16 results on '"Courcot D"'
Search Results
2. Source apportionment of PM 2.5 oxidative potential in an East Mediterranean site.
- Author
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Fadel M, Courcot D, Delmaire G, Roussel G, Afif C, and Ledoux F
- Abstract
This study aimed to evaluate the oxidative potential (OP) of PM
2.5 collected for almost a year in an urban area of the East Mediterranean. Two acellular assays, based on ascorbic acid (AA) and dithiothreitol (DTT) depletion, were used to measure the OP. The results showed that the mean volume normalized OP-AAv value was 0.64 ± 0.29 nmol·min-1 ·m-3 and the mean OP-DTTv was 0.49 ± 0.26 nmol·min-1 ·m-3 . Several approaches were adopted in this work to study the relationship between the species in PM2.5 (carbonaceous matter, water-soluble ions, major and trace elements, and organic compounds) or their sources and OP values. Spearman correlations revealed strong correlations of OP-AAv with carbonaceous subfractions as well as organic compounds while OP-DTTv seemed to be more correlated with elements emitted from different anthropogenic activities. Furthermore, a multiple linear regression method was used to estimate the contribution of PM2.5 sources, determined by a source-receptor model (Positive Matrix Factorization), to the OP values. The results showed that the sources that highly contribute to the PM2.5 mass (crustal dust and ammonium sulfate) were not the major sources contributing to the values of OP. Instead, 69 % of OP-AAv and 62 % of OP-DTTv values were explained by three local anthropogenic sources: Heavy Fuel Oil (HFO) combustion from a power plant, biomass burning, and road traffic emissions. As for the seasonal variations, higher OP-AAv values were observed during winter compared to summer, while OP-DTTv did not show any significant differences between the two seasons. The contribution of biomass burning during winter was 33 and 34 times higher compared to summer for OP-AAv and OP-DTTv , respectively. On the other hand, higher contributions were observed for HFO combustion during summer., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2023
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3. Oxidative stress response in pulmonary cells exposed to different fractions of PM 2.5-0.3 from urban, traffic and industrial sites.
- Author
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Moufarrej L, Verdin A, Cazier F, Ledoux F, and Courcot D
- Subjects
- Reactive Oxygen Species metabolism, Oxidative Stress, Lung metabolism, 8-Hydroxy-2'-Deoxyguanosine, Particulate Matter analysis, Air Pollutants toxicity, Air Pollutants analysis
- Abstract
The aim of this work was to study the relationship between oxidative stress damages and particulate matter (PM) chemical composition, sources, and PM fractions. PM
2.5-0.3 (PM with equivalent aerodynamic diameter between 2.5 and 0.3 μm) were collected at urban, road traffic and industrial sites in the North of France, and were characterized for major and minor chemical species. Four different fractions (whole PM2.5-0.3 , organic, water-soluble and non-extractable matter) were considered for each of the PM2.5-0.3 samples from the three sites. After exposure of BEAS-2B cells to the four different fractions, oxidative stress was studied in cells by quantifying reactive oxygen species (ROS) accumulation, oxidative damage to proteins (carbonylated proteins), membrane alteration (8-isoprostane) and DNA damages (8-OHdG). Whole PM2.5-0.3 was capable of inducing ROS overproduction and caused damage to proteins at higher levels than other fractions. Stronger cell membrane and DNA damages were found associated with PM and organic fractions from the urban site. ROS overproduction was correlated with level of expression of carbonylated proteins, DNA damages and membrane alteration markers. The PM2.5-0.3 collected under industrial influence appears to be the less linked to cell damages and ROS production in comparison with the other influences., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Inc. All rights reserved.)- Published
- 2023
- Full Text
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4. Chemical profiles of PM 2.5 emitted from various anthropogenic sources of the Eastern Mediterranean: Cooking, wood burning, and diesel generators.
