18 results on '"Rehana Akhter"'
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2. Ethnic meat products of Kashmiri wazwan: a review
- Author
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Sajad A. Rather, F.A. Masoodi, and Rehana Akhter
- Subjects
ethnic ,Kashmiri culture ,meat products ,wazwan ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Wazwan, the Kashmiri cuisine, is a unique and inseparable component of Kashmiri culture. It comprises from seven to 36 dishes of mutton or beef, chicken, fruits, and vegetables. The important ethnic meat products of wazwan include kabab, tabak maaz, aab gosh, rogan josh, nate-yakhni, rista, and goshtaba. The ethnic meat products of Kashmiri wazwan are popular because of their appealing flavor, texture, and palatability characteristics. However, traditional knowledge of these ethnic meat products in other aspects is not carefully documented. As the demand for ethnic/heritage meat products is ever-growing because of rapid urbanization and industrialization, substantial efforts need to be made to meet such increasing requirements. In addition, because of their popularity, there is a vast potential to introduce them at the national level and promote their export. This review aims to describe processing, quality characteristics, underlying problems, and approaches for the development of some important ethnic meat products of Kashmiri wazwan.
- Published
- 2016
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3. Pattern of Contraceptive Use among the Married Couple of Rural Community in Cumilla District
- Author
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Zubaida Ahmed, Meah Monjur Ahmed, Ayet Ali, Shahida Akhter, and Rehana Akhter
- Abstract
Background: Bangladesh has experienced a sevenfold increase in its contraceptive prevalence rate (CPR) in less than forty years from 8% in 1975 to 62% in 2014.Contraceptive practice acceptable moderately in society is said to be associated with socio-economic status, education and other factors. Population stabilization and a gradual lowering of population growth is the basic aim behind contraceptive practice. The population rate of Bangladesh in December, 2018 was 2.01%. The population density is 1252 per km2. Maximum of the population increased in the rural area, because they were not properly used the contraceptive methods. They seldom used contraceptive methods which influenced to increase the population. The purpose of the study is to examine the pattern of contraceptive use among the married couples of the rural area in Bangladesh. The objectives of the study is to determine the extent of awareness regarding contraception among the married persons, to estimate the perception of couples using contraceptive methods, identify the reasons for their adoption & non-adoption and to assess needs of contraceptive methods in rural area. Materials and Methods: It was a descriptive type of cross sectional study, which was carried out in some selected villages at Laksham, Cumilla. Study populations were married women in reproductive age and married man. Purposive sampling was done. Sample size was 333. The number was identified on the basis of assumption. Pretested questionnaire and checklist were used for data collection. Result: It was found that the age range of the respondents were 16 to 70 years, among the 333 respondents 34.2%were 20 to 29 years of age group, 86% were female &14% male.42.6% respondents were educated up to primary level. Majority of the respondents had 10,000 to 20,000 monthly family income. Among 333 respondents majority 28.5% had 3 children. 65.46% of the respondents were house wife, 80.5% respondents were using different types of contraceptive methods, 62.5% were using OCP, 3% IUCD, 3.9% barrier method or condom, 1.5% were in terminal method. 46.8% discontinue their previous contraceptive methods, 11.1% suffered from irregular period and 5.1% weight gain. 68.5% respondents were satisfied with their current contraceptive method, 36% felt need to adopt the contraceptive methods, 18.6% motivated by health workers, 25.2% influenced by the family members and 0.9% by the media. The number of children directly dependent on the educational qualification and monthly family income and are statistically significant (at p
