151 results on '"Baraniak A"'
Search Results
2. Characteristics of ESBL-Producing Enterobacterales Colonizing the Gastrointestinal Tract in Patients Admitted to the Oncological Hospital
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Anna Baraniak, Maria Szymankiewicz, Elżbieta Stefaniuk, and Tomasz Nowikiewicz
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Adult ,medicine.medical_specialty ,Klebsiella pneumoniae ,Esbl production ,Microbial Sensitivity Tests ,Applied Microbiology and Biotechnology ,Microbiology ,beta-Lactamases ,03 medical and health sciences ,Internal medicine ,Enterobacterales ,Escherichia coli ,medicine ,Pulsed-field gel electrophoresis ,Humans ,In patient ,Escherichia coli Infections ,030304 developmental biology ,0303 health sciences ,Gastrointestinal tract ,biology ,030306 microbiology ,Klebsiella oxytoca ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,biology.organism_classification ,Hospitals ,Electrophoresis, Gel, Pulsed-Field ,Klebsiella Infections ,Gastrointestinal Tract ,bacteria ,Enterobacter cloacae - Abstract
We analyzed the prevalence and genetic characteristics of the extended-spectrum β-lactamases (ESBLs)-producing Enterobacterales isolated from adult patients hospitalized in the oncological center in 2019. Out of 9372 patients admitted to the hospital, 1373 had been in various medical facilities during the last year, which was an indication to perform a screening test for ESBL-producing Enterobacterales colonizing their gastrointestinal tract. In eighty-three patients (6.1%), 85 ESBL producers were detected. These isolates included the following: Escherichia coli (n = 67; 78.8%), Klebsiella pneumoniae (n = 14; 16.5%), Enterobacter cloacae cplx (n = 3; 3.5%), and Klebsiella oxytoca (n = 1; 1.2%). CTX-M-1-like enzymes were the most common ESBLs (n = 67; 78.8%). Two K. pneumoniae isolates (2/85; 2.4%) additionally produced New Delhi-metallo-β-lactamases (NDM). All isolates, except for K. oxytoca, were typed by pulsed-field gel electrophoresis (PFGE) and demonstrated high genetic diversity. The most prevalent phylogroups of E. coli were B2 group (n = 30; 44.8%), followed by A group (n = 25; 37.3%). These observations have motivated us to investigate the link between ESBL-E colonization and infection among patients with solid tumors.
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- 2021
3. Trigonella foenum-graecum seeds in treatment of metabolic syndrome
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Justyna Baraniak and Małgorzata Kania-Dobrowolska
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Trigonella ,biology ,Traditional medicine ,business.industry ,food and beverages ,fenugreek (trigonella foenum-graecum l.) ,Plant culture ,030209 endocrinology & metabolism ,biology.organism_classification ,medicine.disease ,metabolic syndrome ,SB1-1110 ,fenugreek seeds ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,medicine ,Metabolic syndrome ,fenugreek (Trigonella foenum-graecum L.), metabolic syndrome, fenugreek seeds ,business - Abstract
Fenugreek has a long tradition of use as a medicine and also has been commonly used as food in many countries. This plant is well known, especially in Asia. In Europe, fenugreek seeds are regarded as a traditional herbal medicine consumed in case of loss of appetite and in confirmed skin inflammations. Trigonella foenum-graecum seeds contain wide spectrum of different biologically active constituents which affect the properties of preparations produced from this plant. Numerous preclinical studies confirm the use of fenu-greek as a hypolipidemic and lowering blood glucose level drug. Unfortunately, there are not many clinical studies on fenugreek seeds in this area. Published studies often significantly differ in the amount and type of fenugreek seed preparation used. However, results of available scientific research are promising and suggest the possibility of using fenugreek seeds in metabolic syndrome therapy. Undoubtedly, further research is required to confirm such properties of Trigonella foenum-graecum.
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- 2020
4. Evaluation of ACE, α-glucosidase, and lipase inhibitory activities of peptides obtained by in vitro digestion of selected species of edible insects
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Ewelina Zielińska, Monika Karaś, Barbara Baraniak, and Anna Jakubczyk
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chemistry.chemical_classification ,0303 health sciences ,biology ,030309 nutrition & dietetics ,Peptide ,04 agricultural and veterinary sciences ,General Chemistry ,040401 food science ,Biochemistry ,Chemical synthesis ,Industrial and Manufacturing Engineering ,Hydrolysate ,In vitro ,03 medical and health sciences ,0404 agricultural biotechnology ,Enzyme ,chemistry ,Sephadex ,biology.protein ,Lipase ,IC50 ,Food Science ,Biotechnology - Abstract
The objective of this study was to examine the inhibition of the activity of enzymes associated with development of the metabolic syndrome by peptide fractions received from simulated gastrointestinal digestion and absorption of heat-treated edible insects. The inhibitory activities of insect-derived peptides were determined against key enzymes relevant to the metabolic syndrome such as the angiotensin-converting enzyme (ACE), pancreatic lipase, and α-glucosidase. After the in vitro absorption process, all hydrolysates showed high inhibitory activity; however, the most effective metabolic syndrome-inhibitory peptides were received after separation on Sephadex G10. The best results were found for peptide fractions obtained from Schistocerca gregaria. The highest enzymes inhibitory activities were obtained for peptide fractions from S. gregaria: boiled for ACE (IC50 3.95 µg mL−1), baked for lipase (IC50 9.84 µg mL−1), and raw for α-glucosiadase (IC50 1.89 µg mL−1) S. gregaria, respectively. Twelve sequences of peptides from the edible insects were identified and their chemical synthesis was carried out as well. Among the synthesized peptides, the KVEGDLK, YETGNGIK, AIGVGAIR, IIAPPER, and FDPFPK sequences of peptides exhibited the highest inhibitory activity. Generally, the heat treatment process applied to edible insects has a positive effect on the properties of the peptide fractions studied.
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- 2020
5. Immune Correlates of Protection Against Human Cytomegalovirus Acquisition, Replication, and Disease
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Ilona Baraniak, Sallie R. Permar, Daniele Lilleri, Matthew B. Reeves, Cody S. Nelson, and Paul D. Griffiths
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Human cytomegalovirus ,Immunogen ,Congenital cytomegalovirus infection ,Cytomegalovirus ,Supplement Articles ,Disease ,Virus Replication ,Virus ,Cytomegalovirus Vaccines ,Immune system ,medicine ,Humans ,Immunology and Allergy ,Viremia ,Immunity, Mucosal ,Disease Resistance ,Innate immune system ,biology ,business.industry ,Incidence ,Vaccination ,biochemical phenomena, metabolism, and nutrition ,medicine.disease ,Virus Shedding ,Infectious Diseases ,Cytomegalovirus Infections ,Host-Pathogen Interactions ,Immunology ,biology.protein ,Antibody ,business - Abstract
Human cytomegalovirus (HCMV) is the most common infectious cause of infant birth defects and an etiology of significant morbidity and mortality in solid organ and hematopoietic stem cell transplant recipients. There is tremendous interest in developing a vaccine or immunotherapeutic to reduce the burden of HCMV-associated disease, yet after nearly a half-century of research and development in this field we remain without such an intervention. Defining immune correlates of protection is a process that enables targeted vaccine/immunotherapeutic discovery and informed evaluation of clinical performance. Outcomes in the HCMV field have previously been measured against a variety of clinical end points, including virus acquisition, systemic replication, and progression to disease. Herein we review immune correlates of protection against each of these end points in turn, showing that control of HCMV likely depends on a combination of innate immune factors, antibodies, and T-cell responses. Furthermore, protective immune responses are heterogeneous, with no single immune parameter predicting protection against all clinical outcomes and stages of HCMV infection. A detailed understanding of protective immune responses for a given clinical end point will inform immunogen selection and guide preclinical and clinical evaluation of vaccines or immunotherapeutics to prevent HCMV-mediated congenital and transplant disease.
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- 2020
6. Does gene flow balance the effect of habitat fragmentation in a population of the hermit beetle <scp> Osmoderma barnabita </scp> ?
- Author
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Marek Przewoźny, Iwona Melosik, Małgorzata Rzepka, Tomasz Grzegorczyk, and Edward Baraniak
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education.field_of_study ,Habitat fragmentation ,Balance (accounting) ,Ecology ,Insect Science ,Population ,Osmoderma barnabita ,Biology ,education ,Ecology, Evolution, Behavior and Systematics ,Gene flow - Published
- 2020
7. Eidophasia insulella (Walsingham 1900) and Eidophasia hufnagelii (Zeller 1839), two rare European moths: description of genitalia confirms generic placement (Lepidoptera: Plutellidae)
- Author
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Edward Baraniak
- Subjects
biology ,eidophasia hufnagelii ,Fauna ,Zoology ,Plutella ,biology.organism_classification ,Lepidoptera genitalia ,taxonomy ,Geography ,plutellidae ,Plutellidae ,eidophasia insulella ,lcsh:Zoology ,Animal Science and Zoology ,Taxonomy (biology) ,Eidophasia ,lepidoptera ,lcsh:QL1-991 - Abstract
The taxonomic position of two species Plutella hufnagelii Zeller 1839 and Caunaca insulella Walsingham 1900, in the family Plutellidae: has been unclear. In current fauna studies, these species are usually placed in the genera Rhigognostis and Eidophasia respectively. In this paper, I address the taxonomic position of P. hufnagelli and C. insulella and describethe genitalia of both sexes. Analysis of genital structure confirms the correct placement of these species into the genus Eidophasia.
