Author: "Chang A" / Topic: biology - Searchworks@Jio Institute Digital Library Search Results

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1. Professional development. Summer institute to improve university science teaching.

Author: Pfund C, Miller S, Brenner K, Bruns P, Chang A, Ebert-May D, Fagen AP, Gentile J, Gossens S, Khan IM, Labov JB, Pribbenow CM, Susman M, Tong L, Wright R, Yuan RT, Wood WB, and Handelsman J
Subjects: Education, Continuing, Faculty, Universities, Academies and Institutes, Biology education, Teaching
Published: 2009
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2. Data integration gets 'Sloppy'.

Author: Almeida JS, Chen C, Gorlitsky R, Stanislaus R, Aires-de-Sousa M, Eleutério P, Carriço J, Maretzek A, Bohn A, Chang A, Zhang F, Mitra R, Mills GB, Wang X, and Deus HF
Subjects: Systems Integration, Biology methods, Database Management Systems, Databases, Factual, Documentation methods, Information Dissemination methods, Information Storage and Retrieval methods
Published: 2006
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3. A Cross-Disciplinary Study on the Forms and Functions of Citations in the Discussion Sections of Master's Theses in Taiwan

Author: Baring, June April M. and Chang, Peichin
Abstract: Effective citation contributes to the success in master's (MA) thesis writing. The current study investigates cross-disciplinary citation practice in EFL Master's theses. First, the corpus was compiled by collecting 20 Applied Linguistics (AL) and 20 Biology (BIO) MA theses Discussion sections. The forms and rhetorical functions of citations were then identified and quantified. The results show that the writers from the two disciplines have different citation practices. In terms of forms, the AL discipline writers utilized both integral and non-integral forms almost equally. The BIO discipline writers, on the other hand, deployed significantly more non-integral citations. In terms of rhetorical functions, citations were used by both groups to achieve a variety of rhetorical functions. The AL discipline writers utilized citations mostly for Comparison and Application to provide explanation and justification. By contrast, the BIO discipline writers took a more descriptive approach by using more Attribution. Overall, the results suggest that while the AL writers seemed to align their citation forms and functions, the BIO writers adopted a more simplified or descriptive approach when citing. The study offers evidence in the need to guide EFL writers of different disciplines to becoming more strategic in their citation practice.
Published: 2023

4. Argument Visualization with DMaps: Cases from Postsecondary Learning

Author: John Nesbit, Qing Liu, Joan Sharp, Diana Cukierman, Holly Hendrigan, Daniel Chang, Bahareh Shahabi, Qingyan Deng, and Azar Pakdaman Savoji
Abstract: The Dialectical Map (DMap) is an open-source, web-based argument visualization tool developed and used at a Canadian University to scaffold argument construction. To illustrate the ways that argument mapping can be used in undergraduate courses, this article presents five cases selected from courses in biology, psychology, computing science, and English as a foreign language offered at three post-secondary institutions. Each case explains how argument mapping with DMaps (DMapping) was implemented and assessed in a course. Students responded to a questionnaire that gathered their attitudes toward DMapping as a learning activity. In each course, students were also interviewed about their DMapping experiences. The interview and questionnaire data indicated that students believed DMapping was an effective way to meet the knowledge objectives of their course and to learn about argumentation. The authors explain how DMap assignments added value to their courses by helping students think critically about course topics while developing their argumentation ability and information literacy. Finally, we summarize the lessons learned across the cases and discuss ways of maximizing the benefits of argument mapping activities for postsecondary learning.
Published: 2024

5. BDNF Val66Met Polymorphism Moderates Negative Symptom Expression of Bully Victimization through Resilience in Taiwanese Youth

Author: Chih-Ting Lee, Chung-Ying Lin, Carol Strong, Yun-Hsuan Chang, Yi-Ching Lin, Yi-Ping Hsieh, Yu-Fang Lin, and Meng-Che Tsai
Abstract: Bully victimization is known to cause adverse psychological outcomes; however, resilience may mitigate the more adverse effects. Little is known regarding the role played by BDNF Val66Met polymorphism in youth resilience against psychological harm caused by bully victimization. In this cross-sectional study, a community sample of 598 participants (M[subscript age] = 20.1 ± 1.4 years, 48.8% males) completed the questionnaire on bully victimization, resilience, and psychological symptoms. Salivary genomic DNA was genotyped for the BDNF Val66Met polymorphism. A path analysis was used to test the mediating role of resilience in the association between bully victimization and psychological symptoms. Furthermore, the BDNF genotype was added to the model to explore its moderating effects on the mediating role of resilience in the path with 5000 bootstrapped samplings using SPSS PROCESS Macro. Results revealed a significant indirect effect via resilience that accounted for 17.2% of the association between bully victimization and psychological symptoms. While the Val66Met polymorphism interacted with bully victimization to predict resilience scores, bully victimization was more strongly associated with poor resilience (F = 4.59, p = 0.03) in subjects with the Met/Met genotype ([beta] = -3.22, p < 0.001), as compared to participants with other genotypes ([beta] = -1.33, p = 0.051). Findings suggest a gene-environment interaction effect on psychological resilience in bully-victimized youth.
Published: 2024
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6. Place-Based Education through Immersive Virtual Experiences -- Preparing Biology Students for the Field

Author: Jan Oliver Wallgrün, Jack Shen-Kuen Chang, Jiayan Zhao, Peter Trenham, Pejman Sajjadi, Mark Simpson, and Alexander Klippel
Abstract: Virtual field trips are transforming education in biology and other place-based disciplines -- and not just since COVID-19 has imposed social distancing constraints. Efficient methods for content creation, combined with affordable immersive technology, provide the opportunity to integrate immersive experiences into ever-larger classes. However, many aspects of how immersive experiences can enrich and improve learning in place-based education are still not well understood. We present an empirical study, conducted in a large undergraduate ecology class, comparing students, that experienced an immersive virtual version of a field trip to a biological preserve as preparation for a later actual field trip to the site, with a control group that visited the real site but without having had the virtual experience. Within the virtual experience group, we also varied the quality of the headset. The VR field trip was well received for raising spatial awareness and also motivation and interest. Students ranked it higher than any traditional means with regard to achieving a high level of preparedness for the actual field trip. In contrast, no statistically significant evidence was found for differences in perceived learning outcome and enjoyment of the actual field trip.
Published: 2024
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7. Measuring Attitude toward Chemistry, Biology, and Math at a Hispanic-Serving Institution

Author: Chang, Jordan and Menke, Erik Jason
Abstract: This work describes the evaluation of the Attitude toward the Subject of Chemistry Inventory (ASCI), as well as two modifications (one for measuring attitude toward math and one for measuring attitude toward biology), for college students at a Hispanic Serving Institution. Instrument reliability was tested via multiple administrations of the instruments, and confirmatory factor analysis supported a two-factor structure similar to an existing model of a revised version of the ASCI for all three instruments. The similar factor structure of the three instruments, coupled with interviews with students, provide validity evidence for the instruments and support an interpretation that one of the subscales aligns with a cognitive aspect of attitude while the other subscale aligns with an affective aspect. The results of these instruments indicate that students have a more positive attitude toward biology than either chemistry or math, and more positive affective attitude than cognitive attitude for all three subjects, although student attitudes show little change with respect to biology, chemistry, or math during a typical semester. However, major perturbations, such as switching to remote instruction midsemester, can lead to small but significant increases and decreases in attitude.
Published: 2022
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8. Metacognitive Inquiry Activities for Instructing the Central Dogma Concept: 'Button Code' and 'Beaded Bracelet Making'

Author: Chang, Pei-Shan, Lee, Sheng-Han, and Wen, Meichun Lydia
Abstract: The central dogma of biology is difficult to learn because its microscopic processes cannot be visualized. This study aimed to devise two inquiry activities: 'Button Code' and 'Beaded Bracelet Making,' involving the concepts of DNA replication and protein synthesis based on the Metacognitive Learning Cycle (MLC) for students, and to explore the effectiveness of concept learning of the central dogma, how students' metacognition may be expressed, and students' perceptions of their inquiry performance. We developed a 'Concept journal' including metacognitive scaffolds, and employed the 'Central Dogma Achievement Test' as a tool for the above purpose. A total of 18 junior high school students participated in this inquiry course instructed by two of the authors. The results showed that students' achievement performance was significantly improved on the whole, the students' metacognition was expressed during the process of inquiry with scaffolding, and most students gave positive responses about their learning performance. According to the results, this inquiry course could develop students' comprehension of the central dogma concept, and give students opportunities to practice metacognition that might lead to effective learning in inquiry activities. The implications and expandability of this course are discussed.
Published: 2020
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9. A Senior Teacher's Implementation of Technology Integration

Author: Tsai, Hsien-Chang
Abstract: This study investigated whether a senior teacher with many years of teaching experience, despite lacking adequate technology skills or contending with other barriers, can sufficiently implement technology integration in the classroom. The research was conducted between October 2013 and January 2014 and was focused on a junior high school biology teacher with 17 years of instructional experience. A qualitative method involving semistructured interviews and classroom observation of 4 conducted lessons was used to explore the teacher's implementation of technology integration. The analytical results showed that the teacher's perspectives on technology integration ranged from "technology as a tool for teaching" to "technology as a learning activator." Moreover, external factors such as poor technology management affected the teacher's implementation of technology integration. In addition, the teacher developed a technological-pedagogical-content-knowledge base, building on current pedagogical content knowledge. The findings of this study enhance the general understanding of the development and implementation of senior teachers regarding technology integration, as well as the integration of subject content and pedagogical knowledge in teaching.
Published: 2015

10. When Group Work Doesn't Work: Insights from Students

Author: Chang, Yunjeong and Brickman, Peggy
Abstract: Introducing group work in college science classrooms can lead to noticeable gains in student achievement, reasoning ability, and motivation. To realize these gains, students must all contribute. Strategies like assigning roles, group contracts, anonymous peer evaluations, and peer ratings all encourage student participation. In a class using these strategies, we conducted in-depth interviews to uncover student perceptions of group work in general and the utility of these support strategies. Students in both high- and low-performance groups still complained of unequal contributions while praising the social support provided by groups. Students who scored highly on tests were more likely to recognize the benefits of group work, regardless of their groups' overall performance levels, while lower-scoring students perceived group work as time-consuming "busy work" with little cognitive benefit. Comments from anonymous peer evaluations differed only subtly between high- and low-performance groups. Numerical ratings on these evaluations did correlate with overall group performance. However, students in lower-performance groups assigned harsh ratings to their low-scoring members, while students in higher-performance groups were more generous in their ratings for low-scoring members. We discuss implications of relying on support strategies for promoting productive group work.
Published: 2018
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11. Using Augmented Reality Technologies to Enhance Students' Engagement and Achievement in Science Laboratories

Author: Chang, Rong-Chi and Yu, Zeng-Shiang
Abstract: This article aims to explore the impact of AR on the learning outcomes of college freshmen and their knowledge about the biology lab course. In a study, a basic biology lab course app was developed using AR technology. The students used this app to prepare for a basic biology lab course, and carry on self-learning without the help of instructors. The results showed that by integrating AR technology into the instruction, the students took on a more positive autonomous learning attitude; they were able to gain a better grasp of the basic biology lab knowledge through the interactive operation as well as cooperative learning. In addition, the students were found to have learned the importance of scientific knowledge with this interactive technology. The basic biology lab course app developed in the study offered the benefits of autonomous learning, situational simulation and interactive experience.
Published: 2018
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12. Students' Communicative Resources in Relation to Their Conceptual Understanding--The Role of Non-Conventionalized Expressions in Making Sense of Visualizations of Protein Function

Author: Rundgren, Carl-Johan, Hirsch, Richard, and Chang Rundgren, Shu-Nu
Abstract: This study examines how students explain their conceptual understanding of protein function using visualizations. Thirteen upper secondary students, four tertiary students (studying chemical biology), and two experts were interviewed in semi-structured interviews. The interviews were structured around 2D illustrations of proteins and an animated representation of water transport through a channel in the cell membrane. In the analysis of the transcripts, a score, based on the SOLO-taxonomy, was given to each student to indicate the conceptual depth achieved in their explanations. The use of scientific terms and non-conventionalized expressions in the students' explanations were investigated based upon a semiotic approach. The results indicated that there was a positive relationship between use of scientific terms and level of education. However, there was no correlation between students' use of scientific terms and conceptual depth. In the interviews, we found that non-conventionalized expressions were used by several participants to express conceptual understanding and played a role in making sense of the visualizations of protein function. Interestingly, also the experts made use of non-conventionalized expressions. The results of our study imply that more attention should be drawn to students' use of scientific and non-conventionalized terms in relation to their conceptual understanding.
Published: 2012
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13. A Laboratory Exercise Illustrating the Sensitivity and Specificity of Western Blot Analysis

Author: Chang, Ming-Mei and Lovett, Janice
Abstract: Western blot analysis, commonly known as "Western blotting," is a standard tool in every laboratory where proteins are analyzed. It involves the separation of polypeptides in polyacrylamide gels followed by the electrophoretic transfer of the separated polypeptides onto a nitrocellulose or polyvinylidene fluoride membrane. A replica of the separated polypeptides from the gel is created on the membrane, which is then probed with antibodies or other ligands to identify specific polypeptide(s). Here, we report an undergraduate laboratory exercise involving Western blotting. During the 3-week laboratory exercise, students investigated the likelihood of the presence of a serum albumin in fruit fly ("Drosophila melanogaster") homogenate, bovine calf serum (BCS), and fetal bovine serum (FBS). Students isolate proteins from fruit fly larvae and perform sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), electrophoretic blotting, and immunoassay comparing those proteins with BCS and FBS proteins. Their results indicate that serum albumin is present in BCS and FBS but is absent in fruit flies. In the process, the specificity and sensitivity of Western blot analysis is demonstrated. The laboratory exercise can be easily incorporated into any college-level biochemistry or molecular techniques laboratory. The procedure used can be easily adapted to study other proteins. (Contains 3 figures.)
Published: 2011
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14. Students' Conceptions of Water Transport

Author: Rundgren, Carl-Johan, Rundgren, Shu-Nu Chang, and Schonborn, Konrad J.
Abstract: Understanding diffusion of water into and out of the cell through osmosis is fundamental to the learning and teaching of biology. Although this process is thought of as occurring directly across the lipid bilayer, the majority of water transport is actually mediated by specialised transmembrane water-channels called aquaporins. This study investigated a total of 175 Taiwanese and Swedish students' conceptions of water transport across the cell membrane, and at what level of biological organisation students represented such knowledge. A free response instrument was employed to generate students' written and diagrammatic responses. Analysis of the data resulted in the emergence of four distinct categorisations of students' conceptions. Corresponding analysis of students' diagrams revealed varying levels of scale for representing and expressing water transport conceptions. The results revealed that many students lacked an awareness of the role of specialised water channels in water transport and thought that the majority of water molecules traversed the hydrophobic membrane through direct diffusion. Our findings suggest that teaching the topic of diffusion and osmosis be aligned with current scientific discovery. (Contains 4 figures and 1 table.)
Published: 2010
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15. Learning from Writing in College Biology

Author: Armstrong, Norris A., Wallace, Carolyn S., and Chang, Shu-Mei
Abstract: This study used both quantitative and qualitative analyses to examine the influence of written arguments on learning in a college level introductory biology class and the types of metacognition employed by students while writing. Comparison of a treatment and control group indicates that the writing assignments used had minimal impact on overall content learning as measured by conventional exams. Subsequent interviews and think-aloud protocols with a subset of students indicated that writing arguments had the potential to foster learning through forward and backward search strategies. However, few of the students took advantage of this opportunity to use metacognitive skills. This study suggests that preparing written arguments is not sufficient, by itself, to have a reliable effect on student learning and is consistent with the view that students must be explicitly taught when and how to use different metacognitive strategies.
Published: 2008
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16. Location, Location, Location: Does Seat Location Affect Performance in Large Classes?

