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Start Over Topic genetic variation Publisher microbiology research foundation
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1. Genetic and functional diversity of Bacillus strains in the soils long-term irrigated with paper and pulp mill effluent.

Author

Yadav S, Kaushik R, Saxena AK, and Arora DK

Subjects
Agricultural Irrigation standards, Bacillaceae isolation & purification, Bacillaceae metabolism, Bacillus classification, Bacillus genetics, Bacillus isolation & purification, Bacillus metabolism, Base Sequence, Cluster Analysis, Fresh Water, Hydrogen-Ion Concentration, India, Molecular Sequence Data, Paenibacillus isolation & purification, Paenibacillus metabolism, Paper, Phenotype, Phylogeny, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Soil chemistry, Soil Pollutants, Waste Disposal, Fluid, Water Pollutants, Chemical, Agricultural Irrigation methods, Bacillaceae genetics, Genetic Variation genetics, Industrial Waste, Paenibacillus genetics, Soil Microbiology
Abstract

The genetic and functional diversity of Bacillus and Bacillus-derived genera was analyzed in soil samples collected from three different fields near Century Paper Mill, Lal Kuan, Uttarakhand, India. Two had been subjected to concentrated and diluted effluent irrigation for the past 25 years and were designated as a concentrated effluent irrigated field (CEIF) and a dilute effluent irrigated field (DEIF), respectively. The field irrigated with fresh water was designated as a water irrigated field (WIF). Increase in pH, Na and Zn content and decrease in Fe content was observed due to effluent irrigation. The population count of Bacillus and Bacillus-derived was maximum in DEIF followed by WIF and CEIF. Variations in plant growth-promoting traits and extracellular enzymes were observed among the isolates from the three different field soils. Based on the amplified ribosomal DNA restriction analysis (ARDRA) with three restriction enzymes, all the selected 104 isolates were clustered into 14 groups. The sequencing of the representative isolates revealed that the majority belonged to the genus Bacillus, while three isolates belonged to Paenibacillus, Lysinibacillus and Orthinibacillus. There were a few species like Orthinibacillus contaminans, B. oleronius, B. safensis, B. methylotrophicus, B. stratosphericus, B. aryabhattai, B. asahii and B. bataviensis that were prevalent only in DEIF and CEIF but not in WIF field soil. The diversity index parameters like the Shannon Index, indices of species richness and species evenness based on biochemical profiling and ARDRA profiling revealed that Bacillaceae members in the fresh water irrigated field were diverse.

Published
2011
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2. Genetic and functional diversity of Bacillus strains in the soils long-term irrigated with paper and pulp mill effluent

Author

Dilip K. Arora, Anil Kumar Saxena, Subhash Yadav, and Rajeev Kaushik

Subjects
Paper, Irrigation, Veterinary medicine, Agricultural Irrigation, Molecular Sequence Data, India, Industrial Waste, Bacillus, Fresh Water, Waste Disposal, Fluid, Applied Microbiology and Biotechnology, Microbiology, Soil, Paenibacillus, Diversity index, RNA, Ribosomal, 16S, Botany, Cluster Analysis, Soil Pollutants, Bacillaceae, Effluent, Phylogeny, Soil Microbiology, Base Sequence, biology, business.industry, Genetic Variation, Paper mill, Sequence Analysis, DNA, Hydrogen-Ion Concentration, biology.organism_classification, Amplified Ribosomal DNA Restriction Analysis, Phenotype, Soil water, Species richness, business, Water Pollutants, Chemical
Abstract

The genetic and functional diversity of Bacillus and Bacillus-derived genera was analyzed in soil samples collected from three different fields near Century Paper Mill, Lal Kuan, Uttarakhand, India. Two had been subjected to concentrated and diluted effluent irrigation for the past 25 years and were designated as a concentrated effluent irrigated field (CEIF) and a dilute effluent irrigated field (DEIF), respectively. The field irrigated with fresh water was designated as a water irrigated field (WIF). Increase in pH, Na and Zn content and decrease in Fe content was observed due to effluent irrigation. The population count of Bacillus and Bacillus-derived was maximum in DEIF followed by WIF and CEIF. Variations in plant growth-promoting traits and extracellular enzymes were observed among the isolates from the three different field soils. Based on the amplified ribosomal DNA restriction analysis (ARDRA) with three restriction enzymes, all the selected 104 isolates were clustered into 14 groups. The sequencing of the representative isolates revealed that the majority belonged to the genus Bacillus, while three isolates belonged to Paenibacillus, Lysinibacillus and Orthinibacillus. There were a few species like Orthinibacillus contaminans, B. oleronius, B. safensis, B. methylotrophicus, B. stratosphericus, B. aryabhattai, B. asahii and B. bataviensis that were prevalent only in DEIF and CEIF but not in WIF field soil. The diversity index parameters like the Shannon Index, indices of species richness and species evenness based on biochemical profiling and ARDRA profiling revealed that Bacillaceae members in the fresh water irrigated field were diverse.

Published
2011