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- 2020
8. A global multinational survey of cefotaxime-resistant coliforms in urban wastewater treatment plants
- Author
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Yunho Lee, Manika Choudhury, Om Prakash, Donald Morrison, Hélène Guilloteau, Heidrun Mayrhofer, María Inmaculada Polo-López, Belen Esteban, Katarzyna Slipko, Leonardo Pantoja Munoz, Samira Nahim–Granados, José Manuel Guillén-Navarro, Marco Guida, Stela Krizanovic, Hemda Garelick, Pilar Caballero, Traore Ousmane, Marcos Quintela-Baluja, Sara Rodríguez-Mozaz, Helmut Bürgmann, Nikolina Udiković-Kolić, Carsten Ulrich Schwermer, Agnieszka Kalinowska, Milena Milaković, Ester Heath, Marta Piotrowska, Andrea Di Cesare, Célia M. Manaia, Ying Yang, Amy Pruden, Anne F.C. Leonard, Erica Donner, Tanel Telson, Christophe Merlin, Karin Beck, Ana Agüera, Anhony A Adegoke, Marie-Noëlle Pons, Bastian Herzog, Joana Abreu-Silva, Leonie Henn, Norbert Kreuzinger, Younggun Yoon, Stefanie Heß, Olga C. Nunes, Alice L. Petre, Despo Fatta-Kassinos, Gianluca Brunetti, Roberto B. M. Marano, Stella Michael, Thor A. Stenström, Ayella Maile-Moskowitz, Popi Karaolia, Joshua T. Bunce, Giovanni Libralato, Jérôme Ory, Yogesh Nimonkar, Alfieri Pollice, Aneta Luczkiewicz, Carlo Salerno, Andrew Scott, Shichun Zou, Antoni Oliver, Barbara Drigo, Isabel Martínez-Alcalá, Virginia Riquelme, Veljo Kisand, Anna Baraniak, William H. Gaze, Edward Topp, Edouard Jurkevitch, Isabel Henriques, Telma Fernandes, Eddie Cytryn, José Luis Balcázar, Marta Tacão, Thomas Schwartz, Thomas Jäger, Gianluca Corno, Mailis Laht, Thomas U. Berendonk, Roberto Rosal, Magdalena Popowska, Pawel Krzeminski, Connor L. Brown, Veritati - Repositório Institucional da Universidade Católica Portuguesa, Marano, R. B. M., Fernandes, T., Manaia, C. M., Nunes, O., Morrison, D., Berendonk, T. U., Kreuzinger, N., Telson, T., Corno, G., Fatta-Kassinos, D., Merlin, C., Topp, E., Jurkevitch, E., Henn, L., Scott, A., Hess, S., Slipko, K., Laht, M., Kisand, V., Di Cesare, A., Karaolia, P., Michael, S. G., Petre, A. L., Rosal, R., Pruden, A., Riquelme, V., Aguera, A., Esteban, B., Luczkiewicz, A., Kalinowska, A., Leonard, A., Gaze, W. H., Adegoke, A. A., Stenstrom, T. A., Pollice, A., Salerno, C., Schwermer, C. U., Krzeminski, P., Guilloteau, H., Donner, E., Drigo, B., Libralato, G., Guida, M., Burgmann, H., Beck, K., Garelick, H., Tacao, M., Henriques, I., Martinez-Alcala, I., Guillen-Navarro, J. M., Popowska, M., Piotrowska, M., Quintela-Baluja, M., Bunce, J. T., Polo-Lopez, M. I., Nahim-Granados, S., Pons, M. -N., Milakovic, M., Udikovic-Kolic, N., Ory, J., Ousmane, T., Caballero, P., Oliver, A., Rodriguez-Mozaz, S., Balcazar, J. L., Jager, T., Schwartz, T., Yang, Y., Zou, S., Lee, Y., Yoon, Y., Herzog, B., Mayrhofer, H., Prakash, O., Nimonkar, Y., Heath, E., Baraniak, A., Abreu-Silva, J., Choudhury, M., Munoz, L. P., Krizanovic, S., Brunetti, G., Maile-Moskowitz, A., Brown, C., Cytryn, E., The Robert H. Smith Faculty of Agriculture, Food and Environment, Department of Animal Sciences, The Hebrew University of Jerusalem (HUJ), Institute of Soil, Water and Environmental Sciences, Agricultural Research Organization - the Volcani Center, Centro de Química Fina e Biotecnologia (CQFB), Requimte, Universidade do Porto-Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Laboratory for Process Engineering, Environment, Biotechnology and Energy (LEPABE), Universidade do Porto, Edinburgh Napier University, Institute for Hydrobiology, Technische Universität Dresden = Dresden University of Technology (TU Dresden), Vienna University of Technology, Institute of Technology [Tartu, Estonia], University of Tartu, CNR Water Research Institute (IRSA), Consiglio Nazionale delle Ricerche (CNR), University of Cyprus (UCY), Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Agriculture and Agri-Food [Ottawa] (AAFC), Department of Biology, University of Western Ontario, University of Western Ontario (UWO), Estonian Environmental Research Centre (EKUK), Universidad de Alcalá - University of Alcalá (UAH), Virginia Tech [Blacksburg], Area de Quimica Inorganica - Centro de Investigacion en Energia Solar (CIESOL) (CIESOL), Universidad de Almería (UAL), Gdańsk University of Technology (GUT), University of Exeter Medical School, University of Exeter, Durban University of Technology, Universidad Nacional de Cuyo [Mendoza] (UNCUYO), Norwegian Institute for Water Research (NIVA), University of South Australia [Adelaide], Università degli studi di Napoli Federico II, Swiss Federal Insitute of Aquatic Science and Technology [Dübendorf] (EAWAG), Middlesex University [London], Universidade de Aveiro, Universidade de Coimbra [Coimbra], Universidad Católica San Antonio de Murcia (UCAM), Department of Applied Microbiology [Warsaw], Institute of Microbiology [Warsaw], Faculty of Biology [Warsaw], University of Warsaw (UW)-University of Warsaw (UW)-Faculty of Biology [Warsaw], University of Warsaw (UW)-University of Warsaw (UW), School of Engineering [Newcastle], Newcastle University [Newcastle], Plataforma Solar de Almeria – CIEMAT, Tabernas, Almeria, Laboratoire Réactions et Génie des Procédés (LRGP), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL), Rudjer Boskovic Institute [Zagreb], Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), CHU Clermont-Ferrand, Laboratorio EMATSA, Instituto Catalán de Investigación del Agua - ICRA (SPAIN) (ICRA), Institute of Functional Interfaces, Karlsruhe Institute of Technology (KIT), Sun Yat-Sen University [Guangzhou] (SYSU), Gwangju Institute of Science and Technology (GIST), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), NCMR, Jozef Stefan Institute [Ljubljana] (IJS), National Medicines Institute - Narodowy Instytut Leków [Warsaw] (NIL), Agricultural Research Organisation (ARO), Volcani Center, Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade do Porto = University of Porto, National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), University of Cyprus [Nicosia] (UCY), Agriculture and Agri-Food (AAFC), University of Naples Federico II = Università degli studi di Napoli Federico II, Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Marano, Robert BM, Fernandes, Telma, Manaia, Celia M, Nunes, Olga, Donner, Erica, Drigo, Barbara, Brunetti, Gianluca, and Cytryn, Eddie
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Cefotaxime ,010504 meteorology & atmospheric sciences ,Coliforms ,Antibiotic resistance ,Sewage ,Microorganismes -- Resistència als medicaments ,Wastewater treatment ,Wastewater ,010501 environmental sciences ,01 natural sciences ,Surveys and Questionnaires ,Microorganisms -- Drug resistance ,ComputingMilieux_MISCELLANEOUS ,lcsh:Environmental sciences ,General Environmental Science ,lcsh:GE1-350 ,6. Clean water ,Anti-Bacterial Agents ,3. Good health ,Europe ,Interdisciplinary Natural Sciences ,Health ,Sewage treatment ,Biotechnology ,medicine.drug ,Life sciences ,biology ,Aigua -- Reutilització ,Asia ,Quantification methods ,Aigües residuals -- Plantes de tractament ,Culture and Communities ,Water Purification ,Applied microbiology ,Water reuse ,Environmental health ,ddc:570 ,medicine ,Microbiology Research Group ,[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering ,antibiotic resistance ,coliforms ,ESBLs ,wastewater treatment ,wastewater reuse ,Sewage disposal plants ,Effluent ,0105 earth and related environmental sciences ,[SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health ,Coliform ,business.industry ,Australia ,Fecal coliform ,Antibiotic resistance, Coliforms, ESBLs, Wastewater treatment, Water reuse ,ESBL ,North America ,Environmental science ,business - Abstract
The World Health Organization Global Action Plan recommends integrated surveillance programs as crucial strategies for monitoring antibiotic resistance. Although several national surveillance programs are in place for clinical and veterinary settings, no such schemes exist for monitoring antibiotic-resistant bacteria in the environment. In this transnational study, we developed, validated, and tested a low-cost surveillance and easy to implement approach to evaluate antibiotic resistance in wastewater treatment plants (WWTPs) by targeting cefotaxime-resistant (CTX-R) coliforms as indicators. The rationale for this approach was: i) coliform quantification methods are internationally accepted as indicators of fecal contamination in recreational waters and are therefore routinely applied in analytical labs; ii) CTX-R coliforms are clinically relevant, associated with extended-spectrum β-lactamases (ESBLs), and are rare in pristine environments. We analyzed 57 WWTPs in 22 countries across Europe, Asia, Africa, Australia, and North America. CTX-R coliforms were ubiquitous in raw sewage and their relative abundance varied significantly (
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- 2020
9. Description of the female genitalia of Monochroa tekovella Kosorin, 2020 (Lepidoptera, Gelechioidea, Gelechiidae, Anomologini)
- Author
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Mariusz Mleczak, Peter Huemer, and Edward Baraniak
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biology ,Zoology ,Genitalia, Female ,Biodiversity ,Moths ,biology.organism_classification ,Gelechiidae ,Lepidoptera genitalia ,Monochroa ,Animals ,Animal Science and Zoology ,Female ,Gelechioidea ,Ecology, Evolution, Behavior and Systematics ,Taxonomy - Abstract
Baraniak, Edward, Mleczak, Mariusz, Huemer, Peter (2021): Description of the female genitalia of Monochroa tekovella Kosorin, 2020 (Lepidoptera, Gelechioidea, Gelechiidae, Anomologini). Zootaxa 5016 (4): 597-600, DOI: https://doi.org/10.11646/zootaxa.5016.4.10
- Published
- 2021
10. Is there a host-associated molecular and morphological differentiation between sympatrically occurring individuals of the invasive leaf miner Cameraria ohridella?
- Author
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Edward Baraniak, Julia Staszak, Urszula Walczak, Iwona Melosik, and Katarzyna Winnicka
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0106 biological sciences ,0301 basic medicine ,education.field_of_study ,Genetic diversity ,Ecology ,Host (biology) ,Wahlund effect ,Population ,Haplotype ,Biology ,Generalist and specialist species ,010603 evolutionary biology ,01 natural sciences ,03 medical and health sciences ,030104 developmental biology ,Genetic distance ,Evolutionary biology ,Insect Science ,Microsatellite ,education ,Agronomy and Crop Science ,Ecology, Evolution, Behavior and Systematics - Abstract
The leaf-miner mothCameraria ohridella,a pest in Central Europe, causes severe damage to trees. Host-associated differentiation (HAD) for this species has been suggested previously based on the occurrence of a specific mitochondrial haplotype. We assessed genetic diversity and population structure for sympatrically occurring individuals collected in association with two host species, Ohio buckeye (Aesculus glabra) and horse chestnut (Ae. hippocastanum), using six microsatellite loci (SSR) and mtDNA sequences that encode parts of cytochrome oxidase I and II. To infer population structure and assign individuals to clusters, we employed Bayesian clustering. We further characterized the relationships between genetic distance and geographical distance (IBD) in analyzed samples. Although our results derived from the SSR loci analyses demonstrating that there was no population substructuring caused by the hosts, we found evidence of differences in wing size, which might be attributed to the quality of food resources available to larvae. The population structure withK = 2 cannot be interpreted as the result of IBD; rather, it reflects a population differentiation due to demographic or genetic processes (e.g., an origin of invaders). Although genetic diversity was relatively high (He> 0.5), the population had a deficiency of heterozygotes (FIS > 0), which was most likely due to nonrandom mating and, possibly, a Wahlund effect. A star-like haplotype network and negative Tajima’s D support the genetic effect of bottleneck followed by population expansion. Based on presumably neutral markers, we conclude thatC. ohridellaappeared to be a good model for studying evolution toward a generalist invasive species, rather than HAD.
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- 2019
11. Towards endemicity: large-scale expansion of the NDM-1-producing Klebsiella pneumoniae ST11 lineage in Poland, 2015–16
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A, Baraniak, M, Machulska, D, Żabicka, E, Literacka, R, Izdebski, P, Urbanowicz, K, Bojarska, M, Herda, A, Kozińska, W, Hryniewicz, M, Gniadkowski, and G, Stepaniuk
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Microbiology (medical) ,Tunisia ,Endemic Diseases ,Genotype ,Peanut butter ,Klebsiella pneumoniae ,Microbial Sensitivity Tests ,beta-Lactamases ,Disease Outbreaks ,Microbiology ,Plasmid ,Communicable Diseases, Imported ,Pulsed-field gel electrophoresis ,Humans ,Pharmacology (medical) ,Typing ,Pharmacology ,Cross Infection ,biology ,Outbreak ,biology.organism_classification ,Anti-Bacterial Agents ,Bacterial Typing Techniques ,Klebsiella Infections ,Infectious Diseases ,Multilocus sequence typing ,Poland ,Genome, Bacterial ,Multilocus Sequence Typing - Abstract
ObjectivesIn 2015 and 2016 Poland recorded rapid proliferation of New Delhi MBL (NDM)-producing Enterobacterales, with at least 470 and 1780 cases, respectively. We addressed the roles of the Klebsiella pneumoniae ST11 NDM-1 outbreak genotype, already spreading in 2012–14, and of newly imported organisms in this increase.MethodsThe study included 2136 NDM-positive isolates identified between April 2015 and December 2016, following transfer of patients with K. pneumoniae ST147 NDM-1 from Tunisia to Warsaw in March 2015. The isolates were screened by PCR mapping for variants of blaNDM-carrying Tn125-like elements. Selected isolates were typed by PFGE and MLST. NDM-encoding plasmids were analysed by nuclease S1/hybridization, transfer assays, PCR-based replicon typing and PCR mapping.ResultsThe organisms were mainly K. pneumoniae containing the Tn125A variant of the ST11 epidemic lineage (n = 2094; ∼98%). Their representatives were of the outbreak pulsotype and ST11, and produced NDM-1, encoded by specific IncFII (pKPX-1/pB-3002cz)-like plasmids. The isolates were recovered in 145 healthcare centres in 13/16 administrative regions, predominantly the Warsaw area. The ‘Tunisian’ genotype K. pneumoniae ST147 NDM-1 Tn125F comprised 18 isolates (0.8%) from eight institutions. The remaining 24 isolates, mostly K. pneumoniae and Escherichia coli of diverse STs, produced NDM-1 or NDM-5 specified by various Tn125 derivatives and plasmids.ConclusionsThe K. pneumoniae ST11 NDM-1 outbreak has dramatically expanded in Poland since 2012, which may bring about a countrywide endemic situation in the near future. In addition, the so-far limited K. pneumoniae ST147 NDM-1 outbreak plus multiple NDM imports from different countries were observed in 2015–16.
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- 2019
12. Species composition, diversity, and the abundance of arthropods inhabiting burrows of the common hamster (Cricetus cricetusL.)
