30 results on '"Iskar, Murat"'
Search Results
2. Integration of transcriptomics, proteomics and loss-of-function screening reveals WEE1 as a target for combination with dasatinib against proneural glioblastoma
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Alhalabi, Obada T., Göttmann, Mona, Gold, Maxwell P., Schlue, Silja, Hielscher, Thomas, Iskar, Murat, Kessler, Tobias, Hai, Ling, Lokumcu, Tolga, Cousins, Clara C., Herold-Mende, Christel, Heßling, Bernd, Horschitz, Sandra, Jabali, Ammar, Koch, Philipp, Baumgartner, Ulrich, Day, Bryan W., Wick, Wolfgang, Sahm, Felix, Krieg, Sandro M., Fraenkel, Ernest, Phillips, Emma, and Goidts, Violaine
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- 2024
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3. Readout of histone methylation by Trim24 locally restricts chromatin opening by p53
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Isbel, Luke, Iskar, Murat, Durdu, Sevi, Weiss, Joscha, Grand, Ralph S., Hietter-Pfeiffer, Eric, Kozicka, Zuzanna, Michael, Alicia K., Burger, Lukas, Thomä, Nicolas H., and Schübeler, Dirk
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- 2023
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4. Proteogenomics refines the molecular classification of chronic lymphocytic leukemia
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Herbst, Sophie A., Vesterlund, Mattias, Helmboldt, Alexander J., Jafari, Rozbeh, Siavelis, Ioannis, Stahl, Matthias, Schitter, Eva C., Liebers, Nora, Brinkmann, Berit J., Czernilofsky, Felix, Roider, Tobias, Bruch, Peter-Martin, Iskar, Murat, Kittai, Adam, Huang, Ying, Lu, Junyan, Richter, Sarah, Mermelekas, Georgios, Umer, Husen Muhammad, Knoll, Mareike, Kolb, Carolin, Lenze, Angela, Cao, Xiaofang, Österholm, Cecilia, Wahnschaffe, Linus, Herling, Carmen, Scheinost, Sebastian, Ganzinger, Matthias, Mansouri, Larry, Kriegsmann, Katharina, Kriegsmann, Mark, Anders, Simon, Zapatka, Marc, Del Poeta, Giovanni, Zucchetto, Antonella, Bomben, Riccardo, Gattei, Valter, Dreger, Peter, Woyach, Jennifer, Herling, Marco, Müller-Tidow, Carsten, Rosenquist, Richard, Stilgenbauer, Stephan, Zenz, Thorsten, Huber, Wolfgang, Tausch, Eugen, Lehtiö, Janne, and Dietrich, Sascha
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- 2022
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5. Quantitative Proteome Landscape of the NCI-60 Cancer Cell Lines
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Guo, Tiannan, Luna, Augustin, Rajapakse, Vinodh N., Koh, Ching Chiek, Wu, Zhicheng, Liu, Wei, Sun, Yaoting, Gao, Huanhuan, Menden, Michael P., Xu, Chao, Calzone, Laurence, Martignetti, Loredana, Auwerx, Chiara, Buljan, Marija, Banaei-Esfahani, Amir, Ori, Alessandro, Iskar, Murat, Gillet, Ludovic, Bi, Ran, Zhang, Jiangnan, Zhang, Huanhuan, Yu, Chenhuan, Zhong, Qing, Varma, Sudhir, Schmitt, Uwe, Qiu, Peng, Zhang, Qiushi, Zhu, Yi, Wild, Peter J., Garnett, Mathew J., Bork, Peer, Beck, Martin, Liu, Kexin, Saez-Rodriguez, Julio, Elloumi, Fathi, Reinhold, William C., Sander, Chris, Pommier, Yves, and Aebersold, Ruedi
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- 2019
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6. The landscape of viral associations in human cancers
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Zapatka, Marc, Borozan, Ivan, Brewer, Daniel S., Iskar, Murat, Grundhoff, Adam, Alawi, Malik, Desai, Nikita, Sültmann, Holger, Moch, Holger, Cooper, Colin S., Eils, Roland, Ferretti, Vincent, and Lichter, Peter
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- 2020
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7. Molecular subgrouping of primary pineal parenchymal tumors reveals distinct subtypes correlated with clinical parameters and genetic alterations
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Pfaff, Elke, Aichmüller, Christian, Sill, Martin, Stichel, Damian, Snuderl, Matija, Karajannis, Matthias A., Schuhmann, Martin U., Schittenhelm, Jens, Hasselblatt, Martin, Thomas, Christian, Korshunov, Andrey, Rhizova, Marina, Wittmann, Andrea, Kaufhold, Anna, Iskar, Murat, Ketteler, Petra, Lohmann, Dietmar, Orr, Brent A., Ellison, David W., von Hoff, Katja, Mynarek, Martin, Rutkowski, Stefan, Sahm, Felix, von Deimling, Andreas, Lichter, Peter, Kool, Marcel, Zapatka, Marc, Pfister, Stefan M., and Jones, David T. W.
