Back to Search
Start Over
A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay
- Source :
- JOURNAL OF CLINICAL MICROBIOLOGY, Journal of Clinical Microbiology
- Publication Year :
- 2017
-
Abstract
- Actinobacillus pleuropneumoniae causes pleuropneumonia, an economically significant lung disease of pigs. Recently, isolates of A. pleuropneumoniae that were serologically distinct from the previously characterized 15 serovars were described, and a proposal was put forward that they comprised a new serovar, serovar 16. Here we used whole-genome sequencing of the proposed serovar 16 reference strain A-85/14 to confirm the presence of a unique capsular polysaccharide biosynthetic locus. For molecular diagnostics, primers were designed from the capsule locus of strain A-85/14, and a PCR was formulated that differentiated serovar 16 isolates from all 15 known serovars and other common respiratory pathogenic/commensal bacteria of pigs. Analysis of the capsule locus of strain A-85/14 combined with the previous serological data show the existence of a sixteenth serovar—designated serovar 16—of A. pleuropneumoniae.
- Subjects :
- DNA, Bacterial
STRUCTURAL-CHARACTERIZATION
Swine
Serogroup
Polymerase Chain Reaction
Microbiology
SEQUENCE
Clinical Veterinary Microbiology
Actinobacillus Infections
diagnostics
Animals
LIPOPOLYSACCHARIDE-O-CHAIN
Bacterial Capsules
DNA Primers
Swine Diseases
Pleuropneumonia
Science & Technology
STRAINS
Actinobacillus pleuropneumoniae
Sequence Analysis, DNA
11 Medical And Health Sciences
06 Biological Sciences
respiratory system
serovar 16
PCR
POLYSACCHARIDE
Molecular Diagnostic Techniques
Genetic Loci
07 Agricultural And Veterinary Sciences
Life Sciences & Biomedicine
MULTIPLEX PCR
Genome, Bacterial
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- JOURNAL OF CLINICAL MICROBIOLOGY, Journal of Clinical Microbiology
- Accession number :
- edsair.pmid.dedup....bba11171bfd8bcd9b8af51834e531b4e