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Joanna Ziomek, Marek Przewoźny, Iwona Melosik, Katarzyna Winnicka, A Melke, K Komosiński, Edward Baraniak, P Celebias, and D J Gwiazdowicz
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0106 biological sciences ,biology ,Aleochara ,Rare species ,Zoology ,Parasitidae ,General Medicine ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,010602 entomology ,Abundance (ecology) ,Insect Science ,Cryptophagidae ,Mesostigmata ,Poecilochirus ,Agronomy and Crop Science ,Macrochelidae - Abstract
The is insufficient knowledge of arthropod communities occurring in specific microhabitats. In this study, we characterize the arthropod assemblages inhabiting burrows of the common hamster (Cricetus cricetusL.) and factors that determine their diversity and abundance. We tested the following hypotheses: (1) arthropod assemblages are associated with a particular dominant vegetation occurring in the vicinity of burrows; (2) a correlation exists between fine-scale geographic distances among burrows and assemblage dissimilarity; and (3) the type of trap influences the sampling success of captured arthropods. We found 73 morphospecies belonging to 16 families in 109 burrows, most of which were in the families Staphylinidae (Coleoptera) and Parasitidae (Arachnida: Acari: Mesostigmata). The most abundant families were Staphylinidae, Cryptophagidae (Coleoptera), Parasitidae, and Macrochelidae (Mesostigmata) (78.89%). Among the identified species, we foundAleochara irmgardis(Staphylinidae) andPoecilochirus sexclavatus(Parasitidae) which had not yet been reported in Poland, and several other rare species. Meat-baited traps captured 64.34% more individuals, which were more diverse and species-rich than the non-baited control traps, but the former was more selective for saprophages, necrophages, and coprophages. The burrows located in areas overgrown by triticale (a hybrid of wheat and rye) were inhabited by 69.86% of the identified arthropod species, and these also had the highest abundance (64.07%) in comparison with other habitats. However, differences in sample size biased our results toward and overestimate arthropods associated with this vegetation. This study underlines that the species composition detected in burrows was affected by the methods used and hamster preferences for a specific habitat rather than the geographic proximity of the burrows. More extensive sampling across multiple habitats will be necessary to confirm our findings.
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- 2019
13. One step closer to understanding the ecology of Cameraria ohridella (Lepidoptera: Gracillariidae): The effects of light conditions
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Marzenna Guzicka, Piotr Karolewski, Marian J. Giertych, Adrian Łukowski, Radosław Jagiełło, and Edward Baraniak
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0106 biological sciences ,Aesculus hippocastanum ,gracillariidae ,Leaf miner ,herbivore ,010603 evolutionary biology ,01 natural sciences ,Lepidoptera genitalia ,cameraria ohridella ,oocytes ,phenolics oxidative capacity ,leaf-miner ,Larva ,Herbivore ,biology ,Ecology ,Horse-chestnut leaf miner ,light conditions ,fungi ,aesculus hippocastanum ,plant-insect interactions ,biology.organism_classification ,Pupa ,010602 entomology ,QL1-991 ,Insect Science ,lepidoptera ,Gracillariidae ,Zoology - Abstract
Horse chestnut leaf miner (Cameraria ohridella) has achieved ecological success by colonizing the entire European range of its primary host, horse chestnut (Aesculus hippocastanum). This insect has attracted the attention of scientists, but its ecology is poorly understood. Here, we investigated the effects of varying degrees of light availability on the leaf morphology of horse chestnut saplings and the performance of C. ohridella. A pot experiment under greenhouse conditions was performed in which the photosynthetic photon flux density (PPFD) was reduced from full light by 50% (high light - HL) or 80% (low light - LL). Insect performance parameters were quantified (i.e., pupal mass, efficiency of conversion of utilised leaf tissue (ECU) and potential fecundity). Compared with HL leaflets those from LL were characterised by higher contents of nitrogen and water but lower total phenolics. The oxidative capacity of phenolics (at pH ≈ 10, common in the lepidopteran gut) was low and did not differ in the two treatments. Compared with those collected from HL leaves, the mines of those collected from leaves of plants grown under LL conditions were larger in area but the leaf mass utilized by larvae was similar. Pupae were heavier in LL than in HL conditions, and ECU was higher in LL. The potential fecundity of females was not sensitive for experimental treatment. We conclude that (1) reduced light had a strong beneficial effect on the performance of C. ohridella and (2) phenolics in A. hippocastanum leaf tissues are a poor defence against this herbivore.
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- 2019
14. Potentially Bioaccessible Phenolic and Antioxidant Potential of Fresh and Stored Lentil Sprouts—Effect of Lactobacillus plantarum 299v Enrichment
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Anna Jakubczyk, Urszula Gawlik-Dziki, Ireneusz Kapusta, Małgorzata Sikora, Michał Świeca, and Barbara Baraniak
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030309 nutrition & dietetics ,Metal ions in aqueous solution ,Radical ,Pharmaceutical Science ,antioxidant activity ,phenolic compounds ,Antioxidant potential ,Chemical Fractionation ,Lactobacillus plantarum ,Antioxidants ,Article ,lentil ,Analytical Chemistry ,law.invention ,storage ,lcsh:QD241-441 ,03 medical and health sciences ,chemistry.chemical_compound ,Probiotic ,0404 agricultural biotechnology ,Phenols ,lcsh:Organic chemistry ,law ,Tandem Mass Spectrometry ,Drug Discovery ,Chelation ,Food science ,Physical and Theoretical Chemistry ,Chromatography, High Pressure Liquid ,0303 health sciences ,ABTS ,biology ,Chemistry ,Plant Extracts ,Probiotics ,Organic Chemistry ,Extraction (chemistry) ,04 agricultural and veterinary sciences ,biology.organism_classification ,040401 food science ,bioaccessibility ,Chemistry (miscellaneous) ,Molecular Medicine ,Lens Plant - Abstract
The phenolic and antioxidant potential of potentially bioaccessible fractions of lentil sprouts was studied. Sprouts were cocultivated with a probiotic to obtain a new functional product and further stored in cool conditions. The fraction obtained after buffer extraction and gastric digestion had higher content of phenolics compared to the control (by 20% and 46%, respectively), however, a 9% decrease was observed in samples obtained after gastrointestinal digestion. After gastrointestinal digestion, the highest content of phenolics (278 µg/g d.w.) was determined in the fresh control sprouts. Compounds neutralizing ABTS and hydroxyl radicals, chelating metal ions, and exhibiting strong reducing power were effectively released after gastrointestinal digestion (e.g., the values of the gastrointestinal digestibility index for chelating power and ability to quench hydroxyl radicals significantly exceeded 1 in all studied samples). It was proved that the enrichment of sprouts with a probiotic and further storage significantly improved the antioxidant potential, compared to the fresh control sprouts, an increase by 45% and 10% was determined after the gastric and gastrointestinal digestion, respectively. Lentil sprouts enriched with L. plantarum 299v may be a new functional product characterized by the high antioxidant capacity of the potentially bioaccessible fraction.
- Published
- 2021
15. Epidemic Territorial Spread of IncP-2-Type VIM-2 Carbapenemase-Encoding Megaplasmids in Nosocomial Pseudomonas aeruginosa Populations
- Author
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Anna Baraniak, Paweł Urbanowicz, Marek Gniadkowski, Radosław Izdebski, Elżbieta Literacka, Jaroslav Hrabak, and Ibrahim Bitar
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Biology ,Integron ,medicine.disease_cause ,beta-Lactamases ,Integrons ,Epidemiology and Surveillance ,03 medical and health sciences ,Plasmid ,Bacterial Proteins ,Pulsed-field gel electrophoresis ,medicine ,Humans ,Pseudomonas Infections ,Pharmacology (medical) ,Epidemics ,Phylogeny ,030304 developmental biology ,Pharmacology ,Genetics ,Cross Infection ,0303 health sciences ,Genetic diversity ,Phylogenetic tree ,030306 microbiology ,Pseudomonas aeruginosa ,Hospitals ,Anti-Bacterial Agents ,Electrophoresis, Gel, Pulsed-Field ,Multiple drug resistance ,Infectious Diseases ,biology.protein ,Multilocus sequence typing ,Poland ,Multilocus Sequence Typing - Abstract
In 2003 to 2004, the first five VIM-2 metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) isolates with an In4-like integron, In461 (aadB-bla(VIM-2)-aadA6), on conjugative plasmids were identified in three hospitals in Poland. In 2005 to 2015, MPPA expanded much in the country, and as many as 80 isolates in a collection of 454 MPPA (∼18%) had In461, one of the two most common MBL-encoding integrons. The organisms occurred in 49 hospitals in 33 cities of 11/16 main administrative regions. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) classified them into 55 pulsotypes and 35 sequence types (STs), respectively, revealing their remarkable genetic diversity overall, with only a few small clonal clusters. S1 nuclease/hybridization assays and mating of 63 representative isolates showed that ∼85% of these had large In461-carrying plasmids, ∼350 to 550 kb, usually self-transmitting with high efficiency (∼10(−1) to 10(−2) per donor cell). The plasmids from 19 isolates were sequenced and subjected to structural and single-nucleotide-polymorphism (SNP)-based phylogenetic analysis. These formed a subgroup within a family of IncP-2-type megaplasmids, observed worldwide in pseudomonads from various environments and conferring resistance/tolerance to multiple stress factors, including antibiotics. Their microdiversity in Poland arose mainly from acquisition of different accessory fragments, as well as new resistance genes and multiplication of these. Short-read sequence and/or PCR mapping confirmed the In461-carrying plasmids in the remaining isolates to be the IncP-2 types. The study demonstrated a large-scale epidemic spread of multidrug resistance plasmids in P. aeruginosa populations, creating an epidemiological threat. It contributes to the knowledge on IncP-2 types, which are interesting research objects in resistance epidemiology, environmental microbiology, and biotechnology.
- Published
- 2021
16. Remote light-activation of native Orai channels
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James H. Baraniak, Sarah A. Kazzaz, Yandong Zhou, and Donald L. Gill
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ORAI1 Protein ,Lasers ,Membrane Proteins ,Cell Biology ,Computational biology ,Biology ,Research Highlight ,Mice ,Animals ,Calcium ,Light activation ,Calcium Channels ,Calcium Signaling ,Stromal Interaction Molecule 1 ,Molecular Biology - Abstract
Ca
- Published
- 2021
17. Molecular and genomic epidemiology of VIM/IMP-like metallo-β-lactamase-producing Pseudomonas aeruginosa genotypes in Poland
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Waleria Hryniewicz, Radosław Izdebski, D. Żabicka, Anna Baraniak, P Urbanowicz, and Marek Gniadkowski
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Microbiology (medical) ,Genotype ,Microbial Sensitivity Tests ,Biology ,medicine.disease_cause ,Integron ,beta-Lactamases ,Phylogenetics ,Inosine Monophosphate ,medicine ,Pulsed-field gel electrophoresis ,Humans ,Pharmacology (medical) ,Pseudomonas Infections ,Phylogeny ,Pharmacology ,Genetics ,Molecular Epidemiology ,Phylogenetic tree ,Pseudomonas aeruginosa ,Genomics ,Resistome ,Anti-Bacterial Agents ,Infectious Diseases ,biology.protein ,Multilocus sequence typing ,Poland ,Multilocus Sequence Typing - Abstract
Objectives To identify key factors of the expansion of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) in Poland, focusing on the role of clonal epidemic(s). Methods MPPA isolates were typed by PFGE, followed by MLST. blaVIM/IMP MBL genes were amplified and sequenced within class 1 integrons. Their location was assessed by S1 nuclease-hybridization assays. Short-read WGS was performed, and genomes were subjected to SNP-based phylogenetic and resistome analyses. Results Of 1314 MPPA isolates collected in 2005–15 from 212 hospitals, 454 representatives were selected. The isolates belonged to 120 pulsotypes and 52 STs, of which ST235 (∼31%), ST111 (∼17%), ST273 (∼16%) and ST654 (∼9%) prevailed, followed by ST244, ST17, ST395, ST175 and ST1567. The isolates produced seven VIM variants (97.5%) and four IMPs encoded by 46 integrons, most of which were observed only or mainly in Poland. Around 60% of the isolates resulted from (inter)regional clonal outbreaks of 10 individual ST235, ST111, ST273 and ST654 genotypes. The phylogenetic analysis of 163 genomes revealed heterogeneity of ST235 and ST111 populations, arising from transnational circulation and on-site differentiation of several clades/branches. Contrarily, ST273 and ST654 formed relatively homogeneous and apparently Poland-specific lineages, and a unique ST273 genotype with integron In249 was the most expansive organism. Conclusions Together with a previous report on self-transmissible In461-carrying IncP-2-type plasmids, this study revealed the molecular/genomic background of the rapid MPPA increase in Poland in 2001–15, evidencing multi-clonal spread as its leading factor. Numerous novel/specific MPPA characteristics were identified.
- Published
- 2021
18. Characterisation of Biologically Active Hydrolysates and Peptide Fractions of Vacuum Packaging String Bean (Phaseolus vulgaris L.)
