109 results on '"Dongdong Su"'
Search Results
2. A probe for NIR-II imaging and multimodal analysis of early Alzheimer’s disease by targeting CTGF
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Cao Lu, Cong Meng, Yuying Li, Jinling Yuan, Xiaojun Ren, Liang Gao, Dongdong Su, Kai Cao, Mengchao Cui, Qing Yuan, and Xueyun Gao
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Science - Abstract
Abstract To date, earlier diagnosis of Alzheimer’s disease (AD) is still challenging. Recent studies revealed the elevated expression of connective tissue growth factor (CTGF) in AD brain is an upstream regulator of amyloid-beta (Aβ) plaque, thus CTGF could be an earlier diagnostic biomarker of AD than Aβ plaque. Herein, we develop a peptide-coated gold nanocluster that specifically targets CTGF with high affinity (KD ~ 21.9 nM). The probe can well penetrate the blood-brain-barrier (BBB) of APP/PS1 transgenic mice at early-stage (earlier than 3-month-old) in vivo, allowing non-invasive NIR-II imaging of CTGF when there is no appearance of Aβ plaque deposition. Notably, this probe can also be applied to measuring CTGF on postmortem brain sections by multimodal analysis, including fluorescence imaging, peroxidase-like chromogenic imaging, and ICP-MS quantitation, which enables distinguishment between the brains of AD patients and healthy people. This probe possesses great potential for precise diagnosis of earlier AD before Aβ plaque formation.
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- 2024
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3. Visual genetic typing of glioma using proximity‐anchored in situ spectral coding amplification
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Xiaolei Chen, Ruijie Deng, Dongdong Su, Xiaochen Ma, Xu Han, Shizheng Wang, Yuqing Xia, Zifu Yang, Ningqiang Gong, Yanwei Jia, Xueyun Gao, and Xiaojun Ren
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genetic typing ,glioma ,in situ imaging ,multiplexed detection ,RNA mutation ,Biotechnology ,TP248.13-248.65 - Abstract
Abstract Gliomas are histologically and genetically heterogeneous tumors. However, classical histopathological typing often ignores the high heterogeneity of tumors and thus cannot meet the requirements of precise pathological diagnosis. Here, proximity‐anchored in situ spectral coding amplification (ProxISCA) is proposed for multiplexed imaging of RNA mutations, enabling visual typing of brain gliomas with different pathological grades at the single‐cell and tissue levels. The ligation‐based padlock probe can discriminate one‐nucleotide variations, and the design of proximity primers enables the anchoring of amplicons on target RNA, thus improving localization accuracy. The DNA module‐based spectral coding strategy can dramatically improve the multiplexing capacity for imaging RNA mutations through one‐time labelling, with low cost and simple operation. One‐target‐one‐amplicon amplification confers ProxISCA the ability to quantify RNA mutation copy number with single‐molecule resolution. Based on this approach, it is found that gliomas with higher malignant grades express more genes with high correlation at the cellular and tissue levels and show greater cellular heterogeneity. ProxISCA provides a tool for glioma research and precise diagnosis, which can reveal the relationship between cellular heterogeneity and glioma occurrence or development and assist in pathological prognosis.
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- 2023
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4. Extended Pantograph–Catenary Arc Modeling and an Analysis of the Vehicular-Grounding Electromagnetic Transients of Electric Multiple Units
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Ke Huang and Dongdong Su
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arc extinction and arc reignition ,arc length ,arc modeling ,high-speed railway ,pantograph–catenary arcing ,train body over-voltages ,Technology - Abstract
As the operating speed of electric multiple units (EMUs) in high-speed railways increases, pantograph–catenary (PC) detachment arcing occurs frequently. The resulting vehicular-grounding electromagnetic transients are related to the dynamic characteristics of the arc length. During large detachment, the processes of arc extinction and arc reignition may occur, resulting in more severe train body (TB) over-voltages and adverse effects on some vehicular electronic devices. As an extension of the previous works, this paper aims to establish a suitable PC arc model to examine the TB transient voltages. To begin with, the arc length dynamic characteristics are reasonably analyzed to deduce the relationship between the detachment distance and the arc length via the chain arc model. Then, the dynamic characteristics of the arc length are introduced, and an arc modeling scheme is proposed to elaborate the vehicle-grid electric power model for EMUs encountering various arcing scenarios. Based on this, the transient over-voltages are analyzed, accounting for both the arc extinction and arc reignition, as well as the mutual influences of multiple detachments in a short time. The influential factors, including arc length characteristics, phase angle, excitation inductance, and grounding parameters, are also involved in the performed analyses.
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- 2024
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5. Enhancing Liver Delivery of Gold Nanoclusters via Human Serum Albumin Encapsulation for Autoimmune Hepatitis Alleviation
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Cong Meng, Yu Liu, Yuping Ming, Cao Lu, Yanggege Li, Yulu Zhang, Dongdong Su, Xueyun Gao, and Qing Yuan
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peptide-conjugated gold nanoclusters ,autoimmune hepatitis ,liver delivery ,human serum albumin encapsulation ,IFN-γ/STAT ,Pharmacy and materia medica ,RS1-441 - Abstract
Peptide-protected gold nanoclusters (AuNCs), possessing exceptional biocompatibility and remarkable physicochemical properties, have demonstrated intrinsic pharmaceutical activity in immunomodulation, making them a highly attractive frontier in the field of nanomedicine exploration. Autoimmune hepatitis (AIH) is a serious autoimmune liver disease caused by the disruption of immune balance, for which effective treatment options are still lacking. In this study, we initially identified glutathione (GSH)-protected AuNCs as a promising nanodrug candidate for AIH alleviating in a Concanavalin A (Con A)-induced mice model. However, to enhance treatment efficiency, liver-targeted delivery needs to be improved. Therefore, human serum albumin (HSA)-encapsulated AuNCs were constructed to achieve enhanced liver targeting and more potent mitigation of Con A-induced elevations in plasma aspartate transaminase (AST), alanine transaminase (ALT), and liver injury in mice. In vivo and in vitro mechanism studies indicated that AuNCs could suppress the secretion of IFN-γ by Con A-stimulated T cells and subsequently inhibit the activation of the JAK2/STAT1 pathway and eventual hepatocyte apoptosis induced by IFN-γ. These actions ultimately protect the liver from immune cell infiltration and damage caused by Con A. These findings suggest that bio-protected AuNCs hold promise as nanodrugs for AIH therapy, with their liver targeting capabilities and therapeutic efficiency being further improved via rational surface ligand engineering.
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- 2024
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6. Image‐guided diagnosis and treatment of glioblastoma
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Yongning Bian, Yaling Wang, Xueqian Chen, Yong Zhang, Shaoqing Xiong, and Dongdong Su
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diagnosis ,glioblastoma ,image‐guided ,optical imaging ,therapy ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Abstract Glioblastoma (GBM) is the most aggressive primary brain tumor with poor prognosis and high recurrence rate. The presence of the blood–brain barrier (BBB) prevents diagnostic and therapeutic drugs from penetrating and working in GBM. In traditional surgical treatment, it is difficult to completely distinguish the boundary between tumor and surrounding normal tissue, resulting in incomplete resection of tumor. Therefore, the diagnosis and treatment of GBM are very challenging. Several molecular probes and nanoprobes have been reported to successfully penetrate the BBB, selectively target and accumulate in GBM to achieve in situ imaging of brain tumors, thus achieving accurate diagnosis and treatment of orthotopic or non‐orthotopic GBM. This paper reviews the advances of molecular probes and nanoprobes in image‐guided diagnosis and treatment of GBM. The design principle, application, and advantages of each probe are enumerated in detail. Finally, the prospects and potential challenges of probes in the diagnosis and treatment of GBM are discussed with a view to further promote the study and application of novel imaging probes in GBM.
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- 2023
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7. Recent Progress in Fluorescent Probes for the Detection and Research of Hydrogen Sulfide in Cells
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Weier Liang, Yong Zhang, Shaoqing Xiong, and Dongdong Su
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hydrogen sulfide (H2S) ,fluorescent probe ,cell imaging ,Biochemistry ,QD415-436 - Abstract
Hydrogen sulfide (H2S) is a gaseous signaling molecule that plays an important role in regulating various physiological activities in biological systems. As the fundamental structural and functional unit of organisms, cells are closely related to the homeostasis of their internal environment. The levels of H2S in different organelles maintain a certain balance, and any disruption of this balance will lead to various functional abnormalities that affect the health of organisms. Fluorescent imaging technology provides unique merits, such as simplicity, non-invasiveness, and real-time monitoring, and has become a powerful approach for the detection of molecules in biological systems. Based on the special physicochemical properties of H2S, numerous H2S-specific fluorogenic probes have been designed with different recognition mechanisms that enable rapid and accurate detection of H2S in cells. Therefore, this review briefly illustrates the design strategies, response principles, and biological applications of H2S-specific fluorescent probes and aims to provide relevant researchers with insight for future research.