- Author
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Fadel M, Ledoux F, Seigneur M, Oikonomou K, Sciare J, Courcot D, and Afif C
- Subjects
- Aerosols analysis, Animals, Cattle, Cooking, Environmental Monitoring, Water analysis, Wood chemistry, Air Pollutants analysis, Particulate Matter analysis
- Abstract
The chemical profiles of PM
2.5 emitted from a non-road diesel generator, wood burning and cooking activities including chicken and beef charcoal grilling and general cooking activities were determined. The characterization included the carbonaceous fraction (OC/EC), water-soluble ions, elements, and organic species comprising n-alkanes, polycyclic aromatic hydrocarbons, carboxylic acids, levoglucosan, dioxins, furans, and dioxin-like polychlorinated biphenyls. The main component in the PM2.5 from the different sources was carbonaceous matter with a mass contribution to PM2.5 of 49% for cooking activities, 53% for wood burning, 66% for beef grilling, 72% for chicken grilling, and 74% for diesel generator with different OC/EC concentration ratios. The analysis of organic compounds contents using diagnostic ratios and indexes showed differences between the sources and revealed specific source markers. The water-soluble ions had the highest contribution in the cooking activities profile with 17% of PM2.5 and the least in the chicken grilling profile (1.1%). Additionally, 29 analyzed elements were identified, and their contribution varied with the sources (ranging from 1% to 11% of PM2.5 ). These findings could be used to differentiate these sources and could assist in the use of source apportionment methods., (Copyright © 2022 Elsevier Inc. All rights reserved.)- Published
- 2022
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5. Influence of the environmental relative humidity on the inflammatory response of skin model after exposure to various environmental pollutants.
- Author
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Seurat E, Verdin A, Cazier F, Courcot D, Fitoussi R, Vié K, Desauziers V, Momas I, Seta N, and Achard S
- Subjects
- Humans, Humidity, Particulate Matter analysis, Particulate Matter toxicity, Air Pollutants analysis, Air Pollutants toxicity, Environmental Pollutants toxicity, Volatile Organic Compounds analysis, Volatile Organic Compounds toxicity
- Abstract
The skin is an essential barrier, protecting the body against the environment and its numerous pollutants. Several environmental pollutants are known to affect the skin, inducing premature aging through mechanisms including oxidative stress, inflammation, and impairment of skin functions. Even climate conditions can impact the skin. Therefore, using a Reconstructed Human Epidermis (RHE), we tested the effect of two samples of fine particulate matters (PM
0.3-2.5 - one metals-rich sample and the other organic compounds-rich), two Volatile Organic Compounds mixtures (VOCs - from a solvent-based paint and a water-based paint) and Tobacco Smoke (TS). All pollutants affected cellular functionality, but to a lesser extent for the water-based paint VOC. This effect was enhanced when RHE were preconditioned for 2 h by a semi-dry airflow (45% relative humidity) before pollutants application, compared to preconditioning by a humid airflow (90% relative humidity). In the absence of preconditioning, IL-1α, IL-6, IL-8, and RANTES were almost systematically induced by pollutants. When RHE were preconditioned by a semi-dry or humid airflow before being subjected to pollutants, the increase of IL-1α, IL-8, and RANTES falls into two groups. Similarly to RHE not treated with pollutants, RHE treated with VOCs after preconditioning by a semi-dry airflow showed increased IL-1α, IL-8, and RANTES release. On the contrary, RHE treated with PM or TS after preconditioning by a semi-dry airflow show a lower increase in IL-1α, IL-8, and RANTES compared to preconditioning by a humid airflow. The effect of real environmental relative humidity conditions of the air, combined with acute exposure to various environmental pollutants, seemed to relate mainly to structural changes of the skin, determining the outcome of the inflammatory response depending on the physicochemical characteristics of pollutants., (Copyright © 2020 Elsevier Inc. All rights reserved.)- Published
- 2021
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6. Assessment of the PM 2.5 oxidative potential in a coastal industrial city in Northern France: Relationships with chemical composition, local emissions and long range sources.
