1. Traffic light type paper-based analytical device for intuitive and semi-quantitative naked-eye signal readout
- Author
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Sera Ohta, Ryuya Hiraoka, Yuki Hiruta, and Daniel Citterio
- Subjects
Paper ,Point-of-Care Testing ,Lab-On-A-Chip Devices ,Biomedical Engineering ,Bioengineering ,Hydrogen Peroxide ,General Chemistry ,Microfluidic Analytical Techniques ,Biochemistry - Abstract
Microfluidic paper-based analytical devices (μPADs) have attracted great attention as potential candidates for point-of-care testing (POCT). Nevertheless, only a limited number of μPADs expected to satisfy the standard of Clinical Laboratory Improvement Amendments (CLIA) waived tests as issued by the US Food and Drug Administration (FDA) have been reported. This work introduces a "traffic light type μPAD", enabling highly intuitive semi-quantitative equipment-free naked-eye readout with no need for calibration, subjective interpretation or calculation. Assay results are displayed as traffic light colours reporting 5 analyte concentration levels (green/greenyellow/yellow/yellowred/red). The device has been designed to never display all three colours simultaneously, eliminating any risk for misinterpretation. The mechanism relies on the modulation of sample flow through a network of paperfluidic channels modified with a hydrophobic to hydrophilic phase-switching substance responsive to H
- Published
- 2022