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Pressurized intraperitoneal aerosol chemotherapy (PIPAC) is a technique to directly deliver chemotherapeutic drugs in the abdomen for the treatment of peritoneal metastases. Pressurization improves the treatment efficacy but increases the risk of exposure for the medical/non-medical staff who can be exposed by dermal or ocular contact, or inhalation of aerosols containing the cytotoxic drugs. The aim of this study was to evaluate the risk of exposure for the medical/non-medical staff (nurses, surgeons, anaesthesiologists and cleaning personnel; n = 13) during PIPAC with oxaliplatin performed according to the protocol recommended in France. Blood samples were collected 1 h before and immediately after PIPAC, and urine samples 1 h before, and then 3 h and the morning after PIPAC. In the control, non-exposed group (n = 7), only one urine and blood sample were collected. Surface contamination in the operating room was assessed in water- and Surfanios-impregnated wipe samples. The total elemental platinum in each sample was quantified by inductively coupled plasma mass spectrometry, using a method adapted to quantify trace amounts (ng.L−1) in very low volumes (100 μl). No surface contamination was detected. Although 25% of urine samples in the exposed group contained platinum, no statistical difference was observed in urine and plasma samples collected before and after PIPAC and with the control group samples. These findings suggest that the French PIPAC protocol does not increase the risk of exposure to platinum in all staff categories involved. This protocol could be considered in future occupational policies and consensus statements. Trial registration: NCT04014426

International audience; A complementary approach using Liquid Chromatographic-Mass Spectrometric analysis was proposed to characterize phenolic compounds from the methanol-water extracts of K. ivorensis A. Chev. Two High Performance Liquid Chromatography (HPLC) complementary methods were used for the determination of phenolic compounds from the bark, sapwood and heartwood of K. ivorensis. Methods employed involved direct analysis after filtration at 0.20 µm, using a RP C18 column and UV-VIS/ESI-FTMS detection. The methods used were different by their elution gradient and allowed analyzing the chemical composition of three parts of African mahogany extracts. In this study, 22 phenolic compounds and derivatives from K. ivorensis were separated, determined or tentatively characterized for the first time based on their methanol/water/formic acid extract mass spectra. The difference of gradients eluted various compounds, 8 were obtained with the first method and 14 with the second one. The main products were hydroxybenzoic acid and derivatives, resorcinol, esterified compounds, mannitol, quercetin and derivatives, dihydroxyflavan, and trihydroxyflavan.

International audience; A new total consumption micronebuliser for ICP-MS was developed and optimised in this work. This nebuliser, V64-01, which was built through 3D printing, contains a larger internal diameter than other total consumption micronebulisers, a silicon-free capillary that perfectly tolerates a flow up to 65 μL min-1 THF, and the chances of incurring obstructions or clogging during a prolonged period of analysis are reduced. In addition, its production costs are minimal. To validate this nebuliser, its most important parameters were optimised (carrier gas and liquid flow rates), and the quantitative analysis of an SRM sample was carried out successfully. Calibration curves with great linearities (r2 > 0.999) and detection limits between 0.84 and 2.85 ng g-1 were obtained for the analysis of 10 elements. In addition, GPC-ICP-MS chromatograms of the size distribution of V and Ni species in a reference crude oil sample were obtained, showing the same profile as with the previous nebulisers but also suggesting that, for some species of V and Ni in crude oil, permanent retention might have occurred within the fused silica capillary that connects the DS-5 total consumption micronebuliser with the HPLC system. This new 3D-printed total consumption nebuliser possesses the potential to become a good consumable that will allow for total and speciation analysis of numerous trace elements, such as Si, that, until now, due to either interactions with silica or interferences, were not possible to analyse.

International audience; In the search for an alternative strategy to the radioactivity measurement conventionally performed to probe receptor–ligand interactions in pharmacological assays, we demonstrated that selenium labeling of the studied ligand combined with elemental mass spectrometry was as efficient and robust as the reference method but devoid of its environmental and health hazards. The proof-of-concept was illustrated on two GPCR receptors, vasopressin (V1A) and cholecystokinin B (CCK-B), involving peptides as endogenous ligands. We proposed several methodologies to produce selenium-labeled ligands according to peptide sequences along with binding affinity constraints. A selection of selenopeptides that kept high affinities toward the targeted receptor were engaged in saturation and competitive binding experiments with subsequent sensitive RP-LC-ICP-MS measurements. Experimental values of affinity constant (Ki) were perfectly correlated to literature data, illustrating the general great potency of replacing radioactive iodine by selenium for ligand labeling to further undergo unaffected pharmacology experiments efficiently monitored by elemental mass spectrometry.

cited By 0; International audience; Chloride is often considered as the main chlorine form in soils. However, recent studies show that chlorine is mostly present in soils as naturally produced organically bound molecules. The relative contribution of biotic, including microbial, and abiotic processes to formation of organically bound chlorine remains poorly understood. We performed a37Cl spiking batch experiment with a forest soil incubated under abiotic and biotic conditions over two time periods to simultaneously monitor the formation of organically bound chlorine from natural and tracer chlorine. To compare biotic and abiotic conditions without biased effect of sterilization technique for abiotic control, the soil was irradiated and reinoculated or not with soil microflora. Fifteen days after microbial inoculation, the natural non-extractable organic chlorine content in the inoculated soil was significantly higher than in the sterile soil, showing that microbial activity contributed to formation of organically bound chlorine. However, no significant difference was noted between the two incubation periods. The same trend was noted for tracer chlorine, yet without a significant difference. The present study shows that chlorination is mediated by microbial activity, but there is also some indication of abiotic formation of organically bound chlorine, with a non-extractable organic tracer chlorine formation of about 6% just after spiking in abiotic conditions. © 2016 Elsevier Ltd