- Published
- 2022
4. Nanoencapsulation of hydroxytyrosol in chitosan crosslinked with sodium bisulfate tandem ultrasonication: Techno-characterization, release and antiproliferative properties
- Author
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Touseef Ahmed Wani, F.A. Masoodi, Rehana Akhter, Towseef Akram, Adil Gani, and Nadeem Shabir
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Chitosan ,Acoustics and Ultrasonics ,Sulfates ,Short Communication ,Organic Chemistry ,Acoustics. Sound ,QC221-246 ,Nanoencapsulation ,Phenylethyl Alcohol ,Antioxidants ,Inorganic Chemistry ,Ionic gelation ,Chemistry ,Nanocapsules ,Chemical Engineering (miscellaneous) ,Environmental Chemistry ,Radiology, Nuclear Medicine and imaging ,Ultrasonics ,Hydroxytyrosol ,Particle Size ,Targeted delivery ,QD1-999 - Abstract
Highlights • Chtosan polymer produces nanocapsules through ionic gelation with sodium bisulfate. • The nanocapsules were amorphous with spherical to irregular shape. • Hydroxytyrosol (HT) was nanoencapsulated using ultrasonication in tandem. • Nanoencapsulated HT was protected during the gastrointestinal simulations. • Drug release was slow and took place mainly during the intestinal simulation., This research includes production of chitosan nanocapsules through ionic gelation with sodium bisulfate for nanoencapsulation of hydroxytyrosol (HT) using ultrasonication in tandem. The resulting nanocapsules encapsulating HT were analyzed for particle size, ζ-potential, packaging characteristics, FESEM, ATR-FTIR, XRD, DSC, in vitro release, antioxidant potential and antiproliferative properties. The nanocapsules (size 119.50–365.21 nm) were spherical to irregular shaped with positive ζ-potential (17.50–18.09 mV). The encapsulation efficiency of 5 mg/g HT (HTS1) and 20 mg/g HT (HTS2) was 77.13% and 56.30%, respectively. The nanocapsules were amorphous in nature with 12.34% to 15.48% crystallinity and crystallite size between 20 nm and 27 nm. Formation of nanocapsules resulted in increasing the glass transition temperature. HTS2 delivered 67.12% HT (HTS1 58.89%) at the end of the simulated gastrointestinal digestion. The nanoencapsulated HT showed higher antioxidant and antiproliferative (against A549 and MDA-MB-231 cancer cell lines) properties than the free HT.
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- 2021
5. Impact of microencapsulated natural antioxidants on the lipid profile and cholesterol oxidation of γ-irradiated meat emulsions
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Rehana Akhter, F.A. Masoodi, Touseef Ahmed Wani, and Sajad Ahmad Rather
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Food Science - Published
- 2022
6. Preparation and characterization of chitosan flake and chitosan nanopowder gels: A comparative study of rheological, thermal and morphological perspectives
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Rehana Akhter, Farooq Ahmad Masoodi, and Touseef Ahmed Wani
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0106 biological sciences ,Materials science ,Rheometry ,Flake ,04 agricultural and veterinary sciences ,040401 food science ,01 natural sciences ,Viscoelasticity ,Chitosan ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Chemical engineering ,chemistry ,Dynamic light scattering ,Rheology ,010608 biotechnology ,High-resolution transmission electron microscopy ,Ball mill ,Food Science - Abstract
Chitosan flakes (CF) were modified to chitosan nanopowder (CP), using planetary ball milling for 8 h. Dynamic light scattering (DLS) revealed and high resolution transmission electron microscopy (HRTEM) confirmed the nano size of CP. Least gelation capacity (LGC) of CF (3%) and CP (4%) was manipulated to fabricate gels with 1.5, 2, and 3 fold increase in LGC. The total color difference was higher in CP than CF gels and depended on concentration. Dynamic rheometry under temperature ramping (20–80 °C and 80-20 °C) showed stable viscoelasticity. Besides, optimal structural network was observed in CP8 and CF6. During temperature ramping, the damping factor (tan δ) for all the gels was >0.1