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Ewelina Zielińska, Konrad A. Szychowski, Anna Jakubczyk, Beata Rutkowska, Piotr Stanikowski, Urszula E. Binduga, Monika Karaś, Kamila Rybczyńska-Tkaczyk, Barbara Baraniak, and Magdalena Dziedzic
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Health (social science) ,food.ingredient ,vacuum-packing ,in vitro digestion ,DPPH ,string bean ,Peptide ,Plant Science ,lcsh:Chemical technology ,Health Professions (miscellaneous) ,Microbiology ,Hydrolysate ,String bean ,chemistry.chemical_compound ,food ,lcsh:TP1-1185 ,Food science ,Lipase ,chemistry.chemical_classification ,nutritional potential ,ABTS ,biology ,food and beverages ,biology.organism_classification ,Squamous carcinoma ,chemistry ,bioactive peptides ,biology.protein ,Phaseolus ,Food Science - Abstract
The aim of the study is to characterise biologically active hydolysates and peptide fractions obtained from vacuum-packed string beans (Phaseolus vulragis L.) (PB). Unpacked beans were a control sample. The influence on human squamous carcinoma cell line SCC-15 (ATCC CRL-1623) was determined. Packed bean (PB) and unpacked bean (UB) extracts were found to exert no effect on the tongue squamous carcinoma cells. The results of the study indicated that the packing process contributed to the retention of protein, soluble dietary fibre, and free sugar (2.36, 3.5, and 1.79 g/100 d.m., respectively). PB was characterised by higher antioxidant activity (expressed as neutralisation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS ABTS•+) and 2,2-diphenyl-1-picrylhydrazyl (DPPH·) free radicals) as well as Fe2+ chelation and reducing power (IC50 = 54.56, 0.46, 3.85 mg mL−1; 0.088 A700/peptide content, respectively) than the UB samples before hydrolysis. The hydrolysis process enhanced these properties. The IC50 value of lipase and α-amylase inhibitory activity of the hydrolysates obtained from UB was reduced. The PB and UB fractions exhibited a certain level of antimicrobial activity against S. aureus and E. coli. Candida albicans were not sensitive to these peptide fractions.
- Published
- 2020
19. Characteristics of New Peptides GQLGEHGGAGMG, GEHGGAGMGGGQFQPV, EQGFLPGPEESGR, RLARAGLAQ, YGNPVGGVGH, and GNPVGGVGHGTTGT as Inhibitors of Enzymes Involved in Metabolic Syndrome and Antimicrobial Potential
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Anna Jakubczyk, Sławomir Lewicki, Urszula Złotek, Paula Ćwiek, Kamila Rybczyńska-Tkaczyk, and Barbara Baraniak
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Pharmaceutical Science ,Peptide ,Inhibitory postsynaptic potential ,Article ,Analytical Chemistry ,lcsh:QD241-441 ,03 medical and health sciences ,0404 agricultural biotechnology ,lcsh:Organic chemistry ,Anti-Infective Agents ,Drug Discovery ,Cytotoxic T cell ,Physical and Theoretical Chemistry ,Lipase ,inhibitory ,IC50 ,030304 developmental biology ,chemistry.chemical_classification ,Metabolic Syndrome ,0303 health sciences ,antimicrobial activity ,biology ,Organic Chemistry ,04 agricultural and veterinary sciences ,Antimicrobial ,040401 food science ,Enzyme assay ,Enzyme ,chemistry ,Biochemistry ,Salmonella enteritidis ,Chemistry (miscellaneous) ,cytotoxic properties ,biology.protein ,peptides ,Molecular Medicine ,alpha-Amylases - Abstract
The aim of this study was to determine the cytotoxic properties, influence on enzyme activity involved in metabolic syndrome, and antimicrobial activity of synthetic peptides with GQLGEHGGAGMG, GEHGGAGMGGGQFQPV, EQGFLPGPEESGR, RLARAGLAQ, YGNPVGGVGH, and GNPVGGVGHGTTGT sequences. Peptides have no cytotoxic effect on cells. The highest inhibitory effect on angiotensin converting enzyme I was noted for peptide GT-14 (IC50 = 525.63 µ, g/mL). None of the tested peptides had an influence on &alpha, glucosidase. The highest &alpha, amylase and lipase inhibitory activity was noted for GG-12 (IC50 = 56.72 and 60.62 µ, g/mL, respectively). The highest lipoxidase inhibitory activity was determined for peptide ER-13 (IC50 = 84.35 µ, g/mL). Peptide RQ-9 was characterized by the highest COX inhibitory activity (0.31 and 4.77 µ, g/mL for COX-1 and COX-2, respectively). Only peptide RQ-9 inhibited S. enteritidis ATCC 4931 growth (42%&ndash, 48%) in all tested concentrations (15.62&ndash, 250 mg/mL).
- Published
- 2020
20. Polymorphism analysis of the Gly972Arg IRS-1 and Gly1057Asp IRS-2 genes in obese pregnant women
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Agnieszka Seremak-Mrozikiewicz, Justyna Baraniak, Małgorzata Kania-Dobrowolska, Marlena Wolek, Bogusław Czerny, Aleksandra Górska, Izabela Uzar, Piotr Olbromski, Anna Bogacz, and Magdalena Sienko
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Adult ,medicine.medical_specialty ,Waist ,Genotype ,Type 2 diabetes ,Overweight ,Weight Gain ,Polymorphism, Single Nucleotide ,Young Adult ,Endocrinology ,Insulin resistance ,Pregnancy ,Insulin receptor substrate ,Internal medicine ,Medicine ,Humans ,Genetic Predisposition to Disease ,Obesity ,Genetic Association Studies ,biology ,business.industry ,medicine.disease ,Insulin receptor ,biology.protein ,Insulin Receptor Substrate Proteins ,Animal Science and Zoology ,Female ,medicine.symptom ,Insulin Resistance ,business ,Developmental Biology - Abstract
Genes encoding insulin receptor substrates IRS-1 and IRS-2 perform key functions in the insulin pathway. Numerous authors have suggested that single-nucleotide polymorphism (SNP) changes in the DNA sequence may be associated with the development of obesity, insulin resistance and type 2 diabetes. The Gly972Arg polymorphism of the IRS-1 gene and the Gly1057Asp polymorphism of the IRS-2 gene are believed to be associated with the occurrence of insulin resistance and obesity according to many sources. The aim of our study was to investigate the influence of these polymorphisms on the clinical parameters and to assess their correlations in obese Polish pregnant women. A total of 154 pregnant Caucasian women from the Wielkopolska region were analyzed: 78 diagnosed with overweight or obesity (study group) and 76 with normal body mass (controls). The analysis of the polymorphisms was performed using the PCR-restriction fragment length polymorphism (PCR-RFLP) method. The IRS-2 Gly1057Asp polymorphism revealed no significant correlations with excessive weight gain during pregnancy. The analysis of the IRS-1 Gly972Arg polymorphism showed an association with obesity between the study and control groups (GG-80.77%, GR-17.95%, RR-1.28% vs GG-94.74%, GR-5.26%; p = 0.023). We also observed slightly increased BMI values and higher values of the waist and hip circumference before pregnancy in the case of the IRS-1 Gly972Arg polymorphism. The analysis of the clinical and anthropometric parameters demonstrated no significant relationships between the genotypes of the polymorphic variants of the IRS-1 and IRS-2 genes but suggested an association between the IRS-1 Gly972Arg polymorphism and the risk for obesity.
- Published
- 2020
21. VIM/IMP carbapenemase-producing Enterobacteriaceae in Poland: epidemic Enterobacter hormaechei and Klebsiella oxytoca lineages
- Author
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Alicja Sekowska, Marek Gniadkowski, Anna Baraniak, Waleria Hryniewicz, Eugenia Gospodarek-Komkowska, Dorota Zabicka, and Radosław Izdebski
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DNA, Bacterial ,0301 basic medicine ,Microbiology (medical) ,Klebsiella pneumoniae ,030106 microbiology ,Microbial Sensitivity Tests ,Carbapenem-resistant enterobacteriaceae ,beta-Lactamases ,Integrons ,Microbiology ,03 medical and health sciences ,Bacterial Proteins ,Enterobacteriaceae ,Drug Resistance, Multiple, Bacterial ,Pulsed-field gel electrophoresis ,Humans ,Pharmacology (medical) ,Epidemics ,Pharmacology ,biology ,Enterobacteriaceae Infections ,Klebsiella oxytoca ,Enterobacter ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,biology.organism_classification ,Anti-Bacterial Agents ,Bacterial Typing Techniques ,Carbapenem-Resistant Enterobacteriaceae ,Infectious Diseases ,Carbapenems ,bacteria ,Multilocus sequence typing ,Poland ,Enterobacter cloacae ,Multilocus Sequence Typing ,Plasmids - Abstract
Objectives To analyse VIM/IMP-type MBL-producing Enterobacteriaceae isolates identified in Poland during 2006-12. Methods Isolates were typed by PFGE, followed by MLST. blaVIM/IMP genes were amplified and sequenced within class 1 integrons. Their plasmidic versus chromosomal location was assessed by nuclease S1 and I-CeuI plus hybridization experiments. Plasmids were characterized by transfer assays and PCR-based replicon typing. Results One hundred and nineteen VIM/IMP-positive Enterobacteriaceae cases were reported in Poland from the first case in 2006 until 2012. The patients were in 54 hospitals and were infected or colonized by 121 organisms, including Enterobacter cloacae complex (n = 64), Klebsiella oxytoca (n = 23), Serratia marcescens (n = 20) and Klebsiella pneumoniae (n = 11). The isolates represented numerous pulsotypes and mainly original STs, and carried eight integrons with blaVIM-1-like genes (blaVIM-1/-4/-28/-37/-40; n = 101), three with blaVIM-2 variants (blaVIM-2/-20; n = 17) and one with blaIMP-19 (n = 3). Six integrons were new, and five and two formed prevalent families of In238-like (n = 96) and In1008-like (n = 16) elements, respectively. In238 (aacA4-blaVIM-4rpt) and In1008 (blaVIM-2-aacA4) had been originally observed in Polish Pseudomonas aeruginosa, suggestive of their transfer to enterobacteria, followed by spread and diversification. Four organisms have disseminated inter-regionally, i.e. Enterobacter hormaechei ST90 with plasmidic In238/In238a integrons (n = 36), K. oxytoca ST145 with a chromosomal In237-like element (n = 18) and two subclones of E. hormaechei ST89 with In1008- or In238-type variants (n = 8 and n = 7, respectively). Conclusions The epidemiology of VIM/IMP-producing Enterobacteriaceae in Poland has revealed a remarkable number of specific or novel characteristics of the organisms, with some possible links to other mid-southern European countries.
- Published
- 2018
22. Maladaptive host choice by an alien leaf miner Phyllonorycter leucographella (Lepidoptera: Gracillariidae) has the potential to limit its invasiveness
- Author
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Roma Żytkowiak, Urszula Walczak, Piotr Karolewski, Michał Bogdziewicz, and Edward Baraniak
- Subjects
0106 biological sciences ,Specific leaf area ,gracillariidae ,Leaf miner ,Introduced species ,phyllonorycter leucographella ,Biology ,medicine.disease_cause ,010603 evolutionary biology ,01 natural sciences ,host selection ,invasive species ,Infestation ,Botany ,medicine ,infestation rate ,insect development ,Host (biology) ,fungi ,food and beverages ,alien leaf miner ,Native plant ,biology.organism_classification ,010602 entomology ,QL1-991 ,Insect Science ,PEST analysis ,lepidoptera ,Gracillariidae ,Zoology ,specific leaf area - Abstract
Alien phytophagous insects are often introduced along with their host plants, creating opportunities for troublesome invasions. Yet, not all of them are able to successfully colonize novel host plants. In this study, we investigated host selection by the alien leaf miner Phyllonorycter leucographella (Zeller, 1850) on both its original host and novel host plants in the insect's alien range. We predicted that this insect's percentage infestation of the original host would be positively related to its specific leaf area (SLA), because high-SLA leaves are nutritious and have thin cuticles, traits related to high offspring developmental success. We further hypothesized that this host selection process would apply in the selection of novel host plants. Our results show that this leaf miner selects leaves of its original host plant, Pyracantha coccinea, according to their SLA values. The SLA value was also positively related to the probability of P. leucographella infesting and successfully developing on novel host plants. The selection of high-SLA plants by the moth leads to a high developmental success on novel host plants in the first (summer) generation, but it is likely to be maladaptive in the second (overwintering) generation, because in temperate Europe, high SLA values are associated with deciduous plants that shed their leaves in autumn. It is likely that the apparent maladaptive selection of novel host plants by P. leucographella reduces the invasiveness of this pest by preventing its establishment on native plants.
