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Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing
- Source :
- Veterinary Microbiology, VETERINARY MICROBIOLOGY
- Publication Year :
- 2018
- Publisher :
- Elsevier, 2018.
-
Abstract
- Highlights • Analysis of complete capsule loci in all 18 serovars of A. pleuropneumoniae. • Novel insights into evolution of capsule loci in A. pleuropneumoniae. • Development of two mPCRs for comprehensive capsule typing.<br />Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.
- Subjects :
- 0301 basic medicine
Serotype
Swine
QH301 Biology
animal diseases
Actinobacillus Infections
Multiplex
Pathogen
Swine Diseases
Genetics
biology
Actinobacillus pleuropneumoniae
Polysaccharides, Bacterial
QR Microbiology
General Medicine
Amplicon
respiratory system
3. Good health
Pleuropneumonia
Sequence Analysis
mPCR
0605 Microbiology
Sequence analysis
030106 microbiology
Serovars
Serogroup
Microbiology
Article
QH301
03 medical and health sciences
BRaDP1T consortium
medicine
Animals
Typing
Diagnostic
Veterinary Sciences
Serotyping
Bacterial Capsules
General Veterinary
0707 Veterinary Sciences
A. pleuropneumoniae
Capsule typing
DAS
biology.organism_classification
medicine.disease
bacterial infections and mycoses
respiratory tract diseases
QR
030104 developmental biology
Multiplex Polymerase Chain Reaction
Subjects
Details
- ISSN :
- 03781135
- Database :
- OpenAIRE
- Journal :
- Veterinary Microbiology, VETERINARY MICROBIOLOGY
- Accession number :
- edsair.doi.dedup.....29b3b58a7a286681a436e09f471ee130