Author: National Science Teachers Association, Arlington, VA., Armstrong, Norris, and Chang, Shu-Mei
Abstract: This study found no evidence that seat location affects student achievement even in very large classes where students sitting in the back are a considerable distance from the instructor. Rather, the differences in performance observed were consistent with the idea that highly motivated students are more likely to sit in the front, resulting in an uneven distribution of scores. (Contains 1 figure and 1 table.)
Published: 2007
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17. A Study on the Factors Affecting Biological Concept Learning of Junior High School Students

Author: Wang, Min-Nan Maynard, Wu, Kun-Chang, and Huang, Tai-Chu Iris
Abstract: The purpose of this study is to investigate how factors such as personal traits and school locations influence junior high school students' conceptual learning of biology. The study was carried out island-wide with the whole area divided into 10 districts, from which 4,537 students were selected using stratified random sampling. A questionnaire on biological concepts was used to gather the data, which then were statistically analysed using one-way analysis of variance and a t-test. It was found that junior high school students in urban areas had clearer and better biological concepts than those of students in eastern Taiwan and other distant districts. Students in the ninth grade performed better than those in the eighth grade. No significant difference was observed in terms of gender. Besides, students who had more positive biology self-efficacy, who watched science-oriented television programmes, and who had stronger motivation and more positive attitudes towards biology learning all scored higher for their biological concepts. On the basis of the findings of the study, the authors make several suggestions for improving biology education. (Contains 7 tables.)
Published: 2007
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18. Cooperative Learning in Industrial-Sized Biology Classes

Author: Armstrong, Norris, Chang, Shu-Mei, and Brickman, Marguerite
Abstract: This study examined the impact of cooperative learning activities on student achievement and attitudes in large-enrollment (greater than 250) introductory biology classes. We found that students taught using a cooperative learning approach showed greater improvement in their knowledge of course material compared with students taught using a traditional lecture format. In addition, students viewed cooperative learning activities highly favorably. These findings suggest that encouraging students to work in small groups and improving feedback between the instructor and the students can help to improve student outcomes even in very large classes. These results should be viewed cautiously, however, until this experiment can be replicated with additional faculty. Strategies for potentially improving the impact of cooperative learning on student achievement in large courses are discussed. (Contains 4 tables and 1 figure.)
Published: 2007
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19. Externalising Students' Mental Models through Concept Maps

Author: Chang, Shu-Nu
Abstract: The purpose of this study is to use concept maps as an "expressed model" to investigate students' mental models regarding the homeostasis of blood sugar. The difficulties in learning the concept of homeostasis and in probing mental models have been revealed in many studies. Homeostasis of blood sugar is one of the themes in junior high school biology in Taiwan. However, few studies have investigated it. Here, semi-structured interviews were conducted together with concept mapping to explore students' mental models. There were 14 students, 7th- and 9th-graders, in this study. From the results, both groups were shown to lack a concept of the nervous system. However, 7th-graders did have more developed concepts of the endocrine and digestive systems. The data also indicated that students used external behaviours or life experiences to explain the mechanism responsible for the homeostasis of blood sugar, but did not possess a detailed view of the components of the mechanism. The implications, and recommendations for pedagogy, are discussed. (Contains 5 figures and 1 table.)
Published: 2007
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20. Analysis of Korean High School Students' Decision-Making Processes in Solving a Problem Involving Biological Knowledge

Author: Hong, Jung-Lim and Chang, Nam-Kee
Abstract: In this study, the cognitive characteristics of students' decision-making processes centered on phases, difficulties, and strategies are analysed in the personal dailylife context involving biological knowledge. The subjects were first year science and general high school students in Seoul, Korea; 6 female students and 7 male students. The students' decision-making processes were analysed by "think-aloud" and participant observation methods. On the whole, the students' decision-making processes progressed in following order: recognizing a problem, searching for alternatives, evaluating the alternatives, and decision. During the decision-making processes, the above phases were repeated by trial and error. Students preferred noncompensatory rules that did not allow trade offs among alternatives for decisions, rather than compensatory rules of selection. Students had a tendency to have difficulties in analysing the difference between initial state and desirable state of the problem, organising biological knowledge-related problems, and clarifying values as selective criteria. Even students who had high achievement and more positive science-related attitudes did not apply biological knowledge to search for alternatives, and could not utilise scientific values as selective criteria very well. We discuss the implications of these results for teaching of decision-making in respect to scientific literacy.
Published: 2004
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21. Science Curriculum Components Favoured by High School Students in Taiwan

Author: Hu, Reping, Chang, Wen-Hua, and Lin, Chen-Yung
Abstract: This study examines the curriculum components in biology favoured by high school students in Taiwan. Our sample consisted of 155 senior high school students (51 boys and 104 girls) and 137 junior high school students (74 boys and 63 girls). The questionnaire was based on the idea of a repertory grid technique and was developed to investigate the relative status of the curriculum components as perceived by the students. Descriptive statistics and t-tests were then used to analyse the data. It was found that the students favoured the curriculum components in the following order: Manipulative skills, Scientific concepts, the Application of science, Social issues, Problem solving skills and the History of science. Further comparisons indicated that there were some differences between senior high school students and junior high school students as well as between boys and girls. These findings provide us with aspects that should be seriously considered when developing curricula. (Contains 6 tables and 4 figures.)
Published: 2003
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22. The Use of Internet-Based Learning in Biology.

Author: Lin, Chen-yung, Cheng, Yeong-jing, Chang, Yung-ta, and Hu, Reping
Abstract: Describes a study that implemented an Internet-based project in a Taiwanese secondary school biology class and investigated its effect on the cognitive preferences held by students and on their performance. Reports results of analyses of covariance that compared scores of short-answer questions, multiple choice items, and total final examination scores. (Author/LRW)
Published: 2002

23. Learning through Computer-based Concept Mapping with Scaffolding Aid.

Author: Chang, K. E., Sung, Y. T., and Chen, S. F.
Abstract: A computer-based concept mapping system was developed that provides two learning environments: "construct-by-self," where the system provides students with evaluation results and hints for feedback, and "construct-on-scaffold," where students also receive an incomplete concept map. The "construct-on-scaffold" had better effect for learning biology. Both are helpful for students completing their concept maps. (Author/AEF)
Published: 2001

24. Connecting To Increase Task Variety in Biology Classroom--A Case Study.

Author: Chang, Wen-Hua
Abstract: This study explores the impact of a researcher-developed World Wide Web database on a variety of tasks and talk in a science classroom. The study was part of an experienced teacher's action research project designed to increase student engagement in biology class. Lessons were videotaped approximately once a week throughout the entire school year, written materials were collected, and the teacher was interviewed at regular intervals. The teacher employed a wide variety of task designs applying strategies such as concept mapping, group investigation, games and tournaments, and models such as the learning cycle and Driver's 5-stage conceptual teaching model. The teacher-student interactions that evolved from whole-class discussions were found to differ in the ownership of scientific talk. (Contains 10 references.) (Author/WRM)
Published: 1999

25. A Study of Korean Middle School Students' Interests in Biology and Their Implications for Biology Education.

Author: Hong, Jung-Lim, Chang, Nam-Kee, and Shim, Kew-Cheol
Abstract: Analyzes middle school students' interests in biology to provide information on instructional strategies and curriculum development in Korea. Reports that the highest interest domains are genetics and microscope observations. Contains 16 references. (DDR)
Published: 1998

26. SMARCA4 biology in alveolar rhabdomyosarcoma

Author: Bharathy, Narendra, Cleary, Megan M, Kim, Jin-Ah, Nagamori, Kiyo, Crawford, Kenneth A, Wang, Eric, Saha, Debarya, Settelmeyer, Teagan P, Purohit, Reshma, Skopelitis, Damianos, Chang, Kenneth, Doran, Jessica A, Kirschbaum, C Ward, Bharathy, Suriya, Crews, Davis W, Randolph, Matthew E, Karnezis, Anthony N, Hudson-Price, Lisa, Dhawan, Jyotsna, Michalek, Joel E, Ciulli, Alessio, Vakoc, Christopher R, and Keller, Charles
Subjects: Biomedical and Clinical Sciences, Oncology and Carcinogenesis, Cancer, Rare Diseases, Pediatric Research Initiative, Genetics, Pediatric Cancer, Orphan Drug, Pediatric, Aetiology, 2.1 Biological and endogenous factors, Biology, Child, DNA Helicases, Humans, Neoplasms, Nuclear Proteins, Rhabdomyosarcoma, Alveolar, Rhabdomyosarcoma, Embryonal, Transcription Factors, Clinical Sciences, Oncology & Carcinogenesis, Biochemistry and cell biology, Oncology and carcinogenesis
Abstract: Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children and phenocopies a muscle precursor that fails to undergo terminal differentiation. The alveolar subtype (ARMS) has the poorest prognosis and represents the greatest unmet medical need for RMS. Emerging evidence supports the role of epigenetic dysregulation in RMS. Here we show that SMARCA4/BRG1, an ATP-dependent chromatin remodeling enzyme of the SWI/SNF complex, is prominently expressed in primary tumors from ARMS patients and cell cultures. Our validation studies for a CRISPR screen of 400 epigenetic targets identified SMARCA4 as a unique factor for long-term (but not short-term) tumor cell survival in ARMS. A SMARCA4/SMARCA2 protein degrader (ACBI-1) demonstrated similar long-term tumor cell dependence in vitro and in vivo. These results credential SMARCA4 as a tumor cell dependency factor and a therapeutic target in ARMS.
Published: 2022

27. IMRT and SBRT Treatment Planning Study for the First Clinical Biology-Guided Radiotherapy System

Author: Pham, Daniel, Simiele, Eric, Breitkreutz, Dylan, Capaldi, Dante, Han, Bin, Surucu, Murat, Oderinde, Seyi, Vitzthum, Lucas, Gensheimer, Michael, Bagshaw, Hilary, Chin, Alex, Xing, Lei, Chang, DT, and Kovalchuk, Natalyia
Subjects: Biomedical and Clinical Sciences, Clinical Sciences, Oncology and Carcinogenesis, Cancer, Biology, Humans, Male, Organs at Risk, Radiosurgery, Radiotherapy Dosage, Radiotherapy Planning, Computer-Assisted, Radiotherapy, Intensity-Modulated, Retrospective Studies, Dosimetry, radiation dosimetry, stereotactic body radiation therapy, stereotactic radiosurgery, stereotactic radiotherapy, Oncology & Carcinogenesis, Oncology and carcinogenesis
Abstract: Purpose: The first clinical biology-guided radiation therapy (BgRT) system-RefleXionTM X1-was installed and commissioned for clinical use at our institution. This study aimed at evaluating the treatment plan quality and delivery efficiency for IMRT/SBRT cases without PET guidance. Methods: A total of 42 patient plans across 6 cancer sites (conventionally fractionated lung, head, and neck, anus, prostate, brain, and lung SBRT) planned with the EclipseTM treatment planning system (TPS) and treated with either a TrueBeam® or Trilogy® were selected for this retrospective study. For each Eclipse VMAT plan, 2 corresponding plans were generated on the X1 TPS with 10 mm jaws (X1-10mm) and 20 mm jaws (X1-20mm) using our institutional planning constraints. All clinically relevant metrics in this study, including PTV D95%, PTV D2%, Conformity Index (CI), R50, organs-at-risk (OAR) constraints, and beam-on time were analyzed and compared between 126 VMAT and RefleXion plans using paired t-tests. Results: All but 3 planning metrics were either equivalent or superior for the X1-10mm plans as compared to the Eclipse VMAT plans across all planning sites investigated. The Eclipse VMAT and X1-10mm plans generally achieved superior plan quality and sharper dose fall-off superior/inferior to targets as compared to the X1-20mm plans, however, the X1-20mm plans were still considered acceptable for treatment. On average, the required beam-on time increased by a factor of 1.6 across all sites for X1-10mm compared to X1-20mm plans. Conclusions: Clinically acceptable IMRT/SBRT treatment plans were generated with the X1 TPS for both the 10 mm and 20 mm jaw settings.
Published: 2022

28. Memories Are Made of This

Author: Chang, Christine
Abstract: In this article, the author shares her memories of Sally Smith, the founder of The Lab School of Washington, where she works as the director of the Occupational Therapy. When the author first met Smith, Smith asked her what brought her to The Lab School at that point in her career. She told Smith that her background was rather eclectic, since she had worked for many years in jobs not directly connected with Occupational Therapy. She later realized that it was the richness of having faculty with a broad variety of life experiences that she particularly liked. However, a very sad time for the Lab School family was the passing its fearless leader, yet it was also a learning time as well. At a personal level, the author still hears Smith in her mind saying that the priority must be the children, that learning that is fun will stick with a teacher longer, and finally she hears her challenge her thinking with, "Why not?" The author likes to think Smith would be pleased to see that everyone still feels her presence around every corner and that everyone remains up to the challenge of meeting her creative expectations for the futures of the students.
Published: 2010