- Published
- 2018
23. Identification of antioxidant and anti-inflammatory peptides obtained by simulated gastrointestinal digestion of three edible insects species (Gryllodes sigillatus,Tenebrio molitor,Schistocerca gragaria)
- Author
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Ewelina Zielińska, Barbara Baraniak, and Monika Karaś
- Subjects
chemistry.chemical_classification ,Antioxidant ,biology ,medicine.drug_class ,DPPH ,medicine.medical_treatment ,Peptide ,04 agricultural and veterinary sciences ,cricket ,biology.organism_classification ,040401 food science ,Chemical synthesis ,Industrial and Manufacturing Engineering ,Anti-inflammatory ,cricket.player ,Hydrolysate ,chemistry.chemical_compound ,0404 agricultural biotechnology ,chemistry ,medicine ,Schistocerca ,Gryllodes sigillatus ,Food science ,Food Science - Abstract
The aim of this study was to determine the effect of heat treatment of edible insects on antioxidant and anti‐inflammatory activities of peptide fractions from hydrolysates obtained by in vitro gastrointestinal digestion thereof. Identification of bioactive peptides from the edible insects and their chemical synthesis were carried out as well. The highest antiradical activity against ABTS•⁺ and DPPH• was noted for the peptide fraction from the Gryllodes sigillatus protein preparation (EC₅₀ value 2.75 and 6.91 μg mL⁻¹, respectively). This fraction exhibited the strongest LOX and COX‐2 inhibitory activity (IC₅₀ value 0.13 and 0.26 μg mL⁻¹, respectively). The peptide fraction from the Tenebrio molitor protein preparation showed the highest Fe²⁺ chelating ability (EC₅₀ value 2.21 μg mL⁻¹) and the highest reducing power (0.198). The heat treatment process has a positive effect on the antioxidant and anti‐inflammatory properties of peptides. All identified and synthesised peptides from insect protein showed antioxidant and anti‐inflammatory activity.
- Published
- 2018
24. Comparison of functional properties of edible insects and protein preparations thereof
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Monika Karaś, Ewelina Zielińska, and Barbara Baraniak
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biology ,Chemistry ,04 agricultural and veterinary sciences ,cricket ,biology.organism_classification ,040401 food science ,cricket.player ,0404 agricultural biotechnology ,Emulsion ,Schistocerca ,Gryllodes sigillatus ,Food science ,Solubility ,Protein solubility ,Food Science - Abstract
This study investigated the functional properties of three species of edible insects: Gryllodes sigillatus, Schistocerca gregaria, and Tenebrio molitor. The water and oil holding capacity, solubility, and foaming and emulsion properties were evaluated. The protein solubility showed minimum values at pH 5. The highest water and oil holding capacity was noticeable for the T. molitor protein preparation (3.95 g/g) and for the G. sigilltus protein preparation (3.33 g/g), respectively. The G. sigillatus protein preparation also showed the highest foaming capacity, foam stability, and emulsion activity (99.0%, 92.0%, and 72.62%, respectively), while the protein preparation from S. gregaria exhibited the highest emulsion stability (51.31%). This study has shown that whole insects and protein preparations thereof can be suitable for development of new food formulations.
- Published
- 2018
25. Impact ofCameraria ohridellaonAesculus hippocastanumgrowth and long-term effects of trunk injection with pesticides
- Author
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Urszula Walczak, Grzegorz Iszkuło, Edward Baraniak, Marian J. Giertych, Piotr Karolewski, and Radosław Jagiełło
- Subjects
0106 biological sciences ,Aesculus hippocastanum ,Insect outbreak ,Cameraria ohridella ,Trunk injection ,Pesticide ,Biology ,01 natural sciences ,Horse chestnut ,Potential fecundity ,010602 entomology ,Horticulture ,Insect Science ,Tree health ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
Defoliation caused annually by Cameraria ohridella has a considerable impact on the general condition of horse chestnut trees. We investigated the consequences of tree endotherapy against this inse...
- Published
- 2018
26. Influence of salidroside, a neuroactive compound of Rhodiola rosea L., on alcohol tolerance development in rats
- Author
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Agnieszka Gryszczyńska, Anna Krajewska-Patan, Bogna Geppert, Piotr Mularczyk, Ewa Kamińska, Małgorzata Kania-Dobrowolska, Przemysław Ł. Mikołajczak, Marcin Ożarowski, Justyna Baraniak, Michał Szulc, and Radosław Kujawski
- Subjects
0301 basic medicine ,alcohol tolerance ,Pharmacology ,lcsh:Plant culture ,Neuroprotection ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,In vivo ,medicine ,lcsh:SB1-1110 ,Alcohol tolerance ,Ethanol ,biology ,ethanol-induced hypothermia ,sedative effect ,Salidroside ,Hypothermia ,biology.organism_classification ,salidroside ,rats ,030104 developmental biology ,Rhodiola rosea ,chemistry ,Pharmacodynamics ,dose-dependent manner ,medicine.symptom ,030217 neurology & neurosurgery - Abstract
Summary Introduction: In recent years, the search for potential neuroprotective properties of salidroside and its ability to influence the activity of nervous system become the subject of intense studies of many research groups. None of these studies, however, include an attempt to determine the effect of salidroside on the course of alcohol tolerance in vivo. Objective: The aim of this study was to investigate the ability of salidroside to inhibit the development of alcohol tolerance in rats, determining whether the effect of its action may occur in a dose-dependent manner, reducing both metabolic and central tolerance without affecting body temperature in control rats. Methods: Male Wistar rats were injected daily with ethanol at a dose of 3 g/kg for 9 consecutive days to produce ethanol tolerance. Salidroside in two doses (4.5 mg/kg and 45 mg/kg b.w.) or vehiculum was administered orally. On the 1st, 3rd, 5th and 8th day a hypothermic effect of ethanol was measured, while the loss of righting reflex procedure was performed on the 2nd, 4th, 6th and 7th day. On the 9th day rats were treated with salidroside, sacrificed 1 h after ethanol injections and blood was collected for blood-ethanol concentration measurement. Results: Salidroside at a dose of 45 mg/kg inhibited the development of tolerance to hypothermic and sedative effects of ethanol, whereas insignificant elevation of blood-ethanol concentration was observed. The dose of 4.5 mg/kg b.w. had minimal effect, only small inhibition of tolerance to hypothermic action was observed. Salidroside affected neither body mass growth nor body temperature in non-alcoholic (control) rats. Conclusions: Results of the study indicate that salidroside at a dose of 45 mg/kg inhibited the development of tolerance to the hypothermic effect of ethanol. Observed inhibition of tolerance to the sedative effect of ethanol seems to be associated with salidroside influence on the central nervous system. A comprehensive explanation of the abovementioned observations requires further pharmacological and pharmacodynamic studies.
- Published
- 2018
27. Use of a genetically informed population viability analysis to evaluate management options for Polish populations of endangered beetle Cerambyx cerdo L. (1758) (Coleoptera, Cerambycidae)
- Author
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Melosik Iwona, Przewoźny Marek, Baraniak Edward, Winnicka Katarzyna, and Staszak Julia
- Subjects
0106 biological sciences ,0301 basic medicine ,Genetic diversity ,education.field_of_study ,Ecology ,biology ,Population size ,Population ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,03 medical and health sciences ,030104 developmental biology ,Population viability analysis ,Genetic drift ,Animal ecology ,Insect Science ,Cerambyx cerdo ,Animal Science and Zoology ,education ,Longhorn beetle ,Nature and Landscape Conservation - Abstract
A paucity of genetic information and the drastic decline in population size of the beetle Cerambyx cerdo has made this species a high priority for research and conservation management. The state-listed beetle, a saproxylic insect associated with oaks, has a discontinuous range, with larger and more connected populations in southern Europe and small and isolated populations in the continent’s central and northern parts. Here, we used seven microsatellite loci and one DNA fragment of the mitochondrial gene COI to examine the population structure, genetic diversity, and contemporary gene flow between two Polish populations of the beetle. A population viability analysis summarizing collected genetic data as well as field records and species-specific information was performed to investigate the probability of the populations’ persistence over 20 years under different simulation scenarios. Genetic drift due to spatial isolation and bottleneck(s) is probably a major evolutionary force responsible for a low number of haplotypes and lower gene diversity in these populations as compared to the neighboring Czech populations. Despite a large geographic distance between the Polish populations, genetic differentiation between them was low, which could reflect shared ancestral polymorphism and stochasticity of retained alleles rather than the homogenizing effect of gene flow. Differences among probabilities of extinction over 20 years were detected between populations, and, in the worst-case scenarios, one population will disappear within four generations. Conservation efforts must focus on supplementation, habitat restoration, and post-release monitoring. The results of our study provided information that can be incorporated into future management actions to aid in the conservation of the beetle.
- Published
- 2017
28. Enterobacteriaceae producing OXA-48-like carbapenemases in Poland, 2013–January 2017
- Author
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R, Izdebski, A, Baraniak, D, Zabicka, M, Machulska, P, Urbanowicz, J, Fiett, E, Literacka, K, Bojarska, A, Kozinska, B, Zieniuk, W, Hryniewicz, M, Gniadkowski, and B, Wcisło-Wach
- Subjects
0301 basic medicine ,Microbiology (medical) ,Klebsiella pneumoniae ,030106 microbiology ,Microbial Sensitivity Tests ,Carbapenem-resistant enterobacteriaceae ,beta-Lactamases ,03 medical and health sciences ,Plasmid ,Bacterial Proteins ,Enterobacteriaceae ,Escherichia coli ,Humans ,Pharmacology (medical) ,Typing ,Escherichia coli Infections ,Pharmacology ,Genetics ,biology ,Escherichia coli Proteins ,Enterobacteriaceae Infections ,biology.organism_classification ,Bacterial Typing Techniques ,Klebsiella Infections ,Infectious Diseases ,Multilocus sequence typing ,Poland ,Mobile genetic elements ,Enterobacter cloacae ,Multilocus Sequence Typing - Abstract
Objectives To analyse OXA-48 (OXA-48/181)-type carbapenemase-producing Enterobacteriaceae reported in Poland from 2013 until January 2017. Methods Bacterial isolates were typed by PFGE and MLST. Genes coding for OXA-48/181 types and other β-lactamases were amplified and sequenced. Mobile elements with blaOXA-48/181-like genes were PCR mapped. blaOXA-48/181-carrying plasmids were characterized by nuclease S1-hybridization profiling, transfer assays and PCR-based replicon typing, while the chromosomal location of the genes was confirmed by the I-CeuI analysis. Results Fifty-four isolates from 52 patients in 20 hospitals (14 cities) were included, in 14 cases having probable foreign origins indicated. The organisms were genetically diverse and represented numerous pandemic clones, including Klebsiella pneumoniae ST395 (n = 23), ST11, ST15 and ST101, Escherichia coli ST38, ST410 and ST648, and Enterobacter cloacae complex ST78. These produced OXA-48 (n = 49), OXA-181 (n = 4) or OXA-232 (n = 1). One of five K. pneumoniae ST395 pulsotypes caused a multicentre outbreak with 18 cases, which significantly contributed to the total number of patients. Depending on the variant, the blaOXA-48/181-like genes were parts of the Tn1999-, Tn2013- or Tn2016-like transposons, with blaOXA-48 found in an ISEcp1-associated module (Tn2016-like) for the first time. Three genotypes, including E. coli ST38, had chromosomal blaOXA-48 genes, while others carried transmissible IncL (∼60 kb; blaOXA-48; n = 30), IncM (∼80-95 kb; blaOXA-48; n = 4), IncX3 (∼50 kb; blaOXA-181; n = 4) or non-typeable (∼90-160 kb; blaOXA-48 or blaOXA-232) plasmids. Conclusions Even though OXA-48/181 producers seem to occur infrequently in Poland, their epidemiology has been marked by various phenomena, namely multiple imports, several limited transmissions plus one larger clonal outbreak, and possible plasmid transfers.
- Published
- 2017
29. Case Report of COVID-19 after Full Vaccination: Viral Loads and Anti-SARS-CoV-2 Antibodies
- Author
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Robert Gromadka, Iza Ksiazek, Jarosław Walory, Jan Gawor, Magdalena Komiazyk, and Anna Baraniak
- Subjects
Medicine (General) ,viral loads ,Coronavirus disease 2019 (COVID-19) ,biology ,business.industry ,viruses ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Clinical Biochemistry ,COVID-19 ,Case Report ,Virology ,Virus ,Vaccination ,mRNA vaccine ,R5-920 ,Immune system ,Sore throat ,medicine ,biology.protein ,medicine.symptom ,Antibody ,business ,Viral load ,anti-SARS-CoV-2 antibodies - Abstract
The introduction of effective vaccines against SARS-CoV-2 is expected to prevent COVID-19. However, sporadic cases of infection in vaccinated persons have been reported. We describe a case of a double-dose vaccinated woman with COVID-19. All stages of infection were observed, from no identification of virus, then the start of the infection, a high viral load, coming out of viraemia, and finally no detection of the virus. Despite the high viral load, the woman demonstrated mild COVID-19 symptoms, manifested only by a sore throat. The antibody results showed that she produced both post-infectious and post-vaccination immune responses. Phylogenetic analysis of the obtained viral genome sequence indicated that the virus belonged to the UK SARS-CoV-2 lineage B.1.1.7 (GR 501Y.V1; 20I/S:501Y.V1; Alpha variant).
