Back to Search
Start Over
Prevalence of colistin resistance and mcr-1/mcr-2 genes in extended-spectrum β-lactamase/AmpC-producing Escherichia coli isolated from chickens in Canada, Senegal and Vietnam
- Source :
- Journal of global antimicrobial resistance. 19
- Publication Year :
- 2018
-
Abstract
- Objectives This study investigated the prevalence of Escherichia coli (E. coli) colistin resistance and mcr-1 and mcr-2 genes among extended-spectrum β-lactamase (ESBL)/AmpC-producing E. coli isolates recovered from chicken feces in Canada (Quebec), Senegal and Vietnam, and evaluated the susceptibility pattern of the colistin-resistant E. coli isolates to other clinically relevant antimicrobials. Methods A total of 327 potential ESBL/AmpC-producing E. coli isolates from chicken farms in Canada (Quebec), Senegal and Vietnam were analysed for colistin susceptibility by broth microdilution method and for the presence of mcr (1-2) genes by PCR. The pmrA and pmrB genes of colistin-resistant E. coli isolates, in the absence of mcr (1-2) genes, were sequenced. Antimicrobial resistance phenotypes of colistin-resistant E. coli isolates were determined by disk diffusion. Results None of the 108 potential ESBL/AmpC-producing E. coli isolates from seven farms in Canada were colistin-resistant or possessed mcr-1 or mcr-2 gene. A low prevalence of 2.2% of colistin resistance was observed in 93 Senegalese isolates from the 15 sampled farms, although neither mcr-1 nor mcr-2 gene was found. A prevalence of 8.7% of colistin resistance was observed among 126 Vietnamese isolates from two of the four sampled farms. The mcr-1 gene was detected in 85% of the 13 phenotypically colistin-resistant isolates. Moreover, all colistin-resistant isolates presented a multidrug-resistant phenotype. Conclusions The co-existence of the mcr-1 and ESBL/AmpC genes and the very high level of multiple drug resistance in all colistin-resistant E. coli isolates obtained from sampled chicken farms in Vietnam is a major concern.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Farms
030106 microbiology
Immunology
Microbial Sensitivity Tests
Biology
medicine.disease_cause
Microbiology
beta-Lactamases
03 medical and health sciences
Feces
0302 clinical medicine
Antibiotic resistance
Bacterial Proteins
Drug Resistance, Multiple, Bacterial
polycyclic compounds
medicine
Escherichia coli
Prevalence
Immunology and Allergy
Animals
030212 general & internal medicine
Escherichia coli Infections
Poultry Diseases
Colistin
Escherichia coli Proteins
Broth microdilution
Quebec
Membrane Proteins
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
Antimicrobial
Senegal
3. Good health
Anti-Bacterial Agents
Multiple drug resistance
Vietnam
bacteria
MCR-1
Chickens
hormones, hormone substitutes, and hormone antagonists
medicine.drug
Subjects
Details
- ISSN :
- 22137173
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Journal of global antimicrobial resistance
- Accession number :
- edsair.doi.dedup.....3975d94eaa64cb0532815e12c2ea5d48