29. Voices of chemical biology.

Author: Bertozzi, Carolyn R, Stockwell, Brent R, Kubicek, Stefan, Dickinson, Bryan C, Chang, Christopher J, Schultz, Carsten, Silver, Pamela A, Gestwicki, Jason E, Chiosis, Gabriela, Chattopadhyay, Amitabha, Butcher, Rebecca A, Park, Seung Bum, Shoichet, Brian K, Flitsch, Sabine L, Zhang, Jin, Liu, David R, Ohnishi, Yasuo, Weerapana, Eranthie, Williams, Allison H, He, Chuan, Moroni, Anna, Thiel, Gerhard, Chang, Young-Tae, Waldmann, Herbert, Bogyo, Matthew, Oddershede, Lene B, Christopoulos, Arthur, Imperiali, Barbara, Ehrlich, Hermann, Chen, Xing, and Prescher, Jennifer A
Subjects: Interdisciplinary Communication, Biology, Chemistry, Biochemistry & Molecular Biology, Medicinal and Biomolecular Chemistry, Biochemistry and Cell Biology
Published: 2015

30. Learn biology using digital game-based learning: A systematic literature review.

Author: Situmorang, Risya Pramana, Suwono, Hadi, Munzil, Susanto, Hendra, Chun-Yen Chang, and Shan-Yu Liu
Subjects: BIOLOGY education, EDUCATIONAL games, DIGITAL technology, COURSEWARE, SOCIAL interaction
Abstract: The implementation of digital games in biology education has steadily increased over the past decade. The utilization of simulation-based learning tools, such as digital games, is anticipated to be further enhanced due to the increasing shift toward virtual learning. This review aims to explore the existing scientific evidence and analyze the effectiveness of incorporating digital games as a learning tool in biology education. The research methodology was a systematic literature review of existing digital game-based learning (DGBL) in biology education. The term "digital gamebased learning and education" was utilized in the search interface of the Scopus database, identifying 1,241 articles. However, only 56 articles adhered to the stipulated criteria for subsequent analysis. Preferred reporting items for systematic reviews and meta-analyses framework was employed for the process of inclusion and exclusion. A discernible trend of escalating publications has been observed over the past decade. The review findings indicate that DGBL has expanded widely, encompassing all levels of education, from elementary to higher education, and has even been applied in professional education. Researchers primarily utilize quantitative research methods, although qualitative methods are employed. This research found that the complexity of DGBL research requires interdisciplinary collaboration involving science, biomedical, education, and technology experts. In terms of content, DGBL has been used to teach students about basic biology and practical applications of biology, such as biomedical science. Genetics, environment, and healthcare emerged as the most frequently addressed subjects taught using DGBL. Trends in DGBL technology usage include various platforms like computer-based, internet-based, and mobile-based, with elements of gaming and social interaction that align with the demands of 21st century skills. DGBL integrates with several constructivist learning approaches like experiential learning, problem-based, inquiry-based, and inquiry-lab. Research showed that DGBL encourages development of 21st century skills in simulated settings and real-life situations. [ABSTRACT FROM AUTHOR]
Published: 2024
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31. Functional Characterization of Bioactive Lipids and Microproteins

Author: Chang, Tina Chia-Tien
Subjects: Biology, cristae, MICOS, microprotein, mitochondria, upstream open reading frame
Abstract: Lipids and proteins are essential natural small molecules that serve as the building blocks of eukaryotic cells. They play vital roles in regulating nearly all biological processes, including stress adaptation, development and growth, metabolism, and regeneration. An intricate interplay among these molecules facilitates proper functioning of cells and their machinery critical to human health. A disruption in their homeostasis would likely alter pathways that mediate physiology, leading to deleterious human diseases.For decades, a lack of available detection and experimental tools hindered our understanding of natural small molecules and their biology. It was not until recent technologies that led to groundbreaking discoveries of beneficial lipids called FAHFAs and protein-coding small open reading frames (smORFs). The identifications of these previously uncharacterized small natural molecules highlight that the current capacities of mammalian lipidome and transcriptome have been underestimated. Thus, this dissertation will focus on our efforts to functionally characterize these newly identified molecules and their biological roles.Recent discovery of a new bioactive lipid class called branched fatty acid esters of hydroxy fatty acids (FAHFAs) demonstrated great structural diversity, with some, such as palmitic acid 9-hydroxy stearic acid (9-PAHSA), exhibiting inflammation-reducing effects. While over 80 distinct FAHFA isomers have been reported, the biological activities among these lipid species are poorly defined. Because the PAHSAs were previously demonstrated to mediate inflammation in murine and human disease models, we investigated if other endogenous FAHFAs with more potent biological activities than PAHSAs exist in nature. We present that oat oil is a natural source rich of FAHFAs. Analysis of human serum following ingestion of liposomes consisted of fractionated oat oil revealed linoleic acid esters of hydroxy linoleic acids (LAHLAs) as a new FAHFA family, with 13-LAHLA being the most abundant LAHLA isomer. Comparison of 13-LAHLA and 9-PAHSA’s ability to attenuate LPS-stimulated cytokine release under acute inflammation in RAW264.7 macrophages demonstrates that 13-LAHLA was more active than 9-PAHSA, implying a structural-activity relationship (SAR) exists within the lipid class. Characterization of FAHFAs can be difficult due to their susceptibility to endogenous metabolism. FAHFAs are not only subjected to hydrolysis by FAHFA-specific lipases, such as CEL, AIG1 and ADTRP, they can also be incorporated to form FAHFA-containing triglycerols (FAHFA-TGs). While these pathways reflect that FAHFAs are dynamically regulated, metabolism of FAHFAs could diminish their beneficial effects in animal physiology. Therefore, we hypothesized that synthetic FAHFAs with greater stability could protect these derivates from endogenous degradation, thus prolonging their systemic levels and favorable effects. Syntheses and activity screening of a library of synthetic FAHFA derivatives nominated a number of selective potent regulators of inflammation. Subsequent testing of the lead compound in a mouse model of colitis confirms its anti-inflammatory activity and pharmacokinetics profile, potentiating a new non-steroidal therapeutic option against inflammation for this small molecule. In addition to FAHFAs, smORFs belong to a group of underexplored natural small molecules with translational potential. For decades, translation of eukaryotic genes strictly followed a monocistronic model such that translation would only occur once per transcript and only at the longest ORF found within the transcript. This prevented annotation of smaller transcripts like smORF. Recently, the advent ribosome profiling has transformed the understanding of eukaryotic transcriptomes by revealing that many transcripts are polycistronic and smORFs can encode functional microproteins critical to biology. Among smORFs, upstream ORFs (uORFs) are found in at least half of the human transcriptome, representing the largest type of smORFs. Relative to their prevalence, evidence supporting uORF-encoded microproteins remains extremely limited. uORFs are primarily known as cis-regulating elements in the transcriptome, where they mediate the translation of their downstream canonical transcript. However, the notable example of MIEF, a microprotein encoded by the uORF of MIEF1 gene and a regulator of mitochondrial translation, exemplifies that microproteins with independent functions can also arise from uORFs. This raises the possibility that the eukaryotic transcriptome is an abundant repository for many more unique uORF-encoded microproteins. Therefore, we selected the SLC35A4 uORF with previously demonstrated cis-regulating roles and functionally characterized its microprotein product (SLC35A4-MP). Here, we report that SLC35A4-MP is a bona fide member of the mitochondrial contact site and cristae organizing system (MICOS) complex, a protein complex essential for mitochondrial crista formation and mitochondrial respiration. Loss of SLC35A4-MP disrupts mitochondrial crista and crista junction integrity and impaired the optimal oxidative capacities of cells. This finding demonstrates that new functional members of vital protein complexes can be extracted from the abundant reservoir of uncharacterized uORF-encoded microprotein. As additional discoveries of natural small molecules are underway, we believe that our data provides an incentive for their further evaluation. Through development of systematic approaches to interrogate the fundamental biology of lipids and proteins, we aim to provide a template on how one can functionally characterize natural small molecules to counteract the molecular basis of human diseases. Our goal is to gain deeper knowledge of the mammalian lipidome and transcriptome, ultimately leading our path to designing better treatments.
Published: 2022

32. A Single-Cell Transcriptomic Profiling of the Effects of House Dust Mite Exposure on the Murine Lung Microenvironment

Author: Chang, Han
Subjects: Biology, asthma, HDM, IL-1β, lung cancer, lung microenvironment, scRNA-seq
Abstract: House Dust Mite (HDM) is a common aeroallergen that can induce asthma and allergic airway inflammation. The effects of acute exposure to HDM have been well studied in various mouse models asthma; however, the effects of chronic exposure have been much less investigated. Our studies identified that chronic exposure to HDM induces chronic lung inflammation and accelerates lung cancer development in two different mouse models of lung cancer. The lung cancer-promoting effect of HDM was mainly due to chronic activation of the NLRP3 inflammasome in lung macrophages and persistent production of IL-1β in the lungs. Based on these findings, we hypothesize that chronic exposure to HDM changes the lung microenvironment and makes it conducive to tumor growth by activating the IL-1β signaling pathway. To further evaluate the mechanisms by which HDM affects the lung microenvironment, we conducted a single-cell RNA sequencing (scRNA-seq) analysis and compared the effect of chronic HDM exposure in the lungs of wild-type (WT) and IL-1β knock out (KO) mice. Our findings suggest that HDM and IL-1β signaling affect various cell types such as macrophage, neutrophils, and T cells and their gene profiles that may contribute to the protumorigenic effects of HDM and IL-1β in the lungs. In conclusion, our study revealed the transcriptomic landscape of the murine lung microenvironment and uncovered the effects of chronic HDM exposure and IL-1β signaling.
Published: 2022

33. Changes in the Biology and Susceptibility of Weevil (Cylas formicarius) to the Insecticide Spinetoram as a Response to Cadmium Contamination.

Author: Xu, Jin, Tang, An, Liu, Jun-Yan, Yao, Chang-Long, Liu, Ke-Ping, Huang, Xiao-Sheng, and Shi, Pei-Qiong
Subjects: PESTICIDE resistance, CURCULIONIDAE, SWEET potatoes, CADMIUM, INSECTICIDES, BIOLOGY
Abstract: The sweet potato weevil Cylas formicarius is a notorious underground pest in sweet potato (Ipomoea batatas L.). However, little is known about the effects of cadmium (Cd) stress on weevil biology and resistance to pesticides and biotic agents. Therefore, we fed sweet potato weevils with Cd-contaminated sweet potato and assessed adult food intake and survival and larval developmental duration and mortality rates, as well as resistance to the insecticide spinetoram and susceptibility to the entomopathogenic fungus Beauveria bassiana. With increasing Cd concentration, the number of adult weevil feeding holes, adult survival and life span, and larval developmental duration decreased significantly, whereas larval mortality rates increased significantly. However, at the lowest Cd concentration (30 mg/L), adult feeding was stimulated. Resistance of adult sweet potato weevils to spinetoram increased at low Cd concentration, whereas Cd contamination did not affect sensitivity to B. bassiana. Thus, Cd contamination affected sweet potato weevil biology and resistance, and further studies will investigate weevil Cd accumulation and detoxification mechanisms. [ABSTRACT FROM AUTHOR]
Published: 2024
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34. Modulation of gut microbiota by foods and herbs to prevent cardiovascular diseases

Author: Chi-Tang Ho, Chieh Chang Chen, Wei-Kai Wu, Ming-Shiang Wu, Suraphan Panyod, and Lee-Yan Sheen
Subjects: medicine.medical_specialty, biology, Synbiotics, business.industry, digestive, oral, and skin physiology, Disease, Gut flora, Bioinformatics, biology.organism_classification, digestive system, Gut microbiome, Complementary and alternative medicine, Medicine, Dietary nutrients, Dietary therapy, Adverse effect, business, Preventive healthcare
Abstract: Dietary nutrients are associated with the development of cardiovascular disease (CVD) both through traditional pathways (inducing hyperlipidemia and chronic inflammation) and through the emergence of a metaorganism-pathogenesis pathway (through the gut microbiota, its metabolites, and host). Several molecules from food play an important role as CVD risk-factor precursors either themselves or through the metabolism of the gut microbiome. Animal-based dietary proteins are the primary source of CVD risk-factor precursors; however, some plants also possess these precursors, though at relatively low levels compared with animal-source food products. Various medications have been developed to treat CVD through the gut-microbiota–circulation axis, and they exhibit potent effects in CVD treatment. Nevertheless, such medicines are still being improved, and there are many research gaps that need to be addressed. Furthermore, some medications have unpleasant or adverse effects. Numerous foods and herbs impart beneficial effects upon health and disease. In the past decade, many studies have focused on treating and preventing CVD by modulating the gut microbiota and their metabolites. This review provides an overview of the available information, summarizes current research related to the gut-microbiota–heart axis, enumerates the foods and herbs that are CVD-risk precursors, and illustrates how metabolites become CVD risk factors through the metabolism of gut microbiota. Moreover, we present perspectives on the application of foods and herbs—including prebiotics, probiotics, synbiotics, postbiotics, and antibiotic-like substances—as CVD prevention agents to modulate gut microbiota by inhibiting gut-derived CVD risk factors. Taxonomy (classification by EVISE) Cardiovascular disease, gut microbiota, herbal medicine, preventive medicine, dietary therapy, nutrition supplements.
Published: 2023

