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Il contributo presenta alcune azioni in corso all’interno del progetto europeo INES (http://ines.unibo.it/), finanziato nell’ambito E+ Cooperation Partnership in School Education, coordinato dall’Università di Bologna (2021-2024). Il progetto si propone di realizzare Risorse Educative Aperte (OERs) e interventi formativi per ridurre il problema della droga, in particolare delle NPS, nel contesto della scuola secondaria di secondo grado. Il progetto ha una matrice fortemente interdisciplinare e si fonda su un costante lavoro di collaborazione tra ricercatori, insegnanti e studenti ed esperti esterni.

A sensitive and accurate method for the spectrofluorimetric determination of trace levels of aluminum in hemodialysis solutions using Mordant Red 19 as the complexation reagent has been developed. The optimal experimental conditions for the concentration of fluorimetric reagent, pH, temperature, and the specific type of matrix are reported. The emission of the fluorescent metal chelate was measured at 555 nm, excitation at 478 nm. Linearity between emission intensity and aluminum concentration was found in the 2-20 ppb range in standard aluminum solutions. Limit of detection was 0.4 ppb. The aluminum amounts in some commercial hemodialysis solutions were determined by means of the extrapolation method. The proposed method proved to be suitable in terms of sensitivity and accuracy for the determination of aluminum in dialysis fluids.

This brief proposes a new multiple input multiple output (MIMO) control for off-board electric vehicle (EV) dc fast chargers. The proposed feedback matrix design avoids multiple tuning of controllers in multiple and interconnected loops while improving the performance of interleaved dc buck converters over classical PI/PID controls. The innovative features of the presented strategy are the reference current monotonic tracking from any initial state of charge with an arbitrarily fast settling time and the fast compensation of both load variations and imbalances among the legs. Numerical results validate the performance improvements of the proposed discrete-time MIMO algorithm for interleaved buck converters over classical PI/PID controls. Full-scale hardware-in-the-loop (HIL) and scaled-down prototype experimental results prove the feasibility and effectiveness of the proposal.

Three-phase, four-wire, split-capacitor inverters, thanks to their capability to deal with unbalanced systems, are currently employed in photovoltaic installations, electric vehicles battery chargers, active power filters, and many other grid-tied applications. The minimization of ac output current ripple and switching losses positively impacts the inverter's efficiency, volume, weight, and cost optimization. For this reason, a novel variable switching frequency driving strategy independently tunable on each phase is proposed in this article. Taking advantage of phase current ripple prediction, a proper variable switching frequency strategy is applied for obtaining a flat current ripple profile. Having tuned the driving strategy parameters, it is possible to optimize and compare individual metrics such as the maximum peak-to-peak value of the current ripple, current ripple rms, average switching frequency, and switching losses. By applying the proposed modulation method, a significant inverter performance enhancement has been obtained. Converter efficiency is improved without introducing detrimental effects on the current harmonic quality. Analytical derivations are expressed as a modulating index function and the power factor for balanced and unbalanced systems. All the theoretical developments are verified throughout numerical simulations and experimental tests.

A new Modular Multilevel Converter with Interleaved half-bridge Sub-Modules (ISM-MMC) is proposed in this paper. The ISM-MMC exhibits a higher modularity and scalability in terms of current ratings with respect to a conventional MMC, while preserves the typical voltage level adaptiveness. The ISM-MMC brings the known advantages of classical MMC to low-voltage, high-current applications making it a novel candidate for the sector of ultra-fast chargers for all types of electrical vehicles (EV). This advanced topology makes it possible to easily reach charging power of the EV charging system up to 4.5 MW and beyond with low-voltage supply. To operate the new converter, a hybrid modulation scheme that helps to exploit advantages of the interleaving scheme, is implemented, and explained in this paper. It has been verified that the typical MMC control methods are still applicable for ISM-MMC. A comparative study between classical MMC and ISM-MMC configurations in terms of output characteristics and efficiency is also given. Furthermore, it has been demonstrated that the number of ac voltage levels is synthetically multiplied by the number of interleaved half- bridge legs in submodules. Simulations, Hardware-in-the-Loop and Experimental tests are carried out to demonstrate the feasibility of the proposed topology and implemented modulation scheme.