- Published
- 2021
7. Subcritical treatment of olive oil: Minor phenolic composition and antioxidant properties of the solvent extracts
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O.C. Sharma, Farooq Ahmad Masoodi, Touseef Ahmed Wani, Mohd Masarat Dar, and Rehana Akhter
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0106 biological sciences ,Aqueous solution ,Antioxidant ,Chromatography ,Chemistry ,medicine.medical_treatment ,Extraction (chemistry) ,Water extraction ,04 agricultural and veterinary sciences ,040401 food science ,01 natural sciences ,Solvent ,Tyrosol ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Oleuropein ,010608 biotechnology ,medicine ,Hydroxytyrosol ,Food Science - Abstract
Olive oil extracted from four exotic olive cultivars was subjected to subcritical treatment using green and organic solvents. The minor phenolic compounds were identified and quantified by RP–HPLC–DAD. Significant (p ≤ 0.05) variation in the phenolic compounds and antioxidant properties was observed with respect to cultivar, solvent and the extraction conditions. For the methanolic and ethanolic extracts, the antioxidant properties generally increased upon subcritical treatment but decreased drastically for subcritical water extraction. Besides correlation among hydroxytyrosol, tyrosol and oleuropein, the multivariate analysis revealed high correlation of antioxidant properties with hydroxytyrosol, oleuropein, tyrosol and apigenin. The principal components analysis (PCA) revealed two components explaining 86.6% variability. PCA separated aqueous and organic solvents both at ambient and subcritical conditions in the score plot. The loading plot exhibited maximum positive loadings of the analyses. It is concluded that subcritical water extraction could be manipulated for the recovery of oleuropein and hydroxytyrosol from olive oil.
- Published
- 2021
8. Effect of double alginate microencapsulation on in vitro digestibility and thermal tolerance of Lactobacillus plantarum NCDC201 and L. casei NCDC297
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Sajad A. Rather, Farooq Ahmad Masoodi, Adil Gani, Rehana Akhter, and Sajad Mohd Wani
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education.field_of_study ,biology ,Chemistry ,Population ,Spherical morphology ,chemistry.chemical_element ,04 agricultural and veterinary sciences ,02 engineering and technology ,Calcium ,021001 nanoscience & nanotechnology ,biology.organism_classification ,040401 food science ,In vitro ,law.invention ,Probiotic ,0404 agricultural biotechnology ,law ,Food science ,0210 nano-technology ,education ,Incubation ,Bacteria ,Lactobacillus plantarum ,Food Science - Abstract
This study was undertaken for the microencapsulation of Lactobacillus plantarum NCDC201 and L. casei NCDC297 into double alginate coatings. Microencapsulated probiotics showed significant improvement in their survivability after simulated gastrointestinal passage and exposure to heat treatments. The resistance in simulated gastric juice (SGJ) (120 min) was 47.50% and 45.82% higher as compared to free cells of L. plantarum NCDC201 and L. casei NCDC297, respectively. After incubation in simulated intestinal juice (SIJ) (120 min), the viable probiotic population was 6.34 log CFU/ml and 6.92 log CFU/ml for microencapsulated L. plantarum NCDC201 and L. casei NCDC297, respectively. Similarly, microencapsulated probiotics showed relevant counts at higher heat exposure (75 °C for 1 and 10 min). SEM results indicated the absence of free bacteria confirming the formation of microcapsules, with spherical morphology, continuous and compact surfaces. ATR-FTIR analysis confirmed the cross linking of the microcapsules by calcium chloride and successful immobilization of the probiotics into the polymer microcapsules. DSC suggested the formation of cross-linking and structure of “egg box” and increase in the melting temperature of microcapsules. This study has concluded that double alginate coating technique enhanced the stability of probiotics at high temperature (75 ± 1 °C) and in simulated gastric and intestinal conditions.
