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Cell type-specific nuclear pores: a case in point for context-dependent stoichiometry of molecular machines.
- Source :
- Molecular Systems Biology; 2013, Vol. 9 Issue 1, p1-N.PAG, 11p, 6 Graphs
- Publication Year :
- 2013
-
Abstract
- To understand the structure and function of large molecular machines, accurate knowledge of their stoichiometry is essential. In this study, we developed an integrated targeted proteomics and super-resolution microscopy approach to determine the absolute stoichiometry of the human nuclear pore complex (NPC), possibly the largest eukaryotic protein complex. We show that the human NPC has a previously unanticipated stoichiometry that varies across cancer cell types, tissues and in disease. Using large-scale proteomics, we provide evidence that more than one third of the known, well-defined nuclear protein complexes display a similar cell type-specific variation of their subunit stoichiometry. Our data point to compositional rearrangement as a widespread mechanism for adapting the functions of molecular machines toward cell type-specific constraints and context-dependent needs, and highlight the need of deeper investigation of such structural variants. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17444292
- Volume :
- 9
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Molecular Systems Biology
- Publication Type :
- Academic Journal
- Accession number :
- 110555153
- Full Text :
- https://doi.org/10.1038/msb.2013.4