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- 2023
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8. Optimal Cooperative Wireless Communication for Mobile User Data Offloading
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Guangsheng Feng, Fumin Xia, Yongmin Zhang, Dongdong Su, Haibin Lv, Huiqiang Wang, and Hongwu Lv
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Mobile data offloading ,bi-level optimization ,utility function ,user satisfaction ,cooperative transmission ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 - Abstract
We study the mobile data offloading problem in cooperative cellular networks, in which the cellular base station can offload a fraction of data traffic to its cooperative Wi-Fi networks. Both user satisfaction and benefit are important for the cellular network. Under the case that the cellular network cannot provide sufficient transmission resources, part of data traffic can be offloaded to its cooperative Wi-Fi networks to guarantee the user satisfaction, even though the benefit loss of the cellular network will be incurred. Meanwhile, considering the users are price sensitive, the operators, including cellular and Wi-Fi, can issue an appreciate unit price to attract more users and more traffic requirements to increase their total benefit. In this paper, we intend to design an optimal pricing scheme to maximize the user satisfaction and minimize the benefit losses of the operators simultaneously. First, we formulate the mobile data offloading problem as a joint optimization of “min–max” problem. Then, we convert the problem into a bi-level optimization problem, in which the lower level is to maximize the user satisfaction and the upper level is to minimize the benefit losses of the operators. Since the lower-level problem is convex, a centralized scheme based on the dynamic coordinate search-based method is employed to find the optimal solution of the bi-level problem. In addition, a distributed method is proposed to obtain the near-optimal solution quickly, where one-third-party agent is used to adjust the data traffic allocation. Simulation results show that the proposed method achieves the near-optimal solutions with limited iterations.
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- 2018
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9. Development of background-free tame fluorescent probes for intracellular live cell imaging
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Samira Husen Alamudi, Rudrakanta Satapathy, Jihyo Kim, Dongdong Su, Haiyan Ren, Rajkumar Das, Lingna Hu, Enrique Alvarado-Martínez, Jung Yeol Lee, Christian Hoppmann, Eduardo Peña-Cabrera, Hyung-Ho Ha, Hee-Sung Park, Lei Wang, and Young-Tae Chang
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Science - Abstract
The success of a fluorescent dye as a molecular probe to monitor the intracellular activity of biomolecules depends on its physicochemical characteristics. Here, the authors use a predictive model to identify key features that allow them to design cell permeable, background-free fluorescent probes.
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- 2016
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10. Optimal transmission for scalable video coded streaming in cellular wireless networks with the cooperation of local peer-to-peer network
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Guangsheng Feng, Junyu Lin, Qian Zhao, Huiqiang Wang, Hongwu Lv, Bingyang Li, and DongDong Su
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Electronic computers. Computer science ,QA75.5-76.95 - Abstract
We study the problem of transmitting scalable video coded streams in a hybrid scenario including a global cellular network and a local peer-to-peer network. Given desirable channel condition in cellular downlink, some cellular user equipments can fetch the video chunks via the cellular base station directly, and also provide relay service via the local peer-to-peer network for other user equipments unable to meet their basic video quality. To take the advantage of channel diversity gains, the method of random linear coding is adopted to generate linear combinations of the video chunks for transmission in both the cellular and peer-to-peer networks. To optimize the cellular and peer-to-peer transmission arrangement, we first formulate it as a mixed integer nonlinear programming problem, which becomes more complicated when the number of user equipments increases. Then, we convert it to a quasi-convex optimization problem by approximating the indicator function with a continuous step function, and it can be solved by a centralized approach. Furthermore, a primal-dual decomposition approach is developed and a distributed algorithm is proposed accordingly. Simulation results show that the proposed approach achieves a near-optimal solution.
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- 2017
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11. Spatial Magnetic-Field Description Method Aimed at 2 × 25 kV Auto-Transformer Power Supply System in High-Speed Railway
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Yunchuan Deng, Ke Huang, Dongdong Su, and Zhigang Liu
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high-speed railway (HSR) ,spatial magnetic-field description method ,auto-transformer (AT) power supply ,real loop circuits ,traction network integrated impedance ,current distribution ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Complete and accurate spatial magnetic field description is the premise of effectively assessing the power supply capability of a high-speed railway (HSR). Its evaluation indicators are the current distributions and the integrated impedance of traction network. This paper proposes a spatial magnetic-field description method for the auto-transformer (AT) power supply system. Due to the limitations of previous approaches, all the real loop circuits of the AT system are considered for structuring a loop circuit matrix. At first, different description processes are divided, respectively, into those for the right side and the left side of the load. Then, considering that two types of return conductors exist in an AT system, a certain current ratio deduced in existing studies is introduced. As the introduced current ratio of the left side of the load is approximate, an iterative scheme is adopted. By constantly adjusting current ratio to satisfy a constraint condition of circuit voltages, accurate traction network impedance is obtained. Last, in order to verify the effectiveness of the proposed description method, two real-life experiments in a Chinese HSR line are performed, which indicate that the proposed method can not only directly reflect complete and accurate current distribution, but also deduce the exact traction network integrated impedance.
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- 2018
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12. Prevalence patterns of avian Plasmodium and Haemoproteus parasites and the influence of host relative abundance in southern China.
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Yanhua Zhang, Yuchun Wu, Qiang Zhang, Dongdong Su, and Fasheng Zou
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Medicine ,Science - Abstract
Infectious diseases threaten the health and survival of wildlife populations. Consequently, relationships between host diversity, host abundance, and parasite infection are important aspects of disease ecology and conservation research. Here, we report on the prevalence patterns of avian Plasmodium and Haemoproteus infections and host relative abundance influence based on sampling 728 wild-caught birds representing 124 species at seven geographically widespread sites in southern China. The overall prevalence of two haemoprotozoan parasites, Plasmodium and Haemoproteus, was 29.5%, with 22.0% attributable to Haemoproteus and 7.8% to Plasmodium. Haemoproteus prevalence differed significantly among different avian host families, with the highest prevalence in Nectariniidae, Pycnonotidae and Muscicapidae, whereas Plasmodium prevalence varied significantly among host species. Seventy-nine mitochondrial lineages including 25 from Plasmodium and 54 from Haemoproteus were identified, 80% of which were described here for the first time. The phylogenetic relationships among these parasites indicated stronger host-species specificity for Haemoproteus than Plasmodium. Well-supported host-family (Timaliidae) specific clades were found in both Plasmodium and Haemoproteus. The Haemoproteus tree shows regional subclades, whereas the Plasmodium clades are "scattered" among different geographical regions. Interestingly, there were statistically significant variations in the prevalence of Plasmodium and Haemoproteus among the geographical regions. Furthermore, the prevalence of Plasmodium and Haemoproteus were not significantly correlated with host relative abundance. Further efforts will focus on exploring the relationships between parasite prevalence and sex, age, and immune defense of the host.
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- 2014
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13. A Simple BODIPY-Based Viscosity Probe for Imaging of Cellular Viscosity in Live Cells
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Dongdong Su, Chai Lean Teoh, Nengyue Gao, Qing-Hua Xu, and Young-Tae Chang
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BODIPY ,fluorescent probes ,sensors ,viscosity ,FLIM ,cell imaging ,Chemical technology ,TP1-1185 - Abstract
Intracellular viscosity is a fundamental physical parameter that indicates the functioning of cells. In this work, we developed a simple boron-dipyrromethene (BODIPY)-based probe, BTV, for cellular mitochondria viscosity imaging by coupling a simple BODIPY rotor with a mitochondria-targeting unit. The BTV exhibited a significant fluorescence intensity enhancement of more than 100-fold as the solvent viscosity increased. Also, the probe showed a direct linear relationship between the fluorescence lifetime and the media viscosity, which makes it possible to trace the change of the medium viscosity. Furthermore, it was demonstrated that BTV could achieve practical applicability in the monitoring of mitochondrial viscosity changes in live cells through fluorescence lifetime imaging microscopy (FLIM).
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- 2016
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14. CRISPR-Cas9 gene editing strengthens cuproptosis/chemodynamic/ferroptosis synergistic cancer therapy
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Xiaoyu Wu, Zijun Bai, Hui Wang, Hanqing Wang, Dahai Hou, Yunzhu Xu, Guanqun Wo, Haibo Cheng, Dongdong Sun, and Weiwei Tao
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GSH- and pH-responsive ,Effective copper delivery ,CRISPR-Cas9 ,ATP7A ,Cuproptosis ,Chemodynamic ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Copper-based nanomaterials demonstrate promising potential in cancer therapy. Cu+ efficiently triggers a Fenton-like reaction and further consumes the high level of glutathione, initiating chemical dynamic therapy (CDT) and ferroptosis. Cuproptosis, a newly identified cell death modality that represents a great prospect in cancer therapy, is activated. However, active homeostatic systems rigorously keep copper levels within cells exceptionally low, which hinders the application of cooper nanomaterials-based therapy. Herein, a novel strategy of CRISPR-Cas9 RNP nanocarrier to deliver cuprous ions and suppress the expression of copper transporter protein ATP7A for maintaining a high level of copper in cytoplasmic fluid is developed. The Cu2O and organosilica shell would degrade under the high level of glutathione and weak acidic environment, further releasing RNP and Cu+. The liberated Cu+ triggered a Fenton-like reaction for CDT and partially transformed to Cu2+, consuming intracellular GSH and initiating cuproptosis and ferroptosis efficiently. Meanwhile, the release of RNP effectively reduced the expression of copper transporter ATP7A, subsequently increasing the accumulation of cooper and enhancing the efficacy of CDT, cuproptosis, and ferroptosis. Such tumor microenvironment responsive multimodal nanoplatform opens an ingenious avenue for colorectal cancer therapy based on gene editing enhanced synergistic cuproptosis/CDT/ferroptosis.
