1. pH Sensitive Dextran Coated Fluorescent Nanodiamonds as a Biomarker for HeLa Cells Endocytic Pathway and Increased Cellular Uptake
- Author
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Linyan Nie, Romana Schirhagl, Lei Li, Yue Zhang, Patrick van Rijn, Nanotechnology and Biophysics in Medicine (NANOBIOMED), Restoring Organ Function by Means of Regenerative Medicine (REGENERATE), and Basic and Translational Research and Imaging Methodology Development in Groningen (BRIDGE)
- Subjects
General Chemical Engineering ,Endocytic cycle ,PROTEIN ,Endocytosis Pathway ,02 engineering and technology ,Article ,HeLa ,03 medical and health sciences ,chemistry.chemical_compound ,endocytic pathway ,LYSOSOME ,Lysosome ,medicine ,NANOPARTICLES ,General Materials Science ,DRUG-DELIVERY ,Nanodiamond ,QD1-999 ,030304 developmental biology ,0303 health sciences ,biology ,Chemistry ,cellular uptake ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Fluorescence ,DYSFUNCTION ,medicine.anatomical_structure ,Dextran ,nanodiamonds ,Biophysics ,0210 nano-technology ,Intracellular ,ACIDIFICATION - Abstract
Fluorescent nanodiamonds are a useful for biosensing of intracellular signaling networks or environmental changes (such as temperature, pH or free radical generation). HeLa cells are interesting to study with these nanodiamonds since they are a model cell system that is widely used to study cancer-related diseases. However, they only internalize low numbers of nanodiamond particles very slowly via the endocytosis pathway. In this work, we show that pH-sensitive, dextran-coated fluorescent nanodiamonds can be used to visualise this pathway. Additionally, this coating improved diamond uptake in HeLa cells by 5.3 times (*** p <, 0.0001) and decreased the required time for uptake to only 30 min. We demonstrated further that nanodiamonds enter HeLa cells via endolysosomes and are eventually expelled by cells.
- Published
- 2021