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- 2020
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8. Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.
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Lokumcu, Tolga, Iskar, Murat, Schneider, Martin, Helm, Dominic, Klinke, Glynis, Schlicker, Lisa, Bethke, Frederic, Müller, Gabriele, Richter, Karsten, Poschet, Gernot, Phillips, Emma, and Goidts, Violaine
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- 2024
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9. Luminal signalling links cell communication to tissue architecture during organogenesis
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Durdu, Sevi, Iskar, Murat, Revenu, Celine, Schieber, Nicole, Kunze, Andreas, Bork, Peer, Schwab, Yannick, and Gilmour, Darren
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Embryonic stem cells -- Physiological aspects -- Genetic aspects -- Research ,Cellular signal transduction -- Physiological aspects -- Genetic aspects -- Research ,Environmental issues ,Science and technology ,Zoology and wildlife conservation - Abstract
Morphogenesis is the process whereby cell collectives are shaped into differentiated tissues and organs (1). The self-organizing nature of morphogenesis has been recently demonstrated by studies showing that stem cells in three-dimensional culture can generate complex organoids, such as mini-guts (2), optic-cups (3) and even mini-brains (4). To achieve this, cell collectives must regulate the activity of secreted signalling molecules that control cell differentiation, presumably through the self-assembly of microenvironments or niches. However, mechanisms that allow changes in tissue architecture to feedback directly on the activity of extracellular signals have not been described. Here we investigate how the process of tissue assembly controls signalling activity during organogenesis in vivo, using the migrating zebrafish lateral line primordium (5). We show that fibroblast growth factor (FGF) activity within the tissue controls the frequency at which it deposits rosette-like mechanosensory organs. Live imaging reveals that FGF becomes specifically concentrated in microluminal structures that assemble at the centre of these organs and spatially constrain its signalling activity. Genetic inhibition of microlumen assembly and laser micropuncture experiments demonstrate that microlumina increase signalling responses in participating cells, thus allowing FGF to coordinate the migratory behaviour of cell groups at the tissue rear. As the formation of a central lumen is a self-organizing property of many cell types, such as epithelia (6) and embryonic stem cells (7), luminal signalling provides a potentially general mechanism to locally restrict, coordinate and enhance cell communication within tissues., A major challenge in biology is to explain how the pattern of complex organs emerges through dynamic self-organizing processes occurring at cellular and molecular scales (1,8,9). The development of the [...]
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- 2014
10. A synergistic interaction between HDAC‐ and PARP inhibitors in childhood tumors with chromothripsis.
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Khalid, Umar, Simovic, Milena, Hammann, Linda A., Iskar, Murat, Wong, John K. L., Kumar, Rithu, Jugold, Manfred, Sill, Martin, Bolkestein, Michiel, Kolb, Thorsten, Hergt, Michaela, Devens, Frauke, Ecker, Jonas, Kool, Marcel, Milde, Till, Westermann, Frank, Benner, Axel, Lewis, Joe, Dietrich, Sascha, and Pfister, Stefan M.
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POLY(ADP-ribose) polymerase ,RECOMBINANT DNA ,DOUBLE-strand DNA breaks ,NEUROBLASTOMA ,DNA repair ,HISTONE deacetylase inhibitors - Abstract
Chromothripsis is a form of genomic instability characterized by the occurrence of tens to hundreds of clustered DNA double‐strand breaks in a one‐off catastrophic event. Rearrangements associated with chromothripsis are detectable in numerous tumor entities and linked with poor prognosis in some of these, such as Sonic Hedgehog medulloblastoma, neuroblastoma and osteosarcoma. Hence, there is a need for therapeutic strategies eliminating tumor cells with chromothripsis. Defects in DNA double‐strand break repair, and in particular homologous recombination repair, have been linked with chromothripsis. Targeting DNA repair deficiencies by synthetic lethality approaches, we performed a synergy screen using drug libraries (n = 375 compounds, 15 models) combined with either a PARP inhibitor or cisplatin. This revealed a synergistic interaction between the HDAC inhibitor romidepsin and PARP inhibition. Functional assays, transcriptome analyses and in vivo validation in patient‐derived xenograft mouse models confirmed the efficacy of the combinatorial treatment. [ABSTRACT FROM AUTHOR]
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- 2022
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11. DvD: An R/Cytoscape pipeline for drug repurposing using public repositories of gene expression data
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Pacini, Clare, Iorio, Francesco, Gonçalves, Emanuel, Iskar, Murat, Klabunde, Thomas, Bork, Peer, and Saez-Rodriguez, Julio
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- 2013
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12. Pilocytic astrocytoma demethylation and transcriptional landscapes link bZIP transcription factors to immune response.