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- 2024
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15. Enzyme-activated near-infrared fluorogenic probe with high-efficiency intrahepatic targeting ability for visualization of drug-induced liver injury†
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Yaling Wang, Mingrui Li, Yong Zhang, Xueqian Chen, Xueyun Gao, Dongdong Su, Qing Yuan, and Yongning Bian
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Liver injury ,chemistry.chemical_classification ,Drug ,Fluorophore ,media_common.quotation_subject ,digestive, oral, and skin physiology ,Cholic acid ,General Chemistry ,medicine.disease ,chemistry.chemical_compound ,Chemistry ,Liver targeting ,Enzyme ,chemistry ,In vivo ,medicine ,Biophysics ,Leucine ,human activities ,media_common - Abstract
Hepatotoxicity is a serious problem faced by thousands of clinical drugs, and drug-induced liver injury (DILI) caused by chronic administration or overdose has become a major biosafety issue. However, the near-infrared (NIR) fluorescent probes currently used for liver injury detection still suffer from poor liver targeting ability and low sensitivity. Enzyme-activated fluorogenic probes with powerful in situ targeting ability are the key to improving the imaging effect of liver injury. Herein, we rationally designed a leucine aminopeptidase (LAP) activated fluorogenic probe hCy-CA-LAP, which greatly improved the hepatocyte-targeting capability by introducing a cholic acid group. The probe hCy-CA-LAP is converted into a high-emission hCy-CA fluorophore in the presence of LAP, showing high selectivity, high sensitivity and low detection limit (0.0067 U mL−1) for LAP, and successfully realizes the sensitive detection of small fluctuations of LAP in living cells. Moreover, the probe can achieve effective in situ accumulation in the liver, thereby achieving precise imaging and evaluation of two different types of drug-induced hepatotoxicity in vivo. Therefore, the probe hCy-CA-LAP may be a potential tool for exploring the roles of LAP and evaluating the degree of DILI., We rationally designed a leucine aminopeptidase (LAP) activated fluorogenic probe hCy-CA-LAP with high hepatocyte-targeting ability for accurate and sensitive imaging of DILI.
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- 2021
16. Intelligent Generation and Analysis of the Municipal Road Construction Scheme Based on the KNN Algorithm
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Chenzhong, Ren, primary, Wenliang, Kan, additional, Taihua, Zhou, additional, Dongdong, Su, additional, YongLi, Geng, additional, and Wenzheng, Wu, additional
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- 2022
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17. Cell membranes targeted unimolecular prodrug for programmatic photodynamic-chemo therapy
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Dongdong Su, Jie Yuan, Xiao-Bing Zhang, Xiujuan Zhuang, Hepeng Zhao, Xing-Xing Zhang, Mei Chen, Lin Yuan, and Rong Peng
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Combination therapy ,medicine.medical_treatment ,Cell ,Medicine (miscellaneous) ,Photodynamic therapy ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,two-photon photodynamic therapy ,combination therapy ,In vivo ,medicine ,Animals ,Humans ,Prodrugs ,Photosensitizer ,glutathione ,Hypoxia ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Fluorescent Dyes ,Photons ,Chemotherapy ,Photosensitizing Agents ,Singlet Oxygen ,Staining and Labeling ,Tumor hypoxia ,Rhodamines ,Chemistry ,Cell Membrane ,Mammary Neoplasms, Experimental ,Prodrug ,021001 nanoscience & nanotechnology ,humanities ,0104 chemical sciences ,medicine.anatomical_structure ,Photochemotherapy ,Cancer research ,Female ,0210 nano-technology ,Azo Compounds ,Research Paper ,HeLa Cells - Abstract
Photodynamic therapy (PDT) has emerged as one of the most up-and-coming non-invasive therapeutic modalities for cancer therapy in rencent years. However, its therapeutic effect was still hampered by the short life span, limited diffusion distance and ineluctable depletion of singlet oxygen (1O2), as well as the hypoxic microenvironment in the tumor tissue. Such problems have limited the application of PDT and appropriate solutions are highly demand. Methods: Herein, a programmatic treatment strategy is proposed for the development of a smart molecular prodrug (D-bpy), which comprise a two-photon photosensitizer and a hypoxia-activated chemotherapeutic prodrug. A rhodamine dye was designed to connect them and track the drug release by the fluorescent signal generated through azo bond cleavage. Results: The prodrug (D-bpy) can stay on the cell membrane and enrich at the tumor site. Upon light irradiation, the therapeutic effect was enhanced by a stepwise treatment: (i) direct generation of 1O2 on the cell membrane induced membrane destruction and promoted the D-bpy uptake; (ii) deep tumor hypoxia caused by two-photon PDT process further triggered the activation of the chemotherapy prodrug. Both in vitro and in vivo experiments, D-bpy have exhabited excellent tumor treatment effect. Conclusion: The innovative programmatic treatment strategy provides new strategy for the design of follow-up anticancer drugs.
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- 2021
18. Study on host-guest interaction of aroma compounds/γ-cyclodextrin inclusion complexes
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Xingran Kou, Yaqi Zhang, Dongdong Su, Hui Wang, Xin Huang, Yunwei Niu, Qinfei Ke, Zuobing Xiao, and Qingran Meng
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Food Science - Published
- 2023
19. Peptide and protein modified metal clusters for cancer diagnostics
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Xueyun Gao, Dongdong Su, Xiangchun Zhang, Fuping Gao, and Liang Gao
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Chemistry ,Low toxicity ,Computer science ,Nanotechnology ,General Chemistry ,Materials design ,Metal clusters - Abstract
A perspective on the development of peptide and protein modified metal clusters in cancer diagnostic applications., The biomedical features of metal clusters have been explored in tumor diagnostic applications in recent years. Peptide or protein protected metal clusters with low toxicity, ultra-small size and good biocompatibility are ideal bioanalytical tools, and exhibit better cancer diagnostic properties that have been attractive to oncologists. This perspective provides a rigorous but succinct overview of cancer diagnosis as a working concept for metal clusters by reporting the latest significant advances in the applications of metal clusters in tumor-related bioanalysis and diagnosis. The materials design principles, bioanalytical mechanisms and biomedical applications of metal clusters are described, and then the potential challenges and prospects of metal clusters in cancer diagnosis are discussed. A perspective addressing the role of metal clusters in this field is required to understand their effects and functions, as well as for the scientific community to further advance the development of metal clusters for broader diagnostic applications.
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- 2020
20. Noble-metal nanocluster as enzyme-mimetic catalyst for diagnostic analysis
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Dongdong Su, Qing Yuan, ShiZheng Wang, Xueyun Gao, Liang Gao, and Xiaojun Ren
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chemistry.chemical_classification ,Enzyme ,chemistry ,Diagnostic analysis ,General Engineering ,engineering ,General Materials Science ,Noble metal ,engineering.material ,Combinatorial chemistry ,Catalysis - Published
- 2019
21. Effects of seedling age and root pruning on root characteristics and dry matter accumulation dynamics in machine-transplanted rice
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Tingjue Wang, Wei Xiong, Fuming Kuang, Dongdong Sun, Zixuan Geng, Jinnan Que, Ruize Hou, and Dequan Zhu
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agronomic productivity ,plant physiology ,rice yield optimisation ,stress adaptation ,transplantation techniques ,Plant culture ,SB1-1110 - Abstract
To investigate the impact of different seedling transplanting times on rice growth, the present study evaluated seedling age and root pruning using treatments consisting of root pruning (RC20, RC30, and RC40) and no root pruning (CK20, CK30, and CK40). Quantitative analysis using curve fitting of the changes in dry matter accumulation (DMA) during the seedling and field stages was performed, and the changes in root parameters during the re-greening stage were observed. The results showed that the seedling stage could be divided into a gradual increase period, a rapid increase period, and a slow increase period. Transplanting at different time periods resulted in different transplanting shock effects. During the field stage, the DMA exhibited a slow-fast-slow "S" shaped curve as the developmental time progressed. However, significant differences were observed in growth parameters among the different treatments. Root injury promoted early maturity in young seedlings but also prolonged the whole growth period in older seedlings. The inhibitory effect of root pruning on rice root growth increased with young seedling age. The present results provide a theoretical basis for the design of seedling needle structure and the optimisation of rice seedling cultivation practices.
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- 2024
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22. α‐Hederin induces paraptosis by targeting GPCRs to activate Ca2+/MAPK signaling pathway in colorectal cancer
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Xiwu Rao, Ziwen Li, Qinchang Zhang, Yueyang Lai, Jianrong Liu, Liu Li, Haibo Cheng, Weixing Shen, and Dongdong Sun
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calcium ,colorectal cancer ,MAPK ,paraptosis ,α‐Hederin ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Non‐apoptotic cell death is presently emerging as a potential direction to overcome the apoptosis resistance of cancer cells. In the current study, a natural plant agent α‐hederin (α‐hed) induces caspase‐independent paraptotic modes of cell death. Purpose The present study is aimed to investigate the role of α‐hed induces paraptosis and the associated mechanism of it. Methods The cell proliferation was detected by CCK‐8. The cytoplasm organelles were observed under electron microscope. Calcium (Ca2+) level was detected by flow cytometry. Swiss Target Prediction tool analyzed the potential molecule targets of α‐hed. Molecular docking methods were used to evaluate binding abilities of α‐hed with targets. The expressions of genes and proteins were analyzed by RT‐qPCR, western blotting, immunofluorescence, and immunohistochemistry. Xenograft models in nude mice were established to evaluate the anticancer effects in vivo. Results α‐hed exerted significant cytotoxicity against a panel of CRC cell lines by inhibiting proliferation. Besides, it induced cytoplasmic vacuolation in all CRC cells. Electron microscopy images showed the aberrant dilation of endoplasmic reticulum and mitochondria. Both mRNA and protein expressions of Alg‐2 interacting proteinX (Alix), the marker of paraptosis, were inhibited by α‐hed. Besides, both Swiss prediction and molecular docking showed that the structure of α‐hed could tightly target to GPCRs. GPCRs were reported to activate the phospholipase C (PLC)‐β3/ inositol 1,4,5‐trisphosphate receptor (IP3R)/ Ca2+/ protein kinase C alpha (PKCα) pathway, and we then found all proteins and mRNA expressions of PLCβ3, IP3R, and PKCα were increased by α‐hed. After blocking the GPCR signaling, α‐hed could not elevate Ca2+ level and showed less CRC cell cytotoxicity. MAPK cascade is the symbol of paraptosis, and we then demonstrated that α‐hed activated MAPK cascade by elevating Ca2+ flux. Since non‐apoptotic cell death is presently emerging as a potential direction to overcome chemo‐drug resistance, we then found α‐hed also induced paraptosis in 5‐fluorouracil‐resistant (5‐FU‐R) CRC cells, and it reduced the growth of 5‐FU‐R CRC xenografts. Conclusions Collectively, our findings proved α‐hed as a promising candidate for inducing non‐apoptotic cell death, paraptosis. It may overcome the resistance of apoptotic‐based chemo‐resistance in CRC.
