201. Immunochromatographic paper sensor for ultrasensitive colorimetric detection of cadmium.
- Author
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Song, Shanshan, Zou, Shuzhen, Zhu, Jianping, Liu, Liqiang, and Kuang, Hua
- Subjects
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COLORIMETRY , *CADMIUM , *CHROMATOGRAPHIC analysis , *MONOCLONAL antibody probes , *ETHYLENEDIAMINETETRAACETIC acid - Abstract
A novel and highly sensitive immunochromatographic strip based on a monoclonal antibody (3A9) was developed for the detection of cadmium in tap water. The 50% inhibition concentration of the antibody, which showed no cross-reactivity with other heavy metal ions, was 0.45 ng/mL and it recognized Cd(II)-1-(4-isothiocyanobenzyl) ethylenediamine-N,N,N′,N′-tetraacetic acid (EDTA) (Cd(II)-ITCBE) and not metal-free ITCBE and EDTA. The cutoff value for semi-quantitative detection of the strip was 5 ng/mL and the lower limit of detection for quantitative detection was 0.2 ng/mL using a scanning reader. The percent recovery ranged from 107.6% to 132% in tap water samples. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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