128 results on '"Drosten, Christian"'
Search Results
2. Divergent Genotype of Hepatitis A Virus in Alpacas, Bolivia, 2019.
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Veith, Talitha, Beltran-Saavedra, L. Fabian, Bleicker, Tobias, Schmidt, Marie Luisa, Mollericona, José L., Grützmacher, Kim, Wallace, Rob, Drexler, Jan Felix, Walzer, Christian, Jones, Terry C., Drosten, Christian, and Corman, Victor Max
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Hepatitis A virus (HAV) is a common human pathogen found exclusively in primates. In a molecular and serologic study of 64 alpacas in Bolivia, we detected RNA of distinct HAV in ≈9% of animals and HAV anti-bodies in ≈64%. Complete-genome analysis suggests a long association of HAV with alpacas. [ABSTRACT FROM AUTHOR]
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- 2023
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3. Effectiveness of Naturally Acquired and Vaccine-Induced Immune Responses to SARS-CoV-2 Mu Variant.
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de Oliveira-Filho, Edmilson F., Rincon-Orozco, Bladimiro, Jones-Cifuentes, Natalia, Peña-López, Brigitte, Mühlemann, Barbara, Drosten, Christian, Moreira-Soto, Andres, and Drexler, Jan Felix
- Abstract
SARS-CoV-2 Mu variant emerged in Colombia in 2021 and spread globally. In 49 serum samples from vaccinees and COVID-19 survivors in Colombia, neutralization was significantly lower (p<0.0001) for Mu than a parental strain and variants of concern. Only the Omicron variant of concern demonstrated higher immune evasion. [ABSTRACT FROM AUTHOR]
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- 2022
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4. Emergence of SARS-CoV-2 Delta Variant, Benin, May-July 2021.
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Yadouleton, Anges, Sander, Anna-Lena, Adewumi, Praise, de Oliveira Filho, Edmilson F., Tchibozo, Carine, Hounkanrin, Gildas, René, Keke K., Ange, Dossou, Kohoun, Rodrigue K., Nari, Ramalia Chabi, Salifou, Sourakatou, Saizonou, Raoul, Kakai, Clement G., Bedié, Sonia V., Al Onifade, Fattah, Nagel, Michael, Joël Aïssi, Melchior A., Akogbeto, Petas, Drosten, Christian, and Wulf, Ben
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SARS-CoV-2 ,COVID-19 ,VACCINATION coverage ,DISEASE incidence - Abstract
Severe acute respiratory syndrome coronavirus 2 Delta variant epidemiology in Africa is unknown. We found Delta variant was introduced in Benin during April-May 2021 and became predominant within 2 months, after which a steep increase in reported coronavirus disease incidence occurred. Benin might require increased nonpharmaceutical interventions and vaccination coverage. [ABSTRACT FROM AUTHOR]
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- 2022
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5. Mutations Associated with SARS-CoV-2 Variants of Concern, Benin, Early 2021.
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Sander, Anna-Lena, Yadouleton, Anges, de Oliveira Filho, Edmilson F., Tchibozo, Carine, Hounkanrin, Gildas, Badou, Yvette, Adewumi, Praise, René, Keke K., Ange, Dossou, Sourakatou, Salifou, Sedjro, Eclou, Aïssi, Melchior A. Joël, Fidelia, Hinson, Djingarey, Mamoudou Harouna, Nagel, Michael, Jo, Wendy Karen, Moreira-Soto, Andres, Drosten, Christian, Landt, Olfert, and Corman, Victor Max
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SARS-CoV-2 ,COVID-19 - Abstract
Intense transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Africa might promote emergence of variants. We describe 10 SARS-CoV-2 lineages in Benin during early 2021 that harbored mutations associated with variants of concern. Benin-derived SARS-CoV-2 strains were more efficiently neutralized by antibodies derived from vaccinees than patients, warranting accelerated vaccination in Africa. [ABSTRACT FROM AUTHOR]
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- 2021
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6. Outbreak of SARS-CoV-2 B.1.1.7 Lineage after Vaccination in Long-Term Care Facility, Germany, February-March 2021.