35. A first update on mapping the human genetic architecture of COVID-19

Author: COVID-19 Host Genetics Initiative, Pathak, GA, Karjalainen, J, Stevens, C, Neale, BM, Daly, M, Ganna, A, Andrews, SJ, Kanai, M, Cordioli, M, Polimanti, R, Harerimana, N, Pirinen, M, Liao, RG, Chwialkowska, K, Trankiem, A, Balaconis, MK, Nguyen, H, Solomonson, M, Veerapen, K, Wolford, B, Roberts, G, Park, D, Ball, CA, Coignet, M, McCurdy, S, Knight, S, Partha, R, Rhead, B, Zhang, M, Berkowitz, N, Gaddis, M, Noto, K, Ruiz, L, Pavlovic, M, Hong, EL, Rand, K, Girshick, A, Guturu, H, Baltzell, AH, Niemi, MEK, Rahmouni, S, Guntz, J, Beguin, Y, Pigazzini, S, Nkambule, L, Georges, M, Moutschen, M, Misset, B, Darcis, G, Guiot, J, Azarzar, S, Gofflot, S, Claassen, S, Malaise, O, Huynen, P, Meuris, C, Thys, M, Jacques, J, Leonard, P, Frippiat, F, Giot, J-B, Sauvage, A-S, Frenckell, CV, Belhaj, Y, Lambermont, B, Nakanishi, T, Morrison, DR, Mooser, V, Richards, JB, Butler-Laporte, G, Forgetta, V, Li, R, Ghosh, B, Laurent, L, Belisle, A, Henry, D, Abdullah, T, Adeleye, O, Mamlouk, N, Kimchi, N, Afrasiabi, Z, Rezk, N, Vulesevic, B, Bouab, M, Guzman, C, Petitjean, L, Tselios, C, Xue, X, Afilalo, J, Afilalo, M, Oliveira, M, Brenner, B, Brassard, N, Durand, M, Schurr, E, Lepage, P, Ragoussis, J, Auld, D, Chassé, M, Kaufmann, DE, Lathrop, GM, Adra, D, Hayward, C, Glessner, JT, Shaw, DM, Campbell, A, Morris, M, Hakonarson, H, Porteous, DJ, Below, J, Richmond, A, Chang, X, Polikowski, H, Lauren, PE, Chen, H-H, Wanying, Z, Fawns-Ritchie, C, North, K, McCormick, JB, Glessner, JR, Gignoux, CR, Wicks, SJ, Crooks, K, Barnes, KC, Daya, M, Shortt, J, Rafaels, N, Chavan, S, Timmers, PRHJ, Wilson, JF, Tenesa, A, Kerr, SM, D’Mellow, K, Shahin, D, El-Sherbiny, YM, von Hohenstaufen, KA, Sobh, A, Eltoukhy, MM, Nkambul, L, Elhadidy, TA, Abd Elghafar, MS, El-Jawhari, JJ, Mohamed, AAS, Elnagdy, MH, Samir, A, Abdel-Aziz, M, Khafaga, WT, El-Lawaty, WM, Torky, MS, El-shanshory, MR, Yassen, AM, Hegazy, MAF, Okasha, K, Eid, MA, Moahmed, HS, Medina-Gomez, C, Ikram, MA, Uitterlinden, AG, Mägi, R, Milani, L, Metspalu, A, Laisk, T, Läll, K, Lepamets, M, Esko, T, Reimann, E, Naaber, P, Laane, E, Pesukova, J, Peterson, P, Kisand, K, Tabri, J, Allos, R, Hensen, K, Starkopf, J, Ringmets, I, Tamm, A, Kallaste, A, Alavere, H, Metsalu, K, Puusepp, M, Batini, C, Tobin, MD, Venn, LD, Lee, PH, Shrine, N, Williams, AT, Guyatt, AL, John, C, Packer, RJ, Ali, A, Free, RC, Wang, X, Wain, LV, Hollox, EJ, Bee, CE, Adams, EL, Palotie, A, Ripatti, S, Ruotsalainen, S, Kristiansson, K, Koskelainen, S, Perola, M, Donner, K, Kivinen, K, Kaunisto, M, Rivolta, C, Bochud, P-Y, Bibert, S, Boillat, N, Nussle, SG, Albrich, W, Quinodoz, M, Kamdar, D, Suh, N, Neofytos, D, Erard, V, Voide, C, Friolet, R, Vollenweider, P, Pagani, JL, Oddo, M, zu Bentrup, FM, Conen, A, Clerc, O, Marchetti, O, Guillet, A, Guyat-Jacques, C, Foucras, S, Rime, M, Chassot, J, Jaquet, M, Viollet, RM, Lannepoudenx, Y, Portopena, L, Bochud, PY, Desgranges, F, Filippidis, P, Guéry, B, Haefliger, D, Kampouri, EE, Manuel, O, Munting, A, Papadimitriou-Olivgeris, M, Regina, J, Rochat-Stettler, L, Suttels, V, Tadini, E, Tschopp, J, Van Singer, M, Viala, B, Boillat-Blanco, N, Brahier, T, Hügli, O, Meuwly, JY, Pantet, O, Gonseth Nussle, S, Bochud, M, D’Acremont, V, Estoppey Younes, S, Albrich, WC, Cerny, A, O’Mahony, L, von Mering, C, Frischknecht, M, Kleger, G-R., Filipovic, M, Kahlert, CR, Wozniak, H, Negro, TR, Pugin, J, Bouras, K, Knapp, C, Egger, T, Perret, A, Montillier, P, di Bartolomeo, C, Barda, B, de Cid, R, Carreras, A, Moreno, V, Kogevinas, M, Galván-Femenía, I, Blay, N, Farré, X, Sumoy, L, Cortés, B, Mercader, JM, Guindo-Martinez, M, Torrents, D, Garcia-Aymerich, J, Castaño-Vinyals, G, Dobaño, C, Gori, M, Renieri, A, Mari, F, Mondelli, MU, Castelli, F, Vaghi, M, Rusconi, S, Montagnani, F, Bargagli, E, Franchi, F, Mazzei, MA, Cantarini, L, Tacconi, D, Feri, M, Scala, R, Spargi, G, Nencioni, C, Bandini, M, Caldarelli, GP, Canaccini, A, Ognibene, A, D’Arminio Monforte, A, Girardis, M, Antinori, A, Francisci, D, Schiaroli, E, Scotton, PG, Panese, S, Scaggiante, R, Monica, MD, Capasso, M, Fiorentino, G, Castori, M, Aucella, F, Biagio, AD, Masucci, L, Valente, S, Mandalà, M, Zucchi, P, Giannattasio, F, Coviello, DA, Mussini, C, Tavecchia, L, Crotti, L, Rizzi, M, Rovere, MTL, Sarzi-Braga, S, Bussotti, M, Ravaglia, S, Artuso, R, Perrella, A, Romani, D, Bergomi, P, Catena, E, Vincenti, A, Ferri, C, Grassi, D, Pessina, G, Tumbarello, M, Pietro, MD, Sabrina, R, Luchi, S, Furini, S, Dei, S, Benetti, E, Picchiotti, N, Sanarico, M, Ceri, S, Pinoli, P, Raimondi, F, Biscarini, F, Stella, A, Zguro, K, Capitani, K, Tanfoni, M, Fallerini, C, Daga, S, Baldassarri, M, Fava, F, Frullanti, E, Valentino, F, Doddato, G, Giliberti, A, Tita, R, Amitrano, S, Bruttini, M, Croci, S, Meloni, I, Mencarelli, MA, Rizzo, CL, Pinto, AM, Beligni, G, Tommasi, A, Sarno, LD, Palmieri, M, Carriero, ML, Alaverdian, D, Busani, S, Bruno, R, Vecchia, M, Belli, MA, Mantovani, S, Ludovisi, S, Quiros-Roldan, E, Antoni, MD, Zanella, I, Siano, M, Emiliozzi, A, Fabbiani, M, Rossetti, B, Bergantini, L, D’Alessandro, M, Cameli, P, Bennett, D, Anedda, F, Marcantonio, S, Scolletta, S, Guerrini, S, Conticini, E, Frediani, B, Spertilli, C, Donati, A, Guidelli, L, Corridi, M, Croci, L, Piacentini, P, Desanctis, E, Cappelli, S, Verzuri, A, Anemoli, V, Pancrazzi, A, Lorubbio, M, Miraglia, FG, Venturelli, S, Cossarizza, A, Vergori, A, Gabrieli, A, Riva, A, Paciosi, F, Andretta, F, Gatti, F, Parisi, SG, Baratti, S, Piscopo, C, Russo, R, Andolfo, I, Iolascon, A, Carella, M, Merla, G, Squeo, GM, Raggi, P, Marciano, C, Perna, R, Bassetti, M, Sanguinetti, M, Giorli, A, Salerni, L, Parravicini, P, Menatti, E, Trotta, T, Coiro, G, Lena, F, Martinelli, E, Mancarella, S, Gabbi, C, Maggiolo, F, Ripamonti, D, Bachetti, T, Suardi, C, Parati, G, Bottà, G, Domenico, PD, Rancan, I, Bianchi, F, Colombo, R, Barbieri, C, Acquilini, D, Andreucci, E, Segala, FV, Tiseo, G, Falcone, M, Lista, M, Poscente, M, Vivo, OD, Petrocelli, P, Guarnaccia, A, Baroni, S, van Heel, DA, Hunt, KA, Trembath, RC, Huang, QQ, Martin, HC, Mason, D, Trivedi, B, Wright, J, Finer, S, Akhtar, S, Anwar, M, Arciero, E, Ashraf, S, Breen, G, Chung, R, Curtis, CJ, Chowdhury, M, Colligan, G, Deloukas, P, Durham, C, Griffiths, C, Hurles, M, Hussain, S, Islam, K, Khan, A, Lavery, C, Lee, SH, Lerner, R, MacArthur, D, MacLaughlin, B, Martin, H, Miah, S, Newman, B, Safa, N, Tahmasebi, F, Griffiths, CJ, Smith, AV, Boughton, AP, Li, KW, LeFaive, J, Annis, A, Niavarani, A, Aliannejad, R, Sharififard, B, Amirsavadkouhi, A, Naderpour, Z, Tadi, HA, Aleagha, AE, Ahmadi, S, Moghaddam, SBM, Adamsara, A, Saeedi, M, Abdollahi, H, Hosseini, A, Chariyavilaskul, P, Jantarabenjakul, W, Hirankarn, N, Chamnanphon, M, Suttichet, TB, Shotelersuk, V, Pongpanich, M, Phokaew, C, Chetruengchai, W, Putchareon, O, Torvorapanit, P, Puthanakit, T, Suchartlikitwong, P, Nilaratanakul, V, Sodsai, P, Brumpton, BM, Hveem, K, Willer, C, Zhou, W, Rogne, T, Solligard, E, Åsvold, BO, Franke, L, Boezen, M, Deelen, P, Claringbould, A, Lopera, E, Warmerdam, R, Vonk, JM, van Blokland, I, Lanting, P, Ori, APS, Feng, Y-CA, Mercader, J, Weiss, ST, Karlson, EW, Smoller, JW, Murphy, SN, Meigs, JB, Woolley, AE, Green, RC, Perez, EF, Zöllner, S, Wang, J, Beck, A, Sloofman, LG, Ascolillo, S, Sebra, RP, Collins, BL, Levy, T, Buxbaum, JD, Sealfon, SC, Jordan, DM, Thompson, RC, Gettler, K, Chaudhary, K, Belbin, GM, Preuss, M, Hoggart, C, Choi, S, Underwood, SJ, Salib, I, Britvan, B, Keller, K, Tang, L, Peruggia, M, Hiester, LL, Niblo, K, Aksentijevich, A, Labkowsky, A, Karp, A, Zlatopolsky, M, Zyndorf, M, Charney, AW, Beckmann, ND, Schadt, EE, Abul-Husn, NS, Cho, JH, Itan, Y, Kenny, EE, Loos, RJF, Nadkarni, GN, Do, R, O’Reilly, P, Huckins, LM, Ferreira, MAR, Abecasis, GR, Leader, JB, Cantor, MN, Justice, AE, Carey, DJ, Chittoor, G, Josyula, NS, Kosmicki, JA, Horowitz, JE, Baras, A, Gass, MC, Yadav, A, Mirshahi, T, Hottenga, JJ, Bartels, M, de geus, EEJC, Nivard, MMG, Verma, A, Ritchie, MD, Rader, D, Li, B, Verma, SS, Lucas, A, Bradford, Y, Abedalthagafi, M, Alaamery, M, Alshareef, A, Sawaji, M, Massadeh, S, AlMalik, A, Alqahtani, S, Baraka, D, Harthi, FA, Alsolm, E, Safieh, LA, Alowayn, AM, Alqubaishi, F, Mutairi, AA, Mangul, S, Almutairi, M, Aljawini, N, Albesher, N, Arabi, YM, Mahmoud, ES, Khattab, AK, Halawani, RT, Alahmadey, ZZ, Albakri, JK, Felemban, WA, Suliman, BA, Hasanato, R, Al-Awdah, L, Alghamdi, J, AlZahrani, D, AlJohani, S, Al-Afghani, H, AlDhawi, N, AlBardis, H, Alkwai, S, Alswailm, M, Almalki, F, Albeladi, M, Almohammed, I, Barhoush, E, Albader, A, Alotaibi, S, Alghamdi, B, Jung, J, fawzy, MS, Alrashed, M, Zeberg, H, Frithiof, R, Hultström, M, Lipcsey, M, Tardif, N, Rooyackers, O, Grip, J, Maricic, T, Helgeland, Ø, Magnus, P, Trogstad, L-IS, Lee, Y, Harris, JR, Mangino, M, Spector, TD, Emma, D, Moutsianas, L, Caulfield, MJ, Scott, RH, Kousathanas, A, Pasko, D, Walker, S, Stuckey, A, Odhams, CA, Rhodes, D, Fowler, T, Rendon, A, Chan, G, Arumugam, P, Karczewski, KJ, Martin, AR, Wilson, DJ, Spencer, CCA, Crook, DW, Wyllie, DH, O’Connell, AM, Atkinson, EG, Tsuo, K, Baya, N, Turley, P, Gupta, R, Walters, RK, Palmer, DS, Sarma, G, Cheng, N, Lu, W, Churchhouse, C, Goldstein, JI, King, D, Seed, C, Daly, MJ, Finucane, H, Bryant, S, Satterstrom, FK, Band, G, Earle, SG, Lin, S-K, Arning, N, Koelling, N, Armstrong, J, Rudkin, JK, Callier, S, Cusick, C, Soranzo, N, Zhao, JH, Danesh, J, Angelantonio, ED, Butterworth, AS, Sun, YV, Huffman, JE, Cho, K, O’Donnell, CJ, Tsao, P, Gaziano, JM, Peloso, G, Ho, Y-L, Smieszek, SP, Polymeropoulos, C, Polymeropoulos, V, Polymeropoulos, MH, Przychodzen, BP, Fernandez-Cadenas, I, Planas, AM, Perez-Tur, J, Llucià-Carol, L, Cullell, N, Muiño, E, Cárcel-Márquez, J, DeDiego, ML, Iglesias, LL, Soriano, A, Rico, V, Agüero, D, Bedini, JL, Lozano, F, Domingo, C, Robles, V, Ruiz-Jaén, F, Márquez, L, Gomez, J, Coto, E, Albaiceta, GM, García-Clemente, M, Dalmau, D, Arranz, MJ, Dietl, B, Serra-Llovich, A, Soler, P, Colobrán, R, Martín-Nalda, A, Martínez, AP, Bernardo, D, Rojo, S, Fiz-López, A, Arribas, E, de la Cal-Sabater, P, Segura, T, González-Villa, E, Serrano-Heras, G, Martí-Fàbregas, J, Jiménez-Xarrié, E, de Felipe Mimbrera, A, Masjuan, J, García-Madrona, S, Domínguez-Mayoral, A, Villalonga, JM, Menéndez-Valladares, P, Chasman, DI, Sesso, HD, Manson, JE, Buring, JE, Ridker, PM, Franco, G, Davis, L, Lee, S, Priest, J, Sankaran, VG, van Heel, D, Biesecker, L, Kerchberger, VE, Baillie, JK, Pathak, Gita A., Karjalainen, Juha, Stevens, Christine, Neale, Benjamin M., Daly, Mark, Ganna, Andrea, Andrews, Shea J., Kanai, Masahiro, Cordioli, Mattia, Polimanti, Renato, Harerimana, Nadia, Pirinen, Matti, Liao, Rachel G., Chwialkowska, Karolina, Trankiem, Amy, Balaconis, Mary K., Nguyen, Huy, Solomonson, Matthew, Veerapen, Kumar, Wolford, Brooke, Roberts, Genevieve, Park, Danny, Ball, Catherine A., Coignet, Marie, McCurdy, Shannon, Knight, Spencer, Partha, Raghavendran, Rhead, Brooke, Zhang, Miao, Berkowitz, Nathan, Gaddis, Michael, Noto, Keith, Ruiz, Luong, Pavlovic, Milo, Hong, Eurie L., Rand, Kristin, Girshick, Ahna, Guturu, Harendra, Baltzell, Asher Haug, Niemi, Mari E. K., Rahmouni, Souad, Guntz, Julien, Beguin, Yve, Pigazzini, Sara, Nkambule, Lindokuhle, Georges, Michel, Moutschen, Michel, Misset, Benoit, Darcis, Gille, Guiot, Julien, Azarzar, Samira, Gofflot, Stéphanie, Claassen, Sabine, Malaise, Olivier, Huynen, Pascale, Meuris, Christelle, Thys, Marie, Jacques, Jessica, Léonard, Philippe, Frippiat, Frederic, Giot, Jean-Baptiste, Sauvage, Anne-Sophie, Frenckell, Christian Von, Belhaj, Yasmine, Lambermont, Bernard, Nakanishi, Tomoko, Morrison, David R., Mooser, Vincent, Richards, J. Brent, Butler-Laporte, Guillaume, Forgetta, Vincenzo, Li, Rui, Ghosh, Biswarup, Laurent, Laetitia, Belisle, Alexandre, Henry, Danielle, Abdullah, Tala, Adeleye, Olumide, Mamlouk, Noor, Kimchi, Nofar, Afrasiabi, Zaman, Rezk, Nardin, Vulesevic, Branka, Bouab, Meriem, Guzman, Charlotte, Petitjean, Loui, Tselios, Chri, Xue, Xiaoqing, Afilalo, Jonathan, Afilalo, Marc, Oliveira, Maureen, Brenner, Bluma, Brassard, Nathalie, Durand, Madeleine, Schurr, Erwin, Lepage, Pierre, Ragoussis, Jianni, Auld, Daniel, Chassé, Michaël, Kaufmann, Daniel E., Lathrop, G. Mark, Adra, Darin, Hayward, Caroline, Glessner, Joseph T., Shaw, Douglas M., Campbell, Archie, Morris, Marcela, Hakonarson, Hakon, Porteous, David J., Below, Jennifer, Richmond, Anne, Chang, Xiao, Polikowski, Hannah, Lauren, Petty E., Chen, Hung-Hsin, Wanying, Zhu, Fawns-Ritchie, Chloe, North, Kari, McCormick, Joseph B., Glessner, Joseph R., Gignoux, Christopher R., Wicks, Stephen J., Crooks, Kristy, Barnes, Kathleen C., Daya, Michelle, Shortt, Jonathan, Rafaels, Nichola, Chavan, Sameer, Timmers, Paul R. H. J., Wilson, James F., Tenesa, Albert, Kerr, Shona M., D’Mellow, Kenton, Shahin, Doaa, El-Sherbiny, Yasser M., von Hohenstaufen, Kathrin Aprile, Sobh, Ali, Eltoukhy, Madonna M., Nkambul, Lindokuhle, Elhadidy, Tamer A., Abd Elghafar, Mohamed S., El-Jawhari, Jehan J., Mohamed, Attia A. S., Elnagdy, Marwa H., Samir, Amr, Abdel-Aziz, Mahmoud, Khafaga, Walid T., El-Lawaty, Walaa M., Torky, Mohamed S., El-shanshory, Mohamed R., Yassen, Amr M., Hegazy, Mohamed A. F., Okasha, Kamal, Eid, Mohammed A., Moahmed, Hanteera S., Medina-Gomez, Carolina, Ikram, M. Arfan, Uitterlinden, Andre G., Mägi, Reedik, Milani, Lili, Metspalu, Andre, Laisk, Triin, Läll, Kristi, Lepamets, Maarja, Esko, Tõnu, Reimann, Ene, Naaber, Paul, Laane, Edward, Pesukova, Jaana, Peterson, Pärt, Kisand, Kai, Tabri, Jekaterina, Allos, Raili, Hensen, Kati, Starkopf, Joel, Ringmets, Inge, Tamm, Anu, Kallaste, Anne, Alavere, Helene, Metsalu, Kristjan, Puusepp, Mairo, Batini, Chiara, Tobin, Martin D., Venn, Laura D., Lee, Paul H., Shrine, Nick, Williams, Alexander T., Guyatt, Anna L., John, Catherine, Packer, Richard J., Ali, Altaf, Free, Robert C., Wang, Xueyang, Wain, Louise V., Hollox, Edward J., Bee, Catherine E., Adams, Emma L., Palotie, Aarno, Ripatti, Samuli, Ruotsalainen, Sanni, Kristiansson, Kati, Koskelainen, Sami, Perola, Marku, Donner, Kati, Kivinen, Katja, Kaunisto, Mari, Rivolta, Carlo, Bochud, Pierre-Yve, Bibert, Stéphanie, Boillat, Noémie, Nussle, Semira Gonseth, Albrich, Werner, Quinodoz, Mathieu, Kamdar, Dhryata, Suh, Noémie, Neofytos, Dionysio, Erard, Véronique, Voide, Cathy, Friolet, R., Vollenweider, P., Pagani, J. L., Oddo, M., zu