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- 2024
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23. Effect of electroacupuncture at Zusanli (ST 36) on dorsal root nerve signals in normal rats
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Dongdong, Su, Lianqi, Geng, Jingsha, Zhang, Yingyuan, Chen, Yangyang, Liu, Lijuan, Yan, and Yi, Guo
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Rats, Sprague-Dawley ,Electroacupuncture ,Animals ,Female ,Rats, Wistar ,Spinal Nerve Roots ,Rats - Abstract
To investigate how electroacupuncture (EA) at Zusanli (ST 36) with varying intensities of stimulation affects dorsal root nerve signals in normal rats.Adult female Wistar rats were examined after drug-induced anesthesia and isolation of the L4 dorsal root associated with the Zusanli (ST 36) acupoint, using bipolar platinum electrodes. We applied EA at Zusanli (ST 36) with a continuous wave waveform for 1 min, and recorded action potentials in the L4 spinal cord dorsal root nerve with a frequency of 5 Hz and various current intensity levels (approximately 1-4 mA). In addition, we tested EA with a frequency of 15 Hz and current intensity of 1-4 mA. The L4 spinal cord dorsal nerve bundle action potentials were measured for 1 min of EA. To analyze the discharge frequency, we used the maximum Lyapunov exponent and Lempel-Ziv (LZ) complexity.At a fixed frequency, with increasing intensity of EA, dorsal root nerve filament discharge frequency revealed an initial increase, followed by a decreasing trend. A stimulation intensity of 3 mA induced a significantly greater discharge frequency, compared with stimulation intensities of 2 and 4 mA. EA stimulation evoked neuroelectric signals with chaotic characteristics. Increased intensity led to an initial increase in LZ complexity which then decreased, with a stimulation strength of 3 mA inducing the highest level of LZ complexity.EA of different intensities can induce nerve action potential encoding with different features.
- Published
- 2020
24. Visualization of oxidative injury in the mouse kidney using selective superoxide anion fluorescent probes† †Electronic supplementary information (ESI) available: Experimental procedures, characterization data, and additional spectra. See DOI: 10.1039/c8sc03308k
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Lv, Yun, Dan Cheng, Dongdong Su, Chen, Mei, Yin, Bin-Cheng, Yuan, Lin, and Zhang, Xiao-Bing
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Chemistry ,urogenital system ,urologic and male genital diseases ,female genital diseases and pregnancy complications - Abstract
Drug-induced acute kidney injury (AKI), caused by renal drug metabolism, has been regarded as a main problem in clinical pharmacology and practice., Drug-induced acute kidney injury (AKI), caused by renal drug metabolism, has been regarded as a main problem in clinical pharmacology and practice. However, due to the lack of effective biomarkers and noninvasive real-time tools, the early diagnosis of drug-induced AKI is still a crucial challenge. The superoxide anion (O2˙–), the preliminary reactive oxidative species, is closely related to drug-induced AKI. In this paper, we reported two new mitochondria-targeted fluorescent probes for investigating AKI via mapping the fluctuation of O2˙– with high sensitivity and selectivity by the combination of rational design and a probe-screening approach. Small-molecule fluorescent probes (Naph-O2˙– and NIR-O2˙–) with high accuracy and excellent selectivity were successfully applied to detect endogenously produced O2˙– in living cells and tissues by dual-model confocal imaging, and to trap the fluctuation of the O2˙– level during the drug-induced nephrotoxicity. Moreover, probe NIR-O2˙– was also used to elucidate the protective effects of l-carnitine (LC) against drug-induced nephrotoxicity for the first time. Therefore, these probes may be potential chemical tools for exploring the roles of O2˙– in complex nephrotoxicity disease systems.
- Published
- 2018
25. Seeing Elastin: A Near-Infrared Zwitterionic Fluorescent Probe for In Vivo Elastin Imaging
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Jong-Jin Kim, Sungjin Park, Chai Lean Teoh, Myung Ae Bae, Renzhe Bi, U. S. Dinish, Michiya Matsusaki, Seong Soon Kim, Young-Tae Chang, Animesh Samanta, Malini Olivo, Marie Piantino, Dongdong Su, and Fiona Louis
- Subjects
0301 basic medicine ,Optical contrast ,biology ,Wavelength range ,Chemistry ,General Chemical Engineering ,Biochemistry (medical) ,Near-infrared spectroscopy ,Photoacoustic imaging in biomedicine ,General Chemistry ,Biochemistry ,Fluorescence ,Imaging modalities ,03 medical and health sciences ,030104 developmental biology ,In vivo ,Materials Chemistry ,biology.protein ,Biophysics ,Environmental Chemistry ,Elastin - Abstract
Summary Elastic fibers are present in a variety of tissues and are responsible for their resilience. Until now, no optical contrast agent in the near-infrared (NIR) wavelength range of 700–900 nm has been reported for the imaging of elastic fibers. Here, we report the discovery of a NIR zwitterionic elastin probe ElaNIR (elastin NIR) through fluorescent-image-based screening. The probe was successfully applied for in vitro , ex vivo , and in vivo imaging by various imaging modalities. Age-related elastin differences shown by in vivo fluorescent and photoacoustic imaging indicated that ElaNIR can be a potentially convenient tool for uncovering changes of elastin in live models.
- Published
- 2018
26. Visualization of oxidative injury in the mouse kidney using selective superoxide anion fluorescent probes
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Bin-Cheng Yin, Yun Lv, Xiao-Bing Zhang, Dongdong Su, Mei Chen, Lin Yuan, and Dan Cheng
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chemistry.chemical_classification ,Reactive oxygen species ,010405 organic chemistry ,Chemistry ,Superoxide ,Rational design ,Acute kidney injury ,General Chemistry ,010402 general chemistry ,medicine.disease ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,Nephrotoxicity ,chemistry.chemical_compound ,Biochemistry ,medicine ,Mouse Kidney ,Oxidative injury - Abstract
Drug-induced acute kidney injury (AKI), caused by renal drug metabolism, has been regarded as a main problem in clinical pharmacology and practice. However, due to the lack of effective biomarkers and noninvasive real-time tools, the early diagnosis of drug-induced AKI is still a crucial challenge. The superoxide anion (O2˙-), the preliminary reactive oxidative species, is closely related to drug-induced AKI. In this paper, we reported two new mitochondria-targeted fluorescent probes for investigating AKI via mapping the fluctuation of O2˙- with high sensitivity and selectivity by the combination of rational design and a probe-screening approach. Small-molecule fluorescent probes (Naph-O2˙- and NIR-O2˙- ) with high accuracy and excellent selectivity were successfully applied to detect endogenously produced O2˙- in living cells and tissues by dual-model confocal imaging, and to trap the fluctuation of the O2˙- level during the drug-induced nephrotoxicity. Moreover, probe NIR-O2˙- was also used to elucidate the protective effects of l-carnitine (LC) against drug-induced nephrotoxicity for the first time. Therefore, these probes may be potential chemical tools for exploring the roles of O2˙- in complex nephrotoxicity disease systems.
- Published
- 2018
27. Detection of analytes in mitochondria without interference from other sites based on an innovative ratiometric fluorophore
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Tian-Bing Ren, Dongdong Su, Xiao-Bing Zhang, Xing-Xing Zhang, Qian-Ling Zhang, and Lin Yuan
- Subjects
Analyte ,Fluorophore ,010405 organic chemistry ,General Chemistry ,Mitochondrion ,010402 general chemistry ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,Chemistry ,chemistry.chemical_compound ,Target site ,chemistry ,Interference (communication) ,Mitochondrial targeting ,Biophysics ,Reaction site - Abstract
A new strategy that integrates the targeting group and response moiety together for the preparation of mitochondrial probe was developed. Bioimaging studies have shown that for the first time, the newly designed probe HDFL-Cys can first accumulate in mitochondria and then react with the analyte., Mitochondria are vital organelles that not only produce cellular energy but also participate in many biological processes. Recently, various fluorescent probes have been developed for mitochondrial imaging. However, due to the lack of suitable dyes or strategies, it is difficult for most reported mitochondrial targeting probes to prove whether the analytes they detected are from mitochondria. In addition, positive charge on mitochondrial probes can seriously affect the mitochondrial environment. To address these issues, we herein put forward a novel strategy for probe design based on a smart NIR dye (HDFL) for mitochondrial targeting detection. Compared to general mitochondrial targeting probes that are modified with a target site and a reaction site, the new strategy is to combine the two sites together for a mitochondrial probe that would provide accurate detection of analytes in mitochondria without interference. As a proof of concept, we synthesized a mitochondrial-targetable probe HDFL-Cys for cysteine. Bioimaging studies have shown that the new type of probe HDFL-Cys can first accumulate in mitochondria and then react with the analyte (cysteine) accompanied by the departure of the targeting group (lipophilic cation moieties). Thus, it can specifically detect the analyte in mitochondria without interference from extra-mitochondrial analytes. We anticipate that the new strategy based on the novel NIR dye HDFL may be a potential platform for developing desirable ratiometric fluorescent probes for mitochondrial imaging.
