24 results on '"Villanueva, Marvin"'
Search Results
2. Evaluating the Usability, Perceived Performance, and Perceived Effects of KBGAN iHealth© and KBGAN iFeed© Mobile Apps for Buffalo Management in Selected Municipalities in the Philippines
- Author
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Palacpac, Eric Parala, Pacsa Balingit, Kae Ann Marie, Dinulos Bonifacio, Airon Andrew, Villanueva, Marvin Ardeza, Tolentino, Randolph Bautista, Uy-DeGuia, Mary Rose De Leon, Tolentino Llantada, Phoebe Lyndia, Castillo, Charity Ignacio, Brul, Beverly Janabajal, Adecer Rubio, Hannah Carmela, Abes, Maica Miclat, Palacpac, Eric Parala, Pacsa Balingit, Kae Ann Marie, Dinulos Bonifacio, Airon Andrew, Villanueva, Marvin Ardeza, Tolentino, Randolph Bautista, Uy-DeGuia, Mary Rose De Leon, Tolentino Llantada, Phoebe Lyndia, Castillo, Charity Ignacio, Brul, Beverly Janabajal, Adecer Rubio, Hannah Carmela, and Abes, Maica Miclat
- Abstract
This study evaluates the KBGAN iHealth© and KBGAN iFeed© mobile apps designed for buffalo health and feeding management, particularly for agricultural extension professionals (AEPs) in selected Philippine municipalities. These apps aim to address challenges in buffalo management, such as limited access to veterinary expertise, personalized recommendations, organized data, communication channels, and difficulties in calculating ideal feed compositions and meeting the distinct needs of smallholder farmers and AEPs. Despite System Usability Scale (SUS) scores indicating marginal acceptability for both apps, weighted mean scores by AEPs for statements assessed on a 5-point Likert scale (1 as strongly disagree and 5 as strongly agree), demonstrate that AEPs reported high confidence in the accuracy of buffalo health diagnostics (Mean of 4.20) and health management recommendations (Mean of 4.17) provided by KBGAN iHealth©. Similarly, KBGAN iFeed© received favorable ratings, with AEPs expressing agreement on the accuracy of feeding recommendations (Mean of 3.89) and the facilitation of feeding ration computations (Mean of 4.00). These positive perceived performance outcomes, coupled with increased confidence (Mean of 4.03) and motivation (Mean of 4.00) among AEPs, suggest the potential for frequent and consistent app usage despite usability concerns. Chi-square tests examining the relationship between AEP characteristics and SUS scores for the apps reveal significant associations between AEPs' education and experience levels and SUS scores for KBGAN iFeed©. The choice of IT device also influences KBGAN iHealth© SUS scores. Proposed enhancements by AEPs include refining algorithms, improving the user interface for navigation, speed, and efficiency, and incorporating features such as photo uploads and geotagging.
- Published
- 2024
3. Sensitive detection of Mycobacterium bovis in spiked milk using polymerase chain reaction assay
- Author
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Villanueva, Marvin A, primary, Suzuki, Yasuhiko, additional, Nakajima, Chie, additional, Suzuki, Haruka, additional, Fukushima, Yukari, additional, and Mingala, Claro Niegos, additional
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- 2023
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4. Utilization of Plant Material Extracts as Natural Acid-Base Indicators: An Example of At-Home Lab Experiment in the New Normal Learning Set-Up
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Reyes, Darwin, Santos, Miguel R., Cruz, Jocelyn B., Ascaño, Faye G., Baldovino, Beverly B., Camarillo, Bernadette L., Recheline V. Inoncillo, Saavedra, Lester Jayson L., Czarabelle Dauz, Ducut, Alvin R., Guela, Mery Grace B., Lanuza, Elena C., Romnick B. Nabor, Policarpio, John Ronald T., Dominador M. Santos, Jr., Keneth S. Tolentino, Villanueva, Marvin D., and Sharrise A. Waje
- Subjects
acid-base chemistry ,natural indicators ,lab at-home ,Remote Teaching and Learning ,plant materials - Abstract