Bentrup, F. Meyer, Conen, A., Clerc, O., Marchetti, O., Guillet, A., Guyat-Jacques, C., Foucras, S., Rime, M., Chassot, J., Jaquet, M., Viollet, R. Merlet, Lannepoudenx, Y., Portopena, L., Bochud, P. Y., Desgranges, F., Filippidis, P., Guéry, B., Haefliger, D., Kampouri, E. E., Manuel, O., Munting, A., Papadimitriou-Olivgeris, M., Regina, J., Rochat-Stettler, L., Suttels, V., Tadini, E., Tschopp, J., Van Singer, M., Viala, B., Boillat-Blanco, N., Brahier, T., Hügli, O., Meuwly, J. Y., Pantet, O., Gonseth Nussle, S., Bochud, M., D’Acremont, V., Estoppey Younes, S., Albrich, W. C., Suh, N., Cerny, A., O’Mahony, L., von Mering, C., Frischknecht, M., Kleger, G-R., Filipovic, M., Kahlert, C. R., Wozniak, H., Negro, T. Rochat, Pugin, J., Bouras, K., Knapp, C., Egger, T., Perret, A., Montillier, P., di Bartolomeo, C., Barda, B., de Cid, Rafael, Carreras, Anna, Moreno, Victor, Kogevinas, Manoli, Galván-Femenía, Iván, Blay, Natalia, Farré, Xavier, Sumoy, Lauro, Cortés, Beatriz, Mercader, Josep Maria, Guindo-Martinez, Marta, Torrents, David, Garcia-Aymerich, Judith, Castaño-Vinyals, Gemma, Dobaño, Carlota, Gori, Marco, Renieri, Alessandra, Mari, Francesca, Mondelli, Mario Umberto, Castelli, Francesco, Vaghi, Massimo, Rusconi, Stefano, Montagnani, Francesca, Bargagli, Elena, Franchi, Federico, Mazzei, Maria Antonietta, Cantarini, Luca, Tacconi, Danilo, Feri, Marco, Scala, Raffaele, Spargi, Genni, Nencioni, Cesira, Bandini, Maria, Caldarelli, Gian Piero, Canaccini, Anna, Ognibene, Agostino, D’Arminio Monforte, Antonella, Girardis, Massimo, Antinori, Andrea, Francisci, Daniela, Schiaroli, Elisabetta, Scotton, Pier Giorgio, Panese, Sandro, Scaggiante, Renzo, Monica, Matteo Della, Capasso, Mario, Fiorentino, Giuseppe, Castori, Marco, Aucella, Filippo, Biagio, Antonio Di, Masucci, Luca, Valente, Serafina, Mandalà, Marco, Zucchi, Patrizia, Giannattasio, Ferdinando, Coviello, Domenico A., Mussini, Cristina, Tavecchia, Luisa, Crotti, Lia, Rizzi, Marco, Rovere, Maria Teresa La, Sarzi-Braga, Simona, Bussotti, Maurizio, Ravaglia, Sabrina, Artuso, Rosangela, Perrella, Antonio, Romani, Davide, Bergomi, Paola, Catena, Emanuele, Vincenti, Antonella, Ferri, Claudio, Grassi, Davide, Pessina, Gloria, Tumbarello, Mario, Pietro, Massimo Di, Sabrina, Ravaglia, Luchi, Sauro, Furini, Simone, Dei, Simona, Benetti, Elisa, Picchiotti, Nicola, Sanarico, Maurizio, Ceri, Stefano, Pinoli, Pietro, Raimondi, Francesco, Biscarini, Filippo, Stella, Alessandra, Zguro, Kristina, Capitani, Katia, Tanfoni, Marco, Fallerini, Chiara, Daga, Sergio, Baldassarri, Margherita, Fava, Francesca, Frullanti, Elisa, Valentino, Floriana, Doddato, Gabriella, Giliberti, Annarita, Tita, Rossella, Amitrano, Sara, Bruttini, Mirella, Croci, Susanna, Meloni, Ilaria, Mencarelli, Maria Antonietta, Rizzo, Caterina Lo, Pinto, Anna Maria, Beligni, Giada, Tommasi, Andrea, Sarno, Laura Di, Palmieri, Maria, Carriero, Miriam Lucia, Alaverdian, Diana, Busani, Stefano, Bruno, Raffaele, Vecchia, Marco, Belli, Mary Ann, Mantovani, Stefania, Ludovisi, Serena, Quiros-Roldan, Eugenia, Antoni, Melania Degli, Zanella, Isabella, Siano, Matteo, Emiliozzi, Arianna, Fabbiani, Massimiliano, Rossetti, Barbara, Bergantini, Laura, D’Alessandro, Miriana, Cameli, Paolo, Bennett, David, Anedda, Federico, Marcantonio, Simona, Scolletta, Sabino, Guerrini, Susanna, Conticini, Edoardo, Frediani, Bruno, Spertilli, Chiara, Donati, Alice, Guidelli, Luca, Corridi, Marta, Croci, Leonardo, Piacentini, Paolo, Desanctis, Elena, Cappelli, Silvia, Verzuri, Agnese, Anemoli, Valentina, Pancrazzi, Alessandro, Lorubbio, Maria, Miraglia, Federica Gaia, Venturelli, Sophie, Cossarizza, Andrea, Vergori, Alessandra, Gabrieli, Arianna, Riva, Agostino, Paciosi, Francesco, Andretta, Francesca, Gatti, Francesca, Parisi, Saverio Giuseppe, Baratti, Stefano, Piscopo, Carmelo, Russo, Roberta, Andolfo, Immacolata, Iolascon, Achille, Carella, Massimo, Merla, Giuseppe, Squeo, Gabriella Maria, Raggi, Pamela, Marciano, Carmen, Perna, Rita, Bassetti, Matteo, Sanguinetti, Maurizio, Giorli, Alessia, Salerni, Lorenzo, Parravicini, Pierpaolo, Menatti, Elisabetta, Trotta, Tullio, Coiro, Gabriella, Lena, Fabio, Martinelli, Enrico, Mancarella, Sandro, Gabbi, Chiara, Maggiolo, Franco, Ripamonti, Diego, Bachetti, Tiziana, Suardi, Claudia, Parati, Gianfranco, Bottà, Giordano, Domenico, Paolo Di, Rancan, Ilaria, Bianchi, Francesco, Colombo, Riccardo, Barbieri, Chiara, Acquilini, Donatella, Andreucci, Elena, Segala, Francesco Vladimiro, Tiseo, Giusy, Falcone, Marco, Lista, Mirjam, Poscente, Monica, Vivo, Oreste De, Petrocelli, Paola, Guarnaccia, Alessandra, Baroni, Silvia, van Heel, David A., Hunt, Karen A., Trembath, Richard C., Huang, Qin Qin, Martin, Hilary C., Mason, Dan, Trivedi, Bhavi, Wright, John, Finer, Sarah, Akhtar, Shaheen, Anwar, Mohammad, Arciero, Elena, Ashraf, Samina, Breen, Gerome, Chung, Raymond, Curtis, Charles J., Chowdhury, Maharun, Colligan, Grainne, Deloukas, Pano, Durham, Ceri, Griffiths, Chri, Hurles, Matt, Hussain, Shapna, Islam, Kamrul, Khan, Ahsan, Khan, Amara, Lavery, Cath, Lee, Sang Hyuck, Lerner, Robin, MacArthur, Daniel, MacLaughlin, Bev, Martin, Hilary, Miah, Shefa, Newman, Bill, Safa, Nishat, Tahmasebi, Farah, Griffiths, Christopher J., Smith, Albert V., Boughton, Andrew P., Li, Kevin W., LeFaive, Jonathon, Annis, Aubrey, Niavarani, Ahmadreza, Aliannejad, Rasoul, Sharififard, Bahareh, Amirsavadkouhi, Ali, Naderpour, Zeinab, Tadi, Hengameh Ansari, Aleagha, Afshar Etemadi, Ahmadi, Saeideh, Moghaddam, Seyed Behrooz Mohseni, Adamsara, Alireza, Saeedi, Morteza, Abdollahi, Hamed, Hosseini, Abdolmajid, Chariyavilaskul, Pajaree, Jantarabenjakul, Watsamon, Hirankarn, Nattiya, Chamnanphon, Monpat, Suttichet, Thitima B., Shotelersuk, Vorasuk, Pongpanich, Monnat, Phokaew, Chureerat, Chetruengchai, Wanna, Putchareon, Opa, Torvorapanit, Pattama, Puthanakit, Thanyawee, Suchartlikitwong, Pintip, Nilaratanakul, Voraphoj, Sodsai, Pimpayao, Brumpton, Ben M., Hveem, Kristian, Willer, Cristen, Zhou, Wei, Rogne, Tormod, Solligard, Erik, Åsvold, Bjørn Olav, Franke, Lude, Boezen, Marike, Deelen, Patrick, Claringbould, Annique, Lopera, Esteban, Warmerdam, Robert, Vonk, Judith. M., van Blokland, Irene, Lanting, Pauline, Ori, Anil P. S., Feng, Yen-Chen Anne, Mercader, Josep, Weiss, Scott T., Karlson, Elizabeth W., Smoller, Jordan W., Murphy, Shawn N., Meigs, James B., Woolley, Ann E., Green, Robert C., Perez, Emma F., Zöllner, Sebastian, Wang, Jiongming, Beck, Andrew, Sloofman, Laura G., Ascolillo, Steven, Sebra, Robert P., Collins, Brett L., Levy, Te, Buxbaum, Joseph D., Sealfon, Stuart C., Jordan, Daniel M., Thompson, Ryan C., Gettler, Kyle, Chaudhary, Kumardeep, Belbin, Gillian M., Preuss, Michael, Hoggart, Clive, Choi, Sam, Underwood, Slayton J., Salib, Irene, Britvan, Bari, Keller, Katherine, Tang, Lara, Peruggia, Michael, Hiester, Liam L., Niblo, Kristi, Aksentijevich, Alexandra, Labkowsky, Alexander, Karp, Avromie, Zlatopolsky, Menachem, Zyndorf, Marissa, Charney, Alexander W., Beckmann, Noam D., Schadt, Eric E., Abul-Husn, Noura S., Cho, Judy H., Itan, Yuval, Kenny, Eimear E., Loos, Ruth J. F., Nadkarni, Girish N., Do, Ron, O’Reilly, Paul, Huckins, Laura M., Ferreira, Manuel A. R., Abecasis, Goncalo R., Leader, Joseph B., Cantor, Michael N., Justice, Anne E., Carey, Dave J., Chittoor, Geetha, Josyula, Navya Shilpa, Kosmicki, Jack A., Horowitz, Julie E., Baras, Ari, Gass, Matthew C., Yadav, Ashish, Mirshahi, Tooraj, Hottenga, Jouke Jan, Bartels, Meike, de geus, Eco E. J. C., Nivard, Michel M. G., Verma, Anurag, Ritchie, Marylyn D., Rader, Daniel, Li, Binglan, Verma, Shefali S., Lucas, Anastasia, Bradford, Yuki, Abedalthagafi, Malak, Alaamery, Manal, Alshareef, Abdulraheem, Sawaji, Mona, Massadeh, Salam, AlMalik, Abdulaziz, Alqahtani, Saleh, Baraka, Dona, Harthi, Fawz Al, Alsolm, Ebtehal, Safieh, Leen Abu, Alowayn, Albandary M., Alqubaishi, Fatimah, Mutairi, Amal Al, Mangul, Serghei, Almutairi, Mansour, Aljawini, Nora, Albesher, Nour, Arabi, Yaseen M., Mahmoud, Ebrahim S., Khattab, Amin K., Halawani, Roaa T., Alahmadey, Ziab Z., Albakri, Jehad K., Felemban, Walaa A., Suliman, Bandar A., Hasanato, Rana, Al-Awdah, Laila, Alghamdi, Jahad, AlZahrani, Deema, AlJohani, Sameera, Al-Afghani, Hani, AlDhawi, Nouf, AlBardis, Hadeel, Alkwai, Sarah, Alswailm, Moneera, Almalki, Faisal, Albeladi, Maha, Almohammed, Iman, Barhoush, Eman, Albader, Anoud, Alotaibi, Sara, Alghamdi, Bader, Jung, Junghyun, fawzy, Mohammad S., Alrashed, May, Zeberg, Hugo, Nkambul, Lindo, Frithiof, Robert, Hultström, Michael, Lipcsey, Miklo, Tardif, Nicola, Rooyackers, Olav, Grip, Jonathan, Maricic, Tomislav, Helgeland, Øyvind, Magnus, Per, Trogstad, Lill-Iren S., Lee, Yunsung, Harris, Jennifer R., Mangino, Massimo, Spector, Tim D., Emma, Duncan, Moutsianas, Louka, Caulfield, Mark J., Scott, Richard H., Kousathanas, Athanasio, Pasko, Dorota, Walker, Susan, Stuckey, Alex, Odhams, Christopher A., Rhodes, Daniel, Fowler, Tom, Rendon, Augusto, Chan, Georgia, Arumugam, Prabhu, Karczewski, Konrad J., Martin, Alicia R., Wilson, Daniel J., Spencer, Chris C. A., Crook, Derrick W., Wyllie, David H., O’Connell, Anne Marie, Atkinson, Elizabeth G., Tsuo, Kristin, Baya, Nikola, Turley, Patrick, Gupta, Rahul, Walters, Raymond K., Palmer, Duncan S., Sarma, Gopal, Cheng, Nathan, Lu, Wenhan, Churchhouse, Claire, Goldstein, Jacqueline I., King, Daniel, Seed, Cotton, Daly, Mark J., Finucane, Hilary, Bryant, Sam, Satterstrom, F. Kyle, Band, Gavin, Earle, Sarah G., Lin, Shang-Kuan, Arning, Nicola, Koelling, Nil, Armstrong, Jacob, Rudkin, Justine K., Callier, Shawneequa, Cusick, Caroline, Soranzo, Nicole, Zhao, Jing Hua, Danesh, John, Angelantonio, Emanuele Di, Butterworth, Adam S., Sun, Yan V., Huffman, Jennifer E., Cho, Kelly, O’Donnell, Christopher J., Tsao, Phil, Gaziano, J. Michael, Peloso, Gina, Ho, Yuk-Lam, Smieszek, Sandra P., Polymeropoulos, Christo, Polymeropoulos, Vasilio, Polymeropoulos, Mihael H., Przychodzen, Bartlomiej P., Fernandez-Cadenas, Israel, Planas, Anna M., Perez-Tur, Jordi, Llucià-Carol, Laia, Cullell, Natalia, Muiño, Elena, Cárcel-Márquez, Jara, DeDiego, Marta L., Iglesias, Lara Lloret, Soriano, Alex, Rico, Veronica, Agüero, Daiana, Bedini, Josep L., Lozano, Francisco, Domingo, Carlo, Robles, Veronica, Ruiz-Jaén, Francisca, Márquez, Leonardo, Gomez, Juan, Coto, Eliecer, Albaiceta, Guillermo M., García-Clemente, Marta, Dalmau, David, Arranz, Maria J., Dietl, Beatriz, Serra-Llovich, Alex, Soler, Pere, Colobrán, Roger, Martín-Nalda, Andrea, Martínez, Alba Parra, Bernardo, David, Rojo, Silvia, Fiz-López, Aida, Arribas, Elisa, de la Cal-Sabater, Paloma, Segura, Tomá, González-Villa, Esther, Serrano-Heras, Gemma, Martí-Fàbregas, Joan, Jiménez-Xarrié, Elena, de Felipe Mimbrera, Alicia, Masjuan, Jaime, García-Madrona, Sebastian, Domínguez-Mayoral, Anna, Villalonga, Joan Montaner, Menéndez-Valladares, Paloma, Chasman, Daniel I., Sesso, Howard D., Manson, JoAnn E., Buring, Julie E., Ridker, Paul M., Franco, Giulianini, Davis, Lea, Lee, Sulggi, Priest, Jame, Sankaran, Vijay G., van Heel, David, Biesecker, Le, Kerchberger, V. Eric, Baillie, J. Kenneth, APH - Personalized Medicine, APH - Health Behaviors & Chronic Diseases, Biological Psychology, APH - Mental Health, AMS - Sports, AMS - Ageing & Vitality, APH - Methodology, Mccurdy, Shannon, Mccormick, Joseph B., Macarthur, Daniel, Maclaughlin, Bev, Lefaive, Jonathon, Almalik, Abdulaziz, Alzahrani, Deema, Aljohani, Sameera, Aldhawi, Nouf, Albardis, Hadeel, Fawzy, Mohammad S., Dediego, Marta L., Stem Cell Aging Leukemia and Lymphoma (SALL), Groningen Institute for Gastro Intestinal Genetics and Immunology (3GI), Life Course Epidemiology (LCE), Groningen Research Institute for Asthma and COPD (GRIAC), University of Zurich, COVID-19 Host Genetics Initiative, Barcelona Supercomputing Center, COVID-19 Genetics Initiative, including authors, Institute for Molecular Medicine Finland, and Data Science Genetic Epidemiology Lab
Subjects: Informàtica::Aplicacions de la informàtica::Bioinformàtica [Àrees temàtiques de la UPC], Quantitative Trait Loci, MUC5B PROMOTER POLYMORPHISM, Genome-wide association studies, COVID-19 (Malaltia), UFSP13-7 Evolution in Action: From Genomes to Ecosystems, COVID-19 (Disease), Settore BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA, SDG 3 - Good Health and Well-being, Humans, genetics, Genetic variation, Genomes, Medicinsk genetik, 1000 Multidisciplinary, Multidisciplinary, Chromosome Mapping, COVID-19, Human Genetics, 10124 Institute of Molecular Life Sciences, covid-19, 3121 General medicine, internal medicine and other clinical medicine, 570 Life sciences, biology, Medical Genetics
Abstract: Matters arising from: Mapping the human genetic architecture of COVID-19 Original Article published on 08 July 2021 https://www.nature.com/articles/s41586-021-03767-x The COVID-19 pandemic continues to pose a major public health threat, especially in countries with low vaccination rates. To better understand the biological underpinnings of SARS-CoV-2 infection and COVID-19 severity, we formed the COVID-19 Host Genetics Initiative1. Here we present a genome-wide association study meta-analysis of up to 125,584 cases and over 2.5 million control individuals across 60 studies from 25 countries, adding 11 genome-wide significant loci compared with those previously identified2. Genes at new loci, including SFTPD, MUC5B and ACE2, reveal compelling insights regarding disease susceptibility and severity. Here we present meta-analyses bringing together 60 studies from 25 countries (Fig. 1 and Supplementary Table 1) for three COVID-19-related phenotypes: (1) individuals critically ill with COVID-19 on the basis of requiring respiratory support in hospital or who died as a consequence of the disease (9,376 cases, of which 3,197 are new in this data release, and 1,776,645 control individuals); (2) individuals with moderate or severe COVID-19 defined as those hospitalized due to symptoms associated with the infection (25,027 cases, 11,386 new and 2,836,272 control individuals); and (3) all cases with reported SARS-CoV-2 infection regardless of symptoms (125,584 cases, 76,022 new and 2,575,347 control individuals). Most studies have reported results before the roll out of the COVID-19 vaccination campaign. An overview of the study design is provided in Supplementary Fig. 1. We found a total of 23 genome-wide significant loci (P
Published: 2022