A new state observer-based current balancing method for Modular Multilevel Converters with Interleaved half-bridge Sub-Modules (ISM-MMC) is presented in this paper. The developed observer allows estimating currents through interleaved half-bridge legs in each submodule of ISM-MMC basing only on arm current and submodules capacitor voltage measurements. Then, the interleaved current balancing control uses the estimated currents to reduce the interleaved currents imbalance caused by upstream control actions. This technique minimizes the number of required current sensors in ISM-MMC, thereby reducing the converter's cost, weight, and volume. Capabilities of the proposed sensorless interleaved currents balancing control has been tested against standard parameter tolerances of the composing passive elements. In addition to that, a novel capacitor voltage balancing strategy for MMCs is developed. The new algorithm contains main advantages of the classical sorting-based capacitor voltage balancing methods while provides an opportunity to decouple two balancing tasks of ISM-MMC, namely capacitor voltage and interleaved legs current balancing. The feasibility of the proposed methods is verified by extensive simulation and experimental tests on a laboratory prototype by the corresponding system response under the output characteristics variation and interleaved current control perturbation.

In the context of smart cities, direct current overhead contact lines, usually adopted to power urban transportation systems such as trolleybuses, tramways, metros, and railways, can serve as a backbone to connect different modern emerging technologies. Among these, in-motion charging (IMC) trolleybuses with on-board batteries are expected to be very impactful on the DC network’s power flow and may require specific voltage and current control. These factors motivate the development of a simulation tool able to emulate these devices’ absorption and their effect on the supply infrastructure. The main innovative value of the work is to improve a simulation model of a trolleybus grid through a data-driven approach by using measurements of voltage and current output from a traction substation. The measurements are essential for understanding the behavior of vehicle weight variation throughout the day. Thanks to this information, a characterization of the current draw by conventional trolleybuses and IMC trolleybuses is then provided for each trolleybus route in a specific power section of the Bologna trolleybus system. By integrating the variation in vehicle weight within the model, a simulation of a possible daily operation of a trolleybus feeding section has been performed, obtaining a 7% error between the daily energy calculated from the simulation and that obtained through measurements. This analysis demonstrates the feasibility of the adopted simulation tool, which can also be used to evaluate additional hypothetical trolleybus operation scenarios. One of these possible scenarios considers IMC vehicles, and it is also evaluated in this paper.

An off-board dc fast battery charger for electric vehicles (EVs) with an original control strategy aimed to provide ripple-free output current in the typical EV batteries voltage range is presented in this article. The proposed configuration is based on modular three-phase interleaved converters and supplied by the low-voltage ac grid. The ac/dc interleaved three-phase active rectifier is composed of three standard two-level three-phase converter modules with a possibility to slightly adjust the dc-link voltage level in order to null the output current ripple. A modular interleaved dc/dc converter, formed by the same three-phase converter modules connected in parallel, is used as an interface between the dc link and the battery. The use of low-cost, standard and industry-recognized three-phase power modules for high-power fast EV charging stations enables the reduction of capital and maintenance costs of the charging facilities. The effect of coupling on the individual input/output inductors and total input/output current ripples has been investigated as well, considering both possible coupling implementations, i.e., inverse and direct coupling. Numerical simulations are reported to confirm the feasibility and the effectiveness of the whole EV fast charging configuration, including the proposed control strategy aimed to null the ripple of the output current. Experimental results are provided by a reduced scale prototype of the output stage to verify the ripple-free output current operation capability.