- Author
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Moufarrej L, Courcot D, and Ledoux F
- Abstract
The objective of this work was to relate PM
2.5 Oxidative Potential (OP) data to PM composition and PM local and distant source contributions. PM2.5 collected in Dunkerque, a coastal industrial city in North of France, was extensively characterized for major and minor chemical species. PM2.5 filters were extracted using a synthetic pulmonary fluid to achieve OP estimation based on Ascorbic Acid (AA) and dithiothreitol (DTT) depletion assays. In order to evidence relationships between OP values, chemical composition and local and distant source contributions, correlation coefficient, Principal Component Analysis (PCA), concentration roses, polar plots and concentration weighted trajectories were used. Heterogeneous conclusions were drawn using the three first methods as the bivariate polar plots lead to dismiss some of the correlations evidenced using correlation coefficient and PCA. Both AA and DTT tests appeared complementary as they were not sensitive to the same species/source contribution. The bivariate polar plot representation of OP values versus wind direction and wind speed revealed that PM2.5 concentration and combustion sources were linked to OP-AA, whereas emissions from integrated steelworks, electric steelworks, heavy fuel oil combustion and traffic non-exhaust significantly contribute to OP-DTT. Sea-salts, aged sea-salts, crustal, secondary sulfates and secondary nitrates sources were not found to contribute to OP values. Constant weighted trajectories evidenced several source regions responsible for high OP values with Belgium, Germany, Netherlands and France at the leader position. Contribution of inland regions appeared possibly related to the biomass and traffic related combustion while heavy fuel oil combustion could also be involved in the contribution of marine and coastal areas., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
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7. An in vitro model to evaluate the impact of environmental fine particles (PM 0.3-2.5 ) on skin damage.
- Author
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Verdin A, Cazier F, Fitoussi R, Blanchet N, Vié K, Courcot D, Momas I, Seta N, and Achard S
- Subjects
- Cell Culture Techniques, Cell Survival, Epidermis metabolism, Gene Expression Regulation drug effects, Humans, Keratinocytes drug effects, Oxidative Stress, Particle Size, Toxicity Tests methods, Air Pollutants toxicity, Epidermis drug effects, Keratinocytes physiology, Particulate Matter toxicity
- Abstract
Exposure to airborne particulate matter (PM) has significant effects on human health mainly leading to cardio-respiratory diseases. However very few data are available regarding the impact of PM on the skin, so to better understand the impact of fine particle (PM
0.3-2.5 ) on both inflammatory response and epidermal structure, we exposed a reconstructed human epidermis (RHE) to several doses of PM collected in Cotonou (Benin, West Africa). After 24 h of exposure, inflammatory response, histological observations, and gene expression related to oxidative stress, antioxidant defense and structural damages were determined. No PM-linked changes in tissue morphology or membrane integrity were observable. PM was however cytotoxic in a dose dependent manner. An inflammatory response appeared as shown by the increase in IL-1α and IL-8 cytokine productions. PM also induced oxidative stress, leading to an increase in 4-HNE immunostaining and to the up-regulation of HMOX1, MT1G and MT1E. Finally, PM had a negative impact on fundamental skin functions such as tissue anchorage, cell differentiation, cornification / skin desquamation and apoptosis. Our data show that airborne fine particles have an adverse effect on skin integrity, most probably leading to accelerated ageing., (Copyright © 2019 Elsevier B.V. All rights reserved.)- Published
- 2019
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8. In vitro evaluation of organic extractable matter from ambient PM 2.5 using human bronchial epithelial BEAS-2B cells: Cytotoxicity, oxidative stress, pro-inflammatory response, genotoxicity, and cell cycle deregulation.
