1. The small molecule probe PT-Yellow labels the renal proximal tubules in zebrafish.
- Author
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Sander, Veronika, Patke, Shantanu, Sahu, Srikanta, Teoh, Chai Lean, Zhenzhen Peng, Young-Tae Chang, and Davidson, Alan J.
- Subjects
SMALL molecules ,PROXIMAL kidney tubules ,ZEBRA danio ,BLOOD filtration ,INTRAPERITONEAL injections ,FLUORESCENT probes - Abstract
We report the development of a small fluorescent molecule, BDNCA3-D2, herein referred to as PT-Yellow. Soaking zebrafish embryos in PT-Yellow or intraperitoneal injection into adults results in non-toxic in vivo fluorescent labeling of the renal proximal tubules, the major site of blood filtrate reabsorption and a common target of injury in acute kidney injury. We demonstrate the applicability of this new compound as a rapid and simple readout for zebrafish kidney filtration and proximal tubule reabsorption function. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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