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A global phenomenon identified 200 years ago as Urban Heat Island (UHI) effect gained popularity as the sheer contributor to the precipitous temperature gradient between rural and urban interface, instigating excess heat gain and associated ill effects on the urban dwellers. UHI is a function of many interrelated geographical, ecological, and economic parameters that require differential treatment in determining the antecedent impacts. This transdisciplinary review assessed the passive strategies (vegetation, cool roofs, cool pavements, and green roofs) from 83 studies that employed a numerical simulation approach to combat UHI. On average, vegetation and cool/green roofs can reduce ambient temperature by 3–5 ​°C, while cool pavements help to reduce surface temperature by 5 ​°C. All passive strategies also reveal it can reduce buildings' energy demand by 4–10%. However, the current methodological framework for evaluating UHI is quite fragmented, using multiple software and estimates only Surface Urban Heat Island (SUHI), ignoring Canopy Urban Heat Island (CUHI), Boundary Urban Heat Island (BUHI), and the nexus of ‘UHI-Climatology,’ which is linked to regional and global climate change, failing to model UHI and its complex connection to climate change accurately. The review found that the efficacy of passive strategies is a function of factors ranging from location, cloud cover, and soil type to simulation accuracy; hence, while these passive strategies alleviate outdoor temperature in one place, they can cause counterproductive impacts in another region. Therefore, as a postlude, the paper explores an alternative methodological framework for evaluating the nexus of UHI-Climatology using digital twin technology, thus espousing better mitigation strategies.

Phosphorus (P) is considered as an essential element in crop production. Besides, the study of P from the elemental and bio sources impacts on productivity and quality of orange trees could emphasize its utilization importance. This study aimed to evaluate the chemical implication of three treatments of P fertilization (Triple phosphate (TP; 620 and 900 g/tree/year), phosphate rock (PR; 1820 and 2742 g/tree/year) and 1:1 TP:PR on Valencia orange trees (Citrus sinensis L. Osbeckunder). Where, all these treatments were equipped with bio-phosphorus fertilizer (phosphorin) (0 and 5 g/tree/year added twice in the year, Feb. and Jun.). The measurements included leaves nitrogen (N), phosphorus (P) and Potassium (K), number of fruits (NoF), juice weight/volume, total soluble solids (T.S.S.), vitamin C (V.C), and acidity. The results showed that the treatment (50 % TP +50 % PR at 450 + 1371g/tree/year + phosphourin at 5g/tree/year) gave the highest values for all characters i.e. N % in tree leaves 2.61 and 2.69 %, P% in tree leaves 0.16 and 0.17 %, K in tree leaves 1.79 and 1.86 %, number of fruits/tree 412.0 and 420.0, fruit weight 267.0 and 280.0g, fruit weight/tree 110.0 and 117.60 kg, fruit size 260.41 and 272.37 cm3, juice weight 114.1 and 126.15g, fruit juice 44.33 and 44.99 %, juice volume 110.15 and 114.90 cm3, peel thickness 0.58 and 0.60 cm, T.S.S. 12.14 and 12.21 %, T.S.S./Acid ratio 9.07 and 9.41, and V.C 70.15 and 72.44 (mg/100 ml) as well as the lowest total acidity 0.93 and 0.91 %, under both the 1st season and the 2nd one, respectively. In conclusions, as the application of bio-fertilization has improved the quality charcatistics over seasons. Thus, this study opens the way towards the advanced application of bio-P element to understand the biogeochemical cycles, dynamics and function of natural ecosystems elements in agricultural and food security program.

This paper presents the power-on signature graph of analog circuits for fault classification. This graph can be attained using the simulation mechanism through the practical circuit simulator and the hardware mechanism through the mixed-signal design. The presented signature graph is influenced by changes in pass-band transmission and bandwidth as a result of device under test (DUT) component modifications. In order to exercise the frequency band of the DUT for fault stimulation, sinusoidal waveforms wiped at their frequencies are produced using the analog waveform generator (AWG). The analog compactor is devised to accumulate the output samples from the DUT for signature generation, compared with global signature boundaries derived from the worst-case analysis. The built-in self-test controller is devised to properly synchronize the process of analog test cycle for proper signature generation. Two DUTs chosen from a variety of analog circuits in frequency bands used in medical applications are subjected to this testing mechanism. Due to the difference between the wiped sinusoidal frequencies of the AWG in the simulation mechanism and the hardware mechanism, the normalized signature graphs of each component in DUTs using both mechanisms are developed to attain the approved convergences between the two mechanisms.

Grouper fish are among the most important components of the fisheries of many countries because they are found in warm water throughout the world. There are 15 genera and 159 species known worldwide; 8 genera and 66 species are exclusively found in the western Indian Ocean, Red Sea, and Arabian Gulf. The Summan grouper, Epinephelus summana, constitutes a considerable portion of these fisheries; therefore, this study aimed to evaluate the reproductive strategy of this important fish species. The fish samples were collected monthly for one year (from November 2020 to October 2021), and 217 fish were collected from the Red Sea of Jeddah, Saudi Arabia. The sex ratio, sexual maturation process, and spawning season were analyzed. Across all samples, landing consisted of 36.2 ± 4.7 % males, 64.0 ± 5.0 % females, and 3.4 ± 1.8 % transitional-stage fish, with an overall significantly different male-to-female sex ratio of 1:3.3. Furthermore, males were larger than females. The maturation index (MI), gonadosomatic index (GSI), and ovarian maturation rate (OMR) values fluctuated throughout the year, indicating that E. summana has extended spawning and spawns in batches during different months of the year. However, April to May is the main spawning season, with the highest female GSI recorded. Based on the microscopic histological examination of gonads, the maturation process can be classified into five stages in both males and females. In conclusion, this fish species has a complex reproductive biology. It undergoes sexual transformation and protogynous hermaphroditism, during which individuals mature first as female and then change sex to male. The obtained data is essential for successful fishery stock conservation, management, and aquaculture development.

Entropy generation(EG) has attracted the attention of scientists and researchers worldwide due to its applications in the industrial and engineering sectors. Applications of entropy generation can be seen in chillers, air conditioners, air separates, air coolers, nuclear reactors, and vehicle engines. In the current investigation, we have analyzed entropy generation in Maxwell hybrid nanofluid(HNF) flow over a porous stretching sheet. Darcy Forchheimer(DF) relation is considered. Due to stretching of sheet flow generates. The thermal expression is reported taking influences of dissipation, radiation, and heat source. The second law of thermodynamics is utilized to obtain EG for considered flow. Boundary layer assumptions are considered in the modeling. Partial differential equations(PDEs) governing the flow are changed into dimensionless ordinary differential equations(ODEs) through transformations. The solution of reduced ODEs is constructed via NDSolve code in Mathematica software. Different influential flow variables impact on velocity, temperature, irreversibility, and Bejan number are discussed graphically. Physical quantities are examined numerically. The results show that velocity diminished for rising porosity and Forchheimer variables. Temperature field upsurges for an upturn in radiation and Eckert numbers, an opposite impact is noticed for higher Prandtl number. EG escalates for greater radiation, temperature, and Brinkman variables.

Objective: To develop and internally validate a nomogram to predict recurrence-free survival (RFS) including the time to radical cystectomy (RC) and perioperative blood transfusion (PBT) as potential predictors. Methods: Patients who underwent open RC and ileal conduit between January 1996 to December 2016 were split into developing (n=948) and validating (n=237) cohorts. The time to radical cystectomy (TTC) was defined as the interval between the onset of symptoms and RC. The regression coefficients of the independent predictors obtained by Cox regression were used to construct the nomogram. Discrimination, validation, and clinical usefulness in the validation cohort were assessed by the area under the curve, the calibration plot, and decision curve analysis. Results: In the developing dataset, the 1-, 5-, and 10-year RFS were 83.0%, 47.2%, and 44.4%, respectively. On multivariate analysis, independent predictors were TTC (hazards ratio [HR] 1.07, 95% confidence interval [CI] 1.05–1.08, p

In this study, a series of bis amide thiazolidine derivatives (Q1-Q6) were synthesized and their anticancer activity was evaluated against prostate (PC3) and breast (MCF7) cancer cells and normal cells line activity was evaluated against breast (MCF10), prostate (PNT1A) and living human cells (HUVEC) cancer cells. The thiazolidine rings were built from penicillamine and aromatic aldehydes (A1-A6), then converted to acetyl thiazolidines (B1-B6) using acetic anhydride, and finally linked with phenylene diamine to form the final compounds (Q1-Q6). Notably, compounds Q1 and Q3 displayed the highest activity against PC3, with IC50 values of 81 and 89 µg/ml, respectively. Docking simulations were performed for Q1, Q4, and Q5 against protein structures related to cancer (2FVD and 1SJ0). Additionally, DFT calculations were used to determine various molecular properties like HOMO/LUMO energies, band gap, and other descriptors, providing insights into the compounds’ stability and reactivity.

