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Dried Urine Microsampling Coupled to Liquid Chromatography—Tandem Mass Spectrometry (LC–MS/MS) for the Analysis of Unconjugated Anabolic Androgenic Steroids
- Source :
- Molecules, Vol 25, Iss 3210, p 3210 (2020), Molecules, Volume 25, Issue 14
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Testing and monitoring anabolic androgenic steroids in biological fluids is a key activity in anti-doping practices. In this study, a novel approach is proposed, based on dried urine microsampling through two different workflows: dried urine spots (DUS) and volumetric absorptive microsampling (VAMS). Both techniques can overcome some common drawbacks of urine sampling, such as analyte instability and storage and transportation problems. Using an original, validated liquid chromatography&ndash<br />tandem mass spectrometry (LC-MS/MS) method, exogenous and endogenous unconjugated steroids were analysed. Despite the limitations of microsampling volume, good sensitivity was obtained (limit of quantitation &le<br />1.5 ng/mL for all analytes), with satisfactory precision (relative standard deviation &lt<br />7.6%) and absolute recovery (&gt<br />70.3%). Both microsampling platforms provide reliable results, in good agreement with those obtained from urine.
- Subjects :
- Analyte
Relative standard deviation
Pharmaceutical Science
Urine
anabolic androgenic steroids
Urinalysis
Mass spectrometry
Sensitivity and Specificity
01 natural sciences
Article
Analytical Chemistry
lcsh:QD241-441
03 medical and health sciences
0302 clinical medicine
lcsh:Organic chemistry
Tandem Mass Spectrometry
Liquid chromatography–mass spectrometry
Drug Discovery
Lc ms ms
Humans
Physical and Theoretical Chemistry
LC-MS/MS
Testosterone Congeners
dried urine spots (DUS)
Doping in Sports
Detection limit
Chromatography
Molecular Structure
Chemistry
010401 analytical chemistry
Organic Chemistry
anti-doping
Reproducibility of Results
Anabolic-Androgenic Steroids
0104 chemical sciences
Chemistry (miscellaneous)
microsampling
Molecular Medicine
Volumetric absorptive microsampling (VAMS)
neuroprotection
Anabolic androgenic steroid
030217 neurology & neurosurgery
Chromatography, Liquid
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 25
- Issue :
- 3210
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....d223fb291a47da1e1684888bfb7a8480