36. High-resolution genetic map construction and QTL analysis of important fiber traits in kenaf using RAD-seq

Author: Luan Mingbao, Chen Anguo, Li Hui, Chang Li, Wu Yena, Pan Gen, Jiang Hui, Huang Siqi, and Tang Huijuan
Subjects: Qtl analysis, High resolution, Computational biology, Fiber, Plant Science, Biology, biology.organism_classification, Kenaf
Abstract: Quantitative trait locus (QTL) mapping is a useful method for revealing the mechanism of complex genetic traits and identifying new genomic information to accelerate crop improvement. In the present study, 154 F2:3 strains and their parents were used for restriction site-associated DNA sequencing, single-nucleotide polymorphism (SNP) identification, and genetic map construction. After filtering based on stringent filtering standards, 297.5 Gb of clean data were obtained. Further, 5,191 polymorphic SNP markers were identified from each sample, of which 1,997 polymorphic SNP markers were successfully mapped onto 18 different linkage groups. Six QTLs (QPH, QFBW, QDBW, QFW, QFT, and QFC) were identified based on the genetic map using the multiple QTL mapping (MQM) method, which were then assigned to three linkage groups, LG16, LG8, and LG3. QPH, QFBW, QDBW, and QFW were related to fiber yield, while QFT and QFC were related to fiber quality. This is the first study of its kind to map QTL of fiber yield and fiber quality, which will facilitate further understanding of the molecular genetic basis of these traits. However, there are limitations regarding the utilization of this map because several large gaps remain in some linkage groups. Therefore, additional markers need to be developed to further narrow these regions.
Published: 2023

37. Effects of BDE-209 exposure on growth performance, intestinal digestive enzymes, and intestinal microbiome in common carp (Cyprinus carpio L.)