This paper presents a comprehensive study of peak-to-peak and root-mean-square (RMS) values of AC current ripples with balanced and unbalanced fundamental currents in a generic case of three-phase four-leg converters with uncoupled AC interface inductors present in all three phases and in neutral. The AC current ripple characteristics were determined for both phase and neutral currents, considering the sinusoidal pulse-width modulation (SPWM) method. The derived expressions are simple, effective, and ready for accurate AC current ripple calculations in three- or four-leg converters. This is particularly handy in the converter design process, since there is no need for heavy numerical simulations to determine an optimal set of design parameters, such as switching frequency and line inductances, based on the grid code or load restrictions in terms of AC current ripple. Particular attention has been paid to the performance comparison between the conventional three-phase three-leg converter and its four-leg counterpart, with distinct line inductance values in the neutral wire. In addition to that, a design example was performed to demonstrate the power of the derived equations. Numerical simulations and extensive experimental tests were thoroughly verified the analytical developments.

Three-phase four-leg voltage-source converters have been considered for some recent projects in smart grids and in the automotive industry, projects such as on-board electric vehicles (EVs) chargers, thanks to their built-in ability to handle unbalanced AC currents through the 4th wire (neutral). Although conventional carrier-based modulations (CBMs) and space vector modulations (SVMs) have been commonly applied and extensively studied for three-phase four-leg voltage-source converters, very little has been reported concerning their pollution impact on AC grid in terms of switching ripple currents. This paper introduces a thorough analytical derivation of peak-to-peak and RMS values of the AC current ripple under balanced and unbalanced working conditions, in the case of three-phase four-leg converters with uncoupled AC-link inductors. The proposed mathematical approach covers both phase and neutral currents. All analytical findings have been applied to two industry recognized CBM methods, namely sinusoidal pulse-width modulation (PWM) and centered PWM (equivalent to SVM). The derived equations are effective, simple, and ready-to-use for accurate AC current ripple calculations. At the same time, the proposed equations and diagrams can be successfully adopted to design the conversion system basing on the grid codes in terms of current ripple (or total harmonic distortion (THD)/total demand distortion (TDD)) restrictions, enabling the sizing of AC-link inductors and the determination of the proper switching frequency for the given operating conditions. The analytical developments have been thoroughly verified by numerical simulations in MATLAB/Simulink and by extensive experimental tests.

This paper proposes a full-hybrid driveline based on an electric continuously variable transmission (e-CVT), which is inspired by the car industry’s most successful solution. The paper describes the operating principle, the system architecture, and the control scheme of the proposed driveline. An analysis of four possible operating modes shows that the e-CVT driveline leads to a performance similar to that of conventional tractors, as well as unusual features such as power boost, full-electric mode, optimized auxiliary drive and electric power delivery capability. The compact layout proposed for the e-CVT also makes it possible to simplify the overall layout of the tractor, particularly during the installation of both the thermal engine and the cooling system.

Electrical power grids are rapidly evolving into smart grids, with smart homes also making an important contribution to this. In fact, the well-known and emerging technologies of renewables, energy storage systems and electric mobility are each time more distributed throughout the power grid and included in smart homes. In such circumstances, since these technologies are natively operating in DC, it is predictable for a revolution in the electrical grid craving a convergence to DC grids. Nevertheless, traditional loads natively operating in AC will continue to be used, highlighting the importance of hybrid AC/DC grids. Considering this new paradigm, this paper has as main innovation points the proposed control algorithms regarding the role of front-end AC/DC converters in hybrid AC/DC smart homes, demonstrating their importance for providing unipolar or bipolar DC grids for interfacing native DC technologies, such as renewables and electric mobility, including concerns regarding the power quality from a smart grid point of view. Furthermore, the paper presents a clear description of the proposed control algorithms, aligned with distinct possibilities of complementary operation of front-end AC/DC converters in the perspective of smart homes framed within smart grids, e.g., enabling the control of smart homes in a coordinated way. The analysis and experimental results confirmed the suitability of the proposed innovative operation modes for hybrid AC/DC smart homes, based on two different AC/DC converters in the experimental validation., This work has been supported by FCT - Fundação para a Ciência e Tecnologia within the R&D Units Project Scope: UIDB/00319/2020. This work has been supported by the FCT Project newERA4GRIDs PTDC/EEI-EEE/30283/2017, and by the FCT Project DAIPESEV PTDC/EEIEEE/30382/2017.