- Published
- 2018
28. A palette of background-free tame fluorescent probes for intracellular multi-color labelling in live cells
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Hee-Sung Park, Kyung Jin Lee, José L. Belmonte-Vázquez, Samira Husen Alamudi, Young-Tae Chang, Jung Yeol Lee, Eduardo Peña-Cabrera, and Dongdong Su
- Subjects
chemistry.chemical_classification ,Bioconjugation ,010405 organic chemistry ,Chemistry ,Biomolecule ,General Chemistry ,010402 general chemistry ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,chemistry.chemical_compound ,Labelling ,Biophysics ,Click chemistry ,Azide ,BODIPY ,Intracellular - Abstract
A multi-color labelling technique for visualizing multiple intracellular apparatuses in their native environment using small fluorescent probes remains challenging. This approach requires both orthogonal and biocompatible coupling reactions in heterogeneous biological systems with minimum fluorescence background noise. Here, we present a palette of BODIPY probes containing azide and cyclooctyne moieties for copper-free click chemistry in living cells. The probes, referred to as ‘tame probes’, are highly permeable and specific in nature, leaving no background noise in cells. Such probes, which are rationally designed through optimized lipophilicity, water solubility and charged van der Waals surface area, allow us to demonstrate rapid and efficient concurrent multi-labelling of intracellular target components. We show that these probes are capable of not only labelling organelles and engineered proteins, but also showing the intracellular glycoconjugates’ dynamics, through the use of metabolic oligosaccharide engineering technology in various cell types. The results demonstrated in this study thus provide flexibility for multi-spectral labelling strategies in native systems in a high spatiotemporal manner.
- Published
- 2018
29. Optimal Cooperative Wireless Communication for Mobile User Data Offloading
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Hongwu Lv, Haibin Lv, Huiqiang Wang, Dongdong Su, Fumin Xia, Guangsheng Feng, and Yongmin Zhang
- Subjects
Optimization problem ,Mobile data offloading ,General Computer Science ,Distributed database ,Computer science ,business.industry ,General Engineering ,Mobile computing ,020206 networking & telecommunications ,bi-level optimization ,utility function ,02 engineering and technology ,cooperative transmission ,Base station ,user satisfaction ,0202 electrical engineering, electronic engineering, information engineering ,Cellular network ,Wireless ,020201 artificial intelligence & image processing ,General Materials Science ,lcsh:Electrical engineering. Electronics. Nuclear engineering ,business ,lcsh:TK1-9971 ,Computer network - Abstract
We study the mobile data offloading problem in cooperative cellular networks, in which the cellular base station can offload a fraction of data traffic to its cooperative Wi-Fi networks. Both user satisfaction and benefit are important for the cellular network. Under the case that the cellular network cannot provide sufficient transmission resources, part of data traffic can be offloaded to its cooperative Wi-Fi networks to guarantee the user satisfaction, even though the benefit loss of the cellular network will be incurred. Meanwhile, considering the users are price sensitive, the operators, including cellular and Wi-Fi, can issue an appreciate unit price to attract more users and more traffic requirements to increase their total benefit. In this paper, we intend to design an optimal pricing scheme to maximize the user satisfaction and minimize the benefit losses of the operators simultaneously. First, we formulate the mobile data offloading problem as a joint optimization of “min–max” problem. Then, we convert the problem into a bi-level optimization problem, in which the lower level is to maximize the user satisfaction and the upper level is to minimize the benefit losses of the operators. Since the lower-level problem is convex, a centralized scheme based on the dynamic coordinate search-based method is employed to find the optimal solution of the bi-level problem. In addition, a distributed method is proposed to obtain the near-optimal solution quickly, where one-third-party agent is used to adjust the data traffic allocation. Simulation results show that the proposed method achieves the near-optimal solutions with limited iterations.
- Published
- 2018
30. Sirt6 enhances macrophage lipophagy and improves lipid metabolism disorder by regulating the Wnt1/β-catenin pathway in atherosclerosis
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Tingting Wang, Zheng Cheng, Ran Zhao, Jin Cheng, He Ren, Pengke Zhang, Pengyun Liu, Qimeng Hao, Qian Zhang, Xiaolei Yu, Dongdong Sun, and Dongwei Zhang
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Atherosclerosis ,Lipid metabolism ,Macrophage lipophagy ,Foam cells ,Sirt6 ,Nutritional diseases. Deficiency diseases ,RC620-627 - Abstract
Abstract Lipid metabolism disorders are considerably involved in the pathology of atherosclerosis; nevertheless, the fundamental mechanism is still largely unclear. This research sought to examine the function of lipophagy in lipid metabolism disorder-induced atherosclerosis and its fundamental mechanisms. Previously, Sirt6 has been reported to stimulate plaque stability by promoting macrophage autophagy. However, its role in macrophage lipophagy and its relationship with Wnt1 remains to be established. In this study, ApoE−/−: Sirt6−/− and ApoE−/−: Sirt6Tg mice were used and lipid droplets were analysed via transmission electron microscopy and Bodipy 493/503 staining in vitro. Atherosclerotic plaques in ApoE−/−: Sirt6−/− mice showed greater necrotic cores and lower stability score. Reconstitution of Sirt6 in atherosclerotic mice improved lipid metabolism disorder and prevented the progression of atherosclerosis. Furthermore, macrophages with Ac-LDL intervention showed more lipid droplets and increased expression of adipophilin and PLIN2. Reconstitution of Sirt6 recruited using SNF2H suppressed Wnt1 expression and improved lipid metabolism disorder by promoting lipophagy. In addition, downregulation of Sirt6 expression in Ac-LDL-treated macrophages inhibited lipid droplet degradation and stimulated foam cell formation. Innovative discoveries in the research revealed that atherosclerosis is caused by lipid metabolism disorders due to downregulated Sirt6 expression. Thus, modulating Sirt6’s function in lipid metabolism might be a useful therapeutic approach for treating atherosclerosis.
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- 2023
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31. The host–guest inclusion driven by host-stabilized charge transfer for construction of sequentially red-shifted mechanochromic system
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Dongdong Sun, Yong Wu, Xie Han, and Simin Liu
- Subjects
Science - Abstract
Abstract Developing more extensive methods to understand the underlying structure-property relationship of mechanochromic luminescent molecules is demanding but remains challenging. Herein, the effect of host-guest interaction on the mechanochromic properties of organic molecules is illustrated. A series of pyridinium-functionalized triphenylamine derivatives show bathochromic-shifted emission upon mechanical stimulation. These derivatives bind to cucurbit[8]uril to form homoternary host-guest inclusion complexes through host-stabilized intermolecular charge transfer interactions. Remarkably, the homoternary complexes exhibit longer emission than that of free guests in the solid state (even longer than ground guests), and a further bathochromic-shifted emission is observed upon grinding. Additionally, a heteroternary complex constructed through the encapsulation of pyrene (donor) and pyridinium (acceptor) guest pair in cucurbit[8]uril also displays the mechanochromic luminescent property. This work not only discloses the effect of host-guest inclusion on the mechanochromic property of organic molecules, but also provides a principle and a facile way to design the sequentially red-shifted mechanochromic materials.
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- 2023
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32. A new approach for turn-on fluorescence sensing of l-DOPA
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Young-Tae Chang, Jungyeol Lee, Lu Wang, Dongdong Su, and Stuart N. Berry
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Chemistry ,Metals and Alloys ,Analytical chemistry ,Fluorescence sensing ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Catalysis ,nervous system diseases ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Turn (biochemistry) ,parasitic diseases ,Materials Chemistry ,Ceramics and Composites ,Biophysics ,0210 nano-technology ,Fluorescence response - Abstract
A novel design strategy for the fluorescence sensing of l-DOPA is reported. Resa-Sulf displays a significant turn-on fluorescence response to l-DOPA due to its reduction properties; this sensing mechanism was fully confirmed by mechanistic studies. Furthermore, Resa-Sulf was successfully utilized to quantitatively detect l-DOPA concentrations from a commercially available source.
- Published
- 2017
33. Enzyme-activated near-infrared fluorogenic probe with high-efficiency intrahepatic targeting ability for visualization of drug-induced liver injury.
