Back to Search
Start Over
Resveratrol-Induced Downregulation of NAF-1 Enhances the Sensitivity of Pancreatic Cancer Cells to Gemcitabine via the ROS/Nrf2 Signaling Pathways
- Source :
- Oxidative Medicine and Cellular Longevity, Vol 2018 (2018), Oxidative Medicine and Cellular Longevity
- Publication Year :
- 2018
- Publisher :
- Hindawi, 2018.
-
Abstract
- NAF-1 (nutrient-deprivation autophagy factor-1), which is an outer mitochondrial membrane protein, is known to play important roles in calcium metabolism, antiapoptosis, and antiautophagy. Resveratrol, a natural polyphenolic compound, is considered as a potent anticancer agent. Nevertheless, the molecular mechanisms underlying the effects of resveratrol and NAF-1 and their mediation of drug resistance in pancreatic cancer remain unclear. Here, we demonstrate that resveratrol suppresses the expression of NAF-1 in pancreatic cancer cells by inducing cellular reactive oxygen species (ROS) accumulation and activating Nrf2 signaling. In addition, the knockdown of NAF-1 activates apoptosis and impedes the proliferation of pancreatic cancer cells. More importantly, the targeting of NAF-1 by resveratrol can improve the sensitivity of pancreatic cancer cells to gemcitabine. These results highlight the significance of strategies that target NAF-1, which may enhance the efficacy of gemcitabine in pancreatic cancer therapy.
- Subjects :
- 0301 basic medicine
Aging
Apoptosis
Resveratrol
Biochemistry
Deoxycytidine
chemistry.chemical_compound
0302 clinical medicine
Stilbenes
RNA, Small Interfering
chemistry.chemical_classification
Gene knockdown
Chemistry
lcsh:Cytology
food and beverages
General Medicine
Mitochondria
Proto-Oncogene Proteins c-bcl-2
Ribonucleoproteins
030220 oncology & carcinogenesis
RNA Interference
medicine.drug
Signal Transduction
Research Article
inorganic chemicals
Article Subject
NF-E2-Related Factor 2
Down-Regulation
03 medical and health sciences
Downregulation and upregulation
Pancreatic cancer
Cell Line, Tumor
medicine
Humans
lcsh:QH573-671
Cell Proliferation
Reactive oxygen species
Autophagy
technology, industry, and agriculture
Cell Biology
medicine.disease
Gemcitabine
Pancreatic Neoplasms
body regions
030104 developmental biology
Cancer research
Reactive Oxygen Species
Subjects
Details
- Language :
- English
- ISSN :
- 19420900
- Database :
- OpenAIRE
- Journal :
- Oxidative Medicine and Cellular Longevity
- Accession number :
- edsair.doi.dedup.....2ec51c4a91c1a9004f4fcad17477aa24
- Full Text :
- https://doi.org/10.1155/2018/9482018