Volumetric absorptive microsampling (VAMS) and dried urine spot (DUS) strategies were applied for the collection of dried microsamples for anti-doping testing of low-stability peptide hormones and growth factors prohibited by the World Anti-Doping Agency (WADA). Drying, storage and transport conditions, as well as pretreatment steps, were optimised before liquid chromatography - tandem mass spectrometry (LC–MS/MS) analysis. The analytical method has been fully validated in terms of sensitivity (limits of quantitation 0.3−10 ng/mL), precision (RSD% < 6.6 %) and extraction yields (78–91 %). Dried microsample stability studies (90 days) have been performed and compared to fluid urine stability. Significantly higher losses have been observed in fluid urine stored at −20 °C (up to 55 %) and −80 °C (up to 29 %) than in dried urine microsamples stored at room temperature (< 19 %). The final microsampling and analysis protocols allow the collection of urine microvolumes, unlikely to be tampered, stably storable and shippable with no particular precautions for possible anti-doping testing of prohibited peptides and hormones.

This paper presents a thorough prediction of DC-link voltage switching ripples in the three-phase four-leg inverters operating in balanced and unbalanced working conditions. The unbalanced modes examined here employ the highest degree of AC current imbalance while still preserving three-phase operation. This behavior can be found in many grid-connected or standalone grid-forming three-phase converters that supply “heavy” single-phase loads, comprising a recent trend in smart-grid, smart electric vehicle (EV)-charging applications. In this sense, for instance, the smart EV chargers might be employed in conditions when different power is drawn/injected from/to the grid, providing power conditioning services to the latter. The analysis of three-phase four-leg inverters is then extended to single-phase operations typical of home-charging or vehicle-to-home (V2H) applications. Their performances in terms of DC-link voltage switching ripple are demonstrated. Two of the most common carrier-based PWM modulation techniques are employed to drive the three-phase inverter—namely, sinusoidal PWM and centered PWM (carrier-based analogy of the space vector modulation). The derived mathematical expressions of peak-to-peak and RMS values of DC-link voltage switching ripple for balanced and unbalanced conditions are handy for designing the associated DC-link capacitor and estimating the overall efficiency of the converter. Extensive numerical simulations and experimental tests have been performed to validate the presented analytical developments.

Trolleybus systems are resurfacing as a steppingstone to carbon-neutral urban transport. With an eye on smart city evolution, the study and simulation of a proper monitoring system for trolleybus infrastructures will be essential. This paper merges the authors’ engineering knowledge and sources available in the literature on designing and modeling catenary-based electric traction networks and performs a critical review of them to lay the foundations for proposing possible optimal alternatives. A novel multi-vehicle motion-based model of the DC catenary system is then devised and simulated in Matlab-Simulink, which could prove useful in predicting possible technical obstacles arising from the next-future introduction of smart electric traction grids, inevitably featuring greater morphological intricacy. The modularity property characterizing the created model allows an accurate, detailed, and flexible simulation of sophisticated catenary systems. By means of graphical and numerical results illustrating the behavior of the main electrical line parameters, the presented approach demonstrates today’s obsolescence of conventional design methods used so far. The trolleybus network of the city of Bologna was chosen as a case study.

Therapeutic drug monitoring (TDM) is an important tool for correlating the administered drug dose to drug and metabolite concentrations in the body and to therapeutic and adverse effects. In the case of treatment with drugs active on the central nervous system (CNS), frequent TDM becomes really useful, especially for patient compliance checking and for therapy optimisation. The selective serotonin reuptake inhibitors (SSRIs) fluoxetine and sertraline, chosen as target compounds for this study, are two antidepressants mainly used for major depression, but also for obsessive-compulsive disorder associated with neurodegenerative diseases and for eating disorders. Microsampling approaches can be used to make TDM patient-friendly, by means of minimally invasive fingerpricking instead of classic invasive venipuncture. In this study, an innovative volumetric microsampling approach based on the use of hemaPEN technology is proposed to simultaneously obtain four identical dried whole blood microsamples by means of a single capillary sampling. The developed strategy shows significant advantages in terms of blood collection and storage, fast and feasible extraction procedure and sensitive LC-MS/MS analysis, also providing satisfactory validation results (extraction yield >81%, RSD