- Author
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Yong Zhang, Xueqian Chen, Qing Yuan, Yongning Bian, Mingrui Li, Yaling Wang, Xueyun Gao, and Dongdong Su
- Published
- 2021
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34. An ESIPT-Based Ratiometric Fluorescent Probe for Highly Sensitive and Rapid Detection of Sulfite in Living Cells
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Jianing Hou, Dan Cheng, Yu-Peng Liu, Tian-Bing Ren, Dongdong Su, and Lin Yuan
- Subjects
Detection limit ,Fluorescence-lifetime imaging microscopy ,Full Paper ,010405 organic chemistry ,General Chemistry ,Full Papers ,010402 general chemistry ,Photochemistry ,Condensation reaction ,01 natural sciences ,Fluorescence ,Rapid detection ,0104 chemical sciences ,Highly sensitive ,lcsh:Chemistry ,chemistry.chemical_compound ,sulfite ,Sulfite ,chemistry ,lcsh:QD1-999 ,imaging studies ,fluorescent probe ,Michael reaction ,fluorescence ,ratiometric probes - Abstract
The novel ratiometric fluorescent probe HPQRB with an ESIPT effect based on Michael addition for highly sensitive and fast detection of sulfite in living HepG2 cells is reported. HPQRB can be easily synthesized by a two‐step condensation reaction. HPQRB has a large emission shift (Δλ=116 nm), which is beneficial for fluorescence imaging research, and its sulfite‐responsive site is based on a rhodamine‐like structure with the emission peak at 566 nm, which decreases with increasing sulfite concentration. and its HPQ structure always has an ESIPT effect throughout the reaction process, keeping the emission peak at 450 nm as a self‐reference. In particular, HPQRB has high selectivity for sulfite and responds quickly (within 30 s) with a low detection limit (44 nM). Furthermore, HPQRB has been successfully used for fluorescence imaging of sulfite in HepG2 cells, demonstrating the superior ability to detect sulfite under physiological conditions.
- Published
- 2019
35. Frontispiece: Visualizing Microglia with a Fluorescence Turn‐On Ugt1a7c Substrate
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Audrey Tze Ting Khoo, Jungyeol Lee, Aymeric Silvin, H. Shawn Je, Beomsue Kim, Sungjin Park, Xiaogang Liu, Taejoon Kwon, Masahiro Fukuda, Xiao Liu, Weijie Chi, Dongdong Su, Sejong Choi, Diana S. Y. Wan, Florent Ginhoux, Srikanta Sanu, Young-Tae Chang, and Jin-Soo Kim
- Subjects
Turn (biochemistry) ,medicine.anatomical_structure ,Microglia ,Chemistry ,Biophysics ,medicine ,Substrate (chemistry) ,General Chemistry ,Fluorescence ,Catalysis - Published
- 2019
36. Frontispiz: Visualizing Microglia with a Fluorescence Turn‐On Ugt1a7c Substrate
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Beomsue Kim, Masahiro Fukuda, Jung‐Yeol Lee, Dongdong Su, Srikanta Sanu, Aymeric Silvin, Audrey T. T. Khoo, Taejoon Kwon, Xiao Liu, Weijie Chi, Xiaogang Liu, Sejong Choi, Diana S. Y. Wan, Sung‐Jin Park, Jin‐Soo Kim, Florent Ginhoux, H. Shawn Je, and Young‐Tae Chang
- Subjects
General Medicine - Published
- 2019
37. Identification and Functional Characterization of an Odorant Receptor Expressed in the Genitalia of Helicoverpa armigera
- Author
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Weihao Liu, Dongdong Sun, Xiaoqing Wang, Zhiqiang Wang, and Yang Liu
- Subjects
Helicoverpa armigera ,genitalia ,sensilla ,olfactory receptor ,scanning electron micrograph ,Agriculture (General) ,S1-972 - Abstract
Olfaction is critical for guiding the physiological activities of insects, with antennae being the primary olfactory organs. However, recent evidence suggests that other tissues may also participate in olfactory recognition. Among these, the genitalia of moths have received attention due to their roles in mating and oviposition. Sensilla and odorant receptors (ORs) in moth genitalia highlight the potential olfactory function of these structures. In this study, we examined the olfactory sensing capacity of the genitalia in Helicoverpa armigera by analyzing their structure in males and females and characterizing the expressed ORs. Scanning electron microscopy uncovered many sensilla distributed throughout the male and female genitalia. Transcriptome sequencing identified 20 ORs in the genitalia, with HarmOR68 exhibiting significant responses to methyl esters: methyl benzoate and salicylate. Our findings provide theoretical evidence that H. armigera genitalia may have significant olfactory perception functions.
- Published
- 2024
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38. Adipsin alleviates cardiac microvascular injury in diabetic cardiomyopathy through Csk-dependent signaling mechanism
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Xuebin Zhang, Yu Duan, Xiao Zhang, Mengyuan Jiang, Wanrong Man, Yan Zhang, Dexi Wu, Jiye Zhang, Xinglong Song, Congye Li, Jie Lin, and Dongdong Sun
- Subjects
Diabetic cardiomyopathy ,Adipsin ,Exosomes ,Adherens junctions ,Permeability ,Medicine - Abstract
Abstract Background Microvascular complications are associated with an overtly increased risk of adverse outcomes in patients with diabetes including coronary microvascular injury which manifested as disruption of adherens junctions between cardiac microvascular endothelial cells (CMECs). However, particular mechanism leading to diabetic coronary microvascular hyperpermeability remains elusive. Methods Experimental diabetes was induced in mice with adipose tissue-specific Adipsin overexpression (AdipsinLSL/LSL-Cre) and their respective control (AdipsinLSL/LSL). In addition, cultured CMECs were subjected to high glucose/palmitic acid (HG + PA) treatment to simulate diabetes for a mechanistic approach. Results The results showed that Adipsin overexpression significantly reduced cardiac microvascular permeability, preserved coronary microvascular integrity, and increased coronary microvascular density. Adipsin overexpression also attenuated cardiac dysfunction in diabetic mice. E/A ratio, an indicator of cardiac diastolic function, was improved by Adipsin. Adipsin overexpression retarded left ventricular adverse remodeling, enhanced LVEF, and improved cardiac systolic function. Adipsin-enriched exosomes were taken up by CMECs, inhibited CMECs apoptosis, and increased CMECs proliferation under HG + PA treatment. Adipsin-enriched exosomes also accelerated wound healing, rescued cell migration defects, and promoted tube formation in response to HG + PA challenge. Furthermore, Adipsin-enriched exosomes maintained adherens junctions at endothelial cell borders and reversed endothelial hyperpermeability disrupted by HG + PA insult. Mechanistically, Adipsin blocked HG + PA-induced Src phosphorylation (Tyr416), VE-cadherin phosphorylation (Tyr685 and Tyr731), and VE-cadherin internalization, thus maintaining CMECs adherens junctions integrity. LC-MS/MS analysis and co-immunoprecipitation analysis (Co-IP) unveiled Csk as a direct downstream regulator of Adipsin. Csk knockdown increased Src phosphorylation (Tyr416) and VE-cadherin phosphorylation (Tyr685 and Tyr731), while abolishing Adipsin-induced inhibition of VE-cadherin internalization. Furthermore, Csk knockdown counteracted Adipsin-induced protective effects on endothelial hyperpermeability in vitro and endothelial barrier integrity of coronary microvessels in vivo. Conclusions Together, these findings favor the vital role of Adipsin in the regulation of CMECs adherens junctions integrity, revealing its promises as a treatment target against diabetic coronary microvascular dysfunction. Graphical Abstract Graphical abstract depicting the mechanisms of action behind Adipsin-induced regulation of diabetic coronary microvascular dysfunction.
- Published
- 2023
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39. Correction: Andrographolide inhibits breast cancer through suppressing COX-2 expression and angiogenesis via inactivation of p300 signaling and VEGF pathway
- Author
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Yulin Peng, Yan Wang, Ning Tang, Dongdong Sun, Yulong Lan, Zhenlong Yu, Xinyu Zhao, Lei Feng, Baojing Zhang, Lingling Jin, Fabiao Yu, Xiaochi Ma, and Chuanzhu Lv
- Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Published
- 2023
- Full Text
- View/download PDF
40. Chemical Fluorescent Probe for Detection of Aβ Oligomers
- Author
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Srikanta Sahu, Young-Tae Chang, Seong-Wook Yun, Sunhee Cho, Dongdong Su, Sihyun Ham, Frances Prelli, Chai Lean Teoh, Eleanor Drummond, Sulgi Lim, and Thomas Wisniewski
- Subjects
Models, Molecular ,Amyloid ,Fibril ,Biochemistry ,Oligomer ,Article ,Catalysis ,Mice ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,In vivo ,medicine ,Animals ,Fluorescent Dyes ,Amyloid beta-Peptides ,Chemistry ,Neurodegeneration ,Brain ,Fibrillogenesis ,General Chemistry ,medicine.disease ,Fluorescence ,In vitro ,Disease Models, Animal ,Thermodynamics - Abstract
Aggregation of amyloid β-peptide (Aβ) is implicated in the pathology of Alzheimer’s disease (AD), with the soluble, Aβ oligomeric species thought to be the critical pathological species. Identification and characterization of intermediate species formed during the aggregation process is crucial to the understanding of the mechanisms by which oligomeric species mediate neuronal toxicity and following disease progression. Probing these species proved to be extremely challenging, as evident by the lack of reliable sensors, due to their heterogeneous and transient nature. We describe here an oligomer-specific fluorescent chemical probe, BoDipy-Oligomer (BD-Oligo), developed through the use of the diversity-oriented fluorescent library approach (DOFLA) and high-content, imaging-based screening. This probe enables dynamic oligomer monitoring during fibrillogenesis in vitro and shows in vivo Aβ oligomers staining possibility in the AD mice model.