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Tober-Lau, Pinkus, Schwarz, Tatjana, Hillus, David, Spieckermann, Jana, Helbig, Elisa T., Lippert, Lena J., Thibeault, Charlotte, Koch, Willi, Bergfeld, Leon, Niemeyer, Daniela, Mühlemann, Barbara, Conrad, Claudia, Kasper, Stefanie, Münn, Friederike, Kunitz, Frank, Jones, Terry C., Suttorp, Norbert, Drosten, Christian, Sander, Leif Erik, and Kurth, Florian
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LONG-term care facilities ,SARS-CoV-2 ,VACCINATION - Abstract
One week after second vaccinations were administered, an outbreak of B.1.1.7 lineage severe acute respiratory syndrome coronavirus 2 infections occurred in a long-term care facility in Berlin, Germany, affecting 16/20 vaccinated and 4/4 unvaccinated residents. Despite considerable viral loads, vaccinated residents experienced mild symptoms and faster time to negative test results. [ABSTRACT FROM AUTHOR]
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- 2021
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7. Severe Acute Respiratory Syndrome Coronavirus 2 Outbreak Related to a Nightclub, Germany, 2020.
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Muller, Nadine, Kunze, Mareike, Steitz, Fabienne, Saad, Neil J., Mühlemann, Barbara, Beheim-Schwarzbach, Jörn I., Schneider, Julia, Drosten, Christian, Murajda, Lukas, Kochs, Sandra, Ruscher, Claudia, Walter, Jan, Zeitlmann, Nadine, and Corman, Victor M.
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COVID-19 ,VIRAL transmission ,EPIDEMICS ,SUPERSPREADING events ,NIGHTCLUBS - Abstract
We report an outbreak of coronavirus disease with 74 cases related to a nightclub in Germany in March 2020. Staff members were particularly affected (attack rate 56%) and likely caused sustained viral transmission after an event at the club. This outbreak illustrates the potential for superspreader events and corroborates current club closures. [ABSTRACT FROM AUTHOR]
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- 2021
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8. Hepatitis E Virus Genotype 7 RNA and Antibody Kinetics in Naturally Infected Dromedary Calves, United Arab Emirates.
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Corman, Victor M., Nagy, Peter, Ostermann, Stefanie, Arloth, Jacqueline, Liljander, Anne, Barua, Rajib, Das Gupta, Aungshuman, Hakimuddin, Fatima, Juhasz, Judit, Wernery, Ulrich, and Drosten, Christian
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HEPATITIS E virus ,CAMELS ,CALVES ,GENOTYPES ,ANALYTICAL mechanics ,RESEARCH ,CATTLE ,ANIMAL experimentation ,RESEARCH methodology ,HEPATITIS viruses ,HEPATITIS E ,RNA ,MEDICAL cooperation ,EVALUATION research ,COMPARATIVE studies - Abstract
Orthohepevirus A genotype 7 is a novel zoonotic variant of hepatitis E virus. To clarify infection in the animal reservoir, we virologically monitored 11 dromedary dam-calf pairs. All calves became infected during the first 6 months of life and cleared the virus after an average of 2 months. Dams did not become infected. [ABSTRACT FROM AUTHOR]
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- 2020
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9. Severe Acute Respiratory Syndrome Coronavirus 2-Specific Antibody Responses in Coronavirus Disease Patients.
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Okba, Nisreen M. A., Müller, Marcel A., Wentao Li, Chunyan Wang, GeurtsvanKessel, Corine H., Corman, Victor M., Lamers, Mart M., Sikkema, Reina S., de Bruin, Erwin, Chandler, Felicity D., Yazdanpanah, Yazdan, Le Hingrat, Quentin, Descamps, Diane, Houhou-Fidouh, Nadhira, Reusken, Chantal B. E. M., Bosch, Berend-Jan, Drosten, Christian, Koopmans, Marion P. G., Haagmans, Bart L., and Li, Wentao
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VIRAL pneumonia ,CLINICAL pathology ,SERODIAGNOSIS ,COVID-19 ,EPIDEMICS ,NEUTRALIZATION tests ,ENZYME-linked immunosorbent assay ,VIRAL antibodies - Abstract
A new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has recently emerged to cause a human pandemic. Although molecular diagnostic tests were rapidly developed, serologic assays are still lacking, yet urgently needed. Validated serologic assays are needed for contact tracing, identifying the viral reservoir, and epidemiologic studies. We developed serologic assays for detection of SARS-CoV-2 neutralizing, spike protein-specific, and nucleocapsid-specific antibodies. Using serum samples from patients with PCR-confirmed SARS-CoV-2 infections, other coronaviruses, or other respiratory pathogenic infections, we validated and tested various antigens in different in-house and commercial ELISAs. We demonstrated that most PCR-confirmed SARS-CoV-2-infected persons seroconverted by 2 weeks after disease onset. We found that commercial S1 IgG or IgA ELISAs were of lower specificity, and sensitivity varied between the 2 assays; the IgA ELISA showed higher sensitivity. Overall, the validated assays described can be instrumental for detection of SARS-CoV-2-specific antibodies for diagnostic, seroepidemiologic, and vaccine evaluation studies. [ABSTRACT FROM AUTHOR]
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- 2020
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10. Inactivation of Severe Acute Respiratory Syndrome Coronavirus 2 by WHO-Recommended Hand Rub Formulations and Alcohols.
