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Enterobacteriaceae producing OXA-48-like carbapenemases in Poland, 2013–January 2017
- Source :
- Journal of Antimicrobial Chemotherapy. 73:620-625
- Publication Year :
- 2017
- Publisher :
- Oxford University Press (OUP), 2017.
-
Abstract
- Objectives To analyse OXA-48 (OXA-48/181)-type carbapenemase-producing Enterobacteriaceae reported in Poland from 2013 until January 2017. Methods Bacterial isolates were typed by PFGE and MLST. Genes coding for OXA-48/181 types and other β-lactamases were amplified and sequenced. Mobile elements with blaOXA-48/181-like genes were PCR mapped. blaOXA-48/181-carrying plasmids were characterized by nuclease S1-hybridization profiling, transfer assays and PCR-based replicon typing, while the chromosomal location of the genes was confirmed by the I-CeuI analysis. Results Fifty-four isolates from 52 patients in 20 hospitals (14 cities) were included, in 14 cases having probable foreign origins indicated. The organisms were genetically diverse and represented numerous pandemic clones, including Klebsiella pneumoniae ST395 (n = 23), ST11, ST15 and ST101, Escherichia coli ST38, ST410 and ST648, and Enterobacter cloacae complex ST78. These produced OXA-48 (n = 49), OXA-181 (n = 4) or OXA-232 (n = 1). One of five K. pneumoniae ST395 pulsotypes caused a multicentre outbreak with 18 cases, which significantly contributed to the total number of patients. Depending on the variant, the blaOXA-48/181-like genes were parts of the Tn1999-, Tn2013- or Tn2016-like transposons, with blaOXA-48 found in an ISEcp1-associated module (Tn2016-like) for the first time. Three genotypes, including E. coli ST38, had chromosomal blaOXA-48 genes, while others carried transmissible IncL (∼60 kb; blaOXA-48; n = 30), IncM (∼80-95 kb; blaOXA-48; n = 4), IncX3 (∼50 kb; blaOXA-181; n = 4) or non-typeable (∼90-160 kb; blaOXA-48 or blaOXA-232) plasmids. Conclusions Even though OXA-48/181 producers seem to occur infrequently in Poland, their epidemiology has been marked by various phenomena, namely multiple imports, several limited transmissions plus one larger clonal outbreak, and possible plasmid transfers.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Klebsiella pneumoniae
030106 microbiology
Microbial Sensitivity Tests
Carbapenem-resistant enterobacteriaceae
beta-Lactamases
03 medical and health sciences
Plasmid
Bacterial Proteins
Enterobacteriaceae
Escherichia coli
Humans
Pharmacology (medical)
Typing
Escherichia coli Infections
Pharmacology
Genetics
biology
Escherichia coli Proteins
Enterobacteriaceae Infections
biology.organism_classification
Bacterial Typing Techniques
Klebsiella Infections
Infectious Diseases
Multilocus sequence typing
Poland
Mobile genetic elements
Enterobacter cloacae
Multilocus Sequence Typing
Subjects
Details
- ISSN :
- 14602091 and 03057453
- Volume :
- 73
- Database :
- OpenAIRE
- Journal :
- Journal of Antimicrobial Chemotherapy
- Accession number :
- edsair.doi.dedup.....a23414355677cbfd02c1ad3f9d9aca13