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Aichmüller, Christian F, Iskar, Murat, Jones, David T W, Korshunov, Andrey, Radlwimmer, Bernhard, Kool, Marcel, Ernst, Aurelie, Pfister, Stefan M, Lichter, Peter, and Zapatka, Marc
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- 2020
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13. CD8+ T-cells of CLL-bearing mice acquire a transcriptional program of T-cell activation and exhaustion.
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Llaó Cid, Laura, Hanna, Bola S., Iskar, Murat, Roessner, Philipp M., Öztürk, Selcen, Lichter, Peter, Zapatka, Marc, and Seiffert, Martina
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T cells ,CHRONIC lymphocytic leukemia ,PROGRAMMED cell death 1 receptors ,CELL physiology ,CYTOTOXIC T cells ,ANIMAL models of cancer ,LABORATORY mice - Abstract
Chronic lymphocytic leukemia (CLL) is associated with an accumulation of oligoclonal CD8
+ effector T-cells, which control leukemia progression in mice, but gradually acquire a dysfunctional phenotype along with disease progression. Exhaustion of CD8+ T-cells is characterized by increased expression of inhibitory receptors like PD-1, decreased proliferation, and reduced effector function such as cytokine production, which reduces anti-tumor control. Despite the accumulation of exhausted PD-1+ CD8+ T-cells in secondary lymphoid organs of CLL patients, immune checkpoint blockade as a means to reinvigorate anti-tumor T-cell activity has not shown the expected efficacy. This highlights the need for a better understanding of T-cell exhaustion in CLL. Here, we uncover the transcriptional program of T-cell exhaustion in CLL by comparing naïve with dysfunctional effector CD8+ T-cells with high PD-1 expression from mice after adoptive transfer of Eµ-TCL1 leukemic cells. Our data provide clear evidence for activation-induced dysfunction of CD8+ T-cells in the CLL microenvironment and assess the heterogeneity in the expression of functionally relevant proteins in specific clusters of CD8+ T-cells at a single-cell level. [ABSTRACT FROM AUTHOR]- Published
- 2020
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14. DECIPHER pooled shRNA library screen identifies PP2A and FGFR signaling as potential therapeutic targets for diffuse intrinsic pontine gliomas.
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Schramm, Kathrin, Iskar, Murat, Statz, Britta, Jäger, Natalie, Haag, Daniel, Słabicki, Mikołaj, Pfister, Stefan M, Zapatka, Marc, Gronych, Jan, Jones, David T W, and Lichter, Peter
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- 2019
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15. Tumor-derived exosomes modulate PD-L1 expression in monocytes.
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Haderk, Franziska, Schulz, Ralph, Iskar, Murat, Cid, Laura Llaó, Worst, Thomas, Willmund, Karolin V., Schulz, Angela, Warnken, Uwe, Seiler, Jana, Benner, Axel, Nessling, Michelle, Zenz, Thorsten, Göbel, Maria, Dürig, Jan, Diederichs, Sven, Paggetti, Jérôme, Moussay, Etienne, Stilgenbauer, Stephan, Zapatka, Marc, and Lichter, Peter
- Abstract
In chronic lymphocytic leukemia (CLL), monocytes and macrophages are skewed toward protumorigenic phenotypes, including the release of tumor-supportive cytokines and the expression of immunosuppressive molecules such as programmed cell death 1 ligand 1 (PD-L1). To understand the mechanism driving protumorigenic skewing in CLL, we evaluated the role of tumor cell–derived exosomes in the cross-talk with monocytes. We carried out RNA sequencing and proteome analyses of CLL-derived exosomes and identified noncoding Y RNA hY4 as a highly abundant RNA species that is enriched in exosomes from plasma of CLL patients compared with healthy donor samples. Transfer of CLL-derived exosomes or hY4 alone to monocytes resulted in key CLL-associated phenotypes, including the release of cytokines, such as C-C motif chemokine ligand 2 (CCL2), CCL4, and interleukin-6, and the expression of PD-L1. These responses were abolished in Toll-like receptor 7 (TLR7)–deficient monocytes, suggesting exosomal hY4 as a driver of TLR7 signaling. Pharmacologic inhibition of endosomal TLR signaling resulted in a substantially reduced activation of monocytes in vitro and attenuated CLL development in vivo. Our results indicate that exosome-mediated transfer of noncoding RNAs to monocytes contributes to cancer-related inflammation and concurrent immune escape via PD-L1 expression. [ABSTRACT FROM AUTHOR]
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- 2017
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16. The endosomal transcriptional regulator RNF11 integrates degradation and transport of EGFR.