- Published
- 2021
30. Occurrence of Beta-Lactamases in Colistin-Resistant Enterobacterales Strains in Poland – a Pilot Study
- Author
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Stefan Tyski, Aleksandra Kozińska, Izabela Waśko, Anna Baraniak, and Elżbieta M. Stefaniuk
- Subjects
Microbiology (medical) ,colistin-resistance ,carbapenemases ,Klebsiella pneumoniae ,Avibactam ,Ceftazidime ,Pilot Projects ,QH426-470 ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Microbiology ,Meropenem ,beta-Lactamases ,chemistry.chemical_compound ,Enterobacterales ,Enterobacteriaceae ,Drug Resistance, Bacterial ,polycyclic compounds ,Genetics ,medicine ,Escherichia coli ,Vaborbactam ,biology ,Colistin ,Enterobacteriaceae Infections ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,biology.organism_classification ,QR1-502 ,ESBLs ,chemistry ,Poland ,medicine.drug - Abstract
Sixty-five colistin-resistant Enterobacterales isolates recovered from different clinical specimens were analyzed. The strains were collected in 12 hospitals all over Poland within a period of nine months. Strains were analyzed for eight genes from the mcr family. The presence of mcr-1 gene was detected in three Escherichia coli strains. The 45/65 isolates were identified as ESBL producers. CTX-M-1-like enzymes were the most common ESBLs (n = 40). One E. coli and seven Klebsiella pneumoniae strains produced carbapenemases, with the NDM being produced by five isolates. Among all the strains tested, four and five were resistant to new drugs meropenem/vaborbactam and ceftazidime/avibactam, respectively.
- Published
- 2021
31. Ecophysiological aspects of the interaction between Cameraria ohridella and Guignardia aesculi on Aesculus hippocastanum
- Author
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Radosław Jagiełło, Marian J. Giertych, Urszula Walczak, Piotr Łakomy, Piotr Karolewski, Edward Baraniak, and Jolanta Behnke-Borowczyk
- Subjects
0106 biological sciences ,Aesculus hippocastanum ,010602 entomology ,Guignardia aesculi ,Cameraria ohridella ,Botany ,Forestry ,Plant Science ,Biology ,010603 evolutionary biology ,01 natural sciences - Published
- 2017
32. Evaluation of the Carba NP test for carbapenemase detection in Enterobacteriaceae, Pseudomonas spp. and Acinetobacter spp., and its practical use in the routine work of a national reference laboratory for susceptibility testing
- Author
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Anna Skoczynska, Waleria Hryniewicz, Anna Baraniak, D. Żabicka, Marek Gniadkowski, M. Herda, and E Literacka
- Subjects
0301 basic medicine ,Microbiology (medical) ,Susceptibility testing ,030106 microbiology ,Drug resistance ,Microbial Sensitivity Tests ,Reference laboratory ,Polymerase Chain Reaction ,Sensitivity and Specificity ,beta-Lactamases ,Microbiology ,Agar plate ,03 medical and health sciences ,Bacterial Proteins ,Enterobacteriaceae ,Drug Resistance, Multiple, Bacterial ,Pseudomonas ,Humans ,biology ,Acinetobacter ,Routine work ,General Medicine ,biology.organism_classification ,Infectious Diseases ,Original Article - Abstract
The aim of this study was to evaluate the Carba NP test (and CarbAcineto) for the detection of carbapenemases in Enterobacteriaceae, Pseudomonas spp. and Acinetobacter spp., and to assess its usefulness in the routine work of the National Reference Centre for Susceptibility Testing (NRCST) in Poland. The evaluation of the Carba NP/CarbAcineto tests was carried out on a group of 81 Enterobacteriaceae, Pseudomonas spp. and Acinetobacter spp. isolates producing KPC-, NDM-, VIM-, IMP- or OXA-48, -23, -24/40, -58-type carbapenemases, and on 26 carbapenemase-negative strains cultivated on a broad panel of microbiological media. Subsequently, the performance of the Carba NP/CarbAcineto tests was assessed on 1282 isolates of Enterobacteriaceae, Pseudomonas spp. and Acinetobacter spp. from Polish hospitals, submitted to the NRCST during a 9-month period in 2014. The Carba NP/CarbAcineto results were compared with other phenotypic tests and/or polymerase chain reaction (PCR). The impact of the media on the results of the Carba NP/CarbAcineto tests was observed, with the Columbia blood agar yielding the highest sensitivity and clarity of the results. Furthermore, the Carba NP/CarbAcineto tests were included in the NRCST routine procedure for carbapenemase identification. The sensitivity and specificity of the Carba NP test were 95.8% and 93.3%, respectively, for Enterobacteriaceae, and 97.5% and 99.0%, respectively, for Pseudomonas spp. The sensitivity of the CarbAcineto test for Acinetobacter spp. was 88.9%. This study confirmed the usefulness of the Carba NP/CarbAcineto tests for the rapid detection of various types of carbapenemases.
- Published
- 2017
33. Larval food affects oviposition preference, female fecundity and offspring survival inYponomeuta evonymellus
- Author
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Joanna Mucha, Urszula Walczak, Piotr Karolewski, Marian J. Giertych, Adrian Łukowski, and Edward Baraniak
- Subjects
0106 biological sciences ,Prunus serotina ,Larva ,Ecology ,biology ,Host (biology) ,Offspring ,fungi ,Zoology ,biology.organism_classification ,Fecundity ,010603 evolutionary biology ,01 natural sciences ,010602 entomology ,Yponomeuta evonymellus ,Insect Science ,parasitic diseases ,Botany ,Plant species ,Prunus padus - Abstract
1. Yponomeuta evonymellus is a monophagous moth that feeds on Prunus padus which is native to Europe. In recent years, larval feeding and egg clusters have also been observed on non-native Prunus serotina plants; however, survival of larvae on this new host is very low. 2. The objective of the present study was to determine how the feeding of larvae on each of the two host plants impacts oviposition, offspring survival and fecundity in Y. evonymellus. Our hypothesis was that, under controlled conditions, females will lay eggs on the host on which they fed as larvae. We also hypothesised that the lower survival of young larvae feeding on P. serotina was due to the smaller buds and leaves present in this species, relative to those of P. padus. 3. A dual-choice experiment conducted under laboratory conditions demonstrated that females preferentially chose to oviposit on the plant species on which they fed as larvae. In the experiment, potential fecundity and offspring survival were significantly higher on P. padus than on P. serotina. The reduced performance of Y. evonymellus on P. serotina was correlated with a smaller bud mass and volume, lower leaf mass and surface area, and difficulty in constructing a protective tent against unfavourable weather conditions. 4. In summary, the identity of the host plant species during larval feeding determines adult oviposition preference for that host species. The survival of larvae on P. serotina growing in the nature is low, but for phenology-related reasons.
- Published
- 2017
34. Survival, body mass and potential fecundity of the invasive moth Cameraria ohridella (Lepidoptera: Gracillariidae) on its original host plant Aesculus hippocastanum and Aesculus glabra
- Author
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Piotr Zduniak, Urszula Walczak, and Edward Baraniak
- Subjects
0106 biological sciences ,Aesculus hippocastanum ,animal structures ,gracillariidae ,Leaf miner ,Aesculus glabra ,010603 evolutionary biology ,01 natural sciences ,Invasive species ,invasive species ,Lepidoptera genitalia ,cameraria ohridella ,Botany ,leaf miner ,aesculus glabra ,Larva ,biology ,fungi ,food and beverages ,aesculus hippocastanum ,biology.organism_classification ,Pupa ,010602 entomology ,QL1-991 ,Insect Science ,lepidoptera ,herbivore performance ,Gracillariidae ,Zoology - Abstract
Performance of the invasive horse-chestnut leaf miner, Cameraria ohridella Deschka & Dimic, 1986 (Lepidoptera: Gracillariidae), was studied on two host plants: the white-flowering horse-chestnut Aesculus hippocastanum L. and the Ohio buckeye Aesculus glabra Willd. C. ohridella developed successfully on both host plants; however, mine density and survival were much higher on A. hippocastanum than on A. glabra. The pupal mass and potential fecundity were strongly affected by the host plant on which the larvae fed. On A. hippocastanum pupae were significantly heavier and females more fecund than those on A. glabra. Furthermore, on both host plants there was a significant positive correlation between the number of oocytes in ovaries and pupal body mass, and as a consequence, heavier females produced more eggs. Our study demonstrates that the mine density, survival, pupal mass and potential fecundity were significantly lower on A. glabra than on A. hippocastanum. The observed lower performance of C. ohridella on the exotic host plant was assumed to be due to its poor food quality (nutritional and chemical composition).
- Published
- 2017
35. Effect of carboxymethyl cellulose/candelilla wax coating containing ascorbic acid on quality of walnut (Juglans regiaL.) kernels
- Author
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Emil Zięba, Tomasz Skrzypek, Dariusz Kowalczyk, and Barbara Baraniak
- Subjects
biology ,Chemistry ,04 agricultural and veterinary sciences ,engineering.material ,Candelilla wax ,biology.organism_classification ,Ascorbic acid ,040401 food science ,Industrial and Manufacturing Engineering ,Carboxymethyl cellulose ,Hydrolysis ,0404 agricultural biotechnology ,Coating ,Lipid oxidation ,Emulsion ,medicine ,engineering ,Organic chemistry ,Food science ,Food Science ,medicine.drug ,Juglans - Abstract
Summary Walnut kernels were coated with carboxymethyl cellulose (CMC)-based emulsion with and without l-ascorbic acid (AA). The thickness of the coatings formed on the walnuts ranged from 12 to 360 μm. The results obtained from the thiobarbituric acid-reactive substance analysis showed that the coating retarded lipid oxidation in walnuts during long-term storage. The antioxidative action of the CMC-based coating was not enhanced by incorporation of AA. On the other hand, the coating with AA slightly delayed hydrolytic rancidity of walnuts. More smooth and homogenous surface of walnuts coated with AA led to higher gloss. The acidifying/reductive action of AA lightened the colour of walnuts. Coating (dipping and drying) resulted in partial loss of aromaticity of walnuts.
- Published
- 2017
36. Effect of carboxymethylcellulose/candelilla wax coating containing potassium sorbate on microbiological and physicochemical attributes of pears
- Author
-
Monika Kordowska-Wiater, Emil Zięba, Dariusz Kowalczyk, and Barbara Baraniak
- Subjects
Monilinia fructigena ,Potassium sorbate ,biology ,Cold storage ,Rhizopus nigricans ,Ripening ,04 agricultural and veterinary sciences ,Horticulture ,Candelilla wax ,engineering.material ,biology.organism_classification ,040401 food science ,body regions ,chemistry.chemical_compound ,0404 agricultural biotechnology ,chemistry ,Coating ,Botany ,Postharvest ,engineering ,Food science - Abstract
The main objective of this study was to assess the efficacy of a coating composed of carboxymethylcellulose (CMC), candelilla wax and potassium sorbate (KS) as a post-cold-storage treatment to prevent fungal infections in pears stored under simulated retail display conditions. Moreover, the effect of coating on the physiology and biochemistry of pears was investigated. The coating was very effective against Botrytis cinerea and Monilinia fructigena, while Rhizopus nigricans was the most resistant to KS. The KS-free coating also delayed the fungal growth rate, probably due to modification of the gaseous atmosphere within the fruit tissues. Coated pears showed slower ripening than the uncoated samples, as indicated by unaffected green skin color and inhibited loss of firmness. Unfortunately, coating induced anaerobic respiration and the symptoms of superficial scald in pears. Overall, the results showed that KS can be added into a coating formulation to control fungal growth; however, CMC-based emulsion is not a suitable carrier for KS, when coating is intended to be applied to pears exposed to postharvest cold storage.