In response to the remote learning and teaching setup in science due to the COVID-19 pandemic, a lab at-home experiment was conducted where various plant materials were prepared and used as natural acid-base indicators for various household substances. The experiment is designed for the students to apply the fundamentals of intensive laboratory for science education: laboratory safety, laboratory apparatus, laboratory techniques, data documentation, and lab report writing. Presented herein are the results obtained during the experiments conducted at home that focused on the application of various plant extracts as natural indicator. Observable color changes showed that the tested plant extracts responded to the acidity and alkalinity of the various household substances. The science behind the color changes is attributed to the presence of various phytochemicals that change colors when protonated and deprotonated. The potential of the results obtained in the experiments is very promising as it can be further developed and optimized to an analytical procedure that can be applied in testing of acidity and alkalinity in various applications such as food safety and quality, environmental monitoring, materials testing, pharmaceutical analysis, and clinical diagnostics. Moreover, the experiment explored here presented lab teaching strategies that can be applied in the remote learning setup: formulate and design a home-based laboratory experiment that will cover the underlying scientific theories and skills and provide the students the opportunity to explore, improvise, apply, and present information that will holistically develop them as science educators for their current and prospective students., CITE AS: Reyes, D., Santos, M., Cruz, J., Ascaño, F., Baldovino, B., Camarillo, B., ... & Waje, S. (2022). Utilization of Plant Material Extracts as Natural Acid-Base Indicators: An Example of At-Home Lab Experiment in the New Normal Learning Set-Up. The Quest: Journal of Multidisciplinary Research and Development, 1(2)., {"references":["1.\tAdusei, E. B. A., Adosraku, R. K., Oppong-Kyekyeku, J., Amengor, C. D. K. (2019). Investigation of acid-base indicator property of plumbagin from Plumbago zeylanica Linn, International Journal of Analytical Chemistry, 2019, Article ID 4061927, 13 pages. https://doi.org/10.1155/2019/4061927","2.\tBabinčáková, M., & Bernard, P. (2020). Online experimentation during COVID-19 secondary school closures: Teaching methods and student perceptions. Journal of Chemical Education, 97(9), 3295–3300. https://doi.org/10.1021/acs.jchemed.0c00748","3.\tBernardo M. K. O. & Organo V. G. (2014). Chlorophyll as a simple, inexpensive and environment-friendly colorimetric indicator for NO2 gas. Oriental Journal of Chemistry, 30(2), 445-449. http://dx.doi.org/10.13005/ojc/300206","4.\tCaruana, D.J., Salzmann, C.G. & Sella, A. (2020). Practical science at home in a pandemic world. Nature Chemistry. 12, 780–783. https://doi.org/10.1038/s41557-020-0543-z","5.\tDiaconeasa, Z., Știrbu, I., Xiao, J., Leopold, N., Ayvaz, Z., Danciu, C., Ayvaz, H., Stǎnilǎ, A., Nistor, M., & Socaciu, C. (2020). Anthocyanins, vibrant color pigments, and their role in skin cancer prevention. Biomedicines, 8(9), 336. https://doi.org/10.3390/biomedicines8090336","6.\tGalingana M. O & Organo V. G. (2016). A simple colorimetric procedure for differentiating anions using flower pigments from Anthurium andreanum. Oriental Journal of Chemistry, 32(3), 1347-1352. http://dx.doi.org/10.13005/ojc/320308","7.\tGuidote, A. J. M. (2020). Teaching college chemistry in the time of COVID-19 pandemic: A personal account of teaching in the old normal vs. the new normal. KIMIKA, 31(1), 70-75. https://doi.org/10.26534/kimika.v31i1.70-75","8.\tHazra, A. (2021). Indicator made from countryside flower petals. Oriental Journal Of Chemistry, 37. 1252-1256. 10.13005/ojc/370534","9.\tIbarra-Rivera, T., Delgado-Montemayor, C., Oviedo-Garza, F., Pérez-Meseguer, J., Rivas-Galindo, V., Waksman-Minsky, N. & Pérez-López, L. (2020). Setting up an educational column chromatography experiment from home. Journal of Chemical Education, 97(9), 3055-3059. https://doi.org/10.1021/acs.jchemed.0c00532","10.