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Scharaw, Sandra, Iskar, Murat, Ori, Alessandro, Boncompain, Gaelle, Laketa, Vibor, Poser, Ina, Lundberg, Emma, Perez, Franck, Beck, Martin, Bork, Peer, and Pepperkok, Rainer
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GENETIC transcription regulation , *ENDOSOMES , *EPIDERMAL growth factor receptors , *CELLULAR signal transduction , *CELL membranes - Abstract
Stimulation of cells with epidermal growth factor (EGF) induces internalization and partial degradation of the EGF receptor (EGFR) by the endo-lysosomal pathway. For continuous cell functioning, EGFR plasma membrane levels are maintained by transporting newly synthesized EGFRs to the cell surface. The regulation of this process is largely unknown. In this study, we find that EGF stimulation specifically increases the transport efficiency of newly synthesized EGFRs from the endoplasmic reticulum to the plasma membrane. This coincides with an up-regulation of the inner coat protein complex II (COP II) components SEC23B, SEC24B, and SEC24D, which we show to be specifically required for EGFR transport. Up-regulation of these COP II components requires the transcriptional regulator RNF11, which localizes to early endosomes and appears additionally in the cell nucleus upon continuous EGF stimulation. Collectively, our work identifies a new regulatory mechanism that integrates the degradation and transport of EGFR in order to maintain its physiological levels at the plasma membrane. [ABSTRACT FROM AUTHOR]
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- 2016
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17. Spatiotemporal variation of mammalian protein complex stoichiometries.
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Ori, Alessandro, Iskar, Murat, Buczak, Katarzyna, Kastritis, Panagiotis, Parca, Luca, Andrés-Pons, Amparo, Singer, Stephan, Bork, Peer, and Beck, Martin
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- 2016
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18. Characterization of drug-induced transcriptional modules: towards drug repositioning and functional understanding.
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Iskar, Murat, Zeller, Georg, Blattmann, Peter, Campillos, Monica, Kuhn, Michael, Kaminska, Katarzyna H., Runz, Heiko, Gavin, Anne-Claude, Pepperkok, Rainer, Noort, Vera van, and Bork, Peer
- Abstract
In pharmacology, it is crucial to understand the complex biological responses that drugs elicit in the human organism and how well they can be inferred from model organisms.We therefore identified a large set of drug-induced transcriptional modules from genome-wide microarray data of drugtreated human cell lines and rat liver, and first characterized their conservation. Over 70%of these modules were common for multiple cell lines and 15% were conserved between the human in vitro and the rat in vivo system. We then illustrate the utility of conserved and cell-type-specific druginduced modules by predicting and experimentally validating (i) gene functions, e.g., 10 novel regulators of cellular cholesterol homeostasis and (ii) new mechanisms of action for existing drugs, thereby providing a starting point for drug repositioning, e.g., novel cell cycle inhibitors and new modulators of a-adrenergic receptor, peroxisome proliferator-activated receptor and estrogen receptor. Taken together, the identified modules reveal the conservation of transcriptional responses towards drugs across cell types and organisms, and improve our understanding of both the molecular basis of drug action and human biology. [ABSTRACT FROM AUTHOR]
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- 2013
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19. Cell type-specific nuclear pores: a case in point for context-dependent stoichiometry of molecular machines.