- Published
- 2017
37. Comparison of antioxidant activities of fractionated extracts from seedlings and herb of Chelidonium majus L. using DPPH, ABTS and FRAP methods
- Author
-
Radosław Kujawski, Marcin Ożarowski, Wojciech Białas, Aurelia Pietrowiak, Przemysław Mikołajczak, Agnieszka Seremak-Mrozikiewicz, M Gorska-Paukszta, Waldemar Buchwald, Justyna Baraniak, Bogdan Kędzia, Agnieszka Gryszczyńska, and Anna Krajewska-Patan
- Subjects
0301 basic medicine ,Antioxidant ,food.ingredient ,DPPH ,phytochemical analysis ,medicine.medical_treatment ,Pharmacology toxicology ,antioxidant activity ,lcsh:Plant culture ,alkaloids ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,food ,herbal extract ,statistical analysis ,medicine ,Chelidonium ,heterocyclic compounds ,ABTS ,lcsh:SB1-1110 ,Chromatography ,biology ,Traditional medicine ,greater celandine ,Chelidonium majus ,biology.organism_classification ,030104 developmental biology ,chemistry ,Herb ,flavonoids ,FRAP ,030217 neurology & neurosurgery - Abstract
Summary Introduction: Our study is a part of a trend of studies on the antioxidative properties of Chelidonium majus extracts or their fractions suggesting that antioxidant activities may depend on total flavonoid and/or alkaloid contents. Objective: This study focused on the examination of antioxidative activities of full water extract, non-protein fraction and protein fraction of the extract from aerial parts of mature plants and young seedlings. Methods: Total flavonoid and alkaloid contents were evaluated by spectrometric methods. Quantitative determination of chelidonine, coptisine, sanquinarine, berberine was made by HPLC-UV. The antioxidative activities were evaluated using (1) 2,2-diphenyl-1-picrylhydrazyl (DPPH), (2) 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging and (3) ferric reducing antioxidant power (FRAP) methods. Results: All concentrations of herb extracts exhibited higher antioxidant capacities than extract from seedlings. Two antioxidant tests (DPPH, FRAP) showed that full water extract from herb had the highest antioxidant activity, while its non-protein fraction and protein fraction showed lower antioxidant activity. It was found that the full water extract from herb contained the highest concentrations of flavonoids and alkaloids when compared with other samples. Conclusion: Our findings suggest that chelidonine and coptisine especially could be responsible for the observed changes in the extract antioxidant activity, because these alkaloids were determined in the highest concentration in full water extract from herb. It cannot be also excluded that the observed variables values between extracts and their fractions from herb or from seedlings may also be the result of interactions between flavonoids and other chemical compounds.
- Published
- 2016
38. The remote allosteric control of Orai channel gating
- Author
-
James H. Baraniak, Donald L. Gill, Kenneth P K Lee, Mohamed Trebak, Yandong Zhou, and Robert M. Nwokonko
- Subjects
0301 basic medicine ,ORAI1 Protein ,Gating ,Physical Chemistry ,Biochemistry ,Fluorophotometry ,Spectrum Analysis Techniques ,Single Channel Recording ,0302 clinical medicine ,Fluorescence Resonance Energy Transfer ,Electron Microscopy ,Biology (General) ,Membrane Electrophysiology ,Calcium signaling ,Microscopy ,Crystallography ,Physics ,Drosophila Melanogaster ,General Neuroscience ,Eukaryota ,Animal Models ,Condensed Matter Physics ,Lipids ,Insects ,Chemistry ,Bioassays and Physiological Analysis ,Experimental Organism Systems ,Spectrophotometry ,Physical Sciences ,Crystal Structure ,Drosophila ,General Agricultural and Biological Sciences ,Research Article ,Anions ,Arthropoda ,QH301-705.5 ,Allosteric regulation ,Biology ,Research and Analysis Methods ,Green Fluorescent Protein ,General Biochemistry, Genetics and Molecular Biology ,Protein–protein interaction ,03 medical and health sciences ,Model Organisms ,Solid State Physics ,Animals ,Secretion ,Calcium Signaling ,Stromal Interaction Molecule 1 ,Ion channel ,Ions ,General Immunology and Microbiology ,Endoplasmic reticulum ,Electrophysiological Techniques ,Organisms ,Biology and Life Sciences ,Proteins ,Electron Cryo-Microscopy ,Invertebrates ,Luminescent Proteins ,030104 developmental biology ,Membrane protein ,Animal Studies ,Biophysics ,Calcium ,Calcium Channels ,030217 neurology & neurosurgery - Abstract
The Orai channel is characterized by voltage independence, low conductance, and high Ca2+ selectivity and plays an important role in Ca2+ influx through the plasma membrane (PM). How the channel is activated and promotes Ca2+ permeation is not well understood. Here, we report the crystal structure and cryo-electron microscopy (cryo-EM) reconstruction of a Drosophila melanogaster Orai (dOrai) mutant (P288L) channel that is constitutively active according to electrophysiology. The open state of the Orai channel showed a hexameric assembly in which 6 transmembrane 1 (TM1) helices in the center form the ion-conducting pore, and 6 TM4 helices in the periphery form extended long helices. Orai channel activation requires conformational transduction from TM4 to TM1 and eventually causes the basic section of TM1 to twist outward. The wider pore on the cytosolic side aggregates anions to increase the potential gradient across the membrane and thus facilitate Ca2+ permeation. The open-state structure of the Orai channel offers insights into channel assembly, channel activation, and Ca2+ permeation., The structure of a constitutively active mutant of the fruit fly Orai calcium influx channel reveals a conformational transduction pathway upon channel activation and suggests an anion-assisted mechanism for calcium permeation.
- Published
- 2019
39. Pseudomonas aeruginosa with NDM-1, DIM-1 and PME-1 β-lactamases, and RmtD3 16S rRNA methylase, encoded by new genomic islands
- Author
-
D. Żabicka, Anna Baraniak, Waleria Hryniewicz, Marek Gniadkowski, G Ziółkowski, P Urbanowicz, and Radosław Izdebski
- Subjects
Pharmacology ,Microbiology (medical) ,Genetics ,Methyltransferase ,Genomic Islands ,Pseudomonas aeruginosa ,β lactamases ,RNA ,Beta-lactamase NDM-1 ,Microbial Sensitivity Tests ,Biology ,Ribosomal RNA ,16S ribosomal RNA ,medicine.disease_cause ,Genome ,beta-Lactamases ,Anti-Bacterial Agents ,Infectious Diseases ,Bacterial Proteins ,RNA, Ribosomal, 16S ,medicine ,Humans ,Pharmacology (medical) ,Carboxylic Ester Hydrolases - Published
- 2019
40. Proteus mirabilis Producing the OXA-58 Carbapenemase in Poland
- Author
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Anna Baraniak, Waleria Hryniewicz, A Schneider, D. Żabicka, Marek Gniadkowski, Paweł Urbanowicz, Radosław Izdebski, Małgorzata Herda, and E Literacka
- Subjects
Pharmacology ,Infectious Diseases ,biology ,Enterobacterales ,Pharmacology (medical) ,biology.organism_classification ,Letter to the Editor ,Proteus mirabilis ,Microbiology - Published
- 2019
41. Light conditions affect the performance of Yponomeuta evonymellus on its native host Prunus padus and the alien Prunus serotina
- Author
-
Urszula Walczak, Edward Baraniak, Marian J. Giertych, Piotr Karolewski, and Adrian Łukowski
- Subjects
Male ,0106 biological sciences ,Food Chain ,Light ,Moths ,010603 evolutionary biology ,01 natural sciences ,Invasive species ,Trees ,Lepidoptera genitalia ,Species Specificity ,Botany ,Animals ,Herbivory ,Prunus padus ,Prunus serotina ,Larva ,biology ,Host (biology) ,General Medicine ,biology.organism_classification ,Fecundity ,Pupa ,Fertility ,Insect Science ,Female ,Prunus ,Introduced Species ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
The bird cherry ermine moth, Yponomeuta evonymellus L., is considered an obligatory monophagous insect pest that feeds only on native European Prunus padus L. In recent years, however, increased larval feeding on alien P. serotina Ehrh. has been observed. In both species, general defoliation is extensive for shade grown trees, whereas it is high in P. padus, but very low in P. serotina, when trees are grown in full light conditions. The aim of the present study was to identify how the plant host species and light conditions affect the performance of Y. evonymellus. The influence of host species and light condition on their growth and development, characterized by the parameters of pupation, adult eclosion, body mass, potential fecundity, and wing size, was measured in a 2 × 2 experimental design (two light treatments, two hosts). In comparison with high light (HL) conditions, a greater percentage of pupation and a longer period and less dynamic adult emerge was observed under low light (LL) conditions. The effect of host species on these parameters was not significant. In contrast, mass, fecundity and all of the studied wing parameters were higher in larvae that grazed on P. padus than on P. serotina. Similarly the same parameters were also higher on shrubs in HL as compared with those grown under LL conditions. In general, light conditions, rather than plant species, were more often and to a greater extent, responsible for differences in the observed parameters of insect development and potential fecundity.
- Published
- 2016
42. Crystallographic studies of the complex of human HINT1 protein with a non-hydrolyzable analog of Ap4A
- Author
-
Agnieszka Krakowiak, Janina Baraniak, Renata Kaczmarek, Rafał Dolot, Aleksandra Sęda, and Barbara Nawrot
- Subjects
0301 basic medicine ,Nerve Tissue Proteins ,Biology ,Crystallography, X-Ray ,Biochemistry ,Protein Structure, Secondary ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Structural Biology ,Hydrolase ,medicine ,Humans ,Binding site ,Molecular Biology ,Histidine ,Binding Sites ,Hydrolysis ,Polyphosphate ,Triad (anatomy) ,General Medicine ,Crystallography ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,030220 oncology & carcinogenesis ,ddc:540 ,Apoproteins ,Ap4A ,Dinucleoside Phosphates ,Monoclinic crystal system - Abstract
International journal of biological macromolecules 87, 62 - 69(2016). doi:10.1016/j.ijbiomac.2016.02.047, Histidine triad nucleotide-binding protein 1 (HINT1) represents the most ancient and widespread branch in the histidine triad proteins superfamily. HINT1 plays an important role in various biological processes, and it has been found in many species. Here, we report the first structure (at a 2.34 Å resolution) of a complex of human HINT1 with a non-hydrolyzable analog of an Ap4A dinucleotide, containing bis-phosphorothioated glycerol mimicking a polyphosphate chain, obtained from a primitive monoclinic space group P21 crystal. In addition, the apo form of hHINT1 at the space group P21 refined to 1.92 Å is reported for comparative studies., Published by Elsevier, New York, NY [u.a.]
- Published
- 2016
43. Impact of cold on the immune system of burying beetle,Nicrophorus vespilloides(Coleoptera: Silphidae)
- Author
-
Arkadiusz Urbański, Grzegorz Rosiński, Elżbieta Czarniewska, and Edward Baraniak
- Subjects
0106 biological sciences ,0301 basic medicine ,biology ,Ecology ,media_common.quotation_subject ,Zoology ,Insect ,biology.organism_classification ,Nicrophorus vespilloides ,010603 evolutionary biology ,01 natural sciences ,General Biochemistry, Genetics and Molecular Biology ,Silphidae ,03 medical and health sciences ,030104 developmental biology ,Immune system ,Insect Science ,Hemolymph ,Burying beetle ,Desiccation ,Agronomy and Crop Science ,Ecology, Evolution, Behavior and Systematics ,Overwintering ,media_common - Abstract
Insect overwintering is one of the most astonishing phases of the insect life cycle. Despite vast amounts of knowledge available about the physiological mechanisms of this phenomenon, the impact of stress factors on insect immune system functioning during the winter is still unknown. The aim of this study is to analyze how low temperatures influence the immune system of the beetle Nicrophorus vespilloides. The results show that the beetle's immune system is differently modulated by cold induced in laboratory settings than that which occurs in natural conditions. Among beetles cultured in conditions similar to summer, low temperatures, did not influence the number of circulating haemocytes, phenoloxidase activity, haemocytes morphology, and percentage ratio of haemocyte types. In these beetles, differences were noted only in the ability of haemocytes to perform phagocytosis. Individuals acclimated in natural conditions in autumn had a higher level of humoral response and a different percentage ratio of haemocyte types. During the winter period, the number of haemocytes in the beetles decreased, but the percentage ratio of phagocytic haemocytes increased. Furthermore, we noted an increase of phenoloxidase activity. Our study also showed mitotic divisions of haemocytes in haemolymph collected from burying beetles after cold exposure and from burying beetles collected from natural conditions during autumn and winter. Differences in response to low temperatures in laboratory conditions and the natural environment suggest that the simultaneous presence of other stress factors during winter such as desiccation and starvation have a significant influence on the activity of burying beetle's immune system.
- Published
- 2016
44. 3′-O- and 5′-O-Propargyl Derivatives of 5-Fluoro-2′-Deoxyuridine: Synthesis, Cytotoxic Evaluation and Conformational Analysis
- Author
-
Piotr Ruszkowski, Daniel Baranowski, Dagmara Baraniak, and Jerzy Boryski
- Subjects
0301 basic medicine ,Stereochemistry ,Molecular Conformation ,Sulforhodamine B ,Antineoplastic Agents ,01 natural sciences ,Biochemistry ,HeLa ,03 medical and health sciences ,chemistry.chemical_compound ,Floxuridine ,Cell Line, Tumor ,Neoplasms ,Genetics ,medicine ,Humans ,Cytotoxic T cell ,biology ,010405 organic chemistry ,General Medicine ,biology.organism_classification ,Deoxyuridine ,0104 chemical sciences ,030104 developmental biology ,chemistry ,Cancer cell ,Propargyl ,Molecular Medicine ,Female ,Nucleoside ,medicine.drug - Abstract
A series of new 3'-O- and 5'-O-propargyl derivatives of 5-fluoro-2'-deoxyuridine (1-4) was synthesized by means of propargyl reaction of properly blocked nucleosides (2,4), followed by the deprotection reaction with ammonium fluoride. The synthesized propargylated 5-fluoro-2'-deoxyuridine analogues (1-4) were evaluated for their cytotoxic activity in three human cancer cell lines: cervical (HeLa), oral (KB) and breast (MCF-7), using the sulforhodamine B (SRB) assay. The highest activity and the best SI coefficient in all of the investigated cancer cells were displayed by 3'-O-propargyl-5-fluoro-2'-deoxyuridine (1), and its activity was higher than that of the parent nucleoside. The other new compounds exhibited moderate activity in all of the used cell lines.
