In focus in HCB.

This article, published in Histochemistry & Cell Biology, focuses on two methodological studies. The first study investigates the effect of oxygen levels on oviduct air-liquid interface epithelial cell culture models. The second study examines the possibility of detecting RNA in paraffin sections from mouse brain with delayed fixation. The results of the first study show that oxygen tension and media affect cell populations and polarization, while the second study finds that postmortem processing delay can impact the detection of RNA in brain tissue. The authors emphasize the importance of maintaining a physiologically relevant environment for cultured cells and suggest that further technological improvements may allow for the detection of RNA in brain tissues processed later than 48 hours postmortem. [Extracted from the article]