- Author
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Abbas I, Badran G, Verdin A, Ledoux F, Roumie M, Lo Guidice JM, Courcot D, and Garçon G
- Subjects
- Cell Line, DNA Damage, Epithelial Cells, Humans, Oxidative Stress, Air Pollutants toxicity, Cell Cycle drug effects, Particulate Matter toxicity
- Abstract
A particular attention has been devoted to the type of toxicological responses induced by particulate matter (PM), since their knowledge is greatly complicated by the fact that it is a heterogeneous and often poorly described pollutant. However, despite intensive research effort, there is still a lack of knowledge about the specific chemical fraction of PM, which could be mainly responsible of its adverse health effects. We sought also to better investigate the toxicological effects of organic extractable matter (OEM) in normal human bronchial epithelial lung BEAS-2B cells. The wide variety of chemicals, including PAH and other related-chemicals, found in OEM, has been rather associated with early oxidative events, as supported by the early activation of the sensible NRF-2 signaling pathway. For the most harmful conditions, the activation of this signaling pathway could not totally counteract the ROS overproduction, thereby leading to critical oxidative damage to macromolecules (lipid peroxidation, oxidative DNA adducts). While NRF-2 is an anti-inflammatory, OEM exposure did not trigger any significant change in the secretion of inflammatory cytokines (i.e., TNFα, IL-1β, IL-6, IL-8, MCP-1, and IFNγ). According to the high concentrations of PAH and other related organic chemicals found in this OEM, CYP1A1 and 1B1 genes exhibited high transcription levels in BEAS-2B cells, thereby supporting both the activation of the critical AhR signaling pathway and the formation of highly reactive ultimate metabolites. As a consequence, genotoxic events occurred in BEAS-2B cells exposed to this OEM together with cell survival events, with possible harmful cell cycle deregulation. However, more studies are required to implement these observations and to contribute to better decipher the critical role of the organic fraction of air pollution-derived PM
2.5 in the activation of some sensitive signaling pathways closely associated with G1/S and intra-S checkpoint blockage, on the one hand, and cell survival, on the other hand., (Copyright © 2019 Elsevier Inc. All rights reserved.)- Published
- 2019
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9. Usefulness of toxicological validation of VOCs catalytic degradation by air-liquid interface exposure system.
- Author
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Al Zallouha M, Landkocz Y, Brunet J, Cousin R, Genty E, Courcot D, Siffert S, Shirali P, and Billet S
- Subjects
- Environmental Restoration and Remediation instrumentation, Oxidation-Reduction, Volatile Organic Compounds chemistry, Aluminum Oxide chemistry, Catalysis, Environmental Pollutants chemistry, Environmental Restoration and Remediation methods, Palladium chemistry, Toluene chemistry
- Abstract
Toluene is one of the most used Volatile Organic Compounds (VOCs) in the industry despite its major health impacts. Catalytic oxidation represents an efficient remediation technique in order to reduce its emission directly at the source, but it can release by-products. To complete the classical performance assessment using dedicated analytical chemistry methods, we propose to perform an untargeted toxicological validation on two efficient catalysts. Using biological system allows integrating synergy and antagonism in toxic effects of emitted VOCs and by-products, often described in case of multi-exposure condition. Catalysts Pd/α-Al
2 O3 and Pd/γ-Al2 O3 developed for the oxidation of toluene were both coupled to a Vitrocell® Air-Liquid Interface (ALI) system, for exposure of human A549 lung cells during 1h to toluene or to catalysts exhaust before quantification of xenobiotics metabolizing enzymes. This study validated initially the Vitrocell® as an innovative, direct and dynamic model of ALI exposure in the assessment of the performances of new catalysts, showing the presence of chemically undetected by-products. The comparison of the two catalysts showed then that fewer organic compounds metabolizing genes were induced by Pd/γ-Al2 O3 in comparison to Pd/α-Al2 O3 , suggesting that Pd/γ-Al2 O3 is more efficient for toluene total oxidation from a toxicological point of view., (Copyright © 2016. Published by Elsevier Inc.)- Published
- 2017
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10. In vitro short-term exposure to air pollution PM2.5-0.3 induced cell cycle alterations and genetic instability in a human lung cell coculture model.