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Kratzel, Annika, Todt, Daniel, V'kovski, Philip, Steiner, Silvio, Gultom, Mitra, Tran Thi Nhu Thao, Ebert, Nadine, Holwerda, Melle, Steinmann, Jörg, Niemeyer, Daniela, Dijkman, Ronald, Kampf, Günter, Drosten, Christian, Steinmann, Eike, Thiel, Volker, Pfaender, Stephanie, and Thao, Tran Thi Nhu
- Abstract
Infection control instructions call for use of alcohol-based hand rub solutions to inactivate severe acute respiratory syndrome coronavirus 2. We determined the virucidal activity of World Health Organization-recommended hand rub formulations, at full strength and multiple dilutions, and of the active ingredients. All disinfectants demonstrated efficient virus inactivation. [ABSTRACT FROM AUTHOR]
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- 2020
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11. Severe Acute Respiratory Syndrome Coronavirus 2 Outbreak Related to a Nightclub, Germany, 2020.
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Muller, Nadine, Kunze, Mareike, Steitz, Fabienne, Saad, Neil J, Mühlemann, Barbara, Beheim-Schwarzbach, Jörn I, Schneider, Julia, Drosten, Christian, Murajda, Lukas, Kochs, Sandra, Ruscher, Claudia, Walter, Jan, Zeitlmann, Nadine, and Corman, Victor M
- Abstract
We report an outbreak of coronavirus disease with 74 cases related to a nightclub in Germany in March 2020. Staff members were particularly affected (attack rate 56%) and likely caused sustained viral transmission after an event at the club. This outbreak illustrates the potential for superspreader events and corroborates current club closures. [ABSTRACT FROM AUTHOR]
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- 2020
- Full Text
- View/download PDF
12. Comparison of Serologic Assays for Middle East Respiratory Syndrome Coronavirus.
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Harvey, Ruth, Mattiuzzo, Giada, Hassall, Mark, Sieberg, Andrea, Müller, Marcel A., Drosten, Christian, Rigsby, Peter, Oxenford, Christopher J., and study participants
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COMPARATIVE studies ,CORONAVIRUS diseases ,ENZYME-linked immunosorbent assay ,FLUORESCENT antibody technique ,RESEARCH methodology ,MEDICAL cooperation ,RESEARCH ,RESEARCH evaluation ,SERODIAGNOSIS ,VIRAL antibodies ,EVALUATION research ,OLIGONUCLEOTIDE arrays ,NEUTRALIZATION tests ,MERS coronavirus - Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) was detected in humans in 2012. Since then, sporadic outbreaks with primary transmission through dromedary camels to humans and outbreaks in healthcare settings have shown that MERS-CoV continues to pose a threat to human health. Several serologic assays for MERS-CoV have been developed globally. We describe a collaborative study to investigate the comparability of serologic assays for MERS-CoV and assess any benefit associated with the introduction of a standard reference reagent for MERS-CoV serology. Our study findings indicate that, when possible, laboratories should use a testing algorithm including >2 tests to ensure correct diagnosis of MERS-CoV. We also demonstrate that the use of a reference reagent greatly improves the agreement between assays, enabling more consistent and therefore more meaningful comparisons between results. [ABSTRACT FROM AUTHOR]
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- 2019
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13. Sand Fly-Associated Phlebovirus with Evidence of Neutralizing Antibodies in Humans, Kenya.
