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In vivo therapeutic efficacy and pharmacokinetics of colistin sulfate in an experimental model of enterotoxigenic Escherichia coli infection in weaned pigs
- Source :
- Veterinary Research, Veterinary Research, BioMed Central, 2016, 47 (1), pp.58. ⟨10.1186/s13567-016-0344-y⟩
- Publisher :
- Springer Nature
-
Abstract
- Enterotoxigenic Escherichia coli (ETEC: F4) associated with post-weaning diarrhea (PWD) in pigs has developed resistance against several antimicrobial families, leading to increased use of colistin sulfate (CS) for the treatment of this disease. The objective of this study was to determine the efficacy of oral CS treatment in experimental PWD due to ETEC: F4 challenge and determine the effect of this challenge on CS intestinal absorption. In this study, 96 pigs were divided into two trials based on CS dose (100 000 or 50 000 IU/kg). Fecal shedding of ETEC: F4, total E. coli, and CS-resistant E. coli, diarrhea scores, and weight changes were evaluated. Colistin sulfate plasma concentrations were determined by HPLC–MS/MS. Regardless of the dose, CS treatment resulted in a reduction of fecal ETEC: F4 and total E. coli shedding, and in diarrhea scores but only during the treatment period. However, CS treatment resulted in a slight increase in fecal shedding of CS resistant E. coli and did not prevent weight loss in challenged pigs. In addition, challenge with ETEC: F4 resulted in an increase of CS intestinal absorption. Our study is among the first to demonstrate that under controlled conditions, CS was effective in reducing fecal shedding of ETEC: F4 and total E. coli in experimental PWD. However, CS treatment was associated with a slight selection pressure on E. coli and did not prevent pig weight loss. Further studies are needed in field conditions, to better characterize CS therapeutic regimen efficacy and bacterial resistance dissemination. Electronic supplementary material The online version of this article (doi:10.1186/s13567-016-0344-y) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Swine
[SDV]Life Sciences [q-bio]
030106 microbiology
Porcine Reproductive and Respiratory Syndrome
Administration, Oral
Biology
medicine.disease_cause
Intestinal absorption
Microbiology
03 medical and health sciences
Feces
Antibiotic resistance
Pharmacokinetics
In vivo
Enterotoxigenic Escherichia coli
medicine
Animals
Escherichia coli Infections
2. Zero hunger
Bacterial Shedding
General Veterinary
Colistin
Antimicrobial
veterinary(all)
3. Good health
Anti-Bacterial Agents
Diarrhea
Disease Models, Animal
030104 developmental biology
Intestinal Absorption
medicine.symptom
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 12979716 and 09284249
- Volume :
- 47
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Veterinary Research
- Accession number :
- edsair.doi.dedup.....a7e6d3fa483d618d9f27ebc45713b75e
- Full Text :
- https://doi.org/10.1186/s13567-016-0344-y