Identification of Phenolic Compounds from K. ivorensis by Selected Chromatographic and Spectrometric Techniques

International audience; A complementary approach using Liquid Chromatographic-Mass Spectrometric analysis was proposed to characterize phenolic compounds from the methanol-water extracts of K. ivorensis A. Chev. Two High Performance Liquid Chromatography (HPLC) complementary methods were used for the determination of phenolic compounds from the bark, sapwood and heartwood of K. ivorensis. Methods employed involved direct analysis after filtration at 0.20 µm, using a RP C18 column and UV-VIS/ESI-FTMS detection. The methods used were different by their elution gradient and allowed analyzing the chemical composition of three parts of African mahogany extracts. In this study, 22 phenolic compounds and derivatives from K. ivorensis were separated, determined or tentatively characterized for the first time based on their methanol/water/formic acid extract mass spectra. The difference of gradients eluted various compounds, 8 were obtained with the first method and 14 with the second one. The main products were hydroxybenzoic acid and derivatives, resorcinol, esterified compounds, mannitol, quercetin and derivatives, dihydroxyflavan, and trihydroxyflavan.