\tKapilraj, N., Keerthanan S., & Sithambaresan, M. (2019). Natural plant extracts as acid-base indicator and determination of their pKa value. Journal of Chemistry, 2019, Article ID 2031342, 6 pages. https://doi.org/10.1155/2019/2031342","11.\tLavanya, D., Guna, G., Purushothom, A., & Pallavi, A. (2018). Green Chemistry: A study on acid-base indicator property of various flower pigments. International Journal of Research and Development in Pharmacy & Life Sciences, 7. 3155-3163. 10.21276/IJRDPL.2278-0238.2018.7(6).3155-3163","12.\tNguyen, J. G., & Keuseman, K. J. (2020). Chemistry in the kitchen laboratories at home. Journal of Chemical Education, 97(9), 3042–3047. https://doi.org/10.1021/acs.jchemed.0c00626","13.\tParistiowati, M., Moersilah, M & Stephanie, M. M., Zulmanelis, Z. Idroes, R., & Puspita, R. A. (2019). Rosa sp and Hibiscus sabdariffa L extract in ethanol fraction as acid base indicator: Application of green chemistry in education. Journal of Physics: Conference Series, 1402. 055041. 10.1088/1742-6596/1402/5/055041","14.\tPatil, N. & Datar, A. (2015). Extraction, stability and separation of anthocyanins of Ixora coccinea Linn. International Journal of Pharmacy and Pharmaceutical Sciences, 7, 198-202.","15.\tSampim, T., Phupa, S. & Sampim, S. (2019). Efficiency and effectiveness of universal indicator from native plants in south of Thailand. Journal of Physics: Conferences Series, 1340, 012018. 10.1088/1742-6596/1340/1/012018","16.\tSanchez, J. M., Fernandez, M. J., Abgao, J. M., Sarona, H., Asenjo, S. B., Guiroy, B., Oponda, A. J., & Vale, X. (2021). Experimenting on natural acid-base indicators: a home-based chemistry activity during the COVID-19 pandemic as evaluated by teachers. KIMIKA, 32(1), 34-45. https://doi.org/10.26534/kimika.v32i1.34-45","17.\tSanjay, P., Isaivani, I., K., Deepa, K., Madhavan, J., & Senthil, S. (2019). The preparation of dye sensitized solar cells using natural dyes extracted from Phytolacca icosandra and Phyllanthus reticulatus with ZnO as Photoanode. Materials Letters, 244. 10.1016/j.matlet.2019.02.072.","18.\tSenathirajah, T., Rasalingam, S. & Ganeshalingam, S. (2017). Extraction of the cyanidin-3-sophoroside from Hibiscus rosa-sinensis: an efficient natural indicator over a wide range of acid-base titrations. Journal of Natural Product and Plant Resources, 7(3), 1–7.","19.\tStephanie, M. M., Moersilah M., & Paristiowati, M. (2020). Jambolan fruit peels (Syzygium cumini l. skeels) as substitute for synthetic acid base indicators: implementation of the ESD concept. Journal of Physics: Conference Series, 1521. 042073. 10.1088/1742-6596/1521/4/042073","20.\tVijayanand, S. & Khalid, M. (2019). Study of Brassica oleracea as natural alternative to synthetic indicator. Asian Journal of Chemistry, 31. 251-254. 10.14233/ajchem.2019.21486.","21.\tVilla-Rivera, M. G., & Ochoa-Alejo, N. (2020). Chili pepper carotenoids: nutraceutical properties and mechanisms of action. Molecules (Basel, Switzerland), 25(23), 5573. https://doi.org/10.3390/molecules25235573","22.\tZheng, L., Liu, L., Yu, J., & Shao, P. (2022). Novel trends and applications of natural pH-responsive indicator film in food packaging for improved quality monitoring. Food Control, 134(108769). https://doi.org/10.1016/j.foodcont.2021.108769"]}
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- 2023
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5. Prevalence and distribution of blaCTX-M, blaSHV, blaTEM genes in extended- spectrum β- lactamase- producing E. coli isolates from broiler farms in the Philippines
- Author
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Gundran, Romeo S., Cardenio, Paul A., Villanueva, Marvin A., Sison, Fredelon B., Benigno, Carolyn C., Kreausukon, Kwanchai, Pichpol, Duangporn, and Punyapornwithaya, Veerasak
- Published
- 2019
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6. Sensitive detection of Mycobacterium bovis in spiked milk using a polymerase chain reaction assay.