- Author
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Abbas I, Verdin A, Escande F, Saint-Georges F, Cazier F, Mulliez P, Courcot D, Shirali P, Gosset P, and Garçon G
- Subjects
- Cell Line, Lung drug effects, Particle Size, Air Pollutants toxicity, Apoptosis drug effects, Cell Proliferation drug effects, Gene Expression drug effects, Particulate Matter toxicity, Signal Transduction drug effects
- Abstract
Although its adverse health effects of air pollution particulate matter (PM2.5) are well-documented and often related to oxidative stress and pro-inflammatory response, recent evidence support the role of the remodeling of the airway epithelium involving the regulation of cell death processes. Hence, the overarching goals of the present study were to use an in vitro coculture model, based on human AM and L132 cells to study the possible alteration of TP53-RB gene signaling pathways (i.e. cell cycle phases, gene expression of TP53, BCL2, BAX, P21, CCND1, and RB, and protein concentrations of their active forms), and genetic instability (i.e. LOH and/or MSI) in the PM2.5-0.3-exposed coculture model. PM2.5-0.3 exposure of human AM from the coculture model induced marked cell cycle alterations after 24h, as shown by increased numbers of L132 cells in subG1 and S+G2 cell cycle phases, indicating apoptosis and proliferation. Accordingly, activation of the TP53-RB gene signaling pathways after the coculture model exposure to PM2.5-0.3 was reported in the L132 cells. Exposure of human AM from the coculture model to PM2.5-0.3 resulted in MS alterations in 3p chromosome multiple critical regions in L132 cell population. Hence, in vitro short-term exposure of the coculture model to PM2.5-0.3 induced cell cycle alterations relying on the sequential occurrence of molecular abnormalities from TP53-RB gene signaling pathway activation and genetic instability., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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11. Temporal-spatial variations of the physicochemical characteristics of air pollution Particulate Matter (PM2.5-0.3) and toxicological effects in human bronchial epithelial cells (BEAS-2B).
- Author
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Dergham M, Lepers C, Verdin A, Cazier F, Billet S, Courcot D, Shirali P, and Garçon G
- Subjects
- Cell Line, Environmental Monitoring, Epithelial Cells drug effects, Humans, Multivariate Analysis, Particle Size, Principal Component Analysis, Reactive Oxygen Species metabolism, Air Pollutants toxicity, Particulate Matter toxicity, Respiratory Mucosa drug effects
- Abstract
While the evidence for the health adverse effects of air pollution Particulate Matter (PM) has been growing, there is still uncertainty as to which constituents within PM are most harmful. Hence, to contribute to fulfill this gap of knowledge, some physicochemical characteristics and toxicological endpoints (i.e. cytotoxicity, oxidative damage, cytokine secretion) of PM2.5-0.3 samples produced during two different seasons (i.e. spring/summer or autumn/winter) in three different surroundings (i.e. rural, urban, or industrial) were studied, thereby expecting to differentiate their respective adverse effects in human bronchial epithelial cells (BEAS-2B). Physicochemical characteristics were closely related to respective origins and seasons of the six PM2.5-0.3 samples, highlighting the respective contributions of industrial and heavy motor vehicle traffic sources. Space- and season-dependent differences in cytotoxicity of the six PM2.5-0.3 samples could only be supported by considering both the physicochemical properties and the variance in air PM concentrations. Whatever spaces and seasons, dose- and even time-dependent increases in oxidative damage and cytokine secretion were reported in PM2.5-0.3-exposed BEAS-2B cells. However, the relationship between the chemical composition of each of the six PM2.5-0.3 samples and their oxidative or inflammatory potentials seemed to be very complex. These results supported the role of inorganic, ionic and organic components as exogenous source of Reactive Oxygen Species and, thereafter, cytokine secretion. Nevertheless, one of the most striking observation was that some inorganic, ionic and organic chemical components were preferentially associated with early oxidative events whereas others in the later oxidative damage and/or cytokine secretion. Taken together, these results indicated that PM mass concentration alone might not be able to explain the health outcomes, because PM is chemically nonspecific, and supported growing evidence that PM-size, composition and emission source, together with sampling season, interact in a complex manner to produce PM2.5-0.3-induced human adverse health effects., (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Published
- 2015
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12. Genotoxic and epigenotoxic effects of fine particulate matter from rural and urban sites in Lebanon on human bronchial epithelial cells.