Several genotypes of the highly pathogenic avian influenza (HPAI) virus H5N8 subtype within clade 2.3.4.4b continue to circulate in different species of domestic birds across Egypt. It is believed that quail contribute to virus replication and adaptation to other gallinaceous poultry species and humans. This study provides genetic characterization of the full genome of HPAI H5N8 isolated from quail in Egypt. The virus was isolated from a commercial quail farm associated with respiratory signs. To characterize the genetic features of the detected virus, gene sequencing via Sanger technology and phylogenetic analysis were performed. The results revealed high nucleotide identity with the HPAI H5N8 virus from Egypt, which has multiple basic amino acid motifs PLREKRRKR/GLF at the hemagglutinin (HA) cleavage site. Phylogenetic analysis of the eight gene segments revealed that the quail isolate is grouped with HPAI H5N8 viruses of clade 2.3.4.4b and closely related to the most recent circulating H5N8 viruses in Egypt. Whole-genome characterization revealed amino acid preferences for avian receptors with few mutations, indicating their affinity for human-like receptors and increased virulence in mammals, such as S123P, S133A, T156A and A263T in the HA gene. In addition, the sequencing results revealed a lack of markers associated with influenza antiviral resistance in the neuraminidase and matrix-2 coding proteins. The results of the present study support the spread of HPAIV H5N8 to species other than chickens in Egypt. Therefore, continuous surveillance of AIV in different bird species in Egypt followed by full genomic characterization is needed for better virus control and prevention.

Kaolin soil is found in various places in Egypt, such as the Sinai Peninsula, Al-Galala, and Aswan. The need to improve kaolin soil is urgent because of its low to moderate bearing capacity. The effect of fly ash on the enhancement of low-plasticity kaolin soils is the main purpose of this work. Since fly ash requires alkali activation to enhance its pozzolanic reactivity, a small amount of hydrated lime (3 %) has been added to fly ash. The behaviour of untreated and treated kaolin soil with fly ash proportions ranging from 5 % to 20 % by dry weight of soil is studied and analysed. Unconfined compressive strength (UCS), Atterberg limits, compaction, and California bearing ratio (CBR) tests are conducted on low-plasticity Kaolin clay and binder mixtures. Results of more than 120 tests indicate that the optimum moisture content (OMC) decreases while the maximum dry density (MDD) increases by adding binders to kaolin soil. It is also noticed that the maximum improvement of the CBR occurs by adding 3 % lime and 10 % fly ash to kaolin soil. The results of the UCS test show that using 5 % and 10 % fly ash with 3 % lime gives low UCS compared to adding only 3 % lime for all curing periods. On the other hand, using 15 % and 20 % fly ash with 3 % lime gives a significant increase in UCS compared with other studied cases.

This study aims to use hot aqueous extract of herbal residue (HRE) to enhance bacterial growth and possess anti-diarrheal effects. In this study, lactobacillus species L. brevis (SAM-1), Lactobacillus plantarum (SAM-2), and Lactobacillus herbinensis (SAM-3) were isolated from date palm sap (collected in winter season). Square Pharmaceuticals PLC, Bangladesh provided herbal residues ‘Adovas’ which is non-sedating herbal cough syrup with sixteen common herbs including Adhatoda vasica. In our observation, HRE increased the number of colonies in MRS media. In the anti-diarrheal study by castor oil and magnesium sulphate-induced diarrheal mouse model, SAM-1 and 2 with or without HRE showed almost similar results. After initial morphological characterization, tests such as resistance to low pH, bile salt and survival capability in gastric simulated fluid (GSF) were performed to confirm them as a probiotic candidate. All three isolates were gram-positive bacteria and could grow in a mesophilic range of temperatures. The isolates were catalase-negative and were able to coagulate milk after overnight incubation. As the isolates exhibited resistance to low pH and could tolerate bile salts, they may survive in the stomach and intestine, thus making them a promising probiotic candidate. The isolated probiotics and HRE inhibited diarrheal and restored the body's electrolytes. Interestingly, SAM-2 showed higher efficacy than the standard drug (Loperamide), while SAM-1 showed a similar effect and SAM-3, had less effect than Loperamide. The findings suggest that probiotics and herbal residue could contribute to diarrhoeal disease prevention, which might be an alternative to a synthetic standard drug (Loperamide).

The increasing incidence of resistance extended spectrum-beta lactamase (ESBL) producing Klebsiella pneumonia become worldwide issue. The current study aimed to determine the genomic diversity of ESBL-producing K. pneumoniae in milk samples collected from cows with mastitis as well as their antibiotic sensitivity profiles and genetic identification in Peshawar, Pakistan. The california mastitis test (CMT) was initially used to verify the presence for mastitis in 700 collected milk samples. The molecular identification of the 16SrRNA gene confirmed 120/700 (17.14 %) propagation of K. pneumonia. Out of these isolates MDR ESBL-producing isolates were 60/120 (50 %). The lactose were found (M = 3.96 ± 0.28, SD = 2.19), followed by fats (M = 3.12 ± 0.11, SD = 0.90), protein (M = 5.97 ± 0.24, SD = 1.84), sodium (M = 55.74 ± 2.07, SD = 15.81), potassium (M = 138.5 ± 1.53, SD = 11.71), chloride (M = 0.74 ± 0.03, SD = 0.24), calcium (M = 10.27 ± 0.31, SD = 2.42), and chlorine (M = 2.80 ± 0.22, SD = 1.70), respectively. Amikacin (80 %), ceftazidime (71 %), and tetracycline (71 %) were shown to be the most effective antimicrobials against all of the isolates. The occurrence of the blaSHV gene was observed at 56.00 % whereas the blaTEM gene and blaCTX-M gene were 36.00 %, and 30.00 %. The distribution of blaCTX-M subgroup genes was followed by blaCTX-M-1 (38.00 %), blaCTX-M-9 (22.20 %), and blaCTX-M-15 (61.10 %). Co-occurrence of blaCTX-M+ blaSHV was (15.00 %), blaCTX-M+ blaTEM were (6.60 %), and blaSHV + blaTEM were (10.00 %), respectively. The inappropriate, prolonged and common use of antibiotics may apply selective pressure for propagation and the occurrence of resistant isolates.

The performance of polycrystalline solar modules in semi-arid regions, such as Aswan City, Egypt, can be significantly influenced by local climatic conditions. This study aims to develop simplified models to accurately predict the thermal and electrical performance of these modules. We propose three models that directly correlate incident solar radiation with temperature under clear sky conditions. These models were validated against nominal operating cell temperature values, showing strong agreement. Our results indicate electrical efficiencies between 17.24 % and 15.92 %, and exergy efficiencies from 16.64 % to 14.53 %. The energy payback time was determined to be 2.17 years. Additionally, the PV system demonstrated a sustainability index above one and is expected to mitigate approximately 14.4 tons of CO2 emissions per square meter over a 25-year lifespan. Compared to complex PV models, our simplified models offer practical advantages, advancing the United Nations Sustainable Development Goals by enhancing solar energy efficiency, promoting economic growth, fostering sustainable cities, and contributing to climate action in Aswan.

Using porous media is an efficient technique aimed at enhancing heat transfer in engineering systems. Replacing conventional fluids by ferrofluids and applying external magnetic fields through magnets is another method for heat transfer control. In this regard, understanding the behavior of ferrofluids through porous media in the presence of magnets is important. The present study aims at providing a thorough analysis of the properties of heat transfer resulting from the combination of buoyancy driven convection and thermomagnetic convection in a porous enclosure. The equations governing the two types of convection in the fluid as well as the conduction in the solid matrix are presented in the dimensionless form and solved numerically. The control volume finite element method has been used to solve the governing equations. The influence of various parameters such as the magnetic Rayleigh number, the porosity, Darcy number, and the interfacial heat transfer coefficient on the flow and temperature contours and on the Nusselt number is then investigated. For low interface coefficient, the heat transfer in the fluid is enhanced when the overall porosity in the cavity is raised, while for high coefficients, the porosity of the bottom porous layer is the main parameter affecting the heat transfer.