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- 2017
9. Advances in use of natural antioxidants as food additives for improving the oxidative stability of meat Products
- Author
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Rehana Akhter, Sajad A. Rather, Jahangir A. Rather, Farooq Ahmad Masoodi, and Khurshid A Shiekh
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food.ingredient ,food ,Chemistry ,Food additive ,Food science - Published
- 2016
10. Impact of thermal processing and storage on fatty acid composition and cholesterol oxidation of canned traditional low-fat meat product of India
- Author
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Jahangir A. Rather, Farooq Ahmad Masoodi, Adil Gani, Rehana Akhter, Sajad A. Rather, Tariq Ahmad Ganaie, and Sajad Mohd Wani
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0106 biological sciences ,chemistry.chemical_classification ,Cholesterol ,Linoleic acid ,food and beverages ,Vaccenic acid ,04 agricultural and veterinary sciences ,040401 food science ,01 natural sciences ,Palmitic acid ,chemistry.chemical_compound ,0404 agricultural biotechnology ,chemistry ,010608 biotechnology ,Meat emulsion ,lipids (amino acids, peptides, and proteins) ,Composition (visual arts) ,Stearic acid ,Food science ,Food Science ,Polyunsaturated fatty acid - Abstract
The effects of thermal processing/canning and storage on fatty acids (FAs) contents and cholesterol oxidation of traditional low-fat meat product (goshtaba) of Jammu and Kashmir (JK India prepared from meat emulsion with added fat (20–30%), salt, spices and condiments and cooked in the curd based gravy. The FAs composition i.e. SFAs, MUFAs, PUFAs and TFAs during processing and storage showed non-significant difference in all goshtaba products (P > 0.05). The dominant FAs were C16:0 (palmitic acid) and C18:0 (stearic acid) among SFAs, C18:1n9 (n-9-oleic acid) among MUFAs, C18:2n6 (n-6- linoleic acid) followed by C18:3n3 (n-3-α-linolenic acid) among PUFAs and C18:1t11 (vaccenic acid) among the TFAs in all products. The cholesterol content decreased significantly after thermal processing in all products (P
- Published
- 2021
11. A review on gestational trophoblastic disease
- Author
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Romena Afroz, Kashfia Ahmed Keya, Afroza Khanom, Zillur Rahman Bhuiyan, Shirin Akter Begum, and Rehana Akhter
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Gynecology ,Molar ,medicine.medical_specialty ,Obstetrics ,business.industry ,Gestational trophoblastic disease ,Choriocarcinoma ,General Medicine ,030204 cardiovascular system & hematology ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,Molar pregnancy ,stomatognathic system ,embryonic structures ,Mole ,medicine ,Gestation ,030212 general & internal medicine ,Complete Mole ,business ,Placental site trophoblastic tumor - Abstract
Molar pregnancy occurs when the fertilization of the egg by the sperm goes wrong and leads to the growth of abnormal cells or clusters of water filled sacs inside the womb. This condition is one of a group of conditions known as gestational trophoblastic tumours (GTTs). Molar pregnancies used to be called hydatidiform mole but now most people call them molar pregnancies. Molar pregnancies are rare but they are the most common type of gestational trophoblastic tumour. In the UK, about 1 in 590 pregnancies is a molar pregnancy. In Asian women, molar pregnancies are about twice as common as in Caucasian women. Most molar pregnancies are benign. They can spread beyond the womb in some women, but are still curable. Molar pregnancies can either be complete or partial. In case of complete mole, no parts of foetal tissue are formed. In case of partial mole there may be some foetal tissue in the womb, alongside the molar tissue. By measuring the levels of ?hCG in blood and urine in high dilution helps to diagnose a molar pregnancy; an ultrasound scan can also diagnose many women with molar pregnancy. The molar tissue needs to be surgically removed. Afterwards, in around 10 to 15 out of 100 women, some molar tissue remains in the deeper tissues of the womb or other parts of the body. This is called a persistent gestational tumour. Invasive mole, choriocarcinoma, and placental site trophoblastic tumor (PSTT) termed as gestational trophoblastic neoplasia (GTN), which can progress, invade, metastasize, and lead to death if left untreated.These women need to have chemotherapy completely get rid of the abnormal cells.Bangladesh Med J. 2015 Jan; 44 (1): 51-56