Testing and monitoring anabolic androgenic steroids in biological fluids is a key activity in anti-doping practices. In this study, a novel approach is proposed, based on dried urine microsampling through two different workflows: dried urine spots (DUS) and volumetric absorptive microsampling (VAMS). Both techniques can overcome some common drawbacks of urine sampling, such as analyte instability and storage and transportation problems. Using an original, validated liquid chromatography&ndash, tandem mass spectrometry (LC-MS/MS) method, exogenous and endogenous unconjugated steroids were analysed. Despite the limitations of microsampling volume, good sensitivity was obtained (limit of quantitation &le, 1.5 ng/mL for all analytes), with satisfactory precision (relative standard deviation &lt, 7.6%) and absolute recovery (&gt, 70.3%). Both microsampling platforms provide reliable results, in good agreement with those obtained from urine.

Sweet cherries (Prunus avium L.) are highly appreciated fruits for their taste, color, nutritional value, and beneficial health effects. In this work, seven new cultivars of sweet cherry were investigated for their main quality traits and nutraceutical value. The phytochemical profile of three classes of phenolic compounds and the antioxidant activity of the new cultivars were investigated through high-performance liquid chromatography with diode array detection (HPLC-DAD) and spectrophotometric assays, respectively, and compared with those of commonly commercialized cultivars. Cyanidine-3-O-rutinoside was the main anthocyanin in all genotypes, and its levels in some new cultivars were about three-fold higher than in commercial ones. The ORAC-assayed antioxidant capacity was positively correlated with the total anthocyanin index. The nutraceutical value of the new cultivars was investigated in terms of antioxidant/neuroprotective capacity in neuron-like SH-SY5Y cells. Results demonstrated that the new cultivars were more effective in counteracting oxidative stress and were also able to upregulate brain-derived neurotrophic factor (BDNF), a pro-survival neurotrophin, suggesting their potential pleiotropic role in counteracting neurodegenerations.

Graphical abstract, Highlights • SARS-CoV-2 emergency sparks the need for diagnostic and therapeutic actions. • Microsampling is emerging in as an attractive alternative to traditional sampling. • Advantages and challenges of the main microsampling techniques are reported. • Available microsampling applications of interest for SARS-CoV-2 are described. • Most useful information for researchers and clinicians are gathered and provided., The multiple pathological effects of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, and its total novelty, mean that currently a lot of diagnostic and therapeutic tools, established and tentative alike, are needed to treat patients in a timely, effective way. In order to make these tools more reliable, faster and more feasible, biological fluid microsampling techniques could provide many advantages. In this review, the most important microsampling techniques are considered (dried matrix spots, volumetric absorptive microsampling, microfluidics and capillary microsampling, solid phase microextraction) and their respective advantages and disadvantages laid out. Moreover, currently available microsampling applications of interest for SARS-CoV-2 therapy are described, in order to make them as much widely known as possible, hopefully providing useful information to researchers and clinicians alike.

The current switching ripple in a three-phase four-wire split-capacitor converter is analyzed in this paper for all the four ac output wires in relation to both balanced and unbalanced working conditions. Specifically, analytical formulations of the peak-to-peak and root mean square (RMS) current ripples are originally evaluated as a function of the modulation index, separately for the three phases and the neutral wire. Initially, the single-carrier sinusoidal pulse width modulation (PWM) technique is outlined, as it generally concerns a straightforward and effective modulation. With the aim of mitigating the current ripple in the neutral wire, the interleaved multiple-carrier PWM strategy is adopted, also avoiding any repercussion on the phase one. Numerical simulations and experimental tests were carried out to verify all the analytical developments.