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Ori, Alessandro, Banterle, Niccolò, Iskar, Murat, Andrés‐Pons, Amparo, Escher, Claudia, Khanh Bui, Huy, Sparks, Lenore, Solis‐Mezarino, Victor, Rinner, Oliver, Bork, Peer, Lemke, Edward A, and Beck, Martin
- Abstract
To understand the structure and function of large molecular machines, accurate knowledge of their stoichiometry is essential. In this study, we developed an integrated targeted proteomics and super-resolution microscopy approach to determine the absolute stoichiometry of the human nuclear pore complex (NPC), possibly the largest eukaryotic protein complex. We show that the human NPC has a previously unanticipated stoichiometry that varies across cancer cell types, tissues and in disease. Using large-scale proteomics, we provide evidence that more than one third of the known, well-defined nuclear protein complexes display a similar cell type-specific variation of their subunit stoichiometry. Our data point to compositional rearrangement as a widespread mechanism for adapting the functions of molecular machines toward cell type-specific constraints and context-dependent needs, and highlight the need of deeper investigation of such structural variants. [ABSTRACT FROM AUTHOR]
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- 2013
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20. Drug discovery in the age of systems biology: the rise of computational approaches for data integration
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Iskar, Murat, Zeller, Georg, Zhao, Xing-Ming, van Noort, Vera, and Bork, Peer
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DRUG design , *SYSTEMS biology , *DATA integration , *COMPUTER-aided design , *MOLECULAR biology , *PHARMACODYNAMICS , *COMPUTATIONAL biology , *DRUG monitoring - Abstract
The increased availability of large-scale open-access resources on bioactivities of small molecules has a significant impact on pharmacology facilitated mainly by computational approaches that digest the vast amounts of data. We discuss here how computational data integration enables systemic views on a drug''s action and allows to tackle complex problems such as the large-scale prediction of drug targets, drug repurposing, the molecular mechanisms, cellular responses or side effects. We particularly focus on computational methods that leverage various cell-based transcriptional, proteomic and phenotypic profiles of drug response in order to gain a systemic view of drug action at the molecular, cellular and whole-organism scale. [Copyright &y& Elsevier]
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- 2012
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21. Prediction of Drug Combinations by Integrating Molecular and Pharmacological Data.
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Xing-Ming Zhao, Iskar, Murat, Zeller, Georg, Kuhn, Michael, Van Noort, Vera, and Bork, Peer
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COMBINATION drug therapy , *DRUG efficacy , *DRUG side effects , *PHARMACEUTICAL chemistry , *DRUG metabolism , *PHARMACOKINETICS - Abstract
Combinatorial therapy is a promising strategy for combating complex disorders due to improved efficacy and reduced side effects. However, screening new drug combinations exhaustively is impractical considering all possible combinations between drugs. Here, we present a novel computational approach to predict drug combinations by integrating molecular and pharmacological data. Specifically, drugs are represented by a set of their properties, such as their targets or indications. By integrating several of these features, we show that feature patterns enriched in approved drug combinations are not only predictive for new drug combinations but also provide insights into mechanisms underlying combinatorial therapy. Further analysis confirmed that among our top ranked predictions of effective combinations, 69% are supported by literature, while the others represent novel potential drug combinations. We believe that our proposed approach can help to limit the search space of drug combinations and provide a new way to effectively utilize existing drugs for new purposes. [ABSTRACT FROM AUTHOR]
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- 2011
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22. Network Neighbors of Drug Targets Contribute to Drug Side-Effect Similarity.
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Brouwers, Lucas, Iskar, Murat, Zeller, Georg, Noort, Vera van, and Bork, Peer
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DRUG side effects , *DRUG target , *PHARMACOLOGY , *DRUG development , *BIOCHEMICAL mechanism of action , *PROTEINS , *PHARMACODYNAMICS - Abstract
In pharmacology, it is essential to identify the molecular mechanisms of drug action in order to understand adverse side effects. These adverse side effects have been used to infer whether two drugs share a target protein. However, side-effect similarity of drugs could also be caused by their target proteins being close in a molecular network, which as such could cause similar downstream effects. In this study, we investigated the proportion of side-effect similarities that is due to targets that are close in the network compared to shared drug targets. We found that only a minor fraction of side-effect similarities (5.8 %) are caused by drugs targeting proteins close in the network, compared to side-effect similarities caused by overlapping drug targets (64%). Moreover, these targets that cause similar side effects are more often in a linear part of the network, having two or less interactions, than drug targets in general. Based on the examples, we gained novel insight into the molecular mechanisms of side effects associated with several drug targets. Looking forward, such analyses will be extremely useful in the process of drug development to better understand adverse side effects. [ABSTRACT FROM AUTHOR]
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- 2011
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23. Drug-Induced Regulation of Target Expression.
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Iskar, Murat, Campillos, Monica, Kuhn, Michael, Jensen, Lars Juhl, van Noort, Vera, and Bork, Peer
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TARGETED drug delivery , *GENE targeting , *GENE expression , *MESSENGER RNA , *GENETIC regulation , *DNA topoisomerase II - Abstract
Drug perturbations of human cells lead to complex responses upon target binding. One of the known mechanisms is a (positive or negative) feedback loop that adjusts the expression level of the respective target protein. To quantify this mechanism systems-wide in an unbiased way, drug-induced differential expression of drug target mRNA was examined in three cell lines using the Connectivity Map. To overcome various biases in this valuable resource, we have developed a computational normalization and scoring procedure that is applicable to gene expression recording upon heterogeneous drug treatments. In 1290 drug-target relations, corresponding to 466 drugs acting on 167 drug targets studied, 8% of the targets are subject to regulation at the mRNA level. We confirmed systematically that in particular G-protein coupled receptors, when serving as known targets, are regulated upon drug treatment. We further newly identified drug-induced differential regulation of Lanosterol 14-alpha demethylase, Endoplasmin, DNA topoisomerase 2-alpha and Calmodulin 1. The feedback regulation in these and other targets is likely to be relevant for the success or failure of the molecular intervention. [ABSTRACT FROM AUTHOR]
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- 2010
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24. CART—a chemical annotation retrieval toolkit.