International audience; The size distribution of sulfur, vanadium, and nickel was determined for four crude oils and their distillation cuts using gel permeation chromatography (GPC) coupled to inductively coupled plasma high-resolution mass spectrometry (ICP HR MS). The results show a trimodal distribution of vanadium and nickel compounds in the crude oils, the atmospheric residues, and the vacuum residues and, for sulfur compounds, either a mono- or bimodal distribution depending upon the distillation cut considered. A correlation exists between the sulfur fraction retention times and the temperature cuts of the distillation for a temperature below 560 degrees C and also between the viscosity of the crude oils and the proportion of trapped sulfur compounds in a higher boiling temperature fraction. The thermic treatment applied for the distillation increases the aggregation of low- and medium-molecular-weight compounds of vanadium and nickel into higher molecular weight aggregates between the crude oil on the one hand and the atmospheric residue and vacuum residue on the other hand, especially when the crude oil has a high total sulfur content.

International audience; A new high performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS) method was developed for a simultaneous multi-residue analysis of 22 sulfonamides (SAs) and their metabolites in edible animal (pig, beef, sheep and chicken) tissues. Sample preparation was optimized on the basis of the "QuEChERS" protocol. The analytes were identified using their LC retention times and accurate mass; the identification was further confirmed by multi-stage high mass accuracy (

International audience; Rice is a staple food for nearly half the world’s population. The discrimination of geographical origin of rice in order to its authenticity is essential to prevent mislabeling and adulteration problems. The multi-element fingerprinting has a great potential for the differentiation of rice grains. A study of the capability of the high resolution inductively coupled plasma mass spectrometry (HR-ICP-MS) methodology for multi-element fingerprinting of rice has been carried out. A total of 31 Thai jasmine rice and 5 foreign (France, India, Italy, Japan and Pakistan) rice samples were analysed by high resolution ICP-MS after acid digestion. Accuracy of the whole procedure was verified by the analysis of rice flour standard reference material (NIST SRM 1568a). The concentrations of 21 elements were evaluated and used as chemical indicator to discriminate the origin of rice samples. The classification of rice samples was carried out based on elemental composition by a radar plot and multivariate data analysis, including principal component analysis (PCA) and discriminant analysis (DA). Thai jasmine rice can be differentiated from foreign rice samples by radar plots and multivariate data analysis. Furthermore, the DA can differentiate Thai jasmine rice samples according to each region of origin (northern, northeastern or central regions of Thailand). Therefore, multi-element fingerprinting combined with the use of multivariate statistical techniques can be considered as a powerful tool for rice authentication.

International audience; A novel method for the preparation of crude oil samples based on a xerogel matrix was developed for elemental analysis using laser ablation-double focusing sector field inductively coupled plasma-mass spectrometry. The samples and aqueous solution were successfully encapsulated using zirconium n-propoxide. The use of xerogel produces stable and homogeneous pellets, avoids splashing of the sample and allows the use of aqueous inorganic standards to elaborate the calibration curve. Sulfur, nickel and vanadium were selected as test elements and indium was used as the internal standard to correct the variations in the amount of material ablated. The correlation coefficients of the calibration curves for all the elements tested were better than 0.990 except for sulfur. Crude oil results showed no significant statistical differences between the sol-gel results and those obtained with similar studies. © 2012 The Royal Society of Chemistry.

International audience; One of the factors that may explain nowadays honeybees' colonies losses is the increasing presence of chemicals in the environment. The aim of this study is to obtain a global view of the presence of environmental contaminants in beehives and, develop a fast, cheap and sensitive tool to analyze environmental contaminants in apiarian matrices. A multi residue analysis was developed to quantify 80 environmental contaminants, pesticides and veterinary drugs, belonging to different chemical classes, in honeys, honeybees and pollens. It consists in a single extraction, based on a modified "QuEChERS method", followed by gas chromatography coupled with Time of Flight mass spectrometry (GC-ToF) and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The "QuEChERS method" combines salting-out liquid-liquid extraction with acetonitrile and a dispersive-SPE clean up. It was adjusted to honey and especially to honeybee and pollen, by adding a small fraction of hexane in acetonitrile to eliminate lipids that interfere with mass spectrometry analysis. This method, combined with accurate and sensitive detection, allowed quantification and confirmation at levels as low as 10 ng/g, with recoveries between 60 and 120%. Application to more than 100 samples of each matrix was achieved for a global view of pesticide presence in the honeybee environment. Relatively high percentages of honeys, honeybees and pollens were found to be contaminated by pesticides used to combat varroa but also by fungicides like carbendazim and ubiquitous contaminants.

International audience; A multi residue analysis was developed for screening, quantification and confirmation of 36 priority organic compounds included in the 2000/60/EC European Water Framework Directive. The compounds analyzed included 19 pesticides, 8 PAH, 5 endocrine-disruptors and 4 organochlorine compounds. The method was developed in three steps. First, automated off-line solid-phase extraction using Strata X cartridges was optimized to trap simultaneously the 36 studied compounds. Second, the more volatile compounds were analysed by gas chromatography coupled to mass spectrometry with electron impact ionisation in selected ion monitoring mode (SIM). Third, the last 20 compounds were detected and quantified, in one run, by liquid chromatography coupled to fluorescence detector and tandem mass spectrometry. The excellent selectivity and sensitivity allowed us satisfactory quantification and confirmation at levels as low as 0.2-67 ng L(-1) with recoveries between 59 and 105%. Such methodology was then applied to French surface waters: all the waters present organic contaminants, and their concentration varied according to the origin and nature of substances.