1. Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing
- Author
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Bossé, Janine T, Li, Yanwen, Fernandez Crespo, Roberto, Lacouture, Sonia, Gottschalk, Marcelo, Sárközi, Rita, Fodor, László, Casas Amoribieta, Maria, Angen, Øystein, Nedbalcova, Katerina, Holden, Matthew TG, Maskell, Duncan J, Tucker, Alexander W, Wren, Brendan W, Rycroft, Andrew N, Langford, Paul R, BRaDP1T consortium, Biotechnology and Biological Sciences Research Council (BBSRC), Pfizer Limited (UK), Biotechnology and Biological Sciences Research Council, University of St Andrews. School of Medicine, University of St Andrews. Infection and Global Health Division, University of St Andrews. Biomedical Sciences Research Complex, and University of St Andrews. Infection Group
- Subjects
0301 basic medicine ,Serotype ,Swine ,QH301 Biology ,animal diseases ,Actinobacillus Infections ,Multiplex ,Pathogen ,Swine Diseases ,Genetics ,biology ,Actinobacillus pleuropneumoniae ,Polysaccharides, Bacterial ,QR Microbiology ,General Medicine ,Amplicon ,respiratory system ,3. Good health ,Pleuropneumonia ,Sequence Analysis ,mPCR ,0605 Microbiology ,Sequence analysis ,030106 microbiology ,Serovars ,Serogroup ,Microbiology ,Article ,QH301 ,03 medical and health sciences ,BRaDP1T consortium ,medicine ,Animals ,Typing ,Diagnostic ,Veterinary Sciences ,Serotyping ,Bacterial Capsules ,General Veterinary ,0707 Veterinary Sciences ,A. pleuropneumoniae ,Capsule typing ,DAS ,biology.organism_classification ,medicine.disease ,bacterial infections and mycoses ,respiratory tract diseases ,QR ,030104 developmental biology ,Multiplex Polymerase Chain Reaction - Abstract
Highlights • Analysis of complete capsule loci in all 18 serovars of A. pleuropneumoniae. • Novel insights into evolution of capsule loci in A. pleuropneumoniae. • Development of two mPCRs for comprehensive capsule typing., Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.
- Published
- 2018