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An in vitro model to evaluate the impact of environmental fine particles (PM 0.3-2.5 ) on skin damage.
- Source :
-
Toxicology letters [Toxicol Lett] 2019 May 01; Vol. 305, pp. 94-102. Date of Electronic Publication: 2019 Feb 01. - Publication Year :
- 2019
-
Abstract
- Exposure to airborne particulate matter (PM) has significant effects on human health mainly leading to cardio-respiratory diseases. However very few data are available regarding the impact of PM on the skin, so to better understand the impact of fine particle (PM <subscript>0.3-2.5</subscript> ) on both inflammatory response and epidermal structure, we exposed a reconstructed human epidermis (RHE) to several doses of PM collected in Cotonou (Benin, West Africa). After 24 h of exposure, inflammatory response, histological observations, and gene expression related to oxidative stress, antioxidant defense and structural damages were determined. No PM-linked changes in tissue morphology or membrane integrity were observable. PM was however cytotoxic in a dose dependent manner. An inflammatory response appeared as shown by the increase in IL-1α and IL-8 cytokine productions. PM also induced oxidative stress, leading to an increase in 4-HNE immunostaining and to the up-regulation of HMOX1, MT1G and MT1E. Finally, PM had a negative impact on fundamental skin functions such as tissue anchorage, cell differentiation, cornification / skin desquamation and apoptosis. Our data show that airborne fine particles have an adverse effect on skin integrity, most probably leading to accelerated ageing.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1879-3169
- Volume :
- 305
- Database :
- MEDLINE
- Journal :
- Toxicology letters
- Publication Type :
- Academic Journal
- Accession number :
- 30716388
- Full Text :
- https://doi.org/10.1016/j.toxlet.2019.01.016