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Tchouassi, David P., Marklewitz, Marco, Chepkorir, Edith, Zirkel, Florian, Agha, Sheila B., Tigoi, Caroline C., Koskei, Edith, Drosten, Christian, Borgemeister, Christian, Torto, Baldwyn, Junglen, Sandra, and Sang, Rosemary
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HUMAN beings ,IMMUNOGLOBULINS - Abstract
We describe a novel virus, designated Ntepes virus (NPV), isolated from sand flies in Kenya. NPV has the characteristic phlebovirus trisegmented genome architecture and is related to, but distinct from, Gabek Forest phlebovirus. Diverse cell cultures derived from wildlife, livestock, and humans were susceptible to NPV, with pronounced permissiveness in swine and rodent cells. NPV infection of newborn mice caused rapid and fatal illness. Permissiveness for NPV replication in sand fly cells, but not mosquito cells, suggests a vector-specific adaptation. Specific neutralizing antibodies were found in 13.9% (26/187) of human serum samples taken at the site of isolation of NPV as well as a disparate site in northeastern Kenya, suggesting a wide distribution. We identify a novel human-infecting arbovirus and highlight the importance of rural areas in tropical Africa for arbovirus surveillance as well as extending arbovirus surveillance to include hematophagous arthropods other than mosquitoes. [ABSTRACT FROM AUTHOR]
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- 2019
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14. Differential Shedding and Antibody Kinetics of Zika and Chikungunya Viruses, Brazil.
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Bozza, Fernando A., Moreira-Soto, Andres, Rockstroh, Alexandra, Fischer, Carlo, Nascimento, Alessandra D., Calheiros, Andrea S., Drosten, Christian, Bozza, Patrícia T., Souza, Thiago Moreno L., Ulbert, Sebastian, and Drexler, Jan Felix
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CHIKUNGUNYA virus ,ZIKA virus - Abstract
In seroconversion panels obtained from patients from Brazil, diagnostic testing for Zika virus infection was improved by combining multiple antibody isotypes, techniques, and antigens, but sensitivity remained suboptimal. In contrast, chikungunya virus diagnostic testing was unambiguous. Recurrent recent arbovirus infections suggested by serologic data and unspecific symptoms highlight the need for exhaustive virologic testing. [ABSTRACT FROM AUTHOR]
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- 2019
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15. Lineage-Specific Real-Time RT-PCR for Yellow Fever Virus Outbreak Surveillance, Brazil.
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Nogueira, Rita Maria Ribeiro, Bispo de Filippis, Ana Maria, Kümmerer, Beate M., Fischer, Carlo, Moreira-Soto, Andres, Drexler, Jan Felix, Torres, Maria C., Ribeiro Nogueira, Rita Maria, Sequeira, Patricia C., Rodrigues, Cintia D. S., de Filippis, Ana Maria Bispo, Patel, Pranav, Landt, Olfert, Drosten, Christian, Gould, Ernest A., Charrel, Rémi N., and de Lamballerie, Xavier
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YELLOW fever ,REVERSE transcriptase polymerase chain reaction ,YELLOW fever vaccines ,DISEASE outbreaks ,COMPARATIVE studies ,FLAVIVIRUSES ,IMMUNITY ,RESEARCH methodology ,MEDICAL cooperation ,POLYMERASE chain reaction ,RESEARCH ,EVALUATION research - Abstract
The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease. We developed novel multiplex real-time reverse transcription PCRs that differentiate between vaccine and American wild-type YFV. We validated these highly specific and sensitive assays in an outbreak setting. [ABSTRACT FROM AUTHOR]
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- 2017
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16. Hepatitis E Virus Infection in European Brown Hares, Germany, 2007-2014.
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Corman, Victor Max, Hilgensloh, Laura, Voigt, Ulrich, Marklewitz, Marco, Siebert, Ursula, Drosten, Christian, and Drexler, Jan Felix
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HEPATITIS E virus ,VIRUS diseases ,HARES ,HEPATITIS viruses - Abstract
Rabbit-associated hepatitis E viruses (HEVs) cause zoonotic infections. We investigated 2,389 hares in Germany during 2007-2014. Complete genome characterization of a hare-associated HEV strain revealed close genomic relatedness to rabbit-associated HEV strains. Although hare-specific HEV seroprevalence was low, at 2.6%, hares represent a potential source of sporadic HEV infections. [ABSTRACT FROM AUTHOR]
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- 2019
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17. Hepatitis E Virus Infection in Dromedaries, North and East Africa, United Arab Emirates, and Pakistan, 1983-2015.