- Author
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Borgie M, Ledoux F, Verdin A, Cazier F, Greige H, Shirali P, Courcot D, and Dagher Z
- Subjects
- Bronchi cytology, Bronchi metabolism, Cell Line, Epithelial Cells drug effects, Epithelial Cells metabolism, Histones metabolism, Humans, Lebanon, Microscopy, Electron, Scanning, Phosphorylation, Bronchi drug effects, Mutagens toxicity, Particulate Matter toxicity
- Abstract
Assessment of air pollution by particulate matter (PM) is strongly required in Lebanon in the absence of an air quality law including updated air quality standards. Using two different PM2.5-0.3 samples collected at an urban and a rural site, we examined genotoxic/epigenotoxic effects of PM exposure within a human bronchial epithelial cell line (BEAS-2B). Inorganic and organic contents evidence the major contribution of traffic and generating sets in the PM2.5-0.3 composition. Urban PM2.5-0.3 sample increased the phosphorylation of H2AX, the telomerase activity and the miR-21 up-regulation in BEAS-2B cells in a dose-dependent manner. Furthermore, urban PM2.5-0.3 induced a significant increase in CYP1A1, CYP1B1 and AhRR genes expression. The variable concentrations of transition metals and organic compounds detected in the collected PM2.5-0.3 samples might be the active agents leading to a cumulative DNA damage, critical for carcinogenesis., (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Published
- 2015
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13. Chemical profile identification of fugitive and confined particle emissions from an integrated iron and steelmaking plant.
- Author
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Hleis D, Fernández-Olmo I, Ledoux F, Kfoury A, Courcot L, Desmonts T, and Courcot D
- Subjects
- Air Pollution, Anions, Cations, Microscopy, Electron, Scanning, Particle Size, Thermogravimetry, X-Ray Diffraction, Air Pollutants isolation & purification, Dust, Environmental Monitoring methods, Iron chemistry, Steel chemistry
- Abstract
The aim of this study is to obtain the characteristic inorganic chemical profile of important particle sources identified in the integrated iron and steel process: sintering, blast furnace, steelmaking and desulfurization slag processing. A complete chemical and physical characterization program was developed: particle size distribution, chemical analysis, XRD, SEM-EDX and TGA/DTA. The sample collected from the sinter stack showed high levels of K and Cl(-), followed by Fe, NH4(+), Ca, Na and Pb. The profile of the dust samples taken from the sinter cake discharge zone was quite different, showing higher amounts of Fe, Ca and Al, and lower amounts of K, Cl(-), Na and Pb. Dust samples collected from the blast furnace (BF) and steelmaking cast house may be distinguished from each other based on the higher levels of Fe (hematite and magnetite) and lower levels of Ca, Zn and C (graphite) found in BF dust. High levels of Ca and Fe were found in samples taken from the desulfurization slag processing area. Such information can be useful for source apportionment studies at receptor sites that could be influenced by iron and steelmaking plant emissions., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
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14. Relationship between physicochemical characterization and toxicity of fine particulate matter (PM2.5) collected in Dakar city (Senegal).
