12 results on '"Liu, R. F."'
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2. Hydrothermal-electrochemical codeposited hydoxyapatite/yttria-stabilized zirconia composite coating
- Author
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Xiao, X. F., Liu, R. F., and Zheng, Y. Z.
- Published
- 2006
- Full Text
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3. FLGC-Fusion GAN: An Enhanced Fusion GAN Model by Importing Fully Learnable Group Convolution
- Author
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Yuan, C., primary, Sun, C. Q., additional, Tang, X. Y., additional, and Liu, R. F., additional
- Published
- 2020
- Full Text
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4. Expression and activity analysis reveal that heme oxygenase (decycling) I is associated with blue egg formation.
- Author
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Wang, Z. P., Liu, R. F., Wang, A. R., Li, J. Y., and Deng, X. M.
- Subjects
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HEME oxygenase , *EGGS , *GENE expression , *EGGSHELLS , *MESSENGER RNA - Abstract
Biliverdin is responsible for the coloration of blue eggs and is secreted onto the eggshell by the shell gland. Previous studies confirmed that a significant difference exists in biliverdin content between blue eggs and brown eggs, although the reasons are still unknown. Because the pigment is derived from oxidative degradation of heme catalyzed by heme oxygenase (HO), this study compared heme oxygenase (decycling) 1 (HMOX1), the gene encoding HO expression and HO activity, in the shell glands of the Dongxiang blueshelled chicken (n = 12) and the Dongxiang brownshelled chicken (n = 12). Results showed that HMOX1 was highly expressed at the mRNA (1.58-fold; P < 0.05) and protein levels in blue-shelled chickens compared with brown-shelled chickens. At the functional level, blue-shelled chickens also showed 1.40-fold (P < 0.05) higher HO activity than brown-shelled chickens. To explore the reasons for the differential expression of HMOX1, an association study of 6 SNP capturing the majority of HMOX1 variants with the blue egg coloration was performed. Results showed no significant association between SNP and the blue egg coloration in HMOX1 (P > 0.05). Taken together, these results show that blue egg formation is associated with high expression of HMOX1 in the shell gland of Dongxiang blue-shelled chickens, and suggest that differential expression of HMOX1 in the 2 groups of chickens is most likely to arise from an alteration in the trans-acting factor. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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5. Effects of lysine deficiency or excess on growth and the expression of lipid metabolism genes in slow-growing broilers.
- Author
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Tian, D L, Guo, R J, Li, Y M, Chen, P P, Zi, B B, Wang, J J, Liu, R F, Min, Y N, Wang, Z P, Niu, Z Y, and Liu, F Z
- Subjects
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CHOLESTERYL ester transfer protein , *LIPID metabolism , *LIPID synthesis , *ACETYL-CoA carboxylase , *ABDOMINAL adipose tissue , *CARRIER proteins - Abstract
This experiment was conducted to evaluate the effects of lysine deficiency or excess on growth and the expression of lipid metabolism genes in slow-growing birds. A total of 360 one-day-old chicks were randomly divided into 3 groups, with 6 replicates of 20 birds each. The birds fed the basal diet with a total lysine 0.60% (LL), 1.00% (ML), or 1.40% (HL). The amount of lysine (ML) as the control group, LL and HL as the experimental group, the trial period last 3 wk. The results showed that compared with ML, LL significantly decreased average daily gain and average daily feed intake and remarkably increased feed conversion ratio of birds at 21 day old (P < 0.01), while the above indices in HL had no significant effects (P > 0.05). Besides, LL reduced the pectoral muscle rate (P < 0.01) and decreased the percentage of abdominal fat significantly (P < 0.05). In addition, compared with ML, the expression of fatty acid binding protein 1 (FABP1), acetyl-CoA carboxylase (ACC), malic enzyme (ME), and sterol regulatory element binding protein 1 (SREBP1c) mRNA of liver in LL was significantly decreased (P < 0.05), and the expression of cholesteryl ester transfer protein (CETP) mRNA was significantly increased (P < 0.01), whereas LL had no significant effects on the expression of peroxisome proliferator activated receptor alpha (PPARα) mRNA (P > 0.05). Moreover, compared with ML, HL significantly reduced the expression of FABP1, ACC, ME, SREBP-1c , and PPARα mRNA in the liver (P < 0.05), and had no significant effects on the expression of CETP mRNA (P > 0.05). The results of current research suggest that dietary lysine deficiency could reduce the growth and fat deposition of slow-growing broilers mainly by downregulating the expression of lipid synthesis genes. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
6. [A single-center study on the oncological outcomes of active surveillance of thyroid nodules measuring≤1 cm with highly suspicious ultrasound features].