Three-phase, four-wire split capacitor inverters are currently employed in many applications, such as photovoltaic systems, battery chargers for electric vehicles, active power filters, and, in general, in all grid-tied applications that deal with possible grid voltage and/or current unbalances. This paper provides a comprehensive evaluation of the capacitor-switching voltage ripple and dc-link switching voltage ripple for the three-phase, four-wire, split capacitor inverters. Specifically, analytical formulations of the peak-to-peak and rms values of the voltage ripples are originally pointed out in this paper and determined in the case of balanced three-phase and unbalanced (two-phase and single-phase) output (ac) currents. The obtained results can help in designing the considered inverter and sizing of the dc-link capacitors. Reference is made to the sinusoidal PWM modulation and sinusoidal three-phase output currents with an almost unity power factor, representing a grid-connected application. Extensive numerical simulations have been carried out to thoroughly verify all the analytical developments presented in this paper. Furthermore, some experimental tests, having balanced output currents on the ac side, have been accomplished, validating numerical simulations and analytical developments.

In the context of electric vehicle (EV) development and positive energy districts with the growing penetration of non-programmable sources, this paper provides a method to predict and manage the aggregate power flows of charging stations to optimize the self-consumption and load profiles. The prediction method analyzes each charging event belonging to the EV population, and it considers the main factors that influence a charging process, namely the EV’s characteristics, charging ratings, and driver behavior. EV’s characteristics and charging ratings are obtained from the EV model’s and charging stations’ specifications, respectively. The statistical analysis of driver behavior is performed to calculate the daily consumptions and the charging energy request. Then, a model to estimate the parking time of each vehicle is extrapolated from the real collected data of the arrival and departure times in parking lots. A case study was carried out to evaluate the proposed method. This consisted of an industrial area with renewable sources and electrical loads. The obtained results show how EV charging can negatively impact system power flows, causing load peaks and high energy demand. Therefore, a charging management system (CMS) able to operate in the smart charging mode was introduced. Finally, it was demonstrated that the proposed method provides better EV integration and improved performance.

Amongst the processing technologies able to improve the functional features of cereal-based foods, sourdough fermentation using Lactic Acid Bacteria (LAB) has been recently rediscovered for its beneficial effects. Wheat (Triticum aestivum L.) bread doughs were prepared using LAB strains belonging to different Lactobacillus species and changes in phenolic acid, carotenoid content and antioxidant capacity were evaluated. Two L. plantarum strains out of six were able to significantly increase carotenoid content in the dough, suggesting that a higher mobilization/solubilisation of these antioxidant compounds occurs. Within different fractions (free, soluble-conjugated, insoluble-bound), the relative distribution of ferulic acid and antioxidant activity changes depending on the specific strain. Overall, results indicate that some LAB strains cause in situ changes, significantly increasing the content of functional compounds in doughs during fermentation. This, in turn, could improve the functional features of bakery foods characterised by a high content in carotenoids and other bioactive compounds.

Glycyrrhizin is the main active compound of Glycyrrhiza glabra root extracts; according to recent studies, glycyrrhizin and its aglycon, glycyrrhetic acid, have interesting therapeutic properties. A new capillary electrophoretic method has been developed for the separation and quantification of glycyrrhizin, beta-glycyrrhetic acid and its isomer a-glycyrrhetic acid. Separation of the analytes was achieved in less than 3 min on a fused silica capillary, by injecting the samples at the short end of the capillary (effective length: 8.5 cm). The background electrolyte was composed of pH 10.0 carbonate buffer, methanol and ethylene glycol (80/10/10) and contained 0.4% beta-cyclodextrin; indomethacin was used as the internal standard. Diode array detection was used, with quantitative assays carried out at 254 nm. Linearity was found over the 5-200 and 2.5-100 microg mL(-1) concentration ranges for glycyrrhizin and glycyrrhetic acid, respectively. This method has been applied to the determination of the analytes in different matrices (liquorice roots and commercial confectionery products), and to the purity control of beta-glycyrrhetic acid obtained from the hydrolysis of glycyrrhizin. When analysing beta-glycyrrhetic acid and its epimer in roots, the samples were purified by means of a suitable solid-phase extraction (SPE) procedure with Oasis HLB cartridges, which granted good selectivity, eliminating matrix interference.