- Author
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Dieme D, Cabral-Ndior M, Garçon G, Verdin A, Billet S, Cazier F, Courcot D, Diouf A, and Shirali P
- Subjects
- Air Pollution analysis, Cell Line, Cities, Dose-Response Relationship, Drug, Epithelial Cells metabolism, Glutathione metabolism, Humans, Inflammation chemically induced, Inflammation metabolism, Oxidative Stress, Particulate Matter analysis, Particulate Matter chemistry, Polycyclic Aromatic Hydrocarbons metabolism, Respiratory Mucosa cytology, Senegal, Volatile Organic Compounds metabolism, Air Pollution adverse effects, Bronchi cytology, Epithelial Cells drug effects, Particulate Matter toxicity
- Abstract
The massive increase in emissions of air pollutants due to economic and industrial growth in developing countries has made air quality a crucial health problem in this continent. Hence, it is somewhat critical to have a better knowledge on the air pollution in Sub-Saharan Africa countries. Three air pollution PM2.5 samples were also collected in two urban sites (i.e., Fann and Faidherbe) in Dakar (Senegal) and in a rural site near Dakar (i.e., Ngaparu). The two urban sites mainly differ in the type of used vehicles: in Fann, most of the traffic is made of buses, which are absent, in Faidherbe. The physicochemical characteristics of the three PM2.5 samples revealed their high heterogeneities and complexities, related to the multiple natural and anthropogenic emission sources. Results from 5-bromodeoxyuridine incorporation into DNA, mitochondrial dehydrogenase activity, and extracellular lactate dehydrogenase activity in PM2.5-exposed BEAS-2B cells suggested the exposure conditions (i.e., 3 and 12 μg PM/cm² during 24, 48, and 72 h) to further consider. The organic fractions (i.e., mainly PAHs) of the PM(2.5) samples were able to induce a time and/or concentration-dependent gene expression of CYP1A1 and CYP1B1, and, to a lesser extent, NQO1. There was a time and/or dose-dependent increase of both the gene expression and/or protein secretion of inflammatory mediators (i.e., TNF-α, IL-1β, IL-6, and/or IL-8) in PM(2.5)-exposed BEAS-2B cells. In agreement with the physicochemical characterization, urban PM(2.5) samples caused greater biological responses in BEAS-2B cells than the rural one. Variable concentrations of transition metals (i.e., Fe, Al, Pb, Mn, Zn) and organic compounds (i.e., PAHs) founded in the three PM2.5 samples might be firmly involved in a time- and/or dose-dependent toxicity, relying on inflammatory processes., (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Published
- 2012
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15. Ambient particulate matter (PM2.5): physicochemical characterization and metabolic activation of the organic fraction in human lung epithelial cells (A549).
- Author
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Billet S, Garçon G, Dagher Z, Verdin A, Ledoux F, Cazier F, Courcot D, Aboukais A, and Shirali P
- Subjects
- Cell Line, Cell Survival drug effects, Cytochrome P-450 Enzyme System genetics, Epithelial Cells cytology, Epithelial Cells metabolism, Epoxide Hydrolases genetics, France, Glutathione Transferase genetics, Humans, Lung cytology, NAD(P)H Dehydrogenase (Quinone) genetics, Organic Chemicals analysis, Particle Size, RNA, Messenger metabolism, Air Pollutants analysis, Air Pollutants toxicity, Epithelial Cells drug effects, Gene Expression Regulation, Enzymologic drug effects, Particulate Matter analysis, Particulate Matter toxicity
- Abstract
To contribute to complete the knowledge of the underlying mechanisms of action involved in air pollution particulate matter (PM)-induced cytotoxicity, an aerosol was collected in Dunkerque, a French seaside City heavily industrialized. In this work, we focused our attention on its physical and chemical characteristics, its cytotoxicity, and its role in the induction of the volatile organic compound (VOC) and/or polycyclic aromatic hydrocarbon (PAH)-metabolizing enzymes in human lung epithelial cells (A549). Size distribution showed that 92.15% of the collected PM were PM2.5 and the specific surface area was 1 m2/g. Inorganic (i.e. Fe, Al, Ca, Na, K, Mg, Pb, etc.) and organic (i.e. VOC, PAH, etc.) chemicals were found in collected PM, revealing that much of them derived from wind-borne dust from the industrial complex and the heavy motor vehicle traffic. The thermal desorption study indicated that organic chemicals were not only adsorbed onto the surface but also highly incrusted in the structure of PM. The lethal concentrations at 10% and 50% of collected PM were 23.72 microg/mL (or 6.33microg/cm2) and 118.60 microg/mL (or 31.63 microg/cm2), respectively. The VOC and/or PAH-coated onto PM induced significant increases in mRNA expressions of cytochrome P450 (cyp) 1a1, cyp2e1, cyp2f1, nadph quinone oxydo-reductase-1, and glutathione s-transferase-pi 1, versus controls. Hence, we concluded that the metabolic activation of the very low doses of VOC and/or PAH-coated onto the inorganic condensation nuclei from Dunkerque City's PM is one of the underlying mechanisms of action closely involved in its cytotoxicity in human lung epithelial cells.
