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Comparison of Oxidant/Antioxidant, Detoxification Systems in Various Tissue Homogenates and Mitochondria of Rats with Diabetes Induced by Streptozocin
- Source :
- Experimental Diabetes Research, Experimental Diabetes Research, Vol 2012 (2012)
- Publication Year :
- 2012
- Publisher :
- Hindawi Limited, 2012.
-
Abstract
- WOS: 000302676600001<br />PubMed ID: 22536214<br />Objective. Oxidative stress is considered to be the main factor in the development of diabetic complications and tissue injury. our objective was to investigate and compare the oxidant/antioxidant conditions and detoxification mechanisms of the liver, lung, kidney, cardiac tissues, and mitochondria of rats with diabetes induced by streptozocin (STZ). Methods. Rats with diabetes induced by streptozocin were anesthetized by administering 90 mg/kg ketamine hydrochloride and 3mg/kg xylazine hydrochloride. Thoracic cavities were incised open; liver, lung, kidney, and cardiac tissues were removed and stored at -70 degrees C. All samples were homogenized and mitochondrial fractions were separated. Total Antioxidant Status (TAS), Total Oxidant Status (TOS), Oxidative Stress Index (OSI), Paraoxonase (PON), Arylesterase, Catalase (Cat), Malondialdehyde (MDA), and Glutathion-S-transferase were measured in each fraction. Results. MDA and TOS levels were significantly increased in liver tissues, and TOS and OSI were increased in the mitochondrial fractions of diabetic rats. These increases were not statistically significant compared to the control group. No significant differences were determined in the antioxidant and GST activities. Conclusion. According to our results, oxidative stress has not developed in rats with diabetes induced by streptozocin. The detoxification system was induced; however, this induction did not differ significantly from the controls.
- Subjects :
- Male
lcsh:Internal medicine
medicine.medical_specialty
lcsh:Specialties of internal medicine
Article Subject
Endocrinology, Diabetes and Metabolism
lcsh:Medicine
Kidney
medicine.disease_cause
lcsh:Diseases of the endocrine glands. Clinical endocrinology
Antioxidants
Diabetes Mellitus, Experimental
Arylesterase
Lipid peroxidation
chemistry.chemical_compound
lcsh:RC581-951
Malondialdehyde
Internal medicine
Diabetes mellitus
medicine
Animals
Rats, Wistar
lcsh:RC31-1245
Lung
lcsh:RC648-665
biology
Myocardium
lcsh:R
Paraoxonase
Ketamine hydrochloride
General Medicine
Catalase
medicine.disease
Glutathione
Mitochondria
Rats
Streptozocin
Oxidative Stress
Endocrinology
Liver
chemistry
biology.protein
Female
Lipid Peroxidation
Oxidative stress
Research Article
Subjects
Details
- ISSN :
- 16875303 and 16875214
- Volume :
- 2012
- Database :
- OpenAIRE
- Journal :
- Experimental Diabetes Research
- Accession number :
- edsair.doi.dedup.....3e14b1f8e3c0734be7e7d48307e4aab2