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A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospiraspp. in Urine
- Source :
- Journal of Clinical Microbiology; June 2012, Vol. 50 Issue: 6 p2072-2074, 3p
- Publication Year :
- 2012
-
Abstract
- ABSTRACTWe developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs, a 16S rRNA gene of pathogenic Leptospiraspp. in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaBnested PCR.
Details
- Language :
- English
- ISSN :
- 00951137 and 1098660X
- Volume :
- 50
- Issue :
- 6
- Database :
- Supplemental Index
- Journal :
- Journal of Clinical Microbiology
- Publication Type :
- Periodical
- Accession number :
- ejs27459461
- Full Text :
- https://doi.org/10.1128/JCM.00481-12