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This article, published in Histochemistry & Cell Biology, focuses on two methodological studies. The first study investigates the effect of oxygen levels on oviduct air-liquid interface epithelial cell culture models. The second study examines the possibility of detecting RNA in paraffin sections from mouse brain with delayed fixation. The results of the first study show that oxygen tension and media affect cell populations and polarization, while the second study finds that postmortem processing delay can impact the detection of RNA in brain tissue. The authors emphasize the importance of maintaining a physiologically relevant environment for cultured cells and suggest that further technological improvements may allow for the detection of RNA in brain tissues processed later than 48 hours postmortem. [Extracted from the article]

This article from Histochemistry & Cell Biology highlights three studies. The first study examines the response of fibroblast cultures from different tissues to treatment with TGF-β1 and TGF-β3, finding that fibroblasts from different sources have heterogeneous phenotypes and activated signaling pathways. The second study investigates the presence of gonadotropin-releasing hormone (GnRH)1 mRNA in late-evolving salmonids, finding that only two forms of GnRH are known in these species. The third study provides a comprehensive review of non-canonical DNA structures in human ribosomal RNA. The article also mentions new guidelines for publishing images and image analyses. [Extracted from the article]

The journal Histochemistry & Cell Biology has implemented new submission guidelines for image presentation and analysis. These guidelines were developed by the "Image Analysis and Visualization" workgroup of the international organization Quality Assessment and Reproducibility for Instruments & Images in Light Microscopy (QUAREP-LiMi). The guidelines consist of checklists that provide "minimal," "recommended," and "ideal" levels for image format, colors, annotation, and availability, as well as image analysis protocols. The goal of these guidelines is to enhance the rigor and reproducibility of published microscopy images and analyses. Histochemistry & Cell Biology, as a Springer Nature journal, is implementing these checklists to ensure the highest levels of rigor and reproducibility in the images and analyses they publish. [Extracted from the article]

At the occasion of the 65th anniversary of Histochemistry and Cell Biology, we browse through its first ten years of publication and highlight a selection of papers from the early days of enzyme, protein, and carbohydrate histochemistry. In addition, we narrate recent progress to identify, quantify, and precisely determine the tissue localization of proteins and lipids, and small molecules by the combination of spectroscopic techniques and histology. [ABSTRACT FROM AUTHOR]

Copyright of JMD: Journal für Mathematik-Didaktik is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of JMD: Journal für Mathematik-Didaktik is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Autophagy is a degradative cellular process that can be both non-selective and selective and begins with the formation of a unique smooth double-membrane phagophore which wraps around a portion of the cytoplasm. Excess and damaged organelles and cytoplasmic protein aggregates are degraded by selective autophagy. Previously, we reported that in fed HepG2 cells, cytoplasmic aggregates of EDEM1 and surplus fibrinogen Aα–γ assembly intermediates are targets of selective autophagy receptors and become degraded by a selective autophagy called aggrephagy. Here, we show by multiple confocal immunofluorescence and colocalization panels the codistribution of cytoplasmic protein aggregates with the selective autophagy receptors p62/SQSTM1 and NBR1 and with the phagophore marker LC3, and that phagophores induced by vinblastine treatment contain complexes of protein aggregates and selective autophagy receptors. By combined serial ultrathin section analysis and immunoelectron microscopy, we found that in fed HepG2 cells, a basically ribosome-free subdomain of rough endoplasmic reticulum (RER) cisternae forms a cradle that engulfs the cytoplasmic protein aggregates. This RER subdomain appears structurally different from omegasomes formed by the RER, which were suggested to provide a membrane platform from which the phagophore is derived in starvation-induced autophagy. Taken together, our observations provide further evidence for the importance of RER subdomains as a site and membrane source for phagophore formation and show their involvement in selective autophagy. [ABSTRACT FROM AUTHOR]

The article discusses the implementation of guidelines for the use of large language models (LLMs), specifically focusing on the AI chatbot ChatGPT developed by OpenAI. The guidelines state that LLMs cannot be credited as authors on research papers due to the lack of accountability. However, authors using LLMs must disclose their use in the manuscript. These principles have been adopted by Histochemistry and Cell Biology, a journal published by Springer Nature. The article emphasizes the importance of ethical use of LLMs in research. [Extracted from the article]

Copyright of JMD: Journal für Mathematik-Didaktik is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Highlights from the article: Graph: Fig. 1The collage shows the cover pictures of the 12 issues of Histochemistry and Cell Biology published on the occasion of the journal's 50th anniversary in 2008. Moreover, since 2004, five eminent scientists have been selected to present the I Histochemistry and Cell Biology i Lecture at either the Annual Symposium of the Society for Histochemistry, or at the International Congress of Histochemistry and Cytochemistry (Table 3), with their presentations likewise published by the journal. In 1995, concomitant with its change of name to the current I Histochemistry and Cell Biology i , the journal announced publishing policies stipulating that (1) manuscripts will be published in full format without arbitrary page limits, and (2) no charge for the first color plate (Drenckhahn and Roth [15]). 47 Roth J, Fahimi HD, Drenckhahn D. Celebration of the 50th anniversary of publication of Histochemistry and Cell Biology.

Copyright of JMD: Journal für Mathematik-Didaktik is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Carbohydrates have physiological importance far beyond their roles as source of energy (glycolysis) and activated hydrogen for synthesis (pentosephosphate pathway) or as constituent of the backbone of nucleic acids and of cell wall polysaccharides. The extent of compositional and structural variability of their oligomers (glycans) is unsurpassed in Nature due to the unique property of independently combining the following parameters with sequence: anomeric status, linkage positions, ring size, addition of branches and site-specific introduction of substitutions. The monosaccharides (letters of the third alphabet of life) thus generate 'words' (signals) of high-density coding capacity. These 'words' are part of the glycans on proteins and lipids, and the glycome represented by these 'words' in their entirety has cell type-dependent features. The often limited intramolecular flexibility of oligosaccharides along with an abundance of contact points for intermolecular interactions is ideal for binding processes. Glycan-based 'words' can thus be 'read,' and their message translated into cellular effects by receptors called lectins. This journal's special issue covers central aspects of the concept of the sugar code. [ABSTRACT FROM AUTHOR]

Protein N-glycosylation and quality control of protein folding as well as the connected ER-associated degradation of misfolded glycoproteins (ERAD) are not only evolutionary highly conserved but also functionally linked. It is now established that particular N-glycan structures which result from processing reactions by exo-glycosidases in the ER are of importance for glycoprotein folding and for ERAD. Thus, mono-glucosylated N-glycan intermediates harbor structural information which is important for promoting glycoprotein folding. On the other hand, specific mannose-trimmed N-glycans harbor structural information for routing misfolded glycoproteins to ERAD. In this review, we summarize current knowledge concerning the role played by glucosidases I and II, in concert with the bifunctional glucosyltransferase and calnexin/calreticulin in glycoprotein folding, the role of conventional ER mannosidase I in concert with the mannosidase EDEM1 in handling and routing of misfolded glycoproteins, and how the bifunctional OS-9 provides a link to the ER dislocon for degradation. [ABSTRACT FROM AUTHOR]