- Published
- 2016
45. Selected species of edible insects as a source of nutrient composition
- Author
-
Anna Jakubczyk, Barbara Baraniak, Ewelina Zielińska, Kamila Rybczyńska, and Monika Karaś
- Subjects
chemistry.chemical_classification ,Mealworm ,biology ,food and beverages ,cricket ,biology.organism_classification ,cricket.player ,Hydrolysate ,Amino acid ,Nutrient ,chemistry ,Botany ,Composition (visual arts) ,Schistocerca ,Gryllodes sigillatus ,Food science ,Food Science ,Polyunsaturated fatty acid - Abstract
The aim of this study was to determine the nutritive value of edible insects and their in vitro cytotoxicity assays. The content of protein, fat, carbohydrates, ash, fiber, minerals, amino acids, and fatty acids in adult cricket (Gryllodes sigillatus), larvae of mealworm (Tenebrio molitor), and adult locust (Schistocerca gregaria) were analyzed. The protein content ranged from 52.35 to 76%. The fat percentage was in the range of 12.97–24.7%. Energy contribution varied from 1821 to 1896 kJ/100 g. Their amino acid profile was compared with the WHO/FAO/UNU Pattern (WHO, 2007). The highest degree of hydrolysis (DH) was noted in baked Gryllodes sigillatus (37.76%). All species were very rich in magnesium, copper, iron, and zinc and the mineral content was compared to recommended daily intakes (mg/day). The hydrolysates from raw, cooked, and baked insects were significantly stimulated or inhibited proliferation of human skin fibroblasts CRL-2522.
- Published
- 2015
46. Effect of cold storage on the potentially bioaccessible isoflavones and antioxidant activities of soybean sprouts enriched with Lactobacillus plantarum 299v
- Author
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Michał Świeca, Urszula Gawlik-Dziki, Urszula Złotek, Barbara Baraniak, Monika Kordowska-Wiater, and Ireneusz Kapusta
- Subjects
0106 biological sciences ,Antioxidant ,biology ,Chemistry ,medicine.medical_treatment ,food and beverages ,Cold storage ,04 agricultural and veterinary sciences ,Isoflavones ,biology.organism_classification ,040401 food science ,01 natural sciences ,Cool storage ,chemistry.chemical_compound ,Antioxidant capacity ,0404 agricultural biotechnology ,Polyphenol ,010608 biotechnology ,medicine ,Genistin ,Food science ,Lactobacillus plantarum ,Food Science - Abstract
The study was focused on determination of the effect of cool storage on the polyphenol profile and antioxidant capacity of potentially bioaccessible phenolics from control and probiotic-rich soybean sprouts. Malonyl glycitin and malonyl genistin were found to be the dominant isoflavones in the sprouts. The contents of the isoflavones after cold storage were generally equal or higher than in the fresh sprouts. The highest reducing power was determined for the potentially bioaccessible fraction from the probiotic-rich sprouts (9.9 mg TE/g, approx. a 4-fold increase compared to the control). The storage positively influenced the reducing potential; after gastric digestion, there was a 61% and 59% increase for the control and probiotic-rich sprouts, respectively. The addition of probiotic bacteria did not increase the phenolic content; however, it improved the antioxidant capacity. Importantly, the antioxidants in the proposed sprouted food were characterized by high storage stability. Soybean sprouts enriched with L. plantarum can be recommended for consumers who prefer new functional foods with pro-health properties.
- Published
- 2020
47. Protection from cytomegalovirus viremia following glycoprotein B vaccination is not dependent on neutralizing antibodies
- Author
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Paul D. Griffiths, Michael Mach, Matthew B. Reeves, Barbara Kropff, Sylvie Pichon, Lyn R. Ambrose, Richard S. B. Milne, Megan McIntosh, Claire Atkinson, Gary R. McLean, and Ilona Baraniak
- Subjects
0301 basic medicine ,Human cytomegalovirus ,viruses ,Cytomegalovirus ,Viremia ,Antibodies, Viral ,03 medical and health sciences ,Cytomegalovirus Vaccines ,0302 clinical medicine ,Antigen ,Adjuvants, Immunologic ,Viral Envelope Proteins ,Commentaries ,medicine ,Humans ,030212 general & internal medicine ,Neutralizing antibody ,Antibody-dependent cell-mediated cytotoxicity ,Multidisciplinary ,biology ,business.industry ,Vaccination ,Antibody titer ,Biological Sciences ,Viral Load ,medicine.disease ,Virology ,Antibodies, Neutralizing ,030104 developmental biology ,Cytomegalovirus Infections ,biology.protein ,business ,Viral load - Abstract
Human cytomegalovirus (HCMV) is an important pathogen in transplant patients and in congenital infection. Previously, we demonstrated that vaccination with a recombinant viral glycoprotein B (gB)/MF59 adjuvant formulation before solid organ transplant reduced viral load parameters post transplant. Reduced posttransplant viremia was directly correlated with antibody titers against gB consistent with a humoral response against gB being important. Here we show that sera from the vaccinated seronegative patients displayed little evidence of a neutralizing antibody response against cell-free HCMV in vitro. Additionally, sera from seronegative vaccine recipients had minimal effect on the replication of a strain of HCMV engineered to be cell-associated in a viral spread assay. Furthermore, although natural infection can induce antibody-dependent cellular cytotoxicity (ADCC) responses, serological analysis of seronegative vaccinees again presented no evidence of a substantial ADCC-promoting antibody response being generated de novo. Finally, analyses for responses against major antigenic domains of gB following vaccination were variable, and their pattern was distinct compared with natural infection. Taken together, these data argue that the protective effect elicited by the gB vaccine is via a mechanism of action in seronegative vaccinees that cannot be explained by neutralization or the induction of ADCC. More generally, these data, which are derived from a human challenge model that demonstrated that the gB vaccine is protective, highlight the need for more sophisticated analyses of new HCMV vaccines over and above the quantification of an ability to induce potent neutralizing antibody responses in vitro.
- Published
- 2018
48. KPC-2-producing Klebsiella pneumoniae ST11 in a Children's Hospital in Poland
- Author
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Marek Gniadkowski, Katarzyna Bojarska, Monika Machulska, Iwona Żak, D. Żabicka, Waleria Hryniewicz, Anna Baraniak, and Iwona Sowa-Sierant
- Subjects
0301 basic medicine ,Microbiology (medical) ,Male ,medicine.medical_specialty ,KPC-2 producing K. pneumoniae ST11 ,lcsh:QH426-470 ,Klebsiella pneumoniae ,030106 microbiology ,lcsh:QR1-502 ,Microbial Sensitivity Tests ,Intensive Care Units, Pediatric ,Applied Microbiology and Biotechnology ,Microbiology ,lcsh:Microbiology ,beta-Lactam Resistance ,beta-Lactamases ,Teaching hospital ,law.invention ,03 medical and health sciences ,carbapenemase ,law ,Internal medicine ,Drug Resistance, Multiple, Bacterial ,polycyclic compounds ,Medicine ,Humans ,Child ,biology ,business.industry ,Resistance pattern ,Infant ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,bacterial infections and mycoses ,Intensive care unit ,Anti-Bacterial Agents ,Bacterial Typing Techniques ,Electrophoresis, Gel, Pulsed-Field ,Klebsiella Infections ,lcsh:Genetics ,KPC-2 producing Klebsiella pneumoniae ,epidemiology ,Female ,Poland ,business - Abstract
Four Klebsiella pneumoniae isolates from children hospitalized over 10 months in an intensive care unit in a children’s teaching hospital in Poland were analyzed. All of the isolates belonged to a single pulsotype and sequence type (ST) 11, and produced the KPC-2 carbapenemase and extended-spectrum β-lactamase (ESBL) CTX-M-15. They were resistant to a variety of antimicrobials, and their β-lactam resistance patterns were typical for KPC producers. This is one of few cases of identification of KPC (or carbapenemase)-producing K. pneumoniae in a pediatric center in Poland.
- Published
- 2018
49. A new species of Rhigognostis (Lepidoptera, Plutellidae) from the Canary Islands
- Author
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Knud Larsen, Wojciech Kubasik, Tomasz Grzegorczyk, and Edward Baraniak
- Subjects
0106 biological sciences ,Canariella ,Rhigognostis ,Insecta ,biology ,Arthropoda ,Ecology ,010607 zoology ,Zoology ,Biodiversity ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,Micro structure ,Lepidoptera genitalia ,Lepidoptera ,Plutellidae ,Animalia ,Animal Science and Zoology ,Taxonomy (biology) ,Ecology, Evolution, Behavior and Systematics ,Taxonomy - Abstract
Rhigognostis canariella sp. nov. is described from the Canary Islands on the basis of five females. The morphological micro structures of the female genitalia are figured. An illustrative diagnostic summary for three allied congeners, R. annulatella(Curtis, 1832), R. kovacsi (Gozmány, 1952) and R. schmaltzella (Zetterstedt, 1839) is provided.
- Published
- 2017
50. NDM-producing Enterobacteriaceae in Poland, 2012–14: inter-regional outbreak ofKlebsiella pneumoniaeST11 and sporadic cases
- Author
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I. Gawryszewska, Anna Baraniak, Radosław Izdebski, D. Żabicka, T. Ozorowski, Marek Gniadkowski, Katarzyna Bojarska, M. Szarata, N. Pewińska, E Literacka, A. Milner, M. Herda, Janusz Fiett, Waleria Hryniewicz, and H. Tomczak
- Subjects
Adult ,DNA, Bacterial ,Male ,0301 basic medicine ,Microbiology (medical) ,Adolescent ,Gene Transfer, Horizontal ,Genotype ,Klebsiella pneumoniae ,030106 microbiology ,Polymerase Chain Reaction ,beta-Lactamases ,Disease Outbreaks ,Microbiology ,03 medical and health sciences ,Plasmid ,Pulsed-field gel electrophoresis ,Humans ,Outpatient clinic ,Pharmacology (medical) ,Typing ,Pharmacology ,biology ,Nucleic Acid Hybridization ,Outbreak ,Sequence Analysis, DNA ,Middle Aged ,biology.organism_classification ,Klebsiella Infections ,New Delhi metallo-beta-lactamase 1 ,Molecular Typing ,Infectious Diseases ,biology.protein ,Multilocus sequence typing ,Female ,Poland ,Plasmids - Abstract
Objectives The objective of this study was to characterize New Delhi metallo-β-lactamase (NDM)-producing Enterobacteriaceae isolates reported in Poland in 2012-14. Methods Representative isolates were typed by PFGE and MLST. NDM and other β-lactamase genes were amplified and sequenced. Plasmids with blaNDM genes were analysed by nuclease S1 plus hybridization profiling, by transfer assays and by PCR-based replicon typing. The blaNDM genetic context was studied by PCR mapping assays. Results Of 374 cases of infection/colonization with NDM-positive Enterobacteriaceae identified in 2012-14, 370 cases in 40 hospitals, 10 outpatient clinics and 1 nursing home were associated with a Klebsiella pneumoniae outbreak with epicentres in Poznan and Warsaw. The outbreak strain of K. pneumoniae ST11 was similar to an isolate from the Czech Republic from 2013. Like the Czech strain, many of the isolates had two blaNDM-1-carrying IncFII- and IncR-type plasmids of variable size, sharing a blaNDM-1-containing segment. The early isolates also produced CTX-M-15 co-encoded by the IncR-type plasmids, and differentiated later by extensive plasmid rearrangements. Four other NDM cases were reported in 2013, three being associated with arrivals from Montenegro, India or Afghanistan. The Indian Escherichia coli ST448 NDM-5 isolate revealed similarity to a recent isolate from Spain, including the blaNDM genetic context observed previously in E. coli strains in Poland and France (of Congolese and Indian origins, respectively). The Afghani Proteus mirabilis was the second isolate of this species with a chromosomal blaNDM-1 location. Conclusions The largest NDM outbreak in a non-endemic country has been observed, being an alarming phenomenon in resistance epidemiology in Poland.
- Published
- 2015
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