1. Impact of liquid hog manure applications on antibiotic resistance genes concentration in soil and drainage water in field crops
- Author
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Marie-Ève Tremblay, Marc-Olivier Gasser, Mohamed Rhouma, Mylène Généreux, Élodie Larouche, Caroline Côté, and Sylvain Quessy
- Subjects
0301 basic medicine ,Swine ,030106 microbiology ,Immunology ,Applied Microbiology and Biotechnology ,Microbiology ,03 medical and health sciences ,Drug Resistance, Bacterial ,Genetics ,Animals ,Drainage ,Molecular Biology ,Soil Microbiology ,Bacteria ,business.industry ,Agriculture ,General Medicine ,Manure ,Anti-Bacterial Agents ,030104 developmental biology ,Agronomy ,Genes, Bacterial ,Environmental science ,Water Microbiology ,business ,Antibiotic resistance genes - Abstract
Agricultural practices such as manure applications could contribute to the spread of antibiotic resistance genes (ARGs) within the environment. Our objective was to assess the impact of certain fertilization methods (mineral or manure) and tillage practices (reduced or conventional) on the presence of ARGs and bacteria in soil and drainage water under wheat and grain corn crops. Targeted ARGs tet(T), sul1, and blaCTX-M-1 in liquid hog manure, soil, and water samples were quantified by qPCR. Conventional PCR was used to detect mcr-1 and mcr-2. ARGs in control plots were detected despite the absence of manure, representing an environmental reservoir of resistant microorganisms. The manure application rate higher than 39 m3/ha increased tet(T) and sul1 gene concentrations in soil for more than 180 days. Tillage practices had no impact on ARG concentrations in soil and water samples. The blaCTX-M-1 gene was only detected in seven water samples in 2016, but no link was established with the treatments. The mcr-1 and mcr-2 genes were not detected in all tested samples. This study demonstrated that tet(T) and sul1 gene concentrations increased in soil after liquid hog manure application as well as in drainage water in the next weeks.
- Published
- 2020