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- 2016
12. The Role of HbA1C for Diagnosis of Type-2 Diabetes Mellitus - A Review
- Author
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Shahana Zabeen and Sultana Rehana Akhter
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medicine.medical_specialty ,Pediatrics ,business.industry ,Diabetes mellitus ,medicine ,Random blood glucose ,Type 2 Diabetes Mellitus ,medicine.disease ,business ,Surgery - Abstract
For many years, the diagnosis of diabetes has been made through the laboratory- based measurement of fasting or random blood glucose levels or using OGTT. In the case of diabetes, the major outcome of interest is long term micro vascular complications for which a large body of data has been accumulated leading to the endorsement of HbA1C for diagnosis in many countries worldwide, with some variations in cut-offs and testing strategies.Faridpur Med. Coll. J. Jan 2016;11(1): 30-32
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- 2016
13. Xanthan gum as a fat replacer in goshtaba-a traditional meat product of India: effects on quality and oxidative stability
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A. H. Malik, Farooq Ahmad Masoodi, Adil Gani, Rehana Akhter, Sajad A. Rather, and S. M. Wani
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Antioxidant ,Chemistry ,medicine.medical_treatment ,Protein oxidation ,Chewiness ,medicine ,Meat emulsion ,High fat ,TBARS ,Original Article ,Food science ,Quality characteristics ,Xanthan gum ,Food Science ,medicine.drug - Abstract
Goshtaba is a restructured meat product of Kashmiri wazwan prepared from meat emulsion with added fat (20 %), salt, spices and condiments and cooked in the curd. The present study was undertaken for the development of low fat goshtaba with the addition of xanthan gum as a fat replacer and was evaluated for proximate composition, pH, colour, lipid and protein oxidation, texture, microstructure and sensory properties. Low fat goshtaba formulations containing xanthan gum were higher in protein and moisture contents but, lower in fat content and pH value than the high fat control (p
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- 2015
14. Evaluation of breast disease by clinical breast examination (CBE): Experience of a tertiary care hospital
- Author
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Masuma Amanullah, Farzana Deeba, Jesmin Banu, Mir Mosarraf Hossain, Begum Nasreen, and Rehana Akhter
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medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Obstetrics ,Cross-sectional study ,Breast lumps ,Cancer ,medicine.disease ,Surgery ,Nipple discharge ,Breast cancer screening ,Breast cancer ,Obstetrics and gynaecology ,medicine ,Breast disease ,medicine.symptom ,business - Abstract
Introduction: Breast cancer is the second most common cancer (10.4 % of all cancer incidence, both sexes counted) and the fifth most common cause of cancer death in the world. In 2005, breast cancer caused 502,000 deaths worldwide. Clinical evaluation could function as a valuable diagnostic tool. Clinical evaluation, however, is a simple method to detect cases as it is inexpensive and non-invasive and if found to be accurate, might be of great value. Aim: To evaluate patients referred to colposcopy clinic in BSMMU for breast symptoms by clinical breast examination (CBE). Material and method: The study design was cross sectional study done in the colposcopy clinic in the department of Obstetrics and Gynaecology, under the ‘Establishment of National centre for cervical and breast cancer screening and training at BSMMU’ and ‘Cervical and breast cancer screening and training in BSMMU’ projects. Result : A total of 752 patients fulfilled the inclusion criteria , demographic features are demonstrated in mean age of the patients was 35.1±9.2 years, mean BMI was 22.5±2.1, about 30% subjects were postmenopausal and about 2% patients had history of breast cancer. Presenting complaint was a self-detected breast mass and found to have a mass on examination by her physician in 204 patients. 308 subjects complained of mastalgia; 34 patients complained of nipple discharge. Only 5 patients complained of axillary lymph node enlargement. Nipple abnormalities like retracted and cracked nipple was complained by 4% cases. Most patients (58%) were found to have normal findings. Palpable mass was found in 121 cases and bilateral in one case. Nipple abnormalities found in 10 cases. Tenderness and nipple discharge found in 15% and 4% cases respectively. Regarding the characteristics of the masses proved by cytology (FNAC), 109 were benign and 12 were malignant. Conclusion : CBE is an important screening procedure to identify breast pathology. Out of 752 patients breast pathology were identified in about 41% of patients and 10% breast lumps were malignant. So CBE should be done every three yearly in