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Deghou, Samy, Zeller, Georg, Iskar, Murat, Driessen, Marja, Castillo, Mercedes, van Noort, Vera, and Bork, Peer
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DATABASES ,INFORMATION storage & retrieval systems ,DIGITAL resources in the pharmaceutical industry ,PHARMACOLOGY ,WEB services ,COMPUTER network resources - Abstract
Motivation: Data on bioactivities of drug-like chemicals are rapidly accumulating in public repositories, creating new opportunities for research in computational systems pharmacology. However, integrative analysis of these data sets is difficult due to prevailing ambiguity between chemical names and identifiers and a lack of cross-references between databases. Results: To address this challenge, we have developed CART, a Chemical Annotation Retrieval Toolkit. As a key functionality, it matches an input list of chemical names into a comprehensive reference space to assign unambiguous chemical identifiers. In this unified space, bioactivity annotations can be easily retrieved from databases covering a wide variety of chemical effects on biological systems. Subsequently, CART can determine annotations enriched in the input set of chemicals and display these in tabular format and interactive network visualizations, thereby facilitating integrative analysis of chemical bioactivity data. [ABSTRACT FROM AUTHOR]
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- 2016
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25. ATRT-09. INTEGRATIVE ANALYSES OF GENE REGULATORY LANDSCAPES REVEAL RHABDOID TUMOR SUBGROUPS WITH POSSIBLE IMMUNE MODULATION THROUGH EPIGENETIC DYSREGULATION.
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Johann, Pascal, Chun, Hye-Jung, Erkek, Serap, Iskar, Murat, Perlman, Elizabeth, Hasselblatt, Martin, Pfister, Stefan M, Marra, Marco, and Kool, Marcel
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- 2019
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26. Identification and Analyses of Extra-Cranial and Cranial Rhabdoid Tumor Molecular Subgroups Reveal Tumors with Cytotoxic T Cell Infiltration.
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Chun, Hye-Jung E., Johann, Pascal D., Milne, Katy, Zapatka, Marc, Buellesbach, Annette, Ishaque, Naveed, Iskar, Murat, Erkek, Serap, Wei, Lisa, Tessier-Cloutier, Basile, Lever, Jake, Titmuss, Emma, Topham, James T., Bowlby, Reanne, Chuah, Eric, Mungall, Karen L., Ma, Yussanne, Mungall, Andrew J., Moore, Richard A., and Taylor, Michael D.
- Abstract
Extra-cranial malignant rhabdoid tumors (MRTs) and cranial atypical teratoid RTs (ATRTs) are heterogeneous pediatric cancers driven primarily by SMARCB1 loss. To understand the genome-wide molecular relationships between MRTs and ATRTs, we analyze multi-omics data from 140 MRTs and 161 ATRTs. We detect similarities between the MYC subgroup of ATRTs (ATRT-MYC) and extra-cranial MRTs, including global DNA hypomethylation and overexpression of HOX genes and genes involved in mesenchymal development, distinguishing them from other ATRT subgroups that express neural-like features. We identify five DNA methylation subgroups associated with anatomical sites and SMARCB1 mutation patterns. Groups 1, 3, and 4 exhibit cytotoxic T cell infiltration and expression of immune checkpoint regulators, consistent with a potential role for immunotherapy in rhabdoid tumor patients. • MYC subgroup of cranial RTs (ATRT-MYC) is molecularly similar to extra-cranial RTs • Five DNA methylation subgroups are identified in RTs across multiple organ sites • Groups 1, 3, and 4 exhibit cytotoxic T cell infiltration and PD1 and PD-L1 expression Chun et al. report similarities between the MYC subgroup of cranial and extra-cranial rhabdoid tumors (RTs) at genetic, gene-expression, and epigenetic levels. They identify five DNA methylation subgroups of RTs across multiple organ sites, and some subgroups exhibit increased levels of immune cell infiltration and immune checkpoint expression. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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27. Linking aberrant chromatin features in chronic lymphocytic leukemia to transcription factor networks.
