Back to Search
Start Over
A novel human interleukin-24 peptide created by computer-guided design contributes to suppression of proliferation in esophageal squamous cell carcinoma Eca-109 cells
- Source :
- Oncology Reports. 33:193-200
- Publication Year :
- 2014
- Publisher :
- Spandidos Publications, 2014.
-
Abstract
- Based on the three-dimensional modeling structure of human interleukin-24 (hIL-24) and its most likely active position predicted by solvent accessibility and apparent electrostatic properties, a novel hIL-24 peptide M1 was created by computer-guided molecular design. The cytotoxicity and cell selectivity of M1 were examined in three human carcinoma cell lines and one normal human embryo lung fibroblast cell line (HEL). MTT assay showed that M1 induced growth arrest in two IL-20 receptor complex-positive cancer cell lines (the esophageal squamous cell carcinoma cell line Eca-109 and the melanoma cell line A375), and antibodies against IL-24 or IL-20 receptor complexes significantly neutralized the inhibitory activity. Moreover, M1 had almost no cytotoxicity on the lung cancer A549 cell line, which lacks a full complement of the IL-20 receptor complexes, or on HEL cells that express the IL-20 receptor complexes. These findings demonstrate that M1 could act as an excellent candidate for the induction of growth arrest on receptor complex-positive cancer cells. In summary, the M1 peptide may represent a novel anticancer agent for esophageal squamous cell carcinoma therapy due to its cancer cell selectivity and its relatively low cytotoxicity to normal cells.
- Subjects :
- Models, Molecular
Cancer Research
Pathology
medicine.medical_specialty
Esophageal Neoplasms
Cell Survival
Protein Conformation
Cell
Antineoplastic Agents
Biology
Inhibitory Concentration 50
Cell Line, Tumor
medicine
Humans
Cytotoxicity
Cell Proliferation
A549 cell
Interleukins
Cancer
General Medicine
Cell cycle
medicine.disease
medicine.anatomical_structure
Oncology
Cell culture
Drug Design
Cancer cell
Carcinoma, Squamous Cell
Cancer research
Computer-Aided Design
Esophageal Squamous Cell Carcinoma
A431 cells
Subjects
Details
- ISSN :
- 17912431 and 1021335X
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Oncology Reports
- Accession number :
- edsair.doi.dedup.....c409ec035be74f393b7c8f863f3610ab