- Author
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Villanueva, Marvin A., Yasuhiko Suzuki, Chie Nakajima, Haruka Suzuki, Yukari Fukushima, and Mingala, Claro N.
- Subjects
- *
MYCOBACTERIUM bovis , *POLYMERASE chain reaction , *NUCLEIC acid isolation methods , *RAW milk , *TUBERCULOSIS in cattle , *PUBLIC health - Abstract
Bovine tuberculosis is a chronic zoonotic disease that affects both animal and human health and imposes serious public health concerns in the world. Intake of non-pasteurized milk is considered the most probable vehicle for the transmission of pathogenic bacteria. In this study, the detection of Mycobacterium bovis BCG in spiked milk using a polymerase chain reaction was performed. The performance of two DNA extraction methods, CTAB/phenol:chloroform:isoamyl alcohol and EXTRAGENMB were also evaluated. In addition, Mycobacterial concentration was tried to determine using the Standard/Viable Plate Count Method and Spectrophotometric (Turbidimetric) Method. PCR successfully detected M. bovis BCG in spiked milk, detecting approximately up to two bacilli per reaction. The two DNA extraction methods were effective in the isolation of amplifiable DNA, having the advantage of EXTRAGENMB in terms of (1) shorter duration of DNA extraction, (2) less sample manipulation, and (3) ease of execution of the procedure. Quantitative determination of the Mycobacterial population, however, failed to quantify the bacterial concentration per dilution, suggesting that CFU concentration should be considered an approximation. It is expected that this method can be used for the detection of M. bovis in raw milk samples. [ABSTRACT FROM AUTHOR]
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- 2023
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7. Prevalence and Characterization of Quinolone-Resistance Determinants in Escherichia coli Isolated from Food-Producing Animals and Animal-Derived Food in the Philippines
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Belotindos, Lawrence, Villanueva, Marvin, Miguel, Joel, Bwalya, Precious, 1000070516723, Harada, Tetsuya, 1000010332454, Kawahara, Ryuji, 1000060435964, Nakajima, Chie, Mingala, Claro, 1000090206540, Suzuki, Yasuhiko, Belotindos, Lawrence, Villanueva, Marvin, Miguel, Joel, Bwalya, Precious, 1000070516723, Harada, Tetsuya, 1000010332454, Kawahara, Ryuji, 1000060435964, Nakajima, Chie, Mingala, Claro, 1000090206540, and Suzuki, Yasuhiko
- Abstract
Antimicrobial resistance to quinolones, which constitutes a threat to public health, has been increasing worldwide. In this study, we investigated the prevalence of quinolone-resistant determinants in Escherichia coli not susceptible to quinolones and isolated from food-producing animals and food derived from them, in the Philippines. A total of 791 E. coli strains were isolated in 56.4% of 601 beef, chicken, pork, egg, and milk samples, as well as environmental, cloacal, and rectal swab-collected samples from supermarkets, open markets, abattoirs, and poultry, swine, and buffalo farms. Using the disc diffusion method, it was determined that 78.6% and 55.4% of the isolates were resistant to at least one antimicrobial and multiple drugs, respectively. In 141 isolates not susceptible to quinolones, 115 (81.6%) harbored quinolone-resistant determinants and had mutations predominantly in the quinolone-resistance determining regions (QRDRs) of gyrA and parC. Plasmid-mediated, quinolone resistance (PMQR) and Qnr family (qnrA1, qnrB4, and qnrS1) genes were detected in all isolates. Forty-eight sequence types were identified in isolates harboring mutations in QRDR and/or PMQR genes by multilocus sequence typing analysis. Moreover, 26 isolates harboring mutations in QRDR and/or PMQR genes belonged mostly to phylogroup B1 and Enteroaggregative E. coli. In conclusion, a high prevalence of E. coli was found in food-producing animals and products derived from them, which could potentially spread high-risk clones harboring quinolone-resistance determinants.