- Published
- 2015
41. A mitochondria-targeted ratiometric fluorescent probe to monitor endogenously generated sulfur dioxide derivatives in living cells
- Author
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Juanjuan Peng, Wang Xu, Dongdong Su, Lin Yuan, Chai Lean Teoh, and Young-Tae Chang
- Subjects
Indoles ,Biophysics ,Bioengineering ,Biosensing Techniques ,Mitochondrion ,complex mixtures ,Adduct ,Biomaterials ,HeLa ,chemistry.chemical_compound ,Sulfite ,Coumarins ,Cations ,Humans ,Sulfites ,Sulfur Dioxide ,Hydrogen Sulfide ,Fluorescent Dyes ,chemistry.chemical_classification ,biology ,biology.organism_classification ,Fluorescence ,Mitochondria ,respiratory tract diseases ,Amino acid ,Enzyme ,Microscopy, Fluorescence ,chemistry ,Biochemistry ,Mechanics of Materials ,Ceramics and Composites ,Intracellular ,HeLa Cells - Abstract
Sulfur dioxide (SO2) can be endogenously produced by enzymes in mitochondria during oxidation of H2S or sulphur-containing amino acids, and plays important roles in several physiological processes. However, the design and synthesis of fluorescent probes which can detect mitochondrial SO2 and its derivatives in living cells still remain unresolved. Herein, we report the preparation of a lipophilic cationic dye 1 (Mito-Ratio-SO2), which targets the mitochondria in living cells and is sensitive to the presence of SO2 derivatives. The ratiometric probe Mito-Ratio-SO2 displays a 170 nm blue-shift in emission with two well-resolved emission bands upon addition of sulfite. Mechanistic studies indicate that three probe-SO2 adducts coexist after reaction, as supported by liquid chromatography and density function theory investigations. Importantly, the ratiometric probe is highly selective for sulfite over other bio-species including H2S. Fluorescence co-localization studies indicate that the probe localizes solely in the mitochondria of HeLa cells. Last but not least, fluorescent imaging of HeLa cells successfully demonstrates the detection of intrinsically generated intracellular SO2 derivatives in living cells.
- Published
- 2015
42. Rnd3 suppresses endothelial cell pyroptosis in atherosclerosis through regulation of ubiquitination of TRAF6
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Yan Zhang, Zhengru Zhu, Yang Cao, Zhenyu Xiong, Yu Duan, Jie Lin, Xuebin Zhang, Mengyuan Jiang, Yue Liu, Wanrong Man, Tengfei Jia, Jiaxu Feng, Yanyan Chen, Congye Li, Baolin Guo, and Dongdong Sun
- Subjects
atherosclerosis ,endothelial cell ,pyroptosis ,Rho‐GTPase ,ubiquitination ,Medicine (General) ,R5-920 - Abstract
Abstract Background As the main pathological basis for various cardiovascular and cerebrovascular diseases, atherosclerosis has become one of the leading causes of death and disability worldwide. Emerging evidence has suggested that Rho GTPase Rnd3 plays an indisputable role in cardiovascular diseases, although its function in atherosclerosis remains unclear. Here, we found a significant correlation between Rnd3 and pyroptosis of aortic endothelial cells (ECs). Methods ApoeKO mice were utilized as a model for atherosclerosis. Endothelium‐specific transgenic mice were employed to disrupt the expression level of Rnd3 in vivo. Mechanistic investigation of the impact of Rnd3 on endothelial cell pyroptosis was carried out using liquid chromatography tandem mass spectrometry (LC‐MS/MS), co‐immunoprecipitation (Co‐IP) assays, and molecular docking. Results Evidence from gain‐of‐function and loss‐of‐function studies denoted a protective role for Rnd3 against ECs pyroptosis. Downregulation of Rnd3 sensitized ECs to pyroptosis under oxidized low density lipoprotein (oxLDL) challenge and exacerbated atherosclerosis, while overexpression of Rnd3 effectively prevented these effects. LC‐MS/MS, Co‐IP assay, and molecular docking revealed that Rnd3 negatively regulated pyroptosis signaling by direct interaction with the ring finger domain of tumor necrosis factor receptor‐associated factor 6 (TRAF6). This leads to the suppression of K63‐linked TRAF6 ubiquitination and the promotion of K48‐linked TRAF6 ubiquitination, inhibiting the activation of NF‐κB and promoting the degradation of TRAF6. Moreover, TRAF6 knockdown countered Rnd3 knockout‐evoked exacerbation of EC pyroptosis in vivo and vitro. Conclusions These findings establish a critical functional connection between Rnd3 and the TRAF6/NF‐κB/NLRP3 signaling pathway in ECs, indicating the essential role of Rnd3 in preventing pyroptosis of ECs.
- Published
- 2023
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43. L‐Arginine‐Loaded Gold Nanocages Ameliorate Myocardial Ischemia/Reperfusion Injury by Promoting Nitric Oxide Production and Maintaining Mitochondrial Function
- Author
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Zekun Wang, Nana Yang, Yajun Hou, Yuqing Li, Chenyang Yin, Endong Yang, Huanhuan Cao, Gaofei Hu, Jing Xue, Jialei Yang, Ziyu Liao, Weiyun Wang, Dongdong Sun, Cundong Fan, and Lemin Zheng
- Subjects
L‐arginine ,mitochondrial function ,myocardial function ,myocardial ischaemia/reperfusion injury ,nitric oxide ,reactive oxygen species ,Science - Abstract
Abstract Cardiovascular disease is the leading cause of death worldwide. Reperfusion therapy is vital to patient survival after a heart attack but can cause myocardial ischemia/reperfusion injury (MI/RI). Nitric oxide (NO) can ameliorate MI/RI and is a key molecule for drug development. However, reactive oxygen species (ROS) can easily oxidize NO to peroxynitrite, which causes secondary cardiomyocyte damage. Herein, L‐arginine‐loaded selenium‐coated gold nanocages (AAS) are designed, synthesized, and modified with PCM (WLSEAGPVVTVRALRGTGSW) to obtain AASP, which targets cardiomyocytes, exhibits increased cellular uptake, and improves photoacoustic imaging in vitro and in vivo. AASP significantly inhibits oxygen glucose deprivation/reoxygenation (OGD/R)‐induced H9C2 cell cytotoxicity and apoptosis. Mechanistic investigation revealed that AASP improves mitochondrial membrane potential (MMP), restores ATP synthase activity, blocks ROS generation, and prevents NO oxidation, and NO blocks ROS release by regulating the closing of the mitochondrial permeability transition pore (mPTP). AASP administration in vivo improves myocardial function, inhibits myocardial apoptosis and fibrosis, and ultimately attenuates MI/RI in rats by maintaining mitochondrial function and regulating NO signaling. AASP shows good safety and biocompatibility in vivo. This findings confirm the rational design of AASP, which can provide effective treatment for MI/RI.
- Published
- 2023
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44. Overview of research progress and application of experimental models of colorectal cancer
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Li Liu, Qiuying Yan, Zihan Chen, Xiaoman Wei, Lin Li, Dongxin Tang, Jiani Tan, Changliang Xu, Chengtao Yu, Yueyang Lai, Minmin Fan, Lihuiping Tao, Weixing Shen, Liu Li, Mianhua Wu, Haibo Cheng, and Dongdong Sun
- Subjects
colorectal cancer ,cellular models ,animal models ,preclinical studies ,drug development ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Colorectal cancer (CRC) is the third most common malignancy in terms of global tumor incidence, and the rates of morbidity and mortality due to CRC are rising. Experimental models of CRC play a vital role in CRC research. Clinical studies aimed at investigating the evolution and mechanism underlying the formation of CRC are based on cellular and animal models with broad applications. The present review classifies the different experimental models used in CRC research, and describes the characteristics and limitations of these models by comparing the research models with the clinical symptoms. The review also discusses the future prospects of developing new experimental models of CRC.
- Published
- 2023
- Full Text
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45. Dark to light! A new strategy for large Stokes shift dyes: coupling of a dark donor with tunable high quantum yield acceptors
- Author
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Juwon Oh, Sung-Chan Lee, Jong Min Lim, Dongdong Su, Xiaotong Yu, Dongho Kim, Srikanta Sahu, and Young-Tae Chang
- Subjects
Chemistry ,Astrophysics::High Energy Astrophysical Phenomena ,Quantum yield ,Resonance ,General Chemistry ,Photochemistry ,Fluorescence ,Acceptor ,symbols.namesake ,chemistry.chemical_compound ,Förster resonance energy transfer ,Stokes shift ,Excited state ,symbols ,Physics::Chemical Physics ,BODIPY - Abstract
A new strategy for constructing large Stokes shift dyes by coupling a low quantum yield (less than 1%) BODIPY donor (BDN) with tunable high quantum yield BODIPY acceptors (BDM) has been explored to synthesize a set of novel Dark Resonance Energy Transfer (DRET) dyes, named BNM. The low quantum yield of the donor is ascribed to the intramolecular rotation of the phenyl rings, which has been proven by controlling the viscosity and temperature of the solvent. However, upon excitation of BNM compounds at the donor absorption wavelength, tunable emissions from 560 nm to 617 nm were obtained, with a high quantum yield of up to 0.75. Ultrafast dynamic studies demonstrated that the absorbed energy was transferred to the acceptor (BDM) with a high energy transfer rate, before being quenched by non-radiative intramolecular rotations. Using a dark donor makes it possible to avoid fluorescence leaks from donor emission. This is a new set of RET dyes that can be excited by a low quantum yield donor to emit a tunable wide range of high fluorescence emission.