- Published
- 2007
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16. Activation of different pathways of apoptosis by air pollution particulate matter (PM2.5) in human epithelial lung cells (L132) in culture.
- Author
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Dagher Z, Garçon G, Billet S, Gosset P, Ledoux F, Courcot D, Aboukais A, and Shirali P
- Subjects
- 8-Hydroxy-2'-Deoxyguanosine, Air Pollutants analysis, Air Pollution analysis, Caspases metabolism, Cell Line, Cytochromes c metabolism, DNA Fragmentation drug effects, Deoxyguanosine analogs & derivatives, Deoxyguanosine metabolism, Epithelial Cells cytology, Epithelial Cells drug effects, Epithelial Cells metabolism, France, Gene Expression Regulation drug effects, Humans, Lung cytology, Lung drug effects, Lung metabolism, Metals analysis, Metals toxicity, Mitochondria drug effects, Mitochondria metabolism, Particle Size, Polycyclic Aromatic Hydrocarbons analysis, Polycyclic Aromatic Hydrocarbons toxicity, Proto-Oncogene Proteins c-bcl-2 genetics, Proto-Oncogene Proteins c-bcl-2 metabolism, RNA, Messenger metabolism, Tumor Necrosis Factor-alpha biosynthesis, Tumor Suppressor Protein p53 genetics, Tumor Suppressor Protein p53 metabolism, bcl-2-Associated X Protein genetics, bcl-2-Associated X Protein metabolism, Air Pollutants toxicity, Air Pollution adverse effects, Apoptosis drug effects
- Abstract
Epidemiological studies have associated the increase of respiratory and cardiovascular mortality and morbidity with high levels of air pollution particulate matter (PM). However, the underlying mechanisms of actions by which PM induce adverse health effects are still unclear. We have recently undertaken an extensive investigation of the adverse health effects of air pollution PM(2.5), and shown that in vitro short-term exposure to PM(2.5) induced oxidative stress and inflammation in human lung epithelial cells (L132). Hence, it was convenient to complete the physical and chemical characterization of PM and to investigate whether in vitro short-term exposure to PM could be imply in the activation of apoptosis. Accordingly, we found that 92.15% of PM were equal or smaller than 2.5 microm and their specific surface area was 1m(2)/g. Inorganic (i.e. Fe, Al, Ca, Na, K, Mg, Pb, etc.) and organic (i.e. polycyclic aromatic hydrocarbons) chemicals were found in PM, suggesting that much of them derived from wind-borne dust from the industrial complex and the heavy motor vehicle traffic. In other respects, we showed that PM exposure induced apoptosis by activating not only the tumor necrosis factor-alpha (TNF-alpha)-induced pathway (i.e. TNF-alpha secretion, caspase-8 and -3 activation), but also the mitochondrial pathway (i.e. 8-hydroxy-2'-desoxyguanosine formation, cytochrome c release from mitochondria, caspase-9 and -3 activation). Moreover, changes in the transcription rates of p53, bcl-2, and bax genes, on the one hand, and DNA fragmentation, on the other hand, were reported in PM-exposed proliferating L132 cells, revealing the occurrence of apoptotic events. Taken together, these findings suggested that in vitro short-term exposure to PM(2.5) induced apoptosis in L132 cells.
- Published
- 2006
- Full Text
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