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- 2021
8. Prevalence and Characterization of Quinolone-Resistance Determinants in Escherichia coli Isolated from Food-Producing Animals and Animal-Derived Food in the Philippines
- Author
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Belotindos, Lawrence, primary, Villanueva, Marvin, additional, Miguel, Joel, additional, Bwalya, Precious, additional, Harada, Tetsuya, additional, Kawahara, Ryuji, additional, Nakajima, Chie, additional, Mingala, Claro, additional, and Suzuki, Yasuhiko, additional
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- 2021
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9. Characterisation of plasmids harbouring qnrA1, qnrS1, and qnrB4in E. coliisolated in the Philippines from food-producing animals and their products
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Belotindos, Lawrence P., Tsunoda, Risa, Villanueva, Marvin A., Nakajima, Chie, Mingala, Claro N., and Suzuki, Yasuhiko
- Abstract
Determinants showing plasmid-mediated quinolone resistance, which usually leads to antimicrobial ineffectiveness, have become an emerging clinical problem. In our previous study in the Philippines, a high prevalence of qnrdeterminants was found in clinical samples and food-producing animals and their food products. However, no qnr-carrying plasmids have been investigated in animals or animal-derived foods. Hence, in the present, we aimed to characterise qnr-carrying plasmids in Escherichia coliisolated from the food supply chain.
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- 2022
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10. Molecular epidemiology of pathogenic Leptospira spp. among large ruminants in the Philippines
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VILLANUEVA, Marvin A., MINGALA, Claro N., BALBIN, Michelle M., NAKAJIMA, Chie, ISODA, Norikazu, SUZUKI, Yasuhiko, and KOIZUMI, Nobuo
- Subjects
Leptospira ,Molecular Epidemiology ,Full Paper ,Buffaloes ,animal diseases ,Philippines ,Cattle Diseases ,Bacteriology ,the Philippines ,Sequence Analysis, DNA ,bacterial infections and mycoses ,flaB ,cattle ,Prevalence ,Animals ,water buffalo ,Leptospirosis - Abstract
The extent of Leptospira infection in large ruminants resulting to economic problems in livestock industry in a leptospirosis-endemic country like the Philippines has not been extensively explored. Therefore, we determined the prevalence and carrier status of leptospirosis in large ruminants using molecular techniques and assessed the risk factors of acquiring leptospirosis in these animals. Water buffalo and cattle urine samples (n=831) collected from 21 farms during 2013-2015 were subjected to flaB-nested PCR to detect pathogenic Leptospira spp. Leptospiral flaB was detected in both species with a detection rate of 16.1%. Leptospiral DNA was detected only in samples from animals managed in communal farms. Sequence analysis of Leptospira flaB in large ruminants revealed the formation of three major clusters with L. borgpetersenii or L. kirschneri. One farm contained Leptospira flaB sequences from all clusters identified in this study, suggesting this farm was the main source of leptospires for other farms. This study suggested that these large ruminants are infected with various pathogenic Leptospira species causing possible major economic loss in the livestock industry as well as potential Leptospira reservoirs that can transmit infection to humans and other animals in the Philippines.
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- 2016
11. Serological investigation of Leptospira infection and its circulation in one intensive-type water buffalo farm in the Philippines
- Author
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Villanueva, Marvin A., Mingala, Claro N., Gloriani, Nina G., Yanagihara, Yasutake, Isoda, Norikazu, Nakajima, Chie, Suzuki, Yasuhiko, and Koizumi, Nobuo
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Leptospira ,intensive-type farming ,leptospirosis ,water buffalo ,the Philippines - Published
- 2016
12. Emerging infectious Diseases in Water Buffalo : An Economic and Public Health Concern
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Villanueva, Marvin A.