Author: Meiru Kang, Xulu Chang, Junchang Feng, Xianfeng Wang, and Jianxin Zhang
Subjects: Pollutant, chemistry.chemical_classification, 0303 health sciences, Ecology, Zoology, 04 agricultural and veterinary sciences, Aquatic Science, Biology, biology.organism_classification, medicine.disease_cause, Cyprinus, 03 medical and health sciences, Common carp, Immune system, Enzyme, chemistry, Intestinal Microbiome, 040102 fisheries, medicine, 0401 agriculture, forestry, and fisheries, Endocrine system, Ecology, Evolution, Behavior and Systematics, Oxidative stress, 030304 developmental biology
Abstract: Polybrominated diphenyl ether has become a widespread environmental pollutant in aquatic environments. BDE-209, a prominent PBDEs found in the environment and wildlife, has been proven to cause oxidative stress, immune damage and endocrine system disorders in fish. However, the effects of BDE-209 on the intestinal health of fish remain unclear. This research aimed to determine the effects of BDE-209 on the growth performance, digestive enzymes activities and intestinal microbiota of common carp. After 60-day exposure, the growth performance of common carp were remarkably decreased (P 0.05). Overall, exposure to BDE-209 could alter the intestinal microbiota of common carp, which may affect their growth and threaten their health.
Published: 2023

38. Clinical characteristics and outcomes of non-cystic fibrosis patients with Burkholderia cepacia complex bacteremia at a medical center in Taiwan

Author: Jann-Tay Wang, Wang-Huei Sheng, Yu-Chung Chuang, and Tien-Hao Chang
Subjects: Adult, Microbiology (medical), medicine.medical_specialty, Cystic Fibrosis, Taiwan, Bacteremia, Levofloxacin, Burkholderia cepacia, Logistic regression, Trimethoprim, Internal medicine, Humans, Immunology and Allergy, Medicine, Risk factor, Aged, Retrospective Studies, General Immunology and Microbiology, biology, business.industry, Burkholderia cepacia complex, Burkholderia Infections, Retrospective cohort study, General Medicine, Odds ratio, biology.organism_classification, medicine.disease, Fibrosis, Hospitals, Anti-Bacterial Agents, Regimen, Infectious Diseases, business, medicine.drug
Abstract: BACKGROUND Burkholderia cepacia complex (BCC) represents a group of multidrug-resistant gram-negative bacteria that cause infections among immunocompromised hosts. Bacteremia occurs in patients who are chronically ill and is associated with substantial morbidity and mortality. The aim of this study was to investigate the clinical characteristics and outcomes of BCC bacteremic patients without cystic fibrosis. METHODS We conducted a retrospective study at the National Taiwan University Hospital. Adults with BCC bacteremia from January 2015 to May 2019 were enrolled. The primary outcome was 14-day mortality. Multivariable logistic regression was performed for outcome analysis. RESULTS One-hundred and ninety-five patients were analyzed and their mean age was 67 years. Over 95% of the BCC isolates were susceptible to trimethoprim/sulfomethoxazole (TMP/SXT). Levofloxacin resistance rates were high, with only 25.1% of isolates being susceptible. Pairwise comparisons were made between different definitive regimens including meropenem-monotherapy, ceftazidime-monotherapy, levofloxacin-monotherapy, TMP/SXT-monotherapy, tigecycline-monotherapy as well as combination versus monotherapy. No regimen was significantly associated with survival in our study. Multivariable logistic regression showed that the Pitt bacteremia score (adjust odds ratio [aOR],1.46; 95% confidence interval [CI],1.19-1.79; p
Published: 2022

39. CaO and Graphene Oxide Enhances Drought Stress from Callus Tissues of Medicago Sativa L. Cultivars

Author: YAZICILAR, Büşra, BEZİRGANOGLU, İsmail, CHANG, Yen Ling, and NADAR, Muthukumar
Subjects: Alfalfa, nanoparticules, mannitol, in vitro culture, Biology, Biyoloji
Abstract: Drought stress can be described as multidimensional stress factors affecting plants’ growth, development, and productivity. In order to reduce the adverse impact of drought stress, a plethora of attempts have been employed. Among those attempts, nano-engineered particles have gained a remarkable attention. Of the relevant particles, calcium oxide (CaO) and graphene oxide (GO) have been well-documented to positively regulate and mediate the plant growth system through shifting physiological biochemical and molecular aspects of the plant. The solo impacts of the nanoparticles are well-known but their interactions were not assayed for Medicago sativa L. cultivars. For that reason, the present study investigates the impact of CaO NPs and GO on the response and regulation of the defensive mechanism in alfalfa (Medicago sativa L.) callus in drought stress-suffered cultivars. The activation of CaO-GO can be induced with mannitol in the callus of alfalfa cultivars. Dry and fresh weight values were determined in callus samples. There were significant differences between cultivars and concentration. In terms of MDA, H2O2, proline content, it was observed that the Ca2+ NPs application was important, and it showed a strong link with the resistance degree of cultivars. Erzurum cultivar was observed for better proline content with 1.5 ppm GO. MDA activities demonstrated an increasing trend concerning concentrations of mannitol and nanoparticles. The MDA highest activity was observed with 1/2 ppm CaO+0.5/1.5 ppm GO (0.1849 mg/g FW) in the Erzurum. However, the Erzurum cultivar responded with better H2O2 content with 100 mM mannitol +0.5 ppm (0.1017 mg/g FW). This result has presented, under in vitro conditions, that the supplementation of CaO and GO can importantly reduce the negative impacts of drought stress on alfalfa callus; additionally, it has been seen that the dosages of nanoparticle and mannitol are also important.
Published: 2022

40. Association of capsular polysaccharide locus 2 with prognosis of Acinetobacter baumannii bacteraemia

Author: Shan-Chwen Chang, Yee-Chun Chen, Chia-Jui Yang, Wang-Huei Sheng, Yu-Chung Chuang, and Jia-Ling Yang
Subjects: medicine.medical_specialty, Imipenem, Epidemiology, Immunology, Tigecycline, Infectious and parasitic diseases, RC109-216, Microbiology, Virology, White blood cell, Internal medicine, Drug Discovery, medicine, capsule locus 2, biology, business.industry, Hazard ratio, General Medicine, Sulbactam, biology.organism_classification, medicine.disease, bacterial infections and mycoses, Confidence interval, capsular polysaccharide, QR1-502, Acinetobacter baumannii, virulence, Pneumonia, Infectious Diseases, medicine.anatomical_structure, outcome, Parasitology, business, acinetobacter baumannii, medicine.drug
Abstract: Acinetobacter baumannii causes healthcare-associated infections worldwide. Capsular polysaccharide (CPS) is shown an important virulence factor of A. baumannii both in vitro and in vivo. Capsule locus 2 (KL2) for CPS is the most common KL type and is associated with carbapenem resistance. It is unclear whether KL2 is related to the clinical outcome of invasive A. baumannii infection. Here we had followed patients with A. baumannii bacteraemia prospectively between 2009 and 2014. One-third of the unduplicated blood isolates were randomly selected each year for microbiological and clinical studies. The KL2 gene cluster was identified using polymerase chain reaction. A total of 148 patients were enrolled randomly. Eighteen isolates (12.2%) carried KL2, and 130 isolates (87.8%) didn't. Compared with non-KL2 isolates, KL2 isolates had significantly higher resistance to imipenem, sulbactam, and tigecycline. Compared with the non-KL group, in the KL2 group, the hospital stay before development of bacteraemia was longer (P
Published: 2022

41. Genome-wide analysis of 53,400 people with irritable bowel syndrome highlights shared genetic pathways with mood and anxiety disorders

Author: Eijsbouts, Chris, Zheng, Tenghao, Kennedy, Nicholas A., Bonfiglio, Ferdinando, Anderson, Carl A., Moutsianas, Loukas, Holliday, Joanne, Shi, Jingchunzi, Shringarpure, Suyash, Voda, Alexandru-Ioan, Farrugia, Gianrico, Franke, Andre, H��benthal, Matthias, Abecasis, Gon��alo, Zawistowski, Matthew, Skogholt, Anne Heidi, Ness-Jensen, Eivind, Hveem, Kristian, Esko, T��nu, Teder-Laving, Maris, Zhernakova, Alexandra, Camilleri, Michael, Boeckxstaens, Guy, Whorwell, Peter J., Spiller, Robin, McVean, Gil, D��Amato, Mauro, Jostins, Luke, Parkes, Miles, Agee, Michelle, Aslibekyan, Stella, Auton, Adam, Bell, Robert K., Bryc, Katarzyna, Clark, Sarah K., Elson, Sarah L., Fletez-Brant, Kipper, Fontanillas, Pierre, Furlotte, Nicholas A., Gandhi, Pooja M., Heilbron, Karl, Hicks, Barry, Hinds, David A., Huber, Karen E., Jewett, Ethan M., Jiang, Yunxuan, Kleinman, Aaron, Lin, Keng-Han, Litterman, Nadia K., Luff, Marie K., McCreight, Jey C., McIntyre, Matthew H., McManus, Kimberly F., Mountain, Joanna L., Mozaffari, Sahar V., Nandakumar, Priyanka, Noblin, Elizabeth S., Northover, Carrie A. M., O��Connell, Jared, Petrakovitz, Aaron A., Pitts, Steven J., Poznik, G. David, Sathirapongsasuti, J. Fah, Shastri, Anjali J., Shelton, Janie F., Tian, Chao, Tung, Joyce Y., Tunney, Robert J., Vacic, Vladimir, Wang, Xin, Zare, Amir S., Kashyap, Purna, Chang, Lin, Mayer, Emeran, Heitkemper, Margaret, Sayuk, Gregory S., Ringel-Kulka, Tamar, Ringel, Yehuda, Chey, William D., Eswaran, Shanti, Merchant, Juanita L., Shulman, Robert J., Bujanda, Luis, Garcia-Etxebarria, Koldo, Dlugosz, Aldona, Lindberg, Greger, Schmidt, Peter T., Karling, Pontus, Ohlsson, Bodil, Walter, Susanna, Faresj��, ��shild O., Simren, Magnus, Halfvarson, Jonas, Portincasa, Piero, Barbara, Giovanni, Usai-Satta, Paolo, Neri, Matteo, Nardone, Gerardo, Cuomo, Rosario, Galeazzi, Francesca, Bellini, Massimo, Latiano, Anna, Houghton, Lesley, Jonkers, Daisy, Kurilshikov, Alexander, Weersma, Rinse K., Netea, Mihai, Tesarz, Jonas, Gauss, Annika, Goebel-Stengel, Miriam, Andresen, Viola, Frieling, Thomas, Pehl, Christian, Schaefert, Rainer, Niesler, Beate, Lieb, Wolfgang, Hanevik, Kurt, Langeland, Nina, Wensaas, Knut-Arne, Litleskare, Sverre, Gabrielsen, Maiken E., Thomas, Laurent, Thijs, Vincent, Lemmens, Robin, Van Oudenhove, Lukas, Wouters, Mira, Eijsbouts C., Zheng T., Kennedy N.A., Bonfiglio F., Anderson C.A., Moutsianas L., Holliday J., Shi J., Shringarpure S., Agee M., Aslibekyan S., Auton A., Bell R.K., Bryc K., Clark S.K., Elson S.L., Fletez-Brant K., Fontanillas P., Furlotte N.A., Gandhi P.M., Heilbron K., Hicks B., Hinds D.A., Huber K.E., Jewett E.M., Jiang Y., Kleinman A., Lin K.-H., Litterman N.K., Luff M.K., McCreight J.C., McIntyre M.H., McManus K.F., Mountain J.L., Mozaffari S.V., Nandakumar P., Noblin E.S., Northover C.A.M., O'Connell J., Petrakovitz A.A., Pitts S.J., Poznik G.D., Sathirapongsasuti J.F., Shastri A.J., Shelton J.F., Tian C., Tung J.Y., Tunney R.J., Vacic V., Wang X., Zare A.S., Voda A.-I., Kashyap P., Chang L., Mayer E., Heitkemper M., Sayuk G.S., Ringel-Kulka T., Ringel Y., Chey W.D., Eswaran S., Merchant J.L., Shulman R.J., Bujanda L., Garcia-Etxebarria K., Dlugosz A., Lindberg G., Schmidt P.T., Karling P., Ohlsson B., Walter S., Faresjo A.O., Simren M., Halfvarson J., Portincasa P., Barbara G., Usai-Satta P., Neri M., Nardone G., Cuomo R., Galeazzi F., Bellini M., Latiano A., Houghton L., Jonkers D., Kurilshikov A., Weersma R.K., Netea M., Tesarz J., Gauss A., Goebel-Stengel M., Andresen V., Frieling T., Pehl C., Schaefert R., Niesler B., Lieb W., Hanevik K., Langeland N., Wensaas K.-A., Litleskare S., Gabrielsen M.E., Thomas L., Thijs V., Lemmens R., Van Oudenhove L., Wouters M., Farrugia G., Franke A., Hubenthal M., Abecasis G., Zawistowski M., Skogholt A.H., Ness-Jensen E., Hveem K., Esko T., Teder-Laving M., Zhernakova A., Camilleri M., Boeckxstaens G., Whorwell P.J., Spiller R., McVean G., D'Amato M., Jostins L., Parkes M., Eijsbouts, Chris [0000-0001-5179-0653], Anderson, Carl A. [0000-0003-1719-7009], Moutsianas, Loukas [0000-0001-5453-345X], Holliday, Joanne [0000-0003-4568-7320], Shringarpure, Suyash [0000-0001-6464-2668], Voda, Alexandru-Ioan [0000-0003-2974-6992], Farrugia, Gianrico [0000-0003-3473-5235], Hübenthal, Matthias [0000-0002-5956-3006], Abecasis, Gonçalo [0000-0003-1509-1825], Zawistowski, Matthew [0000-0002-3005-083X], Ness-Jensen, Eivind [0000-0001-6005-0729], Teder-Laving, Maris [0000-0002-5872-1850], Camilleri, Michael [0000-0001-6472-7514], Whorwell, Peter J. [0000-0002-5220-8474], Spiller, Robin [0000-0001-6371-4500], McVean, Gil [0000-0002-5012-4162], D’Amato, Mauro [0000-0003-2743-5197], Jostins, Luke [0000-0002-2475-3969], Parkes, Miles [0000-0002-6467-0631], Apollo - University of Cambridge Repository, Groningen Institute for Gastro Intestinal Genetics and Immunology (3GI), Translational Immunology Groningen (TRIGR), Eijsbouts, C., Zheng, T., Kennedy, N. A., Bonfiglio, F., Anderson, C. A., Moutsianas, L., Holliday, J., Shi, J., Shringarpure, S., Agee, M., Aslibekyan, S., Auton, A., Bell, R. K., Bryc, K., Clark, S. K., Elson, S. L., Fletez-Brant, K., Fontanillas, P., Furlotte, N. A., Gandhi, P. M., Heilbron, K., Hicks, B., Hinds, D. A., Huber, K. E., Jewett, E. M., Jiang, Y., Kleinman, A., Lin, K. -H., Litterman, N. K., Luff, M. K., Mccreight, J. C., Mcintyre, M. H., Mcmanus, K. F., Mountain, J. L., Mozaffari, S. V., Nandakumar, P., Noblin, E. S., Northover, C. A. M., O'Connell, J., Petrakovitz, A. A., Pitts, S. J., Poznik, G. D., Sathirapongsasuti, J. F., Shastri, A. J., Shelton, J. F., Tian, C., Tung, J. Y., Tunney, R. J., Vacic, V., Wang, X., Zare, A. S., Voda, A. -I., Kashyap, P., Chang, L., Mayer, E., Heitkemper, M., Sayuk, G. S., Ringel-Kulka, T., Ringel, Y., Chey, W. D., Eswaran, S., Merchant, J. L., Shulman, R. J., Bujanda, L., Garcia-Etxebarria, K., Dlugosz, A., Lindberg, G., Schmidt, P. T., Karling, P., Ohlsson, B., Walter, S., Faresjo, A. O., Simren, M., Halfvarson, J., Portincasa, P., Barbara, G., Usai-Satta, P., Neri, M., Nardone, G., Cuomo, R., Galeazzi, F., Bellini, M., Latiano, A., Houghton, L., Jonkers, D., Kurilshikov, A., Weersma, R. K., Netea, M., Tesarz, J., Gauss, A., Goebel-Stengel, M., Andresen, V., Frieling, T., Pehl, C., Schaefert, R., Niesler, B., Lieb, W., Hanevik, K., Langeland, N., Wensaas, K. -A., Litleskare, S., Gabrielsen, M. E., Thomas, L., Thijs, V., Lemmens, R., Van Oudenhove, L., Wouters, M., Farrugia, G., Franke, A., Hubenthal, M., Abecasis, G., Zawistowski, M., Skogholt, A. H., Ness-Jensen, E., Hveem, K., Esko, T., Teder-Laving, M., Zhernakova, A., Camilleri, M., Boeckxstaens, G., Whorwell, P. J., Spiller, R., Mcvean, G., D'Amato, M., Jostins, L., and Parkes, M.
Subjects: Male, Molecular Chaperone, Mood Disorder, 631/208/205/2138, Biology, 692/699/1503/1502/2071, Bioinformatics, Polymorphism, Single Nucleotide, Genetic pathways, 38/43, Irritable Bowel Syndrome, Cytoskeletal Protein, Genetics, medicine, Genetic predisposition, Aged, Anxiety Disorders, CD56 Antigen, Cell Adhesion Molecules, Cytoskeletal Proteins, Female, Genetic Predisposition to Disease, Genome-Wide Association Study, Guanine Nucleotide Exchange Factors, Homeodomain Proteins, Humans, Middle Aged, Molecular Chaperones, Mood Disorders, United Kingdom, Polymorphism, 692/699/476, Irritable bowel syndrome, Depression (differential diagnoses), article, Homeodomain Protein, Single Nucleotide, Guanine Nucleotide Exchange Factor, medicine.disease, Neuroticism, Biobank, Mood, Cell Adhesion Molecule, Anxiety, medicine.symptom, Anxiety Disorder, Human
Abstract: Funder: Kennedy Trust Rheumatology Research Prize Studentship, Funder: DFG Cluster of Excellence ��Precision Medicine in Chronic In-flammation�� (PMI; ID: EXC2167), Funder: EC | EC Seventh Framework Programm | FP7 Ideas: European Research Council (FP7-IDEAS-ERC - Specific Programme: ��Ideas�� Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013)); doi: https://doi.org/10.13039/100011199; Grant(s): 715772, Funder: NWO-VIDI grant 016.178.056, the Netherlands Heart Foundation CVON grant 2018-27, and NWO Gravitation grant ExposomeNL, Funder: Li Ka Shing Foundation (Li Ka Shing Foundation Limited); doi: https://doi.org/10.13039/100007421, Irritable bowel syndrome (IBS) results from disordered brain��gut interactions. Identifying susceptibility genes could highlight the underlying pathophysiological mechanisms. We designed a digestive health questionnaire for UK Biobank and combined identified cases with IBS with independent cohorts. We conducted a genome-wide association study with 53,400 cases and 433,201 controls and replicated significant associations in a 23andMe panel (205,252 cases and 1,384,055 controls). Our study identified and confirmed six genetic susceptibility loci for IBS. Implicated genes included NCAM1, CADM2, PHF2/FAM120A, DOCK9, CKAP2/TPTE2P3 and BAG6. The first four are associated with mood and anxiety disorders, expressed in the nervous system, or both. Mirroring this, we also found strong genome-wide correlation between the risk of IBS and anxiety, neuroticism and depression (rg > 0.5). Additional analyses suggested this arises due to shared pathogenic pathways rather than, for example, anxiety causing abdominal symptoms. Implicated mechanisms require further exploration to help understand the altered brain��gut interactions underlying IBS.
Published: 2021

