1. Secreted recombinant human IL-24 protein inhibits the proliferation of esophageal squamous cell carcinoma Eca-109 cells in vitro and in vivo
- Author
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Xuefeng Deng, Heyu Song, Chi Zhang, Qunfeng Ma, Pengkun Wang, Dan Luo, Bangming Jin, Hong Jiang, Xue Li, Zhinan Chen, Ziling Wang, Yinan Shi, Yan Liu, Yao Zhang, and Cuimi Duan
- Subjects
STAT3 Transcription Factor ,0301 basic medicine ,Cancer Research ,Esophageal Neoplasms ,medicine.medical_treatment ,Cell ,Mice, Nude ,Antineoplastic Agents ,Apoptosis ,CHO Cells ,Biology ,03 medical and health sciences ,chemistry.chemical_compound ,Cricetulus ,0302 clinical medicine ,In vivo ,Cell Line, Tumor ,Cricetinae ,medicine ,Animals ,Humans ,Cell Proliferation ,Mice, Inbred BALB C ,Cell growth ,Interleukins ,Receptors, Interleukin ,General Medicine ,Cell cycle ,Recombinant Proteins ,HEK293 Cells ,030104 developmental biology ,Cytokine ,medicine.anatomical_structure ,Oncology ,chemistry ,Cell culture ,030220 oncology & carcinogenesis ,Carcinoma, Squamous Cell ,Cancer research ,Female ,Esophageal Squamous Cell Carcinoma ,Growth inhibition ,A431 cells ,Neoplasm Transplantation ,Signal Transduction - Abstract
Interleukin-24 (IL-24) displays cancer-specific apoptosis-inducing properties in a broad spectrum of human tumors without harmful effects on normal cells. The human IL-24 protein is secreted as a glycosylated protein and functions as a pro-Th1 cytokine and a potent antiangiogenic molecule. However, the function of secreted recombinant human IL-24 (srhIL-24) protein in esophageal squamous cell carcinoma (ESCC) cells has not been studied. In the present study, we prepared a stable site-specific-integrated cell line, Flp-InTMCHO/IL-24 (FCHO/IL-24), which secreted rhIL-24 at a higher level than three random-integrated cell lines. In vitro, we identified that the purified srhIL-24 inhibited proliferation and induced the apoptosis of ESCC Eca-109 cells and activated STAT3, which was related with the IL-20 receptors. In vivo, the tumorigenicity of Eca-109 cells was significantly inhibited by s.c. injection of FCHO/IL-24 cells. Decreased tumor microvessel density and an increased number of TUNEL-positive tumor cells were associated with tumor growth inhibition, indicating the presence of antiangiogenic activity and induction of apoptotic activity. In summary, the present study demonstrated that srhIL-24 induced growth inhibition and apoptosis in ESCC Eca-109 cells in vitro and in vivo, which may be mediated by the receptor pathway.
- Published
- 2016