- Subjects
Medical / Veterinary Medicine - Abstract
Water buffalo is an indispensable livestock in Asia and other countries due to its high meat and milk quality, aside from draft power source. It adapts well to tropical climate and has significant contribution to the livestock industry, provided with improved breeding and good animal husbandry practices. Infectious diseases are hindrance to good reproductive performance of livestock, resulting in huge economic loss. In addition, most of these diseases are zoonotic, posing serious threats on human health. However, its degree of severity varies in each region and is often overlooked. This chapter reviews the common and current updates on emerging bacterial, viral, protozoal, fungal and endoparasitic pathogens that infect water buffaloes worldwide. All of the diseases directly affect the animals’ health condition except for schistosomiasis where water buffalo played an important role as shedder of infection to humans. Leptospirosis, brucellosis, Bovine Tb, BVDV and fasciolosis have projected economic impact to water buffalo industry as well as its effect as zoonoses. However, the data seem underquantified since most are neglected diseases and are highly prevalent in developing countries. Further studies are needed particularly in countries where water buffalo is the major livestock than cattle to fully utilize the potential of the animal.
- Published
- 2018
13. Trypanosoma evansi AND Neospora caninum AMONG WATER BUFFALOES (Bubalus bubalis) IN THE PHILIPPINES.
- Author
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Niegos Mingala, Claro, Venturina, Virginia M., Rivera, Christopher V., Balbin, Michelle M., Villanueva, Marvin A., and Abenoja, Jaypee A.
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NEOSPORA caninum ,WATER buffalo ,TRYPANOSOMA ,ENZYME-linked immunosorbent assay ,SERUM ,POLYMERASE chain reaction - Abstract
The study determined the positivity rate of Trypanosoma evansi and Neospora caninum antibodies in water buffaloes in the province of Nueva Ecija, Philippines using Polymerase Chain Reaction (PCR) for T. evansi and competitive Enzyme-linked Immunosorbent Assay (cELISA) for N. caninum antibodies. A total of 100 whole blood and 100 serum samples were collected to test for T. evansi and N. caninum, respectively. Rotat 1.2 VSG gene was target using PCR for T. evansi detection. Neospora caninum antibody detection was done from the serum samples using cELISA test kit. Results revealed that the positivity rate of T. evansi in Nueva Ecija was 11% (11/100). The positive animals identified were from the municipalities of Muñoz (4/16; 25%), Sta. Rosa (3/13; 23.08%) and Talugtug (4/16; 25%). The seropositive rate of Nueva Ecija for N. caninum. was 46% (46/100), seropositive animals were identified in Cabanatuan City, 57.14% (4/7); Science City of Muñoz, 43.14% (22/51); Sta. Rosa, 40% (4/10); Sto. Domingo, 50% (6/12); and Talugtug 50% (10/20). The seropositivity rate of N. caninum and the presence of T. evansi in Nueva Ecija may contribute to the cases of abortions in the province and further studies should be employed to confirm the association of these organisms to abortion cases on water buffaloes.. [ABSTRACT FROM AUTHOR]
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- 2020
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14. Evaluation of LAMP for detection and/or screening of Leptospira spp. infection among domestic animals in the Philippines
- Author
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Tubalinal, Gabriel, primary, Balbin, Michelle, additional, Villanueva, Marvin, additional, Domingo, Clarissa, additional, and Mingala, Claro, additional
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- 2018
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15. フィリピンの水牛(Bubalis bubalis)が保有する病原性レプトスピラの疫学的研究
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Villanueva, Marvin Ardeza, 鈴木, 定彦, 東, 秀明, 磯田, 典和, and 中島, 千絵
- Abstract
(主査) 教授 鈴木 定彦, 教授 東 秀明, 准教授 磯田 典和, 准教授 中島 千絵, 獣医学研究科(獣医学専攻)
- Published
- 2016
16. フィリピンの水牛(Bubalis bubalis)が保有する病原性レプトスピラの疫学的研究 [論文内容及び審査の要旨]
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Villanueva, Marvin Ardeza, 鈴木, 定彦, 東, 秀明, 磯田, 典和, and 中島, 千絵
- Abstract
(主査) 教授 鈴木 定彦, 教授 東 秀明, 准教授 磯田 典和, 准教授 中島 千絵, 獣医学研究科(獣医学専攻)
- Published
- 2016
17. Characterization of drug resistance-associated TevAT1 gene of Trypanosoma evansi from Philippine water buffaloes (Bubalus bubalis).
