1. Correlations between camptothecin and related metabolites in Camptotheca acuminata reveal similar biosynthetic principles and in planta synergistic effects
- Author
-
Kusari, Souvik, Zühlke, Sebastian, and Spiteller, Michael
- Subjects
- *
ALGORITHMS , *ALTERNATIVE medicine , *ANALYSIS of variance , *ANTINEOPLASTIC agents , *CLUSTER analysis (Statistics) , *COMPARATIVE studies , *COMPUTER software , *DISCRIMINANT analysis , *FACTOR analysis , *LIQUID chromatography , *MASS spectrometry , *MEDICINAL plants , *MULTIVARIATE analysis , *POPULATION geography , *RESEARCH funding , *PLANT stems , *PHYTOCHEMICALS , *PLANT extracts , *DATA analysis - Abstract
Abstract: Camptothecin (CPT), 9-methoxycamptothecin (9-MeO-CPT), and 10-hydroxycamptothecin (10-OH-CPT) are potent antineoplastic metabolites. We analyzed these metabolites in Camptotheca acuminata sampled from Germany and China, using LC–MS/MS and LC-ESI–HRMS/MS, coupled with chemometrics. Multivariate analysis revealed that fresh stems of C. acuminata from China had the highest comprehensive metabolite load. Significant positive correlations of CPT with 9-MeO-CPT and 10-OH-CPT were observed by Kruskal''s multidimensional scaling and principal component analysis. Linear discriminant analysis and hierarchical agglomerative cluster analysis revealed that C. acuminata from China was separated from others. These positive correlations indicate that these metabolites are biosynthesized similarly and operate synergistically in planta. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
- View/download PDF