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miR-1204 promotes hepatocellular carcinoma progression through activating MAPK and c-Jun/AP1 signaling by targeting ZNF418
- Source :
- International Journal of Biological Sciences
- Publication Year :
- 2019
- Publisher :
- Ivyspring International Publisher, 2019.
-
Abstract
- Emerging evidence has indicated that abnormal microRNAs (miRNAs) participated in carcinogenesis and tumor progression in hepatocellular carcinoma (HCC). Better understanding the association between miRNAs and HCC may contribute to discover novel therapeutic approaches for diagnosis and treatments. In the current study, we have shown that miR-1204 level was elevated in HCC tissues and cell lines, which was associated with malignant clinical features, including large tumor size and advanced TNM stage. Furthermore, gain-or loss-of function assays demonstrated that miR-1204 promoted cell proliferation in vitro and tumor growth in vivo as well as inhibited apoptosis in vitro. Luciferase reporter gene assays confirmed that ZNF418 was a direct downstream target of miR-1204. Recuse assays showed that ZNF418 mediates the biological function of miR-1204 on HCC cells through regulating MAPK and c-Jun signaling. In conclusion, our results suggest that miR-1204 functions as an oncogene to promote proliferation and inhibit apoptosis through regulating MAPK and c-Jun signaling by targeting ZNF418, and potentially serves as a novel prognostic biomarker and therapeutic target for HCC.
- Subjects :
- Male
MAPK/ERK pathway
Carcinogenesis
Proto-Oncogene Proteins c-jun
Apoptosis
medicine.disease_cause
Applied Microbiology and Biotechnology
Mice
Cell Movement
miR-1204
3' Untranslated Regions
Mice, Inbred BALB C
0303 health sciences
Liver Neoplasms
c-jun
hepatocellular carcinoma
Middle Aged
Prognosis
AP-1 transcription factor
Disease Progression
Female
Signal Transduction
Research Paper
Adult
Carcinoma, Hepatocellular
Cell Survival
MAP Kinase Signaling System
ZNF418
Mice, Nude
Biology
03 medical and health sciences
Cell Line, Tumor
microRNA
Biomarkers, Tumor
medicine
Animals
Humans
Molecular Biology
Ecology, Evolution, Behavior and Systematics
Aged
Cell Proliferation
030304 developmental biology
Oncogene
Cell growth
c-Jun
Cell Biology
MAPK
digestive system diseases
Repressor Proteins
MicroRNAs
Tumor progression
Cancer research
Neoplasm Transplantation
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 14492288
- Volume :
- 15
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Sciences
- Accession number :
- edsair.doi.dedup.....3b638a6053e81f0cc46720960f0249f1