- Published
- 2015
15. Application of guar–xanthan gum mixture as a partial fat replacer in meat emulsions
- Author
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A. H. Malik, Adil Gani, Rehana Akhter, Sajad A. Rather, Sajad Mohd Wani, Farooq Ahmad Masoodi, and Jahangir A. Rather
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0301 basic medicine ,030109 nutrition & dietetics ,Chromatography ,Oxidative degradation ,Chemistry ,Fat substitute ,Guar ,04 agricultural and veterinary sciences ,040401 food science ,03 medical and health sciences ,0404 agricultural biotechnology ,Emulsion ,medicine ,High fat ,Meat emulsion ,TBARS ,Original Article ,Food science ,Xanthan gum ,Food Science ,medicine.drug - Abstract
The physicochemical, oxidative, texture and microstructure properties were evaluated for low fat meat emulsions containing varying levels of guar/xanthan gum mixture (1:1 ratio) as a fat substitute. Partial replacement of fat with guar/xanthan gum resulted in higher emulsion stability and cooking yield but lower penetration force. Proximate composition revealed that high fat control had significantly higher fat and lower moisture content due to the difference in basic formulation. Colour evaluation revealed that low fat formulations containing gum mixture had significantly lower lightness and higher yellowness values than high fat control formulation. However non-significant difference was observed in redness values between low fat formulations and the high fat control. The pH values of the low fat formulations containing gum mixture were lower than the control formulations (T0 and TC). The MetMb% of the high fat emulsion formulation was higher than low fat formulations. The significant increase of TBARS value, protein carbonyl groups and loss of protein sulphydryl groups in high fat formulation reflect the more oxidative degradation of lipids and muscle proteins during the preparation of meat emulsion than low fat formulations. The SEM showed a porous matrix in the treatments containing gum mixture. Thus, the guar/xanthan gum mixture improved the physicochemical and oxidative quality of low fat meat emulsions than the control formulations.
- Published
- 2016
16. THE EMERGENT CONCERN OF SEROPOSITIVE STATUS OF HEPATITIS-B VIRUS AND HEPATITIS-C VIRUS IN THE PREGNANT FEMALES ATTENDING A TERTIARY CARE HOSPITAL
- Author
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Kaweeta, Kumari, Naresh Kumar, Seetlani, and Rehana, Akhter
- Subjects
Adult ,Hepatitis B virus ,Hepatitis B Surface Antigens ,Infant ,Enzyme-Linked Immunosorbent Assay ,Hepacivirus ,Hepatitis C Antibodies ,Delivery, Obstetric ,Hepatitis B ,Hepatitis C ,Tertiary Care Centers ,Cross-Sectional Studies ,Pregnancy ,Risk Factors ,Prevalence ,Humans ,Female ,Pakistan ,Pregnancy Complications, Infectious ,Follow-Up Studies ,Retrospective Studies - Abstract
Viral hepatitis is a global problem affecting millions of people including pregnant females. Viral hepatitis during pregnancy is associated with both maternal and neonatal mortality and morbidity. This study was an attempt to assess the seropositive status of hepatitis-B and C infection among pregnant women in Karachi, Pakistan.This cross sectional observational study was conducted at Sir Syed College of Medical Sciences and Trust Hospital, Karachi from January to September 2012. Patients were recruited by consecutive sampling. At the booking visit, blood was drawn and tested for HbsAg and Anti HCV by Eliza method.Among the screened population, 2% were reactive for HBV and 13.3% were found reactive for HCV. All HbsAg and HCV positive pregnant patients had one or more than one delivery.In our study sample, high frequency of HBV and HCV is suggestive of the importance of antenatal screening of these viral diseases, which has impact on the mother as well as the new born baby. HCV was more common as compared to HBV which is quite alarming.