In this study, the structural performance of reinforced concrete frames strengthened with external post-tension tendons was investigated. An experimental study was performed to investigate the failure modes and generate the data required to validate the numerical model. The experimental study includes a reinforced concrete (RC) frame control specimen without strengthening and three strengthened specimens under vertical loads. The specimens were strengthened using external post-tensioning with three different tendon layouts, including a straight tendon in the beam-positive moment zone, straight tendons in the beam-positive and column-negative moment zones, and U-shape tendons along the frame beam. The numerical model was built for the four specimens, validated using the experimental results, and used to analyze the lateral load performance. Results showed that the most effective strengthening technique for the vertical load was the beam U-shape tendons, and for the lateral load, it was the beam and column strengthening. It can be concluded from this case study that strengthening frames with external post-tensioning techniques can enhance the first crack load, yielding load, and ultimate load by 80–100 %, 180–250 %, and 35–70 %, respectively.

This paper concerns the study of the expansion joints of Benha Bridge under seismic loads. The seismic behaviour of its bridge expansion joints has been enhanced by shape memory alloy (SMA) dampers as a form of energy-dissipation device. SMA can dissipate a part of the input energy from the earthquake by generating a counterforce for the induced seismic force, allowing the opportunity to control the joint width and overcome pounding and unseating problems. In this research, MATLAB programs are developed to calculate the joint width responses in the two cases of without SMA and with SMA control devices embedded between adjacent bridge frames, which are modeled as a single degree of freedom system for each frame. Benha Bridge is the subject of this case study, which aims to check and validate the SMA parameter values that their charts have introduced from earlier research and to improve the seismic behaviour of bridge expansion joints by eliminating unseating and pounding problems. The results show that SMA dampers are able to control the joint width and absorb the excess forces caused by nearby expansion joint overlap. Furthermore, SMA design charts are applicable for bridges of multiple frames when they are homogenous in time periods.

Silymarin (SLR) is a poorly water-soluble bioactive compound with a wide range of therapeutic activities. Nanosized silymarin vesicles (F1–F6) were prepared by the solvent evaporation rehydration method. The silymarin vesicles were evaluated for vesicle size, surface charge, entrapment efficiency, and drug release studies. The optimized SLR lipid vesicle (F3) was further modified with the addition of the cationic polymer chitosan. After that, the modified vesicle (F3C1) was assessed for permeation flux, antimicrobial activity, cell viability, and molecular docking studies. The silymarin vesicles showed nanometric size (

Anaerobic co-digestion offers a promising approach to enhancing biogas production from surplus by-products of wastewater treatment plants (WWTPs) and agricultural waste while reducing environmental negative impact. This study investigated the co-digestion of anaerobic thickened sewage sludge (ATSS) and rice straw (RS) under mesophilic conditions (35 ± 1 °C). In phase 1 of the experiment, lab-scale reactors with varying MRs of RS (0 %, 4 %, 8 %, and 16 % based on sludge weight) were employed to determine the optimal MR for maximizing biogas quantity and methane content. Phase 2 examined the impact of mechanical mixing (MM) on biogas production, pathogen and trace element reduction, and the calorific value (CV) of the co-digested mixture compared to the digested thickened sludge (DTS) and RS individually to assess the potential energy of co-digestion. Results revealed that the 8 % MR yielded the highest biogas volume, approximately 2.5 times that of the DTS alone, with methane and carbon dioxide contents within the typical ranges. MM in phase 2 further increased biogas volume by 2.8 times compared to DTS. The CV of the co-digested mixture was 24,937 kJ/kg and 21,929 kJ/kg for the DTS. Pathogens were effectively eliminated, and trace elements remained below the permissible limits. The 8 % MR with MM was identified as the optimal MR that produces the highest biogas quantity before reaching the turning point. Moreover, co-digestion at this MR enhanced the CV of ATSS by 14 %.

Hydrogen provides the greatest performance in conditions of capacity and duration when related to other energy storage techniques. Renewable energy sources including solar, geothermal, wind, wave and tidal energy, and hydropower, are used to create green hydrogen. However, biogas is converted into renewable hydrogen via chemical procedures. A comparison of several water Electrolyzer types is provided by this research. All kinds of Electrolyzers have an impact on how well a green hydrogen production system works were discussed. This review gives a broad review of environmentally friendly hydrogen generation techniques based on renewable energy sources. These sources incorporate solar energy, hydropower, wind energy, tidal and wave energy, biogas systems, geothermal energy, and hybrid systems of these sources. The method used to obtain the hydrogen and its effects on the environment is the primary distinction between green hydrogen and other colors. Climate, control, management systems, and tracking systems. The innovations and difficulties that green hydrogen systems encounter are highlighted. In the final section, the costs associated with manufacturing hydrogen are explained through an economic study of the understudied production systems LCOE and LCOH. Furthermore, the advantages and disadvantages of all electrolyzers are discussed.

DNA methylation is one of induced changes under salinity stress causing reduction in the expression of several crucial genes required for normal plant's operation. Potential use of royal jelly (RJ), folic acid (FA) and 5-azacitidine (5-AZA) on two Egyptian faba bean varieties (Sakha-3 and Giza-716) grown under saline conditions was investigated. Salinity stress affects negatively on seeds germination (G %), mitotic index, membrane stability and induced a significant increase in chromosomal abnormalities (CAs). DNA methyltransferases genes (MT1 and MT2) were highly up-regulated (∼23 and 8 folds for MT1 and MT2 in shoots of Giza-716 stressed plants). On the other hand, down regulation of other studied stress related genes: superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), heat shock protein (HSP-17.9) and proline-rich protein (GPRP) were detected in stressed plants of both studied varieties. Treating plants with RJ and FA increase G%, chlorophyll content, improves membrane properties and reduces CAs compared to non-treated stressed plants. Exogenous application of 5-AZA, RJ and FA on salinity stressed plants was associated with a significant reduction in the transcription of MT1 and MT2 which was associated with significant up regulation in the expression of Cu/Zn-SOD, CAT, GR, GPRP and HSP-17.9 encoding genes. The Lowest expression of MT1 and MT2 were induced with 5-AZA treatment in both studied varieties. Exogenous application of the FA, RJ and 5-AZA modified the methylation state of stressed plants by regulation the expression of DNA methyltransferases, subsequently, modulated the expression of studied genes and could be proposed as a promising treatment to ameliorate hazardous effects of salt stress on different plants.

In this study, numerical analysis of a porous block influence on the mixed convection in a cavity filled with nanfluid has been investigated. The cavity is curvilinear shaped, in which the cold side is the top which is split to move in two directions on the same plane. The base is partially heated and connected to a block of porous media, while other walls are adiabatic. The complex governingequations are solved numerically using the finite element technique. For the assessment of the thermal performance, the considered variables which are Richardson number (from 0.15 to 10), Reynolds number (from 25 to 200), Darcy number (from 10−5 to 10−1), Hartmann number (from 0 to 62), ma magnetohydrodynamic's inclination angle (from 0° to 90º) and nanoparticles concentration (from 0 to 0.08). The height of the porous block is also investigated. The results’ main finding stated that the heat transfer could be enhanced due to the rise in Reynolds number, Richardson number, and the volume concentration while decreasing with the rise of Hartmann number. Also, value of Nuavg and the streams’ strength increase with the increase in Darcy number.

Pesticides are chemical constituents used to prevent or control pests, including insects, rodents, fungi, weeds, and other unwanted organisms. Despite their advantages in crop production and disease management, the use of pesticides poses significant hazards to the environment and public health. Pesticide elements have now perpetually entered our atmosphere and subsequently contaminated water, food, and soil, leading to health threats ranging from acute to chronic toxicities. Pesticides can cause acute toxicity if a high dose is inhaled, ingested, or comes into contact with the skin or eyes, while prolonged or recurrent exposure to pesticides leads to chronic toxicity. Pesticides produce different types of toxicity, for instance, neurotoxicity, mutagenicity, carcinogenicity, teratogenicity, and endocrine disruption. The toxicity of a pesticide formulation may depend on the specific active ingredient and the presence of synergistic or inert compounds that can enhance or modify its toxicity. Safety concerns are the need of the hour to control contemporary pesticide-induced health hazards. The effectiveness and implementation of the current legislature in providing ample protection for human health and the environment are key concerns. This review explored a comprehensive summary of pesticides regarding their updated impacts on human health and advanced safety concerns with legislation. Implementing regulations, proper training, and education can help mitigate the negative impacts of pesticide use and promote safer and more sustainable agricultural practices.