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Rasche, Andrea, Saqib, Muhammad, Liljander, Anne M., Bornstein, Set, Zohaib, Ali, Renneker, Stefanie, Steinhagen, Katja, Wernery, Renate, Younan, Mario, Gluecks, Ilona, Hilali, Mosaad, Musa, Bakri E., Jores, Joerg, Wernery, Ulrich, Drexer, Jan Felix, Drosten, Christian, Corman, Victor Max, and Drexler, Jan Felix
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CAMEL diseases ,HEPATITIS E virus ,HEPATITIS E ,ZOONOSES - Abstract
A new hepatitis E virus (HEV-7) was recently found in dromedaries and 1 human from the United Arab Emirates. We screened 2,438 dromedary samples from Pakistan, the United Arab Emirates, and 4 African countries. HEV-7 is long established, diversified and geographically widespread. Dromedaries may constitute a neglected source of zoonotic HEV infections. [ABSTRACT FROM AUTHOR]
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- 2016
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18. MERS-CoV Antibodies in Humans, Africa, 2013-2014.
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Liljander, Anne, Meyer, Benjamin, Jores, Joerg, Müller, Marcel A., Lattwein, Erik, Njeru, Ian, Bett, Bernard, Drosten, Christian, and Corman, Victor Max
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MERS coronavirus ,VIRAL antibodies ,MIDDLE East respiratory syndrome ,MIDDLE East respiratory syndrome transmission ,PUBLIC health ,PREVENTION - Abstract
Dromedaries in Africa and elsewhere carry the Middle East respiratory syndrome coronavirus (MERS-CoV). To search for evidence of autochthonous MERS-CoV infection in humans, we tested archived serum from livestock handlers in Kenya for MERS-CoV antibodies. Serologic evidence of infection was confirmed for 2 persons sampled in 2013 and 2014. [ABSTRACT FROM AUTHOR]
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- 2016
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19. No Evidence of Gouléako and Herbert Virus Infections in Pigs, Côte d'Ivoire and Ghana.
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Junglen, Sandra, Marklewitz, Marco, Zirkel, Florian, Wollny, Robert, Meyer, Benjamin, Heidemann, Hanna, Metzger, Sonja, Annan, Augustina, Dei, Dickson, Leendertz, Fabian H, Oppong, Samuel, and Drosten, Christian
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VETERINARY virology ,BUNYAVIRUSES ,SWINE diseases ,CELL culture ,BLOOD serum analysis ,ANIMALS ,MOSQUITOES ,RNA viruses ,SWINE ,RNA virus infections - Abstract
A recent report suggested that 2 novel bunyaviruses discovered in insects in Côte d'Ivoire caused lethal disease in swine in South Korea. We conducted cell culture studies and tested serum from pigs exposed to mosquitoes in Côte d'Ivoire and Ghana and found no evidence for infection in pigs. [ABSTRACT FROM AUTHOR]
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- 2015
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20. Occupational Exposure to Dromedaries and Risk for MERS-CoV Infection, Qatar, 2013-2014.
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Reusken, Chantal B.E.M., Farag, Elmoubasher A.B.A., Haagmans, Bart L., Mohran, Khaled A., Godeke, Gert-Jan, Raj, V. Stalin, Alhajri, Farhoud, Al-Marri, Salih A., Al-Romaihi, Hamad E., Al-Thani, Mohamed, Bosch, Berend-Jan, van der Eijk, Annemiek A., El-Sayed, Ahmed M., Ibrahim, Adel K., Al-Molawi, N., Müller, Marcel A., Pasha, Syed K., Drosten, Christian, AlHajri, Mohd M., and Koopmans, Marion P.G.
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MIDDLE East respiratory syndrome transmission ,CAMELS as carriers of disease ,IMMUNOGLOBULIN G ,OCCUPATIONAL diseases ,BLOOD serum analysis ,IMMUNE system - Abstract
We determined the presence of neutralizing antibodies to Middle East respiratory syndrome Coronavirus in persