1. Sensory input drives rapid homeostatic scaling of the axon initial segment in mouse barrel cortex
- Author
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Jamann, Nora, Dannehl, Dominik, Lehmann, Nadja, Wagener, Robin, Thielemann, Corinna, Schultz, Christian, Staiger, Jochen, Kole, Maarten H.P., Engelhardt, Maren, Celbiologie, Sub Cell Biology, Netherlands Institute for Neuroscience (NIN), Celbiologie, and Sub Cell Biology
- Subjects
0301 basic medicine ,Aging ,Time Factors ,Chemistry(all) ,Science ,General Physics and Astronomy ,Sensory system ,Physics and Astronomy(all) ,Biology ,Biochemistry ,Article ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Animals ,Homeostasis ,Premovement neuronal activity ,Sensory deprivation ,Axon Initial Segment ,Action potential initiation ,Cerebral Cortex ,Neuronal Plasticity ,Multidisciplinary ,Biochemistry, Genetics and Molecular Biology(all) ,Pyramidal Cells ,General Chemistry ,Barrel cortex ,Axon initial segment ,Mice, Inbred C57BL ,Electrophysiology ,030104 developmental biology ,Vibrissae ,Somatosensory system ,Exploratory Behavior ,Sensory Deprivation ,Neuroscience ,030217 neurology & neurosurgery ,Genetics and Molecular Biology(all) - Abstract
The axon initial segment (AIS) is a critical microdomain for action potential initiation and implicated in the regulation of neuronal excitability during activity-dependent plasticity. While structural AIS plasticity has been suggested to fine-tune neuronal activity when network states change, whether it acts in vivo as a homeostatic regulatory mechanism in behaviorally relevant contexts remains poorly understood. Using the mouse whisker-to-barrel pathway as a model system in combination with immunofluorescence, confocal analysis and electrophysiological recordings, we observed bidirectional AIS plasticity in cortical pyramidal neurons. Furthermore, we find that structural and functional AIS remodeling occurs in distinct temporal domains: Long-term sensory deprivation elicits an AIS length increase, accompanied with an increase in neuronal excitability, while sensory enrichment results in a rapid AIS shortening, accompanied by a decrease in action potential generation. Our findings highlight a central role of the AIS in the homeostatic regulation of neuronal input-output relations., The axon initial segment (AIS) is critical for action potential initiation and implicated in the regulation of neuronal excitability. The authors describe bidirectional AIS plasticity in a behaviourally relevant context, revealing that the AIS acts in vivo as a homeostatic regulatory domain.
- Published
- 2021