Purpose: Oral diethylnitrosamine (DEN) is a known hepatocarcinogen that damages the liver and causes cancer. DEN damages the liver through reactive oxygen species-mediated inflammation and biological process regulation. Materials and methods: Gallic acid-coated zinc oxide nanoparticles (Zn-GANPs) were made from zinc oxide (ZnO) synthesized by irradiation dose of 50 kGy utilizing a Co-60 γ-ray source chamber with a dose rate of 0.83 kGy/h and gallic acid from pomegranate peel. UV–visible (UV) spectrophotometry verified Zn-GANP synthesis. TEM, DLS, and FTIR were utilized to investigate ZnO-NPs' characteristics.Rats were orally exposed to DEN for 8 weeks at 20 mg/kg five times per week, followed by intraperitoneal injection of Zn-GANPs at 20 mg/kg for 5 weeks. Using oxidative stress, anti-inflammatory, liver function, histologic, apoptotic, and cell cycle parameters for evaluating Zn-GANPs treatment. Results: DEN exposure elevated inflammatory markers (AFP and NF-κB p65), transaminases (AST, ALT), γ-GT, globulin, and total bilirubin, with reduced protein and albumin levels. It also increased MDA levels, oxidative liver cell damage, and Bcl-2, while decreasing caspase-3 and antioxidants like GSH, and CAT. Zn-GANPs significantly mitigated these effects and lowered lipid peroxidation, AST, ALT, and γ-GT levels, significantly increased CAT and GSH levels (p

Balanites aegyptiaca (B. aegyptiaca) is an African herb with traditional medical applications. Various pathogenic factors cause hepatic fibrosis and require novel treatment alternatives. Nanoformulation-based natural products can overcome the available drug problems by increasing the efficacy of natural products targeting disease markers. The current study investigated B. aegyptiaca methanolic extract using high-pressure liquid chromatography (HPLC), and B. aegyptiaca/chitosan nanoparticles were prepared. In vivo, evaluation tests were performed to assess the curative effect of the successfully prepared B. aegyptiaca/chitosan nanoparticles. For 30 days, the rats were divided into six groups, typical and fibrosis groups, where the liver fibrosis groups received B. aegyptiaca extract, silymarin, chitosan nanoparticles, and B. aegyptiaca/chitosan nanoparticles daily. In the current investigation, phenolic molecules are the major compounds detected in B. aegyptiaca extract. UV showed that the prepared B. aegyptiaca /chitosan nanoparticles had a single peak at 280 nm, a particle size of 35.0 ± 6.0 nm, and a negative charge at − 8.3 mV. The animal studies showed that the synthetic B. aegyptiaca/chitosan nanoparticles showed substantial anti-fibrotic protective effects against CCl4-induced hepatic fibrosis in rats when compared with other groups through optimization of biochemical and oxidative markers, improved histological changes, and modulated the expression of Col1a1, Acta2 and Cxcl9 genes, which manage liver fibrosis. In conclusion, the current research indicated that the prepared B. aegyptiaca/chitosan nanoparticles improved histological structure and significantly enhanced the biochemical and genetic markers of liver fibrosis in an animal model.

This study presents the Jellyfish Search Optimizer (JS), a novel metaheuristic optimization algorithm, for the efficient allocation of multi-type distributed generations (DGs) in distribution power systems. To further enhance the performance of the original JS algorithm, a leader-based mutation-selection approach called LJS is proposed to circumvent local optima. The effectiveness of LJS is evaluated with various benchmark problems and compared with other competitive optimization algorithms. Moreover, LJS is utilized to allocate different types of DGs (Type I, Type II, and Type III) in standard IEEE and practical Portuguese distribution systems. The optimization problem of the DG allocation is performed to minimize the power loss and enhance the voltage profile by minimizing the voltage deviation (VD) and maximizing the voltage stability index (VSI) as single- and multi-objectives optimization problems. The results obtained demonstrate the superior performance of LJS in achieving optimal solutions for benchmark problems as well as for the allocation of multi-type DGs. Notably, the integration of DG Type III leads to a remarkable reduction in total power loss, achieving a reduction of 94.44 %, 98.10 % and 96.07 in IEEE 33-bus,IEEE 69-bus and 94 bus systems, respectively.

Objectives: We assessed short-, medium-, and long-term all-cause mortality risks after a primary SARS-CoV-2 infection. Methods: A national, matched, retrospective cohort study was conducted in Qatar to assess risk of all-cause mortality in the national SARS-CoV-2 primary infection cohort compared with the national infection-naïve cohort. Associations were estimated using Cox proportional-hazards regression models. Analyses were stratified by vaccination status and clinical vulnerability status. Results: Among unvaccinated persons, within 90 days after primary infection, the adjusted hazard ratio (aHR) comparing mortality incidence in the primary-infection cohort with the infection-naïve cohort was 1.19 (95% confidence interval 1.02-1.39). aHR was 1.34 (1.11-1.63) in persons more clinically vulnerable to severe COVID-19 and 0.94 (0.72-1.24) in those less clinically vulnerable. Beyond 90 days after primary infection, aHR was 0.50 (0.37-0.68); aHR was 0.41 (0.28-0.58) at 3-7 months and 0.76 (0.46-1.26) at ≥8 months. The aHR was 0.37 (0.25-0.54) in more clinically vulnerable persons and 0.77 (0.48-1.24) in less clinically vulnerable persons. Among vaccinated persons, mortality incidence was comparable in the primary-infection versus infection-naïve cohorts, regardless of clinical vulnerability status. Conclusions: COVID-19 mortality was primarily driven by an accelerated onset of death among individuals who were already vulnerable to all-cause mortality, but vaccination prevented these accelerated deaths.

This paper exploits a sparse array representation, a novel nested arc array, to generate a virtual non-uniform circular arrays that have been presented and investigated. To increase the array aperture, the virtual array has been developed using higher-order statistics, namely, the Forth-Order Cumulants (FOC). The extended array contains a combination of physical as well as virtual sensors. The increased degrees of freedom (DOFs) in the resultant virtual array make it suitable for over-and under-determined direction of arrival (DOA) estimation. The Cramer-Rao bound (CRB) and the (DOA) estimation for such an array are developed and investigated for various arrays. The proposed array performance is superior to that of the uniform one. Further, the offered array is suitable for two-dimensional (2D) DOA estimation problems.

The durum wheat (Triticum turgidum L. ssp. durum Desf.) Landraces are a valuable source of natural germplasm for enhancing the genetic diversity of existing durum wheat cultivars. The study analyzed the genetic diversity of twenty-two durum wheat landraces including eight local Saudi landraces and fourteen introduced landraces using sixteen morphological descriptors and sequence-related amplified polymorphism (SRAP) markers. The results showed significant differences between the analyzed agro-morphological parameters and a large degree of genetic variation across the tested landraces. Principle components analysis (PCA) was used to determine agro-morphological relationships among different traits. The first six principal components (PCs) accounted for 78.47 % of the cumulative variability among 22 landraces. The PC1demonstrated variability in coleoptile anthocyanin coloration, straw pith, culm neck glaucosity, flag leaf glaucosity, ear glaucosity, and length of awn at tip relative to length of ear. Wheat landraces (L8, L14, L497, L569, L590, L594, L600, L601, and L602) showed increased diversity in dry region and contributed to PC1. A couple landraces had higher plant height (L12, L598, and L654), heading days (650, 656), and ear length (L12, L594, and L650) respectively. The dendrogram generated from the standardized morphological data separated the twenty-two durum wheat genotypes mainly into two clusters. The landraces 14, 600 and 602 grouped into separate cluster with strong correlation to ear glaucosity. Genetic diversity of these landraces was also evaluated using eight SRAP markers, with 482 amplified fragments and an average polymorphic information content (PIC) of 0.27. The dendrogram based on SRAP markers separated into two clusters with some diversified landraces (L8, 594, 602, 977, and L650). The Mantel test found no significant correlation (phenotypic or genetic) between 22 wheat durum landraces (r = 0.013, p = 0.84, =0.05). The results demonstrated the power of morphological and SRAP markers for detecting diversity among durum wheat landraces in the semi-arid region of Saudi Arabia. Thus, study provides insights into wheat diversity for better in-situ and ex-situ conservation and paves the road for their use in a Saudi durum wheat breeding program.

