Back to Search
Start Over
In situ paper-based 3D cell culture for rapid screening of the anti-melanogenic activity
- Source :
- The Analyst. 144:290-298
- Publication Year :
- 2019
- Publisher :
- Royal Society of Chemistry (RSC), 2019.
-
Abstract
- Recently, paper has gained traction in the biotechnology research field due to its ability to be a substrate for 3D cell culture. In this work, we demonstrate the application of paper-based 3D cell culture for rapid and easy screening of the effect of natural compounds on melanin production. Whatman No. 1 filter paper was used as the substrate for B16F10 melanoma cell culture. The use of paper is beneficial for supporting the 3D structure of cells, which makes the result more reliable due to the similarity to in vivo conditions. Furthermore, paper is beneficial for melanin observation due to melanin's black color, which is easily in situ visualized after it is cultured on white paper. Matrigel was used to encapsulate cells before being pipetted onto the paper to prevent the passing of cells through paper pores. The intensity of melanin can then be observed with the naked eye and analyzed by scanning the paper. The analysis process took only 20 minutes, which is faster than that of the conventional absorbance spectroscopy, owing to the elimination of centrifugation, melanin solubilization, and the absorbance measurement step. The color intensity on the paper showed a direct proportion with increased α-MSH concentrations, confirming that the color on the paper was melanin. The 3D structure of cells was confirmed by using a scanning electron microscope. To demonstrate the application of the paper-based scaffold, paper-based 3D cell culture was used for screening the effects of Kojic acid and Arbutin on melanin production, which showed increased anti-melanogenesis effects with increased concentrations of natural compounds. High cell viability was observed over 120 hours. In conclusion, the developed paper-based scaffold can be used for screening the effect of natural compounds on melanin production, as a rapid and simple method with low cost.
- Subjects :
- Paper
Cell Survival
Cell Culture Techniques
Drug Evaluation, Preclinical
02 engineering and technology
01 natural sciences
Biochemistry
Analytical Chemistry
Melanin
Absorbance
Mice
chemistry.chemical_compound
3D cell culture
Limit of Detection
Cell Line, Tumor
Electrochemistry
Animals
Environmental Chemistry
Spectroscopy
Melanins
Matrigel
Filter paper
010401 analytical chemistry
Arbutin
021001 nanoscience & nanotechnology
0104 chemical sciences
chemistry
Pyrones
alpha-MSH
Cell culture
Biophysics
0210 nano-technology
Kojic acid
Subjects
Details
- ISSN :
- 13645528 and 00032654
- Volume :
- 144
- Database :
- OpenAIRE
- Journal :
- The Analyst
- Accession number :
- edsair.doi.dedup.....674f62a6efdfc9caeb5bb082e860556c