- Published
- 2015
17. Controlled Evaluation of Bactec Peds Plus/F and Bactec Lytic/10 Anaerobic/F Media for Isolation of Salmonella enterica Serovars Typhi and Paratyphi A from Blood
- Author
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Megan E. Reller, Shahida Qureshi, Shazia Sultana, Zulfiqar A Bhutta, Anita K. M. Zaidi, Beenish Hanif, Rumina Hasan, Shazia Azeem, Donald A. Goldmann, and Rehana Akhter
- Subjects
Microbiology (medical) ,Serotype ,Time Factors ,Adolescent ,Nalidixic acid ,Salmonella typhi ,Sensitivity and Specificity ,Microbiology ,Paratyphoid Fever ,medicine ,Humans ,Blood culture ,Anaerobiosis ,Typhoid Fever ,Child ,Bacteriological Techniques ,biology ,medicine.diagnostic_test ,business.industry ,Chloramphenicol ,Salmonella paratyphi A ,Infant ,Bacteriology ,biology.organism_classification ,Trimethoprim ,Aerobiosis ,Culture Media ,Blood ,Salmonella enterica ,Child, Preschool ,business ,medicine.drug - Abstract
We compared anaerobic lytic (AL) and pediatric aerobic resin-containing (Peds Plus/F) blood culture media for the isolation of Salmonella enterica serotype Typhi or Paratyphi A from children. The yields from AL and Peds Plus/F media were the same with equal volumes of blood, but recovery was faster from AL medium than Peds Plus/F medium (10.7 and 16.4 h, respectively) ( P < 0.001).
- Published
- 2009
18. Inheritance of azetidine-2-carboxylic acid resistance in Arabidopsis thaliana.
- Author
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Katterman, Frank R., Lehle, Fredric R., Ray, Dennis T., Smith, Steven E., Khan, Rehana Akhter., Katterman, Frank R., Lehle, Fredric R., Ray, Dennis T., Smith, Steven E., and Khan, Rehana Akhter.
- Abstract
A number of hypotheses link salt tolerance in plants to proline accumulation or transport of proline. To begin to understand the genetic basis of this correlation, fifteen mutants of Arabidopsis thaliana were selected for resistance to the toxic proline analog, azetidine-2-carboxylic acid (ACA). These mutants were characterized by seedling growth and proline content on nutrient agar media in the absence and presence of ACA and NaCl. One of these ACA-resistant mutants, KG3, also showed enhanced tolerance to NaCl and was characterized by a recessive trait, transparent testa. Inheritance studies indicated that ACA resistance in KG3 was due to a single recessive gene mutation, named aca1. Genetic mapping studies were done by crossing KG3 with a morphological marker line W100 to determine the chromosomal location of ACa resistance in relation to known markers. Segregation analysis of 180 single-seed-descent F₃ families showed that aca1 was linked to marker tt3. Marker tt3 is located on chromosome V of Arabidopsis thaliana. Segregation of tt3 and aca1 did not show a 9:3:3:1 ratio, suggesting that aca1 was closely linked to tt3, located 62.1 cM from the end of chromosome V. The transparent testa phenotype of KG3 was complemented by locus tt4 also located on this chromosome. To determine the basis of enhanced NaCl tolerance in KG3, F₃ families from a cross between KG3 and Columbia pubescent wild type were tested for NaCl resistance. Families showing optimal growth after release from salt stress were scored for NaCl tolerance. Segregation analysis indicated that the salt tolerance in KG3 was due to a single recessive gene mutation called salt addicted (sad1). The sad1 phenotype appeared to have required NaCl for optimal growth. Segregation analysis of aca1 and sad1 phenotype showed that they were not linked. Molecular mapping of aca-1 was done by using a number of RFLP markers selected from all five Arabidopsis thaliana chromosomes. This study indicated that aca1 was linked
- Published
- 1993
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