To improve the water quality of Lake Burullus, Delft3D FM hydrodynamic model was applied to simulate the flow and transport models in the Lake. Both the in situ field data and the Lake up-to-date geomorphology were used to get a clear-cut figure. Four different scenarios were applied. The most appropriate solution was obtained from the fourth scenario that suggested constructing a new channel at the northwestern corner of the Lake connecting it to the open Mediterranean Sea and clearing some existing islands and reeds to enhance the free movement of the water among the entire Lake, especially in the western basin.

Candida albicans, an opportunistic pathogen, is the most common type of fungus and represents a substantial source of human invasive disease (nosocomial infection). This category of fungi are part of our microbiota, and given the appropriate environmental conditions, it has the potential to cause both superficial and systemic infections. There is a soaring resistance against the available anticandidal agents. The purpose of this research is to investigate the activity of certain previously synthesized benzo[g]quinazolines against C. albicans in vitro by using the cup-plate diffusion method. There was a marked difference in the effectiveness of the target compounds 1–6 against the sample of C. albicans that was tested. Benzo[g]quinazolines 1 (inhibition zone = 20 mm) and 2 (inhibition zone = 22 mm) had good effects in comparison to fluconazole (inhibition zone = 26 mm). A docking study was conducted between benzo[g]quinazolines 1–6 and Candida spp. CYP51 to establish the binding mode compared with fluconazole and VT-1161 (oteseconazole) as reference medicines, and it was determined that binding at the active site of Candida spp. CYP51 occurred in the same manner. Quantitative structure–activity relationship (QSAR) investigation was performed to further characterize the identified anticandidal agents and recognize the major regulatory components governing such activity. In future studies, the benzo[g]quinazoline scaffold could serve as a model for the design and development of novel derivatives with antifungal potential.

The aim of the optimization economic load dispatch (ELD) problem is to assign the optimal generated power of the thermal units for cost reduction with satisfying the loading of the operational constraints. The ELD is a high-dimensional and non-convex problem that became a more complex problem in the case of optimizing the output generated power of large-scale systems. In this regard, an enhanced version of the Beluga whale optimization (EBWO) is proposed to deal with the ELD of the large-scale systems. Beluga whale optimization (BWO) is an efficient new optimization technique that mimics the behavior of the Beluga whales (BWs) in preying, swimming, and whale fall. However, the BWO may suffer from stagnation in local optima and scarcity of population diversity like other metaheuristics. The proposed EBWO algorithm is presented to render the standard BWO more robust and powerful search by using two strategies including the cyclone foraging motion for boosting the exploitation phase of the optimization algorithm and the quasi-oppositional based learning (QOBL) for improving population diversity. Firstly, Simulations are carried out on seven benchmark functions to prove the validation of the proposed EBWO algorihm compared with five recent algorithms. Then, The performance of the EBWO is checked on 11-units, 40-units, and also 110-unit test systems, and the obtained results of EBWO are compared with other well-known techniques such as the classical BWO, FOX Optimization Algorithm (FOX), Skill Optimization Algorithm (SOA), and Sand Cat swarm optimization (SCSO) as well as the with existing algorithms from the literature including DE, TLBO, MPSO, NGWO, IGA, NPSO, CJAYA, SMA, PSO, PPSO, SSA, MPA, MGMPA, and HSSA. The Numerical results show that the proposed algorithm is very competitive compared with the other reported optimization algorithms in obtaining low fuel costs.

Poly-herbal therapies for chronic diseases like diabetes mellitus (DM) have been practiced in south Asia for centuries. One of such therapies comprises of Hordeum vulgare, Elettaria cardamomum and Cicer arietinum that have shown encouraging therapeutic potential in the treatment of diabetes and obesity. Therefore, poly-herbal granules (PHGs) of this formula were developed and investigated for their anti-diabetic and anti-obesity potential in obese-diabetic rats. The developed PHGs were chemical characterized and the virtual molecular docking was performed by Discovery studio visualizer (DSV) software. For in-vivo experiment, obesity in rats was induced with high-fat high-sugar diet. After that, diabetes was induced by alloxan monohydrate 150 mg/kg i.p. injection. The diseased rats were treated with PHGs at 250, 500 and 750 mg/kg/day for four weeks. GC-MS analysis of PHGs demonstrated the presence of 1,3-Benzenedicarboxylic acid bis(2-ethylhexyl) ester and 1,2-Benzenedicarboxylic acid di-isooctyl ester and phenol, 2,4-bis(1,1-dimethylethyl). Molecular docking of these compounds demonstrated higher binding energies with receptor than metformin against α-amylase and α-glucosidase. PHGs exhibited a decline in body weight, HbA1c, hyperlipidemia, hyperglycemia, and insulin resistance in diseased rats. The histopathological examination revealed that PHGs improved the alloxan-induced damage to the pancreas. Furthermore, PHGs increased the SOD, CAT and GSH while and the decreased the level of MDA in the liver, kidney and pancreas of diseased rats. Additionally, the PHGs had significantly downregulated the TNF-α and NF-κB while upregulated the expression of NrF-2. The current study demonstrated that the PHGs exhibited anti-diabetic and anti-obesity potential through amelioration of oxidative stress, NF-κB, TNF-α, and NrF-2 due to the presence of different phytochemicals.

Ganoderma lucidum is a versatile mushroom. Polysaccharides and triterpenoids are the major bioactive compounds and have been used as traditional medicinal mushrooms since ancient times. They are currently used as nutraceuticals and functional foods. G. lucidum extracts and their bioactive compounds have been used as an alternative to antioxidants and antimicrobial agents. Secondary metabolites with many medicinal properties make it a possible substitute that could be applied as immunomodulatory, anticancer, antimicrobial, anti-oxidant, anti-inflammatory, and anti-diabetic. The miraculous properties of secondary metabolites fascinate researchers for their development and production. Recent studies have paid close attention to the different physical, genetic, biochemical, and nutritional parameters that potentiate the production of secondary metabolites. This review is an effort to collect biologically active constituents from G. lucidum that reveal potential actions against diseases with the latest improvement in a novel technique to get maximum production of secondary metabolites. Studies are going ahead to determine the efficacy of numerous compounds and assess the valuable properties achieved by G. lucidum in favor of antimicrobial and antioxidant outcomes.

Background: Somalia has long been in a state of humanitarian crisis; trauma-related mental health needs are extremely high. Access to state-of-the-art mental health care is limited. Islamic Trauma Healing (ITH) is a manualized mosque-based, lay-led group intervention aimed at healing the individual and communal mental wounds of war and refugee trauma. The 6-session intervention combines Islamic principles with empirically-supported exposure and cognitive restructuring principles for posttraumatic stress disorder (PTSD). ITH reduces training time, uses a train the trainers (TTT) model, and relies on local partnerships embedded within the strong communal mosque infrastructure. Methods: We will conduct a hybrid effectiveness-implementation randomized control trial (RCT) in the Somaliland, with implementation in the cities of Hargeisa, Borama, and Burao. In this study, a lay-led, mosque-based intervention, Islamic Trauma Healing (ITH), to promote mental health and reconciliation will be examined in 200 participants, randomizing mosques to either immediate ITH or a delayed (waitlist; WL) ITH conditions. Participants will be assessed by assessors masked to condition at pre, 3 weeks, 6 weeks, and 3-month follow-up. Primary outcome will be assessor-rated posttraumatic stress symptoms (PTSD), with secondary outcomes of depression, somatic symptoms, and well-being. A TTT model will be tested, examining the implementation outcomes. Additional measures include potential mechanisms of change and cost effectiveness. Conclusion: This trial has the potential to provide effectiveness and implementation data for an empirically-based principle trauma healing program for the larger Islamic community who may not seek mental health care or does not have access to such care. Clinical trial registration number: ClinicalTrials.gov NCT05890482. World health organization trial registration data set information: See Supplemental Appendix 1.