- Author
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Mingala, Claro N., Pasag, Alma Corazon P., Salinas, Marvin Bryan S., Balbin, Michelle M., and Villanueva, Marvin A.
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- 2019
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18. Development and evaluation of an interferon-γ release assay in Asian elephants (Elephas maximus)
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PAUDEL, Sarad, primary, VILLANUEVA, Marvin A., additional, MIKOTA, Susan K., additional, NAKAJIMA, Chie, additional, GAIRHE, Kamal P., additional, SUBEDI, Suraj, additional, RAYAMAJHI, Nabin, additional, SASHIKA, Mariko, additional, SHIMOZURU, Michito, additional, MATSUBA, Takashi, additional, SUZUKI, Yasuhiko, additional, and TSUBOTA, Toshio, additional
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- 2016
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19. A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in Urine
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Koizumi, Nobuo, 1000060435964, Nakajima, Chie, Harunari, Tsunehito, Tanikawa, Tsutomu, Tokiwa, Toshihiro, Uchimura, Eriko, Furuya, Tokujiro, Mingala, Claro Niegos, Villanueva, Marvin Ardeza, Ohnishi, Makoto, 1000090206540, Suzuki, Yasuhiko, Koizumi, Nobuo, 1000060435964, Nakajima, Chie, Harunari, Tsunehito, Tanikawa, Tsutomu, Tokiwa, Toshihiro, Uchimura, Eriko, Furuya, Tokujiro, Mingala, Claro Niegos, Villanueva, Marvin Ardeza, Ohnishi, Makoto, 1000090206540, and Suzuki, Yasuhiko
- Abstract
We developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs, a 16S rRNA gene, of pathogenic Leptospira spp. in urine. The method enables detection of two leptospiral cells per reaction following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB-nested PCR.
- Published
- 2012
20. Development and evaluation of an interferon-γ release assay in Asian elephants (Elephas maximus).
- Author
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Sarad PAUDEL, VILLANUEVA, Marvin A., MIKOTA, Susan K., Chie NAKAJIMA, GAIRHE, Kamal P., SUBEDI, Suraj, RAYAMAJHI, Nabin, Mariko SASHIKA, Michito SHIMOZURU, Takashi MATSUBA, Yasuhiko SUZUKI, and Toshio TSUBOTA
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ELEPHANT diseases ,IMMUNOLOGICAL blood tests ,INTERFERON gamma release tests - Abstract
We developed an interferon-γ release assay (IGRA) specific for Asian elephants (Elephas maximus). Whole blood collected from forty captive Asian elephants was stimulated with three different mitogens i.e., phytohemagglutinin (PHA), pokweed mitogen (PWM) and phorbol myristate aceteate/ionomycin (PMA/I). A sandwich ELISA that was able to recognize the recombinant elephant interferon-γ (rEIFN-γ) as well as native interferon-γ from the Asian elephants was performed using anti-elephant IFN-γ rabbit polyclonal antibodies as capture antibodies and biotinylated anti-elephant IFN-γ rabbit polyclonal antibodies as detection antibodies. PMA/I was the best mitogen to use as a positive control for an Asian elephant IGRA. The development of an Asian elephant-specific IGRA that detects native IFN-γ in elephant whole blood provides promising results for its application as a potential diagnostic tool for diseases, such as tuberculosis (TB) in Asian elephants. [ABSTRACT FROM AUTHOR]
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- 2016
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21. A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in Urine
- Author
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Koizumi, Nobuo, primary, Nakajima, Chie, additional, Harunari, Tsunehito, additional, Tanikawa, Tsutomu, additional, Tokiwa, Toshihiro, additional, Uchimura, Eriko, additional, Furuya, Tokujiro, additional, Mingala, Claro Niegos, additional, Villanueva, Marvin Ardeza, additional, Ohnishi, Makoto, additional, and Suzuki, Yasuhiko, additional
- Published
- 2012
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22. A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospiraspp. in Urine
- Author
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Koizumi, Nobuo, Nakajima, Chie, Harunari, Tsunehito, Tanikawa, Tsutomu, Tokiwa, Toshihiro, Uchimura, Eriko, Furuya, Tokujiro, Mingala, Claro Niegos, Villanueva, Marvin Ardeza, Ohnishi, Makoto, and Suzuki, Yasuhiko
- Abstract
ABSTRACTWe developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs, a 16S rRNA gene of pathogenic Leptospiraspp. in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaBnested PCR.
