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Secreted recombinant human IL-24 protein inhibits the proliferation of esophageal squamous cell carcinoma Eca-109 cells in vitro and in vivo
- Source :
- Oncology Reports. 35:2681-2690
- Publication Year :
- 2016
- Publisher :
- Spandidos Publications, 2016.
-
Abstract
- Interleukin-24 (IL-24) displays cancer-specific apoptosis-inducing properties in a broad spectrum of human tumors without harmful effects on normal cells. The human IL-24 protein is secreted as a glycosylated protein and functions as a pro-Th1 cytokine and a potent antiangiogenic molecule. However, the function of secreted recombinant human IL-24 (srhIL-24) protein in esophageal squamous cell carcinoma (ESCC) cells has not been studied. In the present study, we prepared a stable site-specific-integrated cell line, Flp-InTMCHO/IL-24 (FCHO/IL-24), which secreted rhIL-24 at a higher level than three random-integrated cell lines. In vitro, we identified that the purified srhIL-24 inhibited proliferation and induced the apoptosis of ESCC Eca-109 cells and activated STAT3, which was related with the IL-20 receptors. In vivo, the tumorigenicity of Eca-109 cells was significantly inhibited by s.c. injection of FCHO/IL-24 cells. Decreased tumor microvessel density and an increased number of TUNEL-positive tumor cells were associated with tumor growth inhibition, indicating the presence of antiangiogenic activity and induction of apoptotic activity. In summary, the present study demonstrated that srhIL-24 induced growth inhibition and apoptosis in ESCC Eca-109 cells in vitro and in vivo, which may be mediated by the receptor pathway.
- Subjects :
- STAT3 Transcription Factor
0301 basic medicine
Cancer Research
Esophageal Neoplasms
medicine.medical_treatment
Cell
Mice, Nude
Antineoplastic Agents
Apoptosis
CHO Cells
Biology
03 medical and health sciences
chemistry.chemical_compound
Cricetulus
0302 clinical medicine
In vivo
Cell Line, Tumor
Cricetinae
medicine
Animals
Humans
Cell Proliferation
Mice, Inbred BALB C
Cell growth
Interleukins
Receptors, Interleukin
General Medicine
Cell cycle
Recombinant Proteins
HEK293 Cells
030104 developmental biology
Cytokine
medicine.anatomical_structure
Oncology
chemistry
Cell culture
030220 oncology & carcinogenesis
Carcinoma, Squamous Cell
Cancer research
Female
Esophageal Squamous Cell Carcinoma
Growth inhibition
A431 cells
Neoplasm Transplantation
Signal Transduction
Subjects
Details
- ISSN :
- 17912431 and 1021335X
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Oncology Reports
- Accession number :
- edsair.doi.dedup.....53b7e8277ae3a27b8066cd6cb32ee11b