Brucellosis (undulant fever) is a zoonotic infection caused by Brucella species. It typically presents with fever, malaise, night sweats, and arthralgia. One of its rare complications is infective endocarditis, which occurs in approximately 1.3% of patients and can be further complicated by embolic stroke. This report describes a rare occurrence of Brucella endocarditis presenting as an embolic stroke. A 34-year-old male presented with sudden left-sided weakness and fever. He reported headaches, fever, and generalized weakness in the preceding week. The patient worked on a farm and hence had animal contact. A neurological exam showed left-sided facial weakness, and power of 0/5 and 1/5 in the left upper and lower extremities, respectively. CT scan of the head revealed a right middle cerebral artery (MCA) territory infarct with penumbra and a right MCA occlusion. He underwent a cerebral artery thrombectomy with successful recanalization. However, he continued to have fever and high inflammatory markers. Echocardiography showed aortic valve vegetation and blood cultures grew Brucella melitensis. A multidisciplinary meeting was held to determine the optimal management, which included a course of rifampicin and doxycycline.

Multiple sclerosis (MS) is a demyelinating disorder of the central nervous system (CNS) associated with significant progressive neurodegeneration. There is a lot of interest in the use of plant-based essential oils in traditional medicine to treat and prevent human illnesses, including MS. This research aimed to assess the neuroprotective effects of green tea oil (GTO) and pumpkin oil (PO) against myelin oligodendrocyte glycoprotein (MOG)-induced MS in Wistar rats as well as investigate the underlying molecular mechanisms. The Wistar rats were divided into four groups: group 1, normal control; group 2, MOG-injected; and groups 3 and 4, MOG-injected groups treated with 5 ml/kg body weight each of GTO and PO, respectively. The chemical profiles of components within a GTO and PO were identified using gas chromatography-mass spectrometry (GC–MS). Treatment with GTO and PO substantially improved the decreased dopamine, serotonin, norepinephrine, and acetylcholine levels in the brain of the MOG-injected rats. It also suppressed the elevated epinephrine levels. The histological injuries in the brain cortical tissue of the MOG-injected group were notably improved after supplementing with GTO and PO. Furthermore, brain lipid peroxidation and serum INF-β concentration were significantly lower in the MOG-injected rats treated with GTO and PO. The brain GSH, SOD, GPx, as well as serum coenzyme Q10, and α-tocopherol levels were significantly enhanced by GTO and PO supplementaion. Additionally, GTO and PO administration into MOG-injected rats significantly upregulated Nrf2, Bcl-2, and PCNA while significantly downregulated TNF-α, NF-κB, iNOS, p53, and Bax expression levels. Taken together, these findings suggest that GTO and PO efficiently ameliorate MOG-induced MS via enhancing the antioxidant, anti-inflammatory, and anti-apoptotic effects.

The outburst of the coronavirus into the global arena, first as a respiratory disease and later as a worldwide pandemic and health emergency, pushed the world economic order into complete turmoil and aftermath, posing severe challenges to the financial stability of developing countries like Pakistan. The temporary suspension of economic activities worldwide has resulted in significant disruptions to international supply chains, leading to substantial delays in implementing infrastructure projects associated with the China-Pakistan Economic Corridor (CPEC). The pandemic has further hindered CPEC progress. Building mega-projects, such as the CPEC, is crucial in determining the economic stability of a nation such as Pakistan. Nevertheless, it is essential to consider that the implementation of infrastructure projects can be subject to delays due to the COVID-19 lockdown and travel restrictions. However, it is worth noting that there needs to be more scholarly research available examining the ongoing progress and performance of CPEC projects from a particular perspective. This study aims to assess the impact of the COVID-19 lockdown policy and travel restrictions on CPEC project performances by highlighting the role of socioeconomic and infrastructure development factors. The study will shed light on numerous causes of concerns in project development phases and provide policy recommendations to help CPEC officials reduce project losses and better survival in the event of extreme uncertainty. The data were collected through an online survey from all over Pakistan using self-administered questionnaires with 570 responses from CPEC employees, officials, and professors from management and economic departments. The structural equation modeling (SEM) technique analyzes the problem mentioned above. As per the results of this study, it is evident that the COVID-19 lockdown policy and travel restrictions have a detrimental effect on the construction of the CPEC project. Moreover, it has been observed that the socioeconomic and infrastructure development associated with the CPEC has a notable impact on the performance of the CPEC projects. This paper aims to provide valuable insights to policymakers by examining the management of the COVID-19 pandemic from the perspective of the CPEC.

ABSTRACT: One of the most intriguing areas of research and innovation in the animal production and food sector recently has been designed-enriched products. These items are regarded as functional foods because they feature components that have advantageous physiological impacts on human health. In the production of poultry, designed eggs constitute a significant category of functional foods. The present study hypothesized that adding different kinds of oils to quail diets will help produce designer eggs rich in omega-3 and 6 fatty acids in addition to enhancing productive performance. So, this study examined how linseed (flaxseed) and canola oils with various levels can affect lipid metabolism, immune function, and the amount of n-3 polyunsaturated fatty acids (n-3 PUFA) in Japanese quail eggs. This work was conducted using 3 different vegetable oils (sunflower, linseed, and canola oils) and 3 different antioxidant supplements (0, 250 mg vitamin E/kg feed, and 1,000 mg ginger/kg feed) in a 3 × 3 factorial experiment. When linseed or canola oil was added to the diet, the number of fatty acids in the egg yolks of Japanese quail layers fell by (12.7 and 18.9%) and (41.4 and 24.6%), respectively. The amounts of saturated and monounsaturated fatty acids in total eggs fell by 21.9 and 14.6% and 24.5 and 15.8%, respectively, at 20 wk of age. However, when linseed and canola oil were added to the diet, the sum n-3 PUFA content in the egg yolk of Japanese quail-laying hens was noticeably raised at 15 and 20 wk of age. At 15 and 20 wk of age, the same groups' total n-6 PUFA content considerably increased compared to the group that did not receive flaxseed. In conclusion, during the laying period of Japanese quail, linseed oil, canola oil, vitamin E, or ginger positively affected productivity, blood hematology, constituents, resistance, lipid digestion system, and antioxidative properties in serum and egg yolk.

With the increasing awareness of the serious pollution from the usage of fossil fuels and the fast advances of technologies, the usage of biomass has been increased significantly. In this review paper, the biomass serving as carbon precursors and the energy has been reviewed including lotus lives, wheat flour, fish scale, fish skin, wax gourd, etc. The formed carbon nanostructures are reviewed together with their applications including the treatment volatile organic compounds (VOCs), heavy metal ions from polluted water, spilled oil, fillers for advanced composites, etc. This paper gives the usage of biomass in a sustainable and green way.

Bridge structures adapt to movement through the addition of joint systems that accommodate anticipated movement due to temperature variations or earthquakes, thereby relieving stress on the bridge structure. Shape memory alloys (SMAs) have been proposed to limit joint displacements because of their ability to dissipate seismic energy and restore their original shape. A parametric study is conducted in this study using computer simulations of some advanced MATLAB programs to show the effects of all hysteretic parameters of SMA retrofit devices on seismic joint openings. The results show that SMA hysteretic parameters have a huge effect on SMA efficiency as a restrainer, which is compatible with energy dissipation philosophy, but there are some reversible impacts of some hysteretic parameters in some cases due to the resonance problem and their dependence on other parameters such as natural time period, period ratio, mass ratio, and seismic records. As a result, some design charts are created in this study after more than 200 million trials, taking into account the variation of all key parameters of the structure and the SMA under a suite of historical ground motion records. Moreover, bridge designers can choose optimal SMA hysteresis for a desired opening width, a desired time period, and desired period/mass ratios between adjacent frames with the help of these charts to overcome the two bridge problems of pounding and unseating of bridge decks.