- Author
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Liu CH, Zhao H, Xia Y, Cao Y, Zhang LY, Zhao Y, Gao LY, Liu RF, Liu YW, Liu HF, Meng ZL, Liu SZ, Lu Y, Palashate Y, and Li XY
- Subjects
- Male, Female, Humans, Adult, Middle Aged, Cohort Studies, Prospective Studies, Watchful Waiting, Ultrasonography methods, Retrospective Studies, Disease Progression, Thyroid Nodule diagnostic imaging, Thyroid Nodule pathology, Thyroid Neoplasms
- Abstract
Objective: To investigate the oncological outcomes of active surveillance (AS) in patients showing thyroid nodules measuring≤1 cm with highly suspicious ultrasound features. Methods: A prospective single-center cohort study. A total of 534 patients with highly suspicious thyroid nodules (2015 American Thyroid Association Nodule Sonographic Patterns and Risk of Malignancy: High Suspicion) were enrolled in this study, the patients received AS at Peking Union Medical College Hospital between January 2017 and November 2022 to assess oncological outcomes (disease progression, recurrence/metastasis rate, etc). The patients were followed up every 6 months for physical examination and neck ultrasound examination. And the value of tumor volume changes in evaluating tumor enlargement was explored too. Results: There were 413 females and 121 males in this cohort, with a mean age of (42.6±11.8) years. During a median follow-up period of 45.6 months (ranged from 3.5 to 176.0 months), disease progression occurred in 26 patients (4.9%) with highly suspicious thyroid nodules, characterized by a minimum 3-mm increase in tumor diameter in 19 patients (3.6%) and lymph node metastases in 7 patients (1.3%). Forty-seven (8.8%) patients opted for delayed surgery, with 29 patients due to a change in preference. There was no significant differences in pathologic and follow-up outcomes between patients with disease progression and preference change. Patients aged≤40 years had a higher cumulative incidence of 5-year disease progression than those aged>40 years (4.9% vs 1.9%, P =0.060). No patients experienced distant metastases or deaths. Among the 595 high-risk thyroid nodules with continuous volume assessment results and an increase in nodule diameter of less than 3 mm (including all high-risk nodules in patients with single or multiple nodules), 184 (30.9%) and 79 (13.3%) nodules exhibited volume increases of more than 50% and 100%, respectively, in multiple measurements. Among the nodules with volume changes exceeding 50% and 100%, the proportion of nodules with a baseline tumor diameter of≤0.5 cm was significantly higher than those with a diameter of>0.5 cm, at 69.0% vs 31.0% ( P <0.001) and 77.2% vs 22.8% ( P <0.001), respectively . Conclusions: Active surveillance in patients with highly suspicious subcentimeter thyroid nodules has good short-term oncological outcomes and can be considered a safe alternative to surgery. Due to the large variability in the measurement results of tumor volume, it is not suitable as an indicator for evaluating tumor enlargement .
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- 2023
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7. Increased miR-155-5p expression in dermal mesenchymal stem cells of psoriatic patients: comparing the microRNA expression profile by microarray.
- Author
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Hou RX, Liu RF, Zhao XC, Jia YR, An P, Hao ZP, Li JQ, Li XH, Yin GH, and Zhang KM
- Subjects
- Adaptor Proteins, Signal Transducing metabolism, Adult, Case-Control Studies, Dermis pathology, Female, Humans, Male, Mesenchymal Stem Cells pathology, MicroRNAs metabolism, Middle Aged, Nitric Oxide Synthase Type II genetics, Nitric Oxide Synthase Type II metabolism, Oligonucleotide Array Sequence Analysis, Psoriasis metabolism, Psoriasis pathology, Real-Time Polymerase Chain Reaction, Severity of Illness Index, Signal Transduction, Adaptor Proteins, Signal Transducing genetics, Dermis metabolism, Gene Expression Regulation, Mesenchymal Stem Cells metabolism, MicroRNAs genetics, Psoriasis genetics
- Abstract
Mesenchymal stem cells (MSCs) have pleiotropic immuno-modulatory effects and pro-angiogenic ability, leading to the presumption that MSCs may be involved in the pathogenesis of many inflammatory or autoimmune disorders, including psoriasis. In a previous study, we reported the specific gene expression profile of dermal MSCs from psoriasis. Inflammation- and angiogenesis-related genes, such as lipopolysaccharide-induced tumor necrosis factor-alpha transcription factor (LITAF), dual-specificity protein phosphatase 1 (DUSP1), vascular endothelial growth factor α (VEGFα), and insulin-like growth factor-binding protein-5 (IGFBP5), are abnormally expressed in psoriatic dermal MSCs. As a key regulator of gene expression, miRNA are involved in a wide variety of biological processes; in fact, several miRNAs have been implicated in the development and progression of inflammatory or autoimmune disorders. In this study, we compared the miRNA expression profiles of dermal MSCs from patients with psoriasis to those in MSCs from normal individuals by microarray, and found that the pro-inflammatory miRNA miR-155 was significantly overexpressed in psoriatic MSCs (2.44 fold, P < 0.001). Additionally, the expression of miR-155 target gene TAB2 (8.47 ± 1.55 vs 6.38 ± 2.10, P < 0.01,) and the downstream gene iNOS (5.26 ± 2.58 vs 3.73 ± 1.89, P < 0.05) was found to be inhibited in psoriatic dermal MSCs by real-time PCR. Therefore, we speculated that the elevation in miR-155 levels may be an indicator of, or a key regulatory pathway in, the pathogenesis of psoriasis, resulting in functionally impaired dermal MSCs.
