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In Vitro Anti-Inflammatory Effect of Salvia sagittata Ethanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells.
- Source :
-
Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2019 May 09; Vol. 2019, pp. 6829173. Date of Electronic Publication: 2019 May 09 (Print Publication: 2019). - Publication Year :
- 2019
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Abstract
- The aim of the present research was to study the effects of an ethanolic extract of Salvia sagittata Ruiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs). pAECs were cultured in the presence of different concentrations (1-200 μ g/mL) of SSEE for 24 h, and cytotoxicity was evaluated by the MTT assay. SSEE did not negatively affect cellular viability at any concentration tested. Cell cycle was analyzed and no significant change was observed. Then, the anti-inflammatory effects of SSEE on pAECs were analyzed using a lipopolysaccharide (LPS) as the inflammatory stimulus. Different markers involved in the inflammatory process, such as cytokines and protective molecules, were evaluated by real-time quantitative PCR and Western blot. SSEE showed the ability to restore pAEC physiological conditions reducing interleukin-6 and increasing Heme Oxygenase-1 protein levels. The phytochemical composition of SSEE was also evaluated via HPLC-DAD and spectrophotometric assays. The presence of different phenolic acids and flavonoids was revealed, with rosmarinic acid as the most abundant component. SSEE possesses an interesting antioxidant activity, as assessed through both the Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. In conclusion, results suggest that SSEE is endowed with an in vitro anti-inflammatory effect. This represents the initial step in finding a possible scientific support for the traditional therapeutic use of this plant.
- Subjects :
- Animals
Anti-Inflammatory Agents chemistry
Aorta pathology
Camphanes
Drugs, Chinese Herbal chemistry
Endothelial Cells pathology
Ethanol chemistry
Heme Oxygenase-1 metabolism
Inflammation chemically induced
Inflammation drug therapy
Inflammation metabolism
Inflammation pathology
Lipopolysaccharides toxicity
Panax notoginseng
Primary Cell Culture
Salvia miltiorrhiza
Swine
Anti-Inflammatory Agents pharmacology
Aorta metabolism
Drugs, Chinese Herbal pharmacology
Endothelial Cells metabolism
Salvia chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1942-0994
- Volume :
- 2019
- Database :
- MEDLINE
- Journal :
- Oxidative medicine and cellular longevity
- Publication Type :
- Academic Journal
- Accession number :
- 31210845
- Full Text :
- https://doi.org/10.1155/2019/6829173