly chaperone (ZPAC) that is specifically expressed in mouse gonads, and expression of ZPAC was transiently increased at the mouse MZT. ZPAC formed a complex with Ump1 and associated with precursor forms of 20S proteasomes. Transcription of ZPAC genes was also under the control of an autoregulatory feedback mechanism for the compensation of reduced proteasome activity similar to Ump1 and 20S proteasome subunit gene expression. Knockdown of ZPAC in early embryos caused a significant reduction of proteasome activity and decrease in Ump1 and mature proteasomes, leading to accumulation of proteins that need to be degraded at the MZT and early developmental arrest. Therefore, a unique proteasome assembly pathway mediated by ZPAC is important for progression of the mouse MZT.
- Subjects :
- Genetics
Gene knockdown
Zygote
Maternal-to-zygotic transition
QH301-705.5
Science
Biology
General Biochemistry, Genetics and Molecular Biology
Cell biology
Proteasome
Transcription (biology)
Chaperone (protein)
Proteasome assembly
Ubiquitin–proteasome system
biology.protein
Maternal to zygotic transition
Biology (General)
General Agricultural and Biological Sciences
ZPAC
Gene
Research Article
Subjects
Details
- ISSN :
- 20466390
- Volume :
- 2
- Database :
- OpenAIRE
- Journal :
- Biology Open
- Accession number :
- edsair.doi.dedup.....50f74c2b4f965379688b20ac234983e8