Caffeine (CAF) is a challenging natural bioactive compound with proven antiaging efficacy. However, being hydrophilic hampers its permeation through the skin. Our aim is to develop a novel CAF-loaded nano-cosmeceutical tool counteracting skin photoaging via improving CAF skin permeation using a bioactive nanocarrier. Caffeinated hyalurosomes are novel biocompatible antiaging nanoplatforms designed by immobilization of phospholipid vesicles with a hyaluronan polymer. Physicochemical properties of the selected hyalurosomes formulation showed nano-sized vesicles (210.10 ± 1.87 nm), with high zeta potential (−31.30 ± 1.19 mv), and high encapsulation efficiency (84.60 ± 1.05%). In vitro release results showed outstanding sustained release profile from caffeinated hyalurosomes compared to the CAF-loaded in conventional gel over 24 h. The in-vivo study revealed a photoprotective effect of caffeinated hyalurosomes, reflected from the intact and wrinkling-free skin. Results of biochemical analyses of oxidative stress, pro-inflammatory mediators, and anti-wrinkling markers further confirmed the efficacy of the prepared hyalurosomes compared to the CAF conventional gel. Finally, histopathological examination demonstrated normal histological structures of epidermal layers with minimal inflammatory cell infiltrates in the caffeinated hyalurosomes group compared to the positive control group. Conclusively, caffeinated hyalurosomes successfully achieved enhanced CAF loading and penetration into the skin besides the hydration effect of hyaluronan. Consequently, the developed delivery system presents a promising skin protection nano-platforms via the double effects of both hyaluronan and CAF, hence it guards against skin photodamage.

The production of scaffolds for bone tissue applications is requiring a combination of physical and biological properties, which are depending on the materials morphology and pro-cessing conditions during the production process. The aim of the paper is the investigation of rheological behaviour of polymer and composite blends regularly used for the production of scaffolds for bone tissue applications with the use of additive manufacturing. Poly-ε-caprolactone (PCL), hydroxyapatite (HA), β-tri-calcium phosphate (TCP) and Bioglass 45S5 blends containing different ceramic concentrations (10 wt%, 15 wt% and 20 wt%) were prepared with the use of melt blending procedure and investigated with the use of oscillation and rotational rheology tests. Results are showing that all blends are presenting viscoelastic behaviour with higher viscous modulus, compared with elastic modulus for low frequencies, with this difference reducing while the frequency is increasing. All blends are presenting shear-thinning behaviour suitable for use with additive manufacturing methods. Viscous and elastic modulus are increasing by adding ceramic particles. Results are presenting that PCL/HA blends of the same material concentration are presenting higher elastic modulus properties compared with the other blends, while PCL/Bioglass blends are presenting lower loss factor, lower relaxation time and lower shear viscosity making them easier to handle during the printing procedure.

We studied the effect of Ajuga iva leaves extract (AIE) on the intestinal absorption, motricity and its antioxidant capacity against diarrhea. Wistar rats were divided and received either: castor oil (CO), CO and loperamide or CO and different doses of AIE. AIE prevented dose-dependently CO-induced diarrhea. AIE at 800 mg/kg showed inhibition efficiency on defecation and diarrhea. The pro-oxidant effect of the CO in the small intestine was inhibited significantly in presence of AIE: increasing glutathione peroxidase (GPx) activity and lowering oxygen free radicals (OH°, O2°-), carbonyl protein and malondialdehyde (MDA) levels. However, co-administration of AIE in castor oil-exposed groups significantly increased the intestinal contents of calcium and magnesium. AIE exhibits significant anti-diarrheal activity, related in part to its antioxidant properties. Our investigation also provides experimental evidence for the traditional use of this medicinal plant in the treatment of diarrhea.

Clivia miniata (Lindl) is a member of the family Amaryllidaceae known for its chemically diverse alkaloids with a wide range of biological activities. Many reports revealed a direct role of oxidative stress in the early stage of Alzheimer’s disease (AD). Meanwhile, β-site amyloid precursor protein cleavage enzyme 1 (BACE-1) is a molecular target for the treatment of AD. We aimed to investigate C. miniata root, bulb, and aerial part chemical profiling, antioxidant, BACE-1, and AChE enzyme inhibitory activities. Results showed that the total root had the most potent radical scavenging activity as compared to the total bulb and aerial part, respectively. Ethanol root extract had the most potent BACE-1 inhibitory activity (IC50 = 0.02 ± 0.001 µg/mL) as compared to the bulb and aerial part (IC50 = 0.93 ± 0.13, 1.80 ± 0.24 µg/mL), respectively. Moreover, the total root extract mitigated AChE enzyme activity more than total bulb and aerial fractions with IC50 values of (0.06 ± 0.02, 0.58 ± 0.3, and 1.89 ± 0.42 µg/mL, respectively. Bioassay-guided acid-base fractionation confirmed superior BACE-1 inhibitory activity of the root fractions particularly, methylene chloride and ethyl acetate fractions with (IC50 values of 0.21 ± 0.60 and 0.01 ± 0.001 µg/mL), respectively. UPLC-MS analysis of ethyl acetate and methylene chloride fractions of C. miniata root led to the identification of eight phenolics and thirteen alkaloids, respectively. Molecular docking studies against BACE-1 protein revealed that lycorine di-hexoside, miniatine, and cliviaaline were the most promising hits. Further investigation of anti-AD potential of the aforementioned small molecules is required.

The main objective of this study is to optimize the performance of a wireless sensor network (WSN) in terms of energy consumption and data routing delay using evolutionary metaheuristics. A WSN mobile sink-based routing method in which the sink moves to certain selected rendezvous nodes for data collection is used to address the hot-spot problem. However, there are two challenges, namely, clustering and mobile sink shortest trajectory traversal, which significantly affect the network energy consumption, lifetime, and delay. To achieve the goal of this study, first, a formal model is presented to solve the optimization problem of determining the optimal number of clusters and corresponding cluster heads, which are taken as rendezvous nodes accessed by the mobile sink considering the network energy consumption, intracluster communication, and transmission delay. Second, a discrete differential evolution algorithm is proposed to solve the formulated optimization problem. Third, an ant colony optimization-based algorithm is proposed to construct the shortest path for the mobile sink to traverse the selected rendezvous nodes. The experimental evaluations show that the proposed strategy significantly improves cluster head selection, balances the cluster members, increases the network lifetime by 54%, decreases the transmission delay by 63%, and reduces energy consumption by 47% compared to several routing strategies in the literature.

Summary: Background: Protection against SARS-CoV-2 symptomatic infection and severe COVID-19 of previous infection, mRNA two-dose vaccination, mRNA three-dose vaccination, and hybrid immunity of previous infection and vaccination were investigated in Qatar for the Alpha, Beta, and Delta variants. Methods: Six national, matched, test-negative, case-control studies were conducted between January 18 and December 18, 2021 on a sample of 239,120 PCR-positive tests and 6,103,365 PCR-negative tests. Findings: Effectiveness of previous infection against Alpha, Beta, and Delta reinfection was 89.5% (95% CI: 85.5–92.3%), 87.9% (95% CI: 85.4–89.9%), and 90.0% (95% CI: 86.7–92.5%), respectively. Effectiveness of two-dose BNT162b2 vaccination against Alpha, Beta, and Delta infection was 90.5% (95% CI, 83.9–94.4%), 80.5% (95% CI: 79.0–82.0%), and 58.1% (95% CI: 54.6–61.3%), respectively. Effectiveness of three-dose BNT162b2 vaccination against Delta infection was 91.7% (95% CI: 87.1–94.7%). Effectiveness of hybrid immunity of previous infection and two-dose BNT162b2 vaccination was 97.4% (95% CI: 95.4–98.5%) against Beta infection and 94.5% (95% CI: 92.8–95.8%) against Delta infection. Effectiveness of previous infection and three-dose BNT162b2 vaccination was 98.1% (95% CI: 85.7–99.7%) against Delta infection. All five forms of immunity had >90% protection against severe, critical, or fatal COVID-19 regardless of variant. Similar effectiveness estimates were observed for mRNA-1273. A mathematical model accurately predicted hybrid immunity protection by assuming that the individual effects of previous infection and vaccination acted independently. Interpretation: Hybrid immunity, offering the strongest protection, was mathematically predicted by assuming that the immunities obtained from previous infection and vaccination act independently, without synergy or redundancy. Funding: The Biomedical Research Program and the Biostatistics, Epidemiology, and the Biomathematics Research Core, both at Weill Cornell Medicine-Qatar, Ministry of Public Health, Hamad Medical Corporation, Sidra Medicine, Qatar Genome Programme, Qatar University Biomedical Research Center, and Qatar University Internal Grant ID QUCG-CAS-23/24-114.

