1. BPA disrupts 17-estradiol-mediated hepatic protection against ischemia/reperfusion injury in rat liver by upregulating the Ang II/AT1R signaling pathway
- Author
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Zeyu Li, Yili Zhang, Yu Shi, Mei Zhang, Liang Yu, Liankang Sun, and Sheng-Li Wu
- Subjects
0301 basic medicine ,Male ,Cancer Research ,bisphenol A ,Endocrine Disruptors ,Biochemistry ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,0302 clinical medicine ,estrogen ,rat ,biology ,Estradiol ,Chemistry ,Angiotensin II ,Articles ,Up-Regulation ,Losartan ,Xenoestrogen ,Oncology ,Endocrine disruptor ,Liver ,030220 oncology & carcinogenesis ,Reperfusion Injury ,Molecular Medicine ,Alkaline phosphatase ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug ,Signal Transduction ,medicine.medical_specialty ,endocrine system ,ischemia-reperfusion injury ,Protective Agents ,Receptor, Angiotensin, Type 1 ,03 medical and health sciences ,Phenols ,Internal medicine ,Genetics ,medicine ,Animals ,Benzhydryl Compounds ,Molecular Biology ,urogenital system ,Estrogens ,medicine.disease ,Rats ,030104 developmental biology ,Endocrinology ,Alanine transaminase ,biology.protein ,Liver function ,Reperfusion injury - Abstract
Bisphenol A (BPA), a xenoestrogen commonly used in plastics, may act as an endocrine disruptor, which indicates that BPA might be a public health risk. The present study aimed to investigate the effect of BPA on 17β‑estradiol (E2)‑mediated protection against liver ischemia/reperfusion (I/R) injury, and to identify the underlying mechanisms using a rat model. A total of 56 male Sprague Dawley rats were randomly divided into the following seven groups: i) Sham; ii) I/R; iii) Sham + BPA; iv) I/R + BPA; v) I/R + E2; vi) I/R + E2 + BPA; and vii) I/R + E2 + BPA + losartan [LOS; an angiotensin II (Ang II) type I receptor (ATIR) antagonist]. A rat model of hepatic I/R injury was established by inducing hepatic ischemia for 60 min followed by reperfusion for 24 h. When ischemia was induced, rats were treated with vehicle, E2, BPA or LOS. After 24 h of reperfusion, blood samples and hepatic tissues were collected for histopathological and biochemical examinations. The results suggested that 4 mg/kg BPA did not significantly alter the liver function, or Ang II and AT1R expression levels in the Sham and I/R groups. However, 4 mg/kg BPA inhibited E2‑mediated hepatic protection by enhancing hepatic necrosis, and increasing the release of alanine transaminase, alkaline phosphatase and total bilirubin (P
- Published
- 2020