- Published
- 2012
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23. Prevalence and distribution of blaCTX-M, blaSHV, blaTEM genes in extended- spectrum β- lactamase- producing E. coli isolates from broiler farms in the Philippines.
- Author
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Gundran, Romeo S., Cardenio, Paul A., Villanueva, Marvin A., Sison, Fredelon B., Benigno, Carolyn C., Kreausukon, Kwanchai, Pichpol, Duangporn, and Punyapornwithaya, Veerasak
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ENTEROBACTERIACEAE ,BACTERIAL enzymes ,POULTRY farms ,GENES ,AGRICULTURAL exhibitions ,FARMS ,DISEASE prevalence - Abstract
Background: Antimicrobial resistance is a worldwide problem causing serious health threats. Escherichia coli is one of the most important bacteria that causes resistance problem. These bacteria produce an enzyme called extended-spectrum β-lactamase (ESBL) that allows it to become resistant to a wide variety of penicillins and cephalosporins. Currently, no information or published studies on ESBL-producing E.coli in broilers are available in the Philippines. This cross-sectional study was conducted to determine the prevalence and distribution of extended-spectrum β-lactamase (ESBL)-encoding genes, bla
CTX-M , blaSHV , and blaTEM , among E. coli isolates from broiler farms in Luzon, Philippines. Results: Results showed a farm prevalence of 66. 67%. A total of 69 (44.23%) ESBL-producing E. coli were isolated from boot swabs and cloacal swab samples from broiler farms. All major blaCTX-M groups except blaCTX-M-25 group were identified in the isolates. The most prevalent group was blaCTX-M-1 , 72.46% (CI: 60.38–82.54%), followed by blaCTX-M-2 , blaCTX-M-9 group and blaCTX-M-8 . The blaTEM and blaSHV genes were identified in 57.97 and 27.54% of isolates, respectively. The blaCTX-M and blaTEM were the most common gene combinations (33.33%). Coexistence of blaCTX-M types was observed in 50 (73.53%) isolates. Conclusion: This study shows the high prevalence, diversity of patterns and coexistence of ESBL genes in the E. coli isolates from cloacal and boot swabs from broiler farms which pose risks of possible transmission to the environment, other animals and human. [ABSTRACT FROM AUTHOR]- Published
- 2019
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24. Application of Noble Metals in the Advances in Animal Disease Diagnostics
- Author
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Alexis S.P. Tubalinal, Gabriel, G. Lucero, Leonard Paulo, V. Mangahas, Jim Andreus, A. Villanueva, Marvin, and N. Mingala, Claro
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Medical / Veterinary Medicine - Abstract
The advent of molecular biology and biotechnology has given ease and comfort for the screening and detection of different animal diseases caused by bacterial, viral, and fungal pathogens. Furthermore, detection of antibiotics and its residues has advanced in recent years. However, most of the process of animal disease diagnostics is still confined in the laboratory. The next step to conduct surveillance and prevent the spread of animal infectious diseases is to detect these diseases in the field. Through the discovery and continuous development in the field of nanobiotechnology, it was found that incorporation of noble metal nanoparticles to biotechnology tools such as the loop-mediated isothermal amplification (LAMP), lateral flow assays (LFAs) and dipsticks provided a promising start to conduct point-of-care diagnostics. Moreover, the modification and application of nanoparticle noble metals has increased the stability, effectiveness, sensitivity and overall efficacy of these diagnostic tools. Thus, recent advances in disease diagnostics used these noble metals such as gold, silver and platinum.
- Published
- 2022
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