Diabetes mellitus is a rapidly spreading global metabolic disorder that has serious social, health, and economic consequences. Herein, we have evaluated in vivo antidiabetic and antihyperlipidemic effects of myrrhanone-B and myrrhanol-B (isolated from Commiphora mukul Hook). We observed that treatment with myrrhanone-B and myrrhanol-B at a dose of 5 and 10 mg/kg body weight for 21 days significantly improved body weight loss, water consumption, and the concentration of blood glucose level (BGL) in alloxan (120 mg/kg) induced diabetic mice, which indicates that the compounds possess strong anti-diabetic activities. In the biochemical analysis, these compounds improved an abnormal level of total cholesterol (TC), triacylglycerol (TG), and low-density lipoprotein cholesterol (LDL-C) to a normal level and increased the high-density lipoprotein cholesterol level (HDLC). Later, drug target of compounds was predicted through in-silico docking which shows that these compounds nicely fit in the active site of α-glucosidase enzyme and mediates excellent interactions with the catalytic residues, Asp214 and Asp349. The in-silico results were confirmed by in-vitro testing of myrrhanone-B and myrrhanol-B against α-glucosidase where both the compounds exhibited excellent inhibitory potency with IC50 values of 19.50 ± 0.71, and 16.11 ± 0.69 µM, respectively. Furthermore, mechanistic study was conducted to observe their binding mechanism, which reflect that myrrhanol-B has mixed type of inhibition (ki = 12.33 ± 0.030 µM), while myrrhanone-B demonstrates competitive type of inhibition (ki =14.53 ± 0.040 µM).

This paper focuses on the impact of three types of roof retrofitting: green, high albedo-coated, also called ‘cool roofs’, and recently developed ‘super cool’ materials—in mitigating the effects of summer urban heat islands (UHI) and enhance overall urban thermal comfort. Human thermal comfort was considered in terms of physiologically equivalent temperature (PET), calculated by combining on-site field measurements with ENVI-met microclimate modelling and the RayMan model. The main environmental parameters addressed were roof surface temperature, ambient air temperature, and mean radiant temperature. The results show that the performance of the super cool roofs surpassed the cool and green roofs at reducing average ambient air and mean radiant temperatures, and the super cool roof had the lowest average PET during the day. However, all the roofs shortened the duration of strong thermal stress by 1 h compared to the conventional roof. This work contributes to the understanding of how roof retrofit technologies can be evaluated at the neighbourhood scale, assisting strategic decisions on building envelope retrofitting for sustainable cities.

Background: Some studies have reported that influenza vaccination is associated with lower risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and/or coronavirus disease 2019 (COVID-19) morbidity and mortality. This study aims to estimate effectiveness of influenza vaccination, using Abbott’s quadrivalent Influvac Tetra vaccine, against SARS-CoV-2 infection and against severe COVID-19. Methods: This matched, test-negative, case-control study was implemented on a population of 30,774 healthcare workers (HCWs) in Qatar during the 2020 annual influenza vaccination campaign, September 17, 2020-December 31, 2020, before introduction of COVID-19 vaccination. Results: Of 30,774 HCWs, 576 with PCR-positive tests and 10,033 with exclusively PCR-negative tests were eligible for inclusion in the study. Matching by sex, age, nationality, reason for PCR testing, and PCR test date yielded 518 cases matched to 2058 controls. Median duration between influenza vaccination and the PCR test was 43 days (IQR, 29–62). Estimated effectiveness of influenza vaccination against SARS-CoV-2 infection> 14 days after receiving the vaccine was 29.7% (95% CI: 5.5–47.7%). Estimated effectiveness of influenza vaccination against severe, critical, or fatal COVID-19 was 88.9% (95% CI: 4.1–98.7%). Sensitivity analyses confirmed the main analysis results. Conclusions: Recent influenza vaccination is associated with a significant reduction in the risk of SARS-CoV-2 infection and COVID-19 severity.

الملخص: أهداف البحث: المحاسبة الاجتماعية هي نهج جديد نسبيا في التعليم الطبي، والمعلومات حول تنفيذه شحيحة في إقليم شرق المتوسط. كان الهدف من مراجعة هذا النطاق هو التحقيق في الأنماط والاتجاهات في الإبلاغ عن المحاسبة الاجتماعية لكليات الطب في منطقة شرق البحر الأبيض المتوسط. طرق البحث: باستخدام نهج أركسي و أومالي، أجريت عمليات بحث في الأدب بين عامي 1995 و 2022 في قواعد البيانات التالية: ''سكوبس'' و ''شبكة العلوم'' و''سينهال'' و ''بابميد'' و ''قوقل العلمي'' باستخدام مصطلحات البحث: المساءلة الاجتماعية، مدرسة الطب، كلية الطب، إقليم شرق المتوسط، منطقة المكتب الإقليمي لشرق المتوسط. باستخدام تحليل الموضوع النوعي، تم الإبلاغ عن البيانات المستخرجة والمشفرة والمحللة. النتائج: كشف فحص قواعد البيانات المختلفة عن 164 مقالا، تم تضمين 26 مقالا أخيرا في المراجعة. المقالات الستة والعشرون المتضمنة نشأت من سبعة فقط من أصل 22 دولة في منطقة الشرق الأوسط: إيران والسودان والمملكة العربية السعودية والإمارات العربية المتحدة ومصر والمغرب وباكستان. تم تصنيف المقالات المدرجة تحت ستة محاور: تعريف المحاسبة الاجتماعية والامتثال لقيم ومفاهيم المحاسبة الاجتماعية، والاعتماد، والبرنامج التعليمي، وتصورات أعضاء هيئة التدريس والطلاب، وقبول الطلاب، وقياس المحاسبة الاجتماعية. الاستنتاجات: كشفت المنشورات المتعلقة بالمساءلة الاجتماعية من دول منطقة شرق المتوسط عن أوجه تشابه مع أجزاء أخرى من العالم من حيث أن مفهومالمحاسبة الاجتماعية لم يتم ترجمته بشكل فعال إلى أنشطة يومية حقيقية في كليات الطب. هناك حاجة إلى مزيد من العمل من القادة في أنظمة التعليم والصحة لفحص عوامل تمكين المحاسبة الاجتماعية وتحسين استيعاب المفهوم. Abstract: Objectives: Social Accountability is a relatively new approach in medical education, Information about its implementation is scarce in the Eastern Mediterranean Region. The objective of this scoping review was to investigate the patterns and trends in reporting the social accountability (SA) of medical schools in the Eastern Mediterranean region (EMR). Methods: Using the Arksey and O'Malley approach, we conducted literature searches between 1995 and 2022 in the following databases: Scopus, Web of Sciences, CINAHL, PubMed, and Google Scholar using the search terms social accountability, medical school, faculty of medicine, Eastern Mediterranean Region, EMRO region. Using qualitative theme analysis, the extracted, coded, and analyzed data were reported. Results: The screening of various databases revealed 164 articles, 26 articles were finally included in the review. The 26 included articles originated from only seven out of the 22 EMR countries: Iran, Sudan, Kingdom of Saudi Arabia (KSA), United Arab Emirates, Egypt, Morocco, and Pakistan. The articles included were categorized under six themes: defining the SA and compliance with SA values and concepts, accreditation, educational program, perceptions of faculty and students, admission of students, and measurement of social accountability. Conclusions: The publications related to SA from the EM regions countries revealed similarities with other parts of the world in that the SA concept is not effectively translated into real day-to-day activities in medical schools. More work is needed from leaders in education and health systems to examine SA enablers and improve the uptake of the concept.

Summary: Quality control of induced pluripotent stem cells remains a challenge. For validation of the pluripotent state, it is crucial to determine trilineage differentiation potential toward endoderm, mesoderm, and ectoderm. Here, we report GermLayerTracker, a combination of site-specific DNA methylation (DNAm) assays that serve as biomarker for early germ layer specification. CG dinucleotides (CpGs) were identified with characteristic DNAm at pluripotent state and after differentiation into endoderm, mesoderm, and ectoderm. Based on this, a pluripotency score was derived that tracks reprogramming and may indicate differentiation capacity, as well as lineage-specific scores to monitor either directed differentiation or self-organized multilineage differentiation in embryoid bodies. Furthermore, we established pyrosequencing assays for fast and cost-effective analysis. In the future, the GermLayerTracker could be used for quality control of pluripotent cells and to estimate lineage-specific commitment during initial differentiation events.