42. An area-efficient ultra-low-power time-domain feature extractor for edge keyword spotting

Author: Chen, Qinyu, Chang, Y, Kim, Kwantae, Gao, Chang, Liu, Shih-Chii, and University of Zurich
Subjects: 570 Life sciences, biology, 10194 Institute of Neuroinformatics
Published: 2023

43. Reproduction of Epigrapsus notatus (Brachyura: Gecarcinidae) in Taiwan

Author: Liu, Hung-Chang and Jeng, Ming-Shiou
Published: 2005

44. State of the Field: Ethylene Biology. More than a Gas

Author: Chang, Caren and Bleecker, Anthony B.
Published: 2004

45. Anti-Colonialist Antinomies in a Biology Lesson: A Sonata-Form Case Study of Cultural Conflict in a Science Classroom

Author: Chang, Paokong John and Rosiek, Jerry
Published: 2003

46. 'Nasal lining rotation flap with triangular fossa composite graft, an effective method for managing the multiply-revised Asian short nose'

Author: Victor Bong-Hang Shyu, Yen-Chang Hsiao, Cheng-I Yen, and Frank Chun-Shin Chang
Subjects: Surgical results, medicine.medical_specialty, Rotation flap, Fossa, biology, business.industry, medicine.medical_treatment, Nose Neoplasms, Intervention group, Nose, Rhinoplasty, biology.organism_classification, Surgical Flaps, Surgery, Plastic surgery, Asian People, Short nose, Nose Diseases, medicine, Humans, Composite graft, business
Abstract: Postoperative short nose is one of the most difficult problems encountered in plastic surgery. We propose a technique of lining rotation flaps combined with composite chondrocutaneous auricular graft from the triangular fossa to reconstruct the lining defect and improve surgical results.Twenty patients were operated on between 2016 and 2019 for postoperative short nose. Lining rotation flaps were used to supply missing medial mucosal lining with creation of a raw surface at the lateral lining in 10 patients (intervention group). A total of 17 composite chondrocutaneous grafts taken from the triangular fossa of the ears were used to resurface the defect. The other 10 patients received lining management using conventional techniques (control group).There was a near-complete take of 13/17 composite grafts (76.5%), with four partial losses that healed uneventfully. Anthropometric analysis of preop and postop profile photos showed statistically significant decreased nasolabial angle in both groups. The amount of derotation achieved was significantly increased (p 0.05) with our proposed method (Intervention group: 12.3 ± 9.3° vs. control group: 6.55 ± 4.5°). Donor sites healed uneventfully. Both groups of patients were satisfied with their results based on Rhinoplasty Outcomes Evaluation questionnaire.The lining rotation flap with triangular fossa composite graft is a safe and effective method for management of the postoperative short nose in Asians.
Published: 2022

47. Protein conformation and electric attraction adsorption mechanisms on anodized magnesium alloy by molecular dynamics simulations

Author: Rong-Chang Zeng, Li Wang, Shaokang Guan, Hong-Yan Wang, Xiaobo Chen, Zhao-Qi Zhang, and Cun-Guo Lin
Subjects: 010302 applied physics, Materials science, biology, Hydrogen bond, Metals and Alloys, Substrate (chemistry), 02 engineering and technology, engineering.material, 021001 nanoscience & nanotechnology, 01 natural sciences, Molecular dynamics, Adsorption, Coating, Chemical engineering, Mechanics of Materials, 0103 physical sciences, engineering, biology.protein, Molecule, Bovine serum albumin, 0210 nano-technology, Protein adsorption
Abstract: Protein adsorption preferentially occurs and significantly affects the physicochemical reactions once the biodegradable magnesium alloys as bone replacements have been implanted. To date, interactions mechanisms between Mg implants and proteins remain unclear at a molecular level. Thereby, a combination of molecular dynamic (MD) simulations and experimental exploration is used to investigate the adsorption behavior and conformational change of bovine serum albumin (BSA), a representative protein of blood plasma, upon the surface of micro-arc oxidation (MAO) coated Mg alloy AZ31. The influences of absorbed proteins on the cytocompatibility of MAO coating are evaluated by virtue of cytotoxicity assay. Results indicate that the negatively charged O atoms (BSA) exhibit strong interaction with Mg2+ ions of Mg(OH)2, revealing that BSA molecules are ionically adsorbed on the AZ31 surface. Interestingly, MD simulation reveals that MAO coating demonstrates superior ability to capture BSA molecules during the process of adsorption owing to strong electric attraction between the negatively charged O atoms in BSA molecules with Mg atoms of MgO in MAO coating. Moreover, the α-helix part of absorbed BSA molecules on AZ31 substrate and MAO coating markedly decreases with an increase in β-sheet, β-turn and unordered contents, which is attributed to the reduction in the number of hydrogen bonds in BSA molecules. Furthermore, the adsorbed BSA molecules improve the cytocompatibility of MAO coating since the positively charged -NH3+ group and β-sheet content of absorbed BSA molecules mediate the cell adhesion by interacting with the negatively charged cell membrane.
Published: 2022

48. Current progress in application of 1-Methylcyclopropene to improve postharvest quality of cut flowers

Author: Swum Yi Kyu, Aung Htay Naing, In-Kyu Kang, Nay Myo Win, and Chang Kil Kim
Subjects: Gynoecium, Ethylene, Ecology, Renewable Energy, Sustainability and the Environment, media_common.quotation_subject, fungi, Longevity, food and beverages, Plant Science, Cut flowers, Biology, 1-Methylcyclopropene, Biochemistry, Genetics and Molecular Biology (miscellaneous), Horticulture, chemistry.chemical_compound, Abscission, chemistry, Postharvest, Petal, Ecology, Evolution, Behavior and Systematics, media_common
Abstract: Floral senescence or petal abscission that determine cut flower quality and longevity are primarily caused by the regulation of endogenous ethylene and perception of endogenous and exogenous ethylene in floral organs (such as the petals and gynoecium), in ethylene-sensitive species. Several attempts have been made to prolong flower quality and longevity using silver ions and other chemicals that inhibit ethylene production and perception in floral organs. Among the available compounds, 1-methylcyclopropene (1-MCP) has been increasingly used as ethylene-action/perception inhibitor because of its ability to inhibit ethylene action/perception and it is environmentally safe. Although 1-MCP has been used in several cut flower crops for about 25 years, there has been no review yet that critically emphasizes the benefits or difficulties of using this compound for the prolongation of cut flower quality and longevity. Here, we outline the role of 1-MCP in inhibiting ethylene action/perception and petal senescence in cut flowers through the blocking of receptor binding sites, and we discuss how various factors affecting efficacy of 1-MCP (such as concentration, treatment time and temperature, genotype, and flower stage) are involved in the achievement of flower longevity. Moreover, we highlight the advantages of applying nonvolatile and liquid 1-MCP formulation types, as opposed to using the conventional 1-MCP treatment (powder formulation type). We expect that this review will provide useful information for the future utilization of 1-MCP for the maintenance of cut flower longevity.
Published: 2022

49. An updated checklist of Collembola in Taiwan, with DNA barcoding of Papirioides jacobsoni Folsom, 1924 (Symphypleona, Dicyrtomidae)

Author: Hsin-Ju Cheng, Frans Janssens, and Chih-Han Chang
Subjects: Papirioides, Dicyrtomidae, Arthropoda, Appendiciphora, Hexapoda, Biodiversity, Sminthuriformia, Biota, Poduromorpha, Ptenothricinae, Dicyrtomoidea, springtail, Animalia, Collembola, Symphypleona, Papirioides jacobsoni, Animal Science and Zoology, Entomobryomorpha, Biology, Ecology, Evolution, Behavior and Systematics
Abstract: From urban green space to pristine forest, Collembola is one of the most numerous and species-rich members of the soil fauna around the world. However, due to lack of taxonomic expertise and research, its diversity is poorly understood, especially in tropical and subtropical regions. Collembola biodiversity studies in Taiwan have not seen much progress since 1981, when Hsin Chi reviewed 26 species belonging to 20 genera and eight families. Additionally, reports of new records in Taiwan in the last 40 years are scattered amongst several publications and not easily accessible to most end-users. Thus, a concise summary of related research is urgently needed. In this study, we updated the checklist of Collembola in Taiwan, based on published papers as well as images recorded in 2020–2022. We concluded that 58 species of Collembola belonging to 31 genera and 12 families have been reported in Taiwan, including 13 newly-recorded species. This species richness marks a 123% increase from the 1981 review. The results have been made publicly available in the Catalog of Life in Taiwan database and the images recorded have been used to update species information in collembola.org. We also characterised morphological and genetic variations in the globular springtail species Papirioides jacobsoni Folsom, 1924 using DNA barcodes and highlighted potential research directions.
Published: 2022

50. Genetic and Functional Differences of Escherichia coli Strains from Colorectal Cancer Mucosal Tissues

Author: Zhiyuan Pan, Ni Han, Rui-Fu Yang, Lei Ding, Hong Gao, Yuxiao Chang, Chao Yang, Yujun Cui, Xiang Li, Fachao Zhi, and Yujing Bi
Subjects: Genetics, Environmental Engineering, General Computer Science, biology, Materials Science (miscellaneous), General Chemical Engineering, General Engineering, Energy Engineering and Power Technology, Genomics, Gut flora, biology.organism_classification, medicine.disease, medicine.disease_cause, Phenotype, Metagenomics, medicine, Cytokine secretion, Gene, Dysbiosis, Escherichia coli
Abstract: Colorectal cancer (CRC) is the third leading cancer globally. Metagenomics has been widely used to analyze the association between the gut microbiota and CRC based on bacterial genus- or species-level comparisons, providing evidence of dysbiosis in CRC development. However, this kind of analysis is unable to provide strain-level information for understanding the individual role of a species in CRC. Here, we used culturomics to isolate CRC mucosal samples and selected 158 Escherichia coli strains to reveal their differences in both genomics and functions by means of phylogenetic analysis and inflammatory induction based on cell and animal experiments. Through genomic comparison, these strains were divided into five phylogroups. The representative strains of each phylogroup significantly induced different levels of cytokine secretion by human leukemic monocyte (THP-1 cell)-based Transwell and animal experiments. Further bioinformatic analysis revealed different profiles of single-nucleotide polymorphisms, genes, and metabolic pathways in the different phylogroups, which can improve the current understanding of the phenotypic differences between these strains. The strain differences revealed in both genomics and functions indicate that the microbiota’s function at the strain level should be investigated in order to understand the interacting mechanisms between hosts and gut bacteria.
Published: 2022

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