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WOS: 000399725900003, PubMed ID: 29026849, Background: Crimean-Congo Hemorrhagic Fever (CCHF) is a disease characterized by serious course, including acute viral fever, ecchymosis, thrombocytopenia, liver dysfunction and high rate of mortality. Hypoxia Inducible Factor-1 alpha (HIF-1 alpha) and Vascular Endothelial Growth Factor-A (VEGF-A) play an important role both in the inflammatory process and plasma leakage. The aim of this study was to define HIF-1 alpha and VEGF-A serum levels obtained from CCHF patients and control group and to investigate whether these factors were correlated with the pathogenesis of this disease. Methods: Thirty cases younger than 17yr confirmed by RT-PCR and/or ELISA for CCHF were included in this study. Thirty age and sex matched healthy peoples were enrolled as controls. Blood samples collected from the patient and control groups. Serum levels of HIF-1 alpha and VEGF-A were measured with ELISA. Results: Levels of HIF-1 alpha and VEGF-A were statistically significantly increased in CCHF patients compared to the control group (P < 0.05). A significant positive correlation was found between the levels of HIF-1 alpha and VEGF-A in the patient group (P < 0.01). The levels of ALT, AST, CK, aPTT, WBC and Thrombocyte count were significantly higher in the patients than in the control group (P < 0.001). A positive correlation was found among the levels of AST and CK from biochemical parameters and VEGF and HIF-1 alpha in the patient group (P < 0.05) Conclusion: HIF-1 alpha and VEGF-A might play an important role in CCHF pathogenesis.

WOS: 000461427300003, Objective: SCUBE1 [signal peptide-CUB (complement C1r/C1 s)-EGF (epidermal growth factor)-like domain-containing protein 1] is a novel biochemical marker. SCUBE1 is thought to play roles both in platelet activation and inflammation, which are important stages for the development of acute ischemic stroke (AIS). The purpose of our study was to determine the diagnostic and prognostic values and temporal change of plasma SCUBE1 levels in AIS patients. Materials and methods: Thirty-five patients diagnosed with AIS at the Cumhuriyet University Faculty of Medicine Neurology Department, between June and December 2017, and a control group of 35 healthy volunteers were included. Results: Median first day SCUBE1 value in the patient group was 97.51 ng/mL, and the median 7th day SCUBE1 value was 32.72 ng/mL. Median control group SCUBE1 value was 27.51 ng/mL. The first day SCUBE1 levels were significantly higher than the 7th day and the control group SCUBE1 levels (p = 0.001, p < 0.001, respectively). The ROC analysis showed that SCUBE1 levels above 68.8 ng/mL can be used as an indicator with high sensitivity and specificity for AIS diagnosis. Multivariate analysis revealed that the first day SCUBE1 had significant independent effects on development of AIS. In correlation analysis, plasma SCUBE1 levels showed a significantly positive correlation with lesion volume, NIHSS and MRS values (p < 0.01). Conclusion: First day plasma SCUBE1 values in AIS patients rised at significant levels compared to the control group. SCUBE1 could use both in the early diagnosis and prediction of prognosis and lesion volume of AIS patients.

WOS: 000373106100002, This study aims to investigate the relationship between sulfurtransferase (STS) activities [rhodanese (TST), mercaptopyruvate sulfurtransferase (MST)] involved in the catalysis of several biochemical reactions including detoxification of cyanide (CN-), restructuring of Fe-S cluster in proteins, and detoxification of oxygen radicals. Rats with type 1 diabetes mellitus induced by streptozotocin (STZ) were anesthetized at 14th day, and liver, lung, kidney, and heart tissues were extracted. All samples were homogenized, and mitochondrial parts were separated. Same processes were performed also in the control group, and TST and MST activities were measured in each part. The homogenate MST (MST (Homo) .) activities of the type 1 diabetes mellitus group were compared with the control group, and a decrease was observed in the lung, liver, and kidney, respectively; at the same time, an increase was seen in the heart tissue. The mitochondrial MST (MST (Mito) .) activities of rats with type 1 diabetes mellitus group were compared with the control group, and a decrease was found in all tissues. The highest decrease in the TST (Mito) . level of rats with type 1 diabetes mellitus was observed in kidney tissue. The TST activities of the type 1 diabetes mellitus group were compared with the control group, and a decrease was observed in the liver, lung, and kidney, respectively; at the same time, an increase was seen in the heart tissue. It is demonstrated in the present study that decreases occur both in enzyme levels of tissue homogenates and in mitochondria, of rats with induced type 1 diabetes mellitus. However, these results were not statistically significant. In the presence of these findings, we think that kidney, liver, lung, and heart tissue can be affected by type 1 diabetes in the long term.