- Published
- 2014
46. A novel tRNA-derived fragment tRF-3022b modulates cell apoptosis and M2 macrophage polarization via binding to cytokines in colorectal cancer
- Author
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Sicheng Lu, Xiaoman Wei, Lihuiping Tao, Dan Dong, Wenlong Hu, Qinchang Zhang, Yuquan Tao, Chengtao Yu, Dongdong Sun, and Haibo Cheng
- Subjects
tRNA-derived fragments ,tRF-3022b ,LGALS1 ,MIF ,Colorectal cancer ,Diseases of the blood and blood-forming organs ,RC633-647.5 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract tRNA-derived fragments (tRFs) are a class of small RNAs that occur when tRNAs are broken down by enzymes due to stress. Increasing reports have shown that tRFs are associated with multiple physiological and pathological processes, especially in cancers; however, very little is known of the effects and mechanisms of tRFs. Therefore, further investigation on the biological roles and clinical value of tRFs is required. In this study, we utilized whole-transcriptome sequencing to profile tRFs expression in the tissues and plasma exosomes of patients with colorectal cancer (CRC). Three tRFs (tRF-3022b, tRF-3030b and tRF-5008b) showed an increasing trend in CRC tissues compared to adjacent normal tissues. They also tended to be elevated in plasma exosomes of CRC patients compared to healthy controls. These results indicated that they may be upregulated in cancer cells and then secreted by exosomes. The knockdown of tRF-regulated factors such as AlkB homolog 3 (ALKBH3), tRNA aspartic acid methyltransferase 1 (DNMT2), angiogenin (ANG), and argonaute RISC catalytic component 2 (AGO2) could affect the expression of tRFs. Notably, we found that the decrease in the three tRFs arrests the progression of the CRC cell cycle and induces cell apoptosis. Silencing tRF-3022b could facilitate M2 macrophage polarization. Mechanistically, we found that tRF-3022b binds to galectin 1 (LGALS1) and macrophage migration inhibitory factor (MIF) in CRC cells and reduces polarization by regulating MIF in M2 macrophages. In conclusion, our study revealed the expression pattern of tRFs in both tissue and plasma exosomes and identified a novel tRF, tRF-3022b, which may affect CRC tumor growth and M2 macrophage polarization by binding to LGALS1 and MIF.
- Published
- 2022
- Full Text
- View/download PDF
47. Multiple responses optimization of antioxidative components extracted from Fenugreek seeds using response surface methodology to identify their chemical compositions
- Author
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Jie Yang, Zhenzhen Zhang, Qimeng Wu, Xiaoyuan Ding, Chenyang Yin, Endong Yang, Dongdong Sun, Weiyun Wang, Yunqiu Yang, and Feng Guo
- Subjects
antioxidant activity ,Fenugreek seeds ,flavonoids ,LC–MS ,multiple response surface optimization ,natural products ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Abstract Fenugreek seeds (Trigonella foenum‐graecum L.), one kind of traditional Chinese medicine, are reported to be of great potential as a new alternative in terms of their bioactive components. In our present study, an ultrasonic‐assisted method was applied in the extraction of antioxidative components from fenugreek seeds. Four factors: ethanol concentration, liquid–solid ratio, sonication time, and sonication power were selected and multiple responses were studied using the response surface methodology (RSM). The effects of factors along with the correlation between all responses (flavonoids content, 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) assay, OH− assay) were studied. The regression model indicated that all four factors are of significant effect on all responses. The model predicted that the ethanol concentration of 72%, solvent‐to‐material ratio of 35 ml/g, ultrasonic time of 41 min, and 500 W of power would provide a flavonoid yield of 9.10 mg/g, DPPH clearance of 80.33%, and OH− clearance of 24.28%, respectively. The confirmation test showed the closeness of the predicted results with those of experimental values. And AB‐8 resin was successfully used to purify the fenuellus hulusi seed extract, and the flavonoid concentration of 78.14% was obtained. Six flavonoids (Swertisin, Puerarin apioside, Jasminoside B, Astragalin, Apigenin‐7‐O‐beta‐D‐glucoside, and Apiin) were successfully identified by the liquid chromatography–mass spectrometry (LC–MS) analysis.
- Published
- 2022
- Full Text
- View/download PDF
48. Observations on breeding behavior of the White-eared Night Heron (Gorsachius magnificus) in northern Guangdong, China
- Author
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Jianyun Gao, Dongdong Su, Yisheng Peng, Zhaolu Wu, Pingsheng Zhong, and Fasheng Zou
- Subjects
education.field_of_study ,Evening ,biology ,Ecology ,Population ,Fledge ,General Engineering ,Zoology ,biology.organism_classification ,Gorsachius magnificus ,Geography ,biology.animal ,Seasonal breeder ,education ,Heron ,Night heron ,Morning - Abstract
The White-eared Night Heron (Gorsachius magnificus) is a Class II protected species in China. It is difficult to observe in the field and its population is small. The present study reports a new distribution site in Nanxiong County, in northern Guangdong Province, where observations of an active nest were made from 4 May to 14 July 2012, the first time consecutive observations have been made for a period of more than two months. The adults flew away from the nest at night, with a peak at 19:00-20:00 and returned in the mornings with a peak at 04:30-05:30. The peak time for feeding young was at 05:00-05:30, accounting for 26.5% of total feeding activities. No feeding occurred from 10:00 to 16:00. The results of our study of its behavior explain why the White-eared Night Heron is usually observed in the evening just before dark, or in the morning before sunrise, at least during the breeding season. There were about 67 days from hatching to fledging, longer than the fledging time of most heron species. This study is the first to cover the entire period of parental care and fills a gap in previous knowledge.
- Published
- 2013
49. Liquid chromatography-tandem mass spectrometry analysis of a ratio-optimized drug pair of Sophora flavescens Aiton and Coptis chinensis Franch and study on the mechanism of anti-colorectal cancer effect of two alkaloids thereof
- Author
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Zihan Chen, Yingying Dong, Qiuying Yan, Qin Li, Chengtao Yu, Yueyang Lai, Jiani Tan, Minmin Fan, Changliang Xu, Liu Li, Weixing Shen, Junfei Gu, Haibo Cheng, and Dongdong Sun
- Subjects
Sophora flavescens Aiton ,Coptis chinensis Franch ,matrine ,berberine ,colorectal cancer ,intestinal microbiota ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
The drug pair consisting of Sophora flavescens Aiton (Sophorae flavescentis radix, Kushen) and Coptis chinensis Franch. (Coptidis rhizoma, Huanglian), as described in Prescriptions for Universal Relief (Pujifang), is widely used to treat laxation. Matrine and berberine are the major active components of Kushen and Huanglian, respectively. These agents have shown remarkable anti-cancer and anti-inflammatory effects. A mouse model of colorectal cancer was used to determine the most effective combination of Kushen and Huanglian against anti-colorectal cancer. The results showed that the combination of Kushen and Huanglian at a 1:1 ratio exerted the best anti-colorectal cancer effect versus other ratios. Moreover, the anti-colorectal cancer effect and potential mechanism underlying the effects of matrine and berberine were evaluated by the analysis of combination treatment or monotherapy. In addition, the chemical constituents of Kushen and Huanglian were identified and quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 67 chemical components were identified from the Kushen–Huanglian drug pair (water extraction), and the levels of matrine and berberine were 129 and 232 µg/g, respectively. Matrine and berberine reduced the growth of colorectal cancer and relieved the pathological conditions in mice. In addition, the combination of matrine and berberine displayed better anti-colorectal cancer efficacy than monotherapy. Moreover, matrine and berberine reduced the relative abundance of Bacteroidota and Campilobacterota at phylum level and that of Helicobacter, Lachnospiraceae_NK4A136_group, Candidatus_Arthromitus, norank_f_Lachnospiraceae, Rikenella, Odoribacter, Streptococcus, norank_f_Ruminococcaceae, and Anaerotruncus at the genus level. Western blotting results demonstrated that treatment with matrine and berberine decreased the protein expressions of c-MYC and RAS, whereas it increased that of sirtuin 3 (Sirt3). The findings indicated that the combination of matrine and berberine was more effective in inhibiting colorectal cancer than monotherapy. This beneficial effect might depend on the improvement of intestinal microbiota structure and regulation of the RAS/MEK/ERK-c-MYC-Sirt3 signaling axis.
- Published
- 2023
- Full Text
- View/download PDF
50. A Simple BODIPY-Based Viscosity Probe for Imaging of Cellular Viscosity in Live Cells
- Author
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Nengyue Gao, Qing-Hua Xu, Young-Tae Chang, Dongdong Su, and Chai Lean Teoh
- Subjects
Boron Compounds ,Fluorescence-lifetime imaging microscopy ,Work (thermodynamics) ,FLIM ,Cell Survival ,Analytical chemistry ,02 engineering and technology ,010402 general chemistry ,sensors ,lcsh:Chemical technology ,01 natural sciences ,Biochemistry ,Article ,Analytical Chemistry ,BODIPY ,fluorescent probes ,viscosity ,cell imaging ,Viscosity ,chemistry.chemical_compound ,Humans ,lcsh:TP1-1185 ,Electrical and Electronic Engineering ,Instrumentation ,Chemistry ,Optical Imaging ,021001 nanoscience & nanotechnology ,Fluorescence ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,Solvent ,Coupling (electronics) ,Fluorescence intensity ,Spectrometry, Fluorescence ,Microscopy, Fluorescence ,Molecular Probes ,Biophysics ,Solvents ,0210 nano-technology ,HeLa Cells - Abstract
Intracellular viscosity is a fundamental physical parameter that indicates the functioning of cells. In this work, we developed a simple boron-dipyrromethene (BODIPY)-based probe, BTV, for cellular mitochondria viscosity imaging by coupling a simple BODIPY rotor with a mitochondria-targeting unit. The BTV exhibited a significant fluorescence intensity enhancement of more than 100-fold as the solvent viscosity increased. Also, the probe showed a direct linear relationship between the fluorescence lifetime and the media viscosity, which makes it possible to trace the change of the medium viscosity. Furthermore, it was demonstrated that BTV could achieve practical applicability in the monitoring of mitochondrial viscosity changes in live cells through fluorescence lifetime imaging microscopy (FLIM).
- Published
- 2016
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