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Mallm, Jan‐Philipp, Iskar, Murat, Ishaque, Naveed, Klett, Lara C, Kugler, Sabrina J, Muino, Jose M, Teif, Vladimir B, Poos, Alexandra M, Großmann, Sebastian, Erdel, Fabian, Tavernari, Daniele, Koser, Sandra D, Schumacher, Sabrina, Brors, Benedikt, König, Rainer, Remondini, Daniel, Vingron, Martin, Stilgenbauer, Stephan, Lichter, Peter, and Zapatka, Marc
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CHRONIC lymphocytic leukemia , *CHROMATIN , *TRANSCRIPTION factors , *DNA methylation , *GENE regulatory networks - Abstract
In chronic lymphocytic leukemia (CLL), a diverse set of genetic mutations is embedded in a deregulated epigenetic landscape that drives cancerogenesis. To elucidate the role of aberrant chromatin features, we mapped DNA methylation, seven histone modifications, nucleosome positions, chromatin accessibility, binding of EBF1 and CTCF, as well as the transcriptome of B cells from CLL patients and healthy donors. A globally increased histone deacetylase activity was detected and half of the genome comprised transcriptionally downregulated partially DNA methylated domains demarcated by CTCF. CLL samples displayed a H3K4me3 redistribution and nucleosome gain at promoters as well as changes of enhancer activity and enhancer linkage to target genes. A DNA binding motif analysis identified transcription factors that gained or lost binding in CLL at sites with aberrant chromatin features. These findings were integrated into a gene regulatory enhancer containing network enriched for B‐cell receptor signaling pathway components. Our study predicts novel molecular links to targets of CLL therapies and provides a valuable resource for further studies on the epigenetic contribution to the disease. Synopsis: Transcriptome profiling and genome‐scale mapping of DNA methylation, seven histone modifications, nucleosome positions, chromatin accessibility, EBF1 and CTCF binding are performed in B cells from CLL patients and healthy donors. Altered chromatin features were detected at 80% of the differentially regulated genes in CLL and histone deacetylase activity was globally increased.Half of the CLL genome comprised partially DNA methylated domains that were transcriptionally downregulated, demarcated by CTCF and enriched for H3K9me3 and H3K27me3.H3K4me3 was redistributed at CLL promoters, including loss of bivalent H3K4me3/H3K27me3 states, and substantial changes of enhancer activity were detected.A gene regulatory network including enhancers was constructed around the transcription factors targeting 15 central binding motifs that were associated with aberrant CLL chromatin features. Genes involved in BCR signaling were enriched in the network. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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28. Novel Drug Candidates for the Treatment of Metastatic Colorectal Cancer through Global Inverse Gene-Expression Profiling.
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van Noort, Vera, Schölch, Sebastian, Iskar, Murat, Zeller, Georg, Ostertag, Kristina, Schweitzer, Christine, Werner, Kristin, Weitz, Jürgen, Koch, Moritz, and Bork, Peer
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COLON cancer treatment , *GENE expression , *CITALOPRAM , *TROGLITAZONE , *TRANSFORMING growth factors-beta , *THERAPEUTICS - Abstract
Drug-induced gene-expression profiles that invert disease profiles have recently been illustrated to be a starting point for drug repositioning. In this study, we validate this approach and focus on prediction of novel drugs for colorectal cancer, for which there is a pressing need to find novel antimetastatic compounds. We computationally predicted three novel and still unknown compounds against colorectal cancer: citalopram (an antidepressant), troglitazone (an antidiabetic), and enilconazole (a fungicide). We verified the compounds by in vitro assays of clonogenic survival, proliferation, and migration and in a subcutaneous mouse model. We found evidence that the mode of action of these compounds may be through inhibition of TGFβ signaling. Furthermore, one compound, citalopram, reduced tumor size as well as the number of circulating tumor cells and metastases in an orthotopic mouse model of colorectal cancer. This study proposes citalopram as a potential therapeutic option for patients with colorectal cancer, illustrating the potential of systems pharmacology [ABSTRACT FROM AUTHOR]
- Published
- 2014
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29. Interleukin-10 receptor signaling promotes the maintenance of a PD-1int TCF-1+ CD8+ T cell population that sustains anti-tumor immunity.