WOS: 000337256000005, PubMed: 24337212, Aim: Oxidative stress, which is widely recognised as an important feature of many diseases, can be defined as an increased formation of reactive oxygen species or decreased antioxidant defense. In this study we measured plasma vitamin E levels and total antioxidant activity (AOA) in patients with slow coronary flow (SCF). Methods: The plasma vitamin E levels and AOA were measured in 40 patients with angiographically diagnosed SCF. Forty subjects with normal coronary flow (NCF) served as the control group. SCF and NCF were analysed, and blood samples were taken for plasma vitamin E levels and AOA. Plasma vitamin E levels and AOA in patients with SCF were evaluated and compared to those of patients with NCF. Results: There was no significant difference between the two groups in terms of plasma AOA, lipid profile and C-reactive protein (CRP) levels but there was a significant difference in vitamin E levels between the two groups (p = 0.001). Conclusion: Vitamin E levels were found to be lowered in patients with SCF compared to the NCF group. The association between smoking and vitamin E levels is worth further investigating in larger samples.

WOS: 000302676600001, PubMed ID: 22536214, Objective. Oxidative stress is considered to be the main factor in the development of diabetic complications and tissue injury. our objective was to investigate and compare the oxidant/antioxidant conditions and detoxification mechanisms of the liver, lung, kidney, cardiac tissues, and mitochondria of rats with diabetes induced by streptozocin (STZ). Methods. Rats with diabetes induced by streptozocin were anesthetized by administering 90 mg/kg ketamine hydrochloride and 3mg/kg xylazine hydrochloride. Thoracic cavities were incised open; liver, lung, kidney, and cardiac tissues were removed and stored at -70 degrees C. All samples were homogenized and mitochondrial fractions were separated. Total Antioxidant Status (TAS), Total Oxidant Status (TOS), Oxidative Stress Index (OSI), Paraoxonase (PON), Arylesterase, Catalase (Cat), Malondialdehyde (MDA), and Glutathion-S-transferase were measured in each fraction. Results. MDA and TOS levels were significantly increased in liver tissues, and TOS and OSI were increased in the mitochondrial fractions of diabetic rats. These increases were not statistically significant compared to the control group. No significant differences were determined in the antioxidant and GST activities. Conclusion. According to our results, oxidative stress has not developed in rats with diabetes induced by streptozocin. The detoxification system was induced; however, this induction did not differ significantly from the controls.

WOS: 000354637200021, Serum levels of amylase and lipase are the most widely used biochemical indicators of pancreatic inflammation. They are the basic markers used when making a diagnosis of pancreatitis. However, they are not necessarily the gold standard since the specificity and sensitivity of high amylase and lipase levels are low. The purpose of this study was to show the predictive value of C/EBP homologous protein in diagnosing acute pancreatitis. Thirty-two cases of acute pancreatitis that were admitted to the Department of Emergency Medicine of the Bezmialem Vakif University between November 2013 and July 2014 were analyzed. The diagnosis of acute pancreatitis was made based on characteristic signs and symptoms, increased serum enzyme levels, and abnormal findings on diagnostic imaging. When compared with the control group, the serum C/EBP homologous protein levels were found to be significantly increased with routine diagnostic methods in cases of acute pancreatitis. During the process of pancreatic inflammation, the level of C/EBP homologous protein increased independently in the etiopathogenesis of acute pancreatitis., Scientific Research Projects Committee of Bezmialem Vakif University, Istanbul, Turkey, This study was financially supported by the Scientific Research Projects Committee of Bezmialem Vakif University, Istanbul, Turkey

WOS: 000331598600002, Changes in the fatty acid (FA) composition of unfed and fed rainbow trout (Oncorhynchus mykiss) larvae were determined by gas chromatography. Total lipids were extracted from samples by homogenization in chloroform/methanol (2:1, v/v). Fatty acid methyl esters were prepared from total lipid. The sample was saponified with methanolic potassium hydroxide (KOH). The fatty acids esterified with boron trifluoride-methanol (BF3-methanol) and fatty acid methyl esters were analyzed with a Shimadzu GCMSQP5000 gas chromatograph.In starved larvae, there was an apparent preference in utilization of oleic acid (C18:1 omega-9) in monounsaturated fatty acids than in the fed larvae. In both starved and fed larvae, palmitoleic (C16:1 omega-7) acid was preferentially kept during the same period. Larvae kept 29 days under starvation conditions exhausted linolenic acid (C18:3 omega-6), eicosanoic acid (C20:0), docosanoic acid (C22:0) and docosatrienoic acid (C22:3 omega-3). They utilized less eicosapentaenoic (C20:5 omega-3; EPA) acid and conserved strongly docosahexaenoic (C22:6 omega-3; DHA) acid., Cumhuriyet University (Sivas, Turkey), We thanks the Research Fund of Cumhuriyet University (Sivas, Turkey) for supporting this research and the local and commercial Fish Farm in Sivas for providing the eggs and sperm samples.