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A fluorescent probe for imaging symmetric and asymmetric cell division in neurosphere formation.

Authors :
Seong-Wook Yun
Cheryl Leong
Yee Ling Tan
Xuezhi Bi
Young-Tae Chang
Hyung-Ho Ha
Jun-Young Kim
Hariharan, Srivats
Yuan Hong Yu
Narayanan, Gunaseelan
Sankaran, Shvetha
Ahmed, Sohail
Source :
Chemical Communications; 7/18/2014, Vol. 50 Issue 56, p7492-7494, 3p
Publication Year :
2014

Abstract

We report here a novel fluorescent chemical probe CDy5 which stains distinct neural stem/progenitor cells (NSPCs) by binding to acid ceramidase in mouse neurospheres. CDy5 is distributed evenly or unevenly to the daughter cells during multiple mitoses enabling the live imaging of symmetric and asymmetric divisions of isolated NSPCs. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
13597345
Volume :
50
Issue :
56
Database :
Complementary Index
Journal :
Chemical Communications
Publication Type :
Academic Journal
Accession number :
100182208
Full Text :
https://doi.org/10.1039/c4cc02974g