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Development and evaluation of an interferon-γ release assay in Asian elephants (Elephas maximus).
- Source :
- Journal of Veterinary Medical Science; Jul2016, Vol. 78 Issue 7, p1117-1121, 5p
- Publication Year :
- 2016
-
Abstract
- We developed an interferon-γ release assay (IGRA) specific for Asian elephants (Elephas maximus). Whole blood collected from forty captive Asian elephants was stimulated with three different mitogens i.e., phytohemagglutinin (PHA), pokweed mitogen (PWM) and phorbol myristate aceteate/ionomycin (PMA/I). A sandwich ELISA that was able to recognize the recombinant elephant interferon-γ (rEIFN-γ) as well as native interferon-γ from the Asian elephants was performed using anti-elephant IFN-γ rabbit polyclonal antibodies as capture antibodies and biotinylated anti-elephant IFN-γ rabbit polyclonal antibodies as detection antibodies. PMA/I was the best mitogen to use as a positive control for an Asian elephant IGRA. The development of an Asian elephant-specific IGRA that detects native IFN-γ in elephant whole blood provides promising results for its application as a potential diagnostic tool for diseases, such as tuberculosis (TB) in Asian elephants. [ABSTRACT FROM AUTHOR]
- Subjects :
- ELEPHANT diseases
IMMUNOLOGICAL blood tests
INTERFERON gamma release tests
Subjects
Details
- Language :
- English
- ISSN :
- 09167250
- Volume :
- 78
- Issue :
- 7
- Database :
- Supplemental Index
- Journal :
- Journal of Veterinary Medical Science
- Publication Type :
- Academic Journal
- Accession number :
- 117346876
- Full Text :
- https://doi.org/10.1292/jvms.15-0701