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- 2016
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8. Research Note Mesenchymal stem cells from skin lesions of psoriasis patients promote proliferation and inhibit apoptosis of HaCaT cells.
- Author
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Liu RF, Wang F, Wang Q, Zhao XC, and Zhang KM
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- Adult, Antigens, CD biosynthesis, Antigens, CD genetics, Apoptosis genetics, Cell Differentiation genetics, Cell Proliferation genetics, Epidermis metabolism, Epidermis pathology, Female, Flow Cytometry, Humans, Keratinocytes metabolism, Male, Mesenchymal Stem Cells metabolism, Middle Aged, Psoriasis pathology, Skin metabolism, Keratinocytes pathology, Mesenchymal Stem Cells pathology, Psoriasis genetics, Skin pathology
- Abstract
Psoriasis is an inflammatory skin disease characterized by excessive proliferation and abnormal differentiation and apoptosis of keratinocytes (KCs). Mesenchymal stem cells (MSCs) from skin lesions of psoriasis patients demonstrate abnormal cytokine secretion, which may affect KC proliferation and apoptosis. Here, we explored how MSCs from skin lesions of psoriasis patients affect HaCaT cell proliferation and apoptosis. First, flow cytometry and multipotent differentiation methods were used to identify skin MSCs, which were then co-cultured with HaCaT cells. HaCaT cell proliferation was analyzed in real-time, and cell cycle progression and apoptosis were assessed by flow cytometry. Cell morphologies and multipotencies of skin MSCs were similar between the psoriasis group and healthy control group, with high levels of CD29, CD44, CD73, CD90, and CD105 and limited expression of CD34, CD45, and HLA-DR. MSCs from skin lesions of psoriasis patients promote KC proliferation more potently and are less capable of inducing KC apoptosis. This may underlie KC proliferation and abnormal apoptosis in psoriasis skin lesions, which results in abnormal thickening of the epidermis.
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- 2015
- Full Text
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9. LITAF, HHEX, and DUSP1 expression in mesenchymal stem cells from patients with psoriasis.
- Author
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Chang WJ, Niu XP, Hou RX, Li JQ, Liu RF, Wang Q, Wang CF, Li XH, Yin GH, and Zhang KM
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- Adult, Case-Control Studies, Dual Specificity Phosphatase 1 metabolism, Female, Homeodomain Proteins metabolism, Humans, Male, Middle Aged, Nuclear Proteins metabolism, Psoriasis diagnosis, RNA, Messenger genetics, RNA, Messenger metabolism, Severity of Illness Index, Transcription Factors metabolism, Young Adult, Dual Specificity Phosphatase 1 genetics, Gene Expression, Homeodomain Proteins genetics, Mesenchymal Stem Cells metabolism, Nuclear Proteins genetics, Psoriasis genetics, Transcription Factors genetics
- Abstract
Psoriasis is a common chronic relapsing inflammatory skin disease, in which mesenchymal stem cells (MSCs) have been hypothesized to play an important role in abnormal localized inflammation and vascular proliferation observed in skin lesions. Previous studies have revealed abnormal gene expression patterns, DNA methylation status, and cytokine secretion of MSCs in psoriatic skin lesions, as well as some gene expression abnormalities related to inflammation and angiogenesis. We further verified the gene and protein expressions of inflammation-related lipopolysaccharide-induced tumor necrosis factor-alpha transcription factor (LITAF), dual-specificity protein phosphatase 1 (DUSP1), and angiogenesis-related hematopoietically expressed homeobox (HHEX) in MSCs derived from the skin lesions of psoriasis patients. The gene expression of LITAF, DUSP1, and HHEX in dermal MSCs was measured at the mRNA level using reverse transcription-polymerase chain reaction and the corresponding protein expression levels were analyzed by western blotting analysis. The gene and protein expression levels of LITAF, HHEX, and DUSP1 in dermal MSCs were significantly lower in psoriasis patients compared to controls. Amplification and western blotting results were consistent with our previously reported gene chip data. Our results suggest that dermal MSCs in psoriatic skin lesions may be involved in the development, progression, and regulation of localized inflammatory abnormalities by reducing the expression of LITAF, HHEX, and DUSP1, which are related to inflammation and angiogenesis.