- Author
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Hanna, Bola S., Llaó-Cid, Laura, Iskar, Murat, Roessner, Philipp M., Klett, Lara C., Wong, John K.L., Paul, Yashna, Ioannou, Nikolaos, Öztürk, Selcen, Mack, Norman, Kalter, Verena, Colomer, Dolors, Campo, Elías, Bloehdorn, Johannes, Stilgenbauer, Stephan, Dietrich, Sascha, Schmidt, Manfred, Gabriel, Richard, Rippe, Karsten, and Feuerer, Markus
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T cells , *CELL populations , *CD8 antigen , *AP-1 transcription factor , *INTERLEUKIN-10 , *CHRONIC leukemia - Abstract
T cell exhaustion limits anti-tumor immunity and responses to immunotherapy. Here, we explored the microenvironmental signals regulating T cell exhaustion using a model of chronic lymphocytic leukemia (CLL). Single-cell analyses identified a subset of PD-1hi, functionally impaired CD8+ T cells that accumulated in secondary lymphoid organs during disease progression and a functionally competent PD-1int subset. Frequencies of PD-1int TCF-1+ CD8+ T cells decreased upon Il10rb or Stat3 deletion, leading to accumulation of PD-1hi cells and accelerated tumor progression. Mechanistically, inhibition of IL-10R signaling altered chromatin accessibility and disrupted cooperativity between the transcription factors NFAT and AP-1, promoting a distinct NFAT-associated program. Low IL10 expression or loss of IL-10R-STAT3 signaling correlated with increased frequencies of exhausted CD8+ T cells and poor survival in CLL and in breast cancer patients. Thus, balance between PD-1hi, exhausted CD8+ T cells and functional PD-1int TCF-1+ CD8+ T cells is regulated by cell-intrinsic IL-10R signaling, with implications for immunotherapy. [Display omitted] • Heterogeneous PD-1 expression identifies CD8+ T cells with distinct functions in CLL • IL-10 prevents activation-induced exhaustion of CD8+ T cells and reduces CLL development • By altering chromatin in CD8+ T cells, IL-10R blockade disrupts NFAT:AP-1 cooperativity • IL-10R loss correlates with T cell exhaustion and poor survival of cancer patients T cell exhaustion limits anti-tumor immunity and responses to immunotherapy. Using a model of chronic lymphocytic leukemia, Hanna, Llaó-Cid, et al. reveal that the balance between PD-1hi, exhausted CD8+ T cells and functional PD-1int CD8+ T cells that are associated with tumor progression or tumor control, respectively, is regulated by cell-intrinsic IL-10R signaling. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
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30. IL4I1 Is a Metabolic Immune Checkpoint that Activates the AHR and Promotes Tumor Progression.
- Author
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Sadik, Ahmed, Somarribas Patterson, Luis F., Öztürk, Selcen, Mohapatra, Soumya R., Panitz, Verena, Secker, Philipp F., Pfänder, Pauline, Loth, Stefanie, Salem, Heba, Prentzell, Mirja Tamara, Berdel, Bianca, Iskar, Murat, Faessler, Erik, Reuter, Friederike, Kirst, Isabelle, Kalter, Verena, Foerster, Kathrin I., Jäger, Evelyn, Guevara, Carina Ramallo, and Sobeh, Mansour
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CANCER cell motility , *CANCER invasiveness , *ARYL hydrocarbon receptors , *CHRONIC lymphocytic leukemia , *NATURAL language processing , *CELL motility , *PROGRAMMED cell death 1 receptors - Abstract
Aryl hydrocarbon receptor (AHR) activation by tryptophan (Trp) catabolites enhances tumor malignancy and suppresses anti-tumor immunity. The context specificity of AHR target genes has so far impeded systematic investigation of AHR activity and its upstream enzymes across human cancers. A pan-tissue AHR signature, derived by natural language processing, revealed that across 32 tumor entities, interleukin-4-induced-1 (IL4I1) associates more frequently with AHR activity than IDO1 or TDO2 , hitherto recognized as the main Trp-catabolic enzymes. IL4I1 activates the AHR through the generation of indole metabolites and kynurenic acid. It associates with reduced survival in glioma patients, promotes cancer cell motility, and suppresses adaptive immunity, thereby enhancing the progression of chronic lymphocytic leukemia (CLL) in mice. Immune checkpoint blockade (ICB) induces IDO1 and IL4I1. As IDO1 inhibitors do not block IL4I1, IL4I1 may explain the failure of clinical studies combining ICB with IDO1 inhibition. Taken together, IL4I1 blockade opens new avenues for cancer therapy. • A pan-tissue AHR signature identifies IL4I1 as a major AHR-activating enzyme • IL4I1-mediated Trp catabolism yields indoles and kynurenic acid that activate the AHR • IL4I1 promotes AHR-driven cancer cell motility and suppresses adaptive immunity • IL4I1 enhances CLL progression and is induced by immune checkpoint blockade Immune checkpoint blockade therapy induces the AHR-activating enzyme IL4I1, which promotes tumor progression, through its effects on tumor cell motility and adaptive immunity. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
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