- Published
- 2015
- Full Text
- View/download PDF
10. PRESBYOPIA OPTOMETRY METHOD BASED ON DIOPTER REGULATION AND CHARGE COUPLE DEVICE IMAGING TECHNOLOGY.
- Author
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Zhao Q, Wu XX, Zhou J, Wang X, Liu RF, and Gao J
- Subjects
- Humans, Diagnostic Imaging instrumentation, Diagnostic Imaging methods, Eye pathology, Eye physiopathology, Microcomputers, Optometry instrumentation, Optometry methods, Presbyopia diagnosis, Presbyopia physiopathology
- Abstract
With the development of photoelectric technology and single-chip microcomputer technology, objective optometry, also known as automatic optometry, is becoming precise. This paper proposed a presbyopia optometry method based on diopter regulation and Charge Couple Device (CCD) imaging technology and, in the meantime, designed a light path that could measure the system. This method projects a test figure to the eye ground and then the reflected image from the eye ground is detected by CCD. The image is then automatically identified by computer and the far point and near point diopters are determined to calculate lens parameter. This is a fully automatic objective optometry method which eliminates subjective factors of the tested subject. Furthermore, it can acquire the lens parameter of presbyopia accurately and quickly and can be used to measure the lens parameter of hyperopia, myopia and astigmatism.
- Published
- 2015
11. Salt stress represses production of extracellular proteases in Bacillus pumilus.
- Author
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Liu RF, Huang CL, and Feng H
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- Bacillus drug effects, Endopeptidases genetics, Fermentation, Gene Expression Regulation, Bacterial drug effects, Sodium Chloride pharmacology, Bacillus enzymology, Endopeptidases biosynthesis, Stress, Physiological drug effects
- Abstract
Bacillus pumilus is able to secrete subtilisin-like prote-ases, one of which has been purified and characterized biochemically, demonstrating great potential for use in industrial applications. In the current study, the biosynthesis and transcription of extracellular pro-teases in B. pumilus (BA06) under salt stress were investigated using various methods, including a proteolytic assay, zymogram analysis, and real-time PCR. Our results showed that total extracellular proteolytic activity, both in fermentation broth and on milk-containing agar plates, was considerably repressed by salt in a dosage-dependent manner. As Bacillus species usually secret multiple extracellular proteases, a vari-ety of individual extracellular protease encoding genes were selected for real-time PCR analysis. It was shown that proteases encoded by the aprE and aprX genes were the major proteases in the fermentation broth in terms of their transcripts in B. pumilus. Further, transcription of aprE, aprX, and epr genes was indeed repressed by salt stress. In con-trast, transcription of other genes (e.g., vpr and wprA) was not repressed or significantly affected by the salt. Conclusively, salt stress represses total extracellular proteolytic activity in B. pumilus, which can largely be ascribed to suppression of the major protease-encoding genes (aprE, aprX) at the transcriptional level. In contrast, transcription of other pro-tease-encoding genes (e.g., vpr, wprA) was not repressed by salt stress.
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- 2015
- Full Text
- View/download PDF
12. Impact of BMMSCs from different sources on proliferation of CD34⁺ cells.
- Author
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Liu RF, Li JQ, Hou RX, Wang R, and Zhang KM
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- Adult, Cell Proliferation, Cell Separation, Cells, Cultured, Female, Flow Cytometry, Humans, Male, Middle Aged, Psoriasis pathology, Young Adult, Antigens, CD34 metabolism, Bone Marrow Cells cytology, Mesenchymal Stem Cells cytology
- Abstract
There are significant differences on the biological characteristics of bone marrow mesenchymal stem cells (BMMSCs), immunological response, and antigen-presenting functions between patients with psoriasis and normal subjects, but there are no significant differences in aborted fetuses. We examined the differences in BMMSCs between aborted fetuses and patients with psoriasis in this study. Bone marrow from normal subjects, aborted fetuses, and patients with psoriasis were obtained using a MidiMACS machine. Density gradient centrifugation method was used to isolate the bone marrow mononuclear cells of patients with psoriasis and aborted fetus and the cells were cultivated. Bone marrow CD34(+) cells from normal subjects were isolated. MTT colorimetric detection was used to test the proliferation activity of bone marrow CD34(+) cells. The purity of bone marrow CD34(+) cells and BMMSCs was determined by flow cytometry. The BMMSC culture supernatant fluid of patients with psoriasis and aborted fetuses showed no statistically significant difference with bone marrow CD34(+) cell proliferation in normal subjects (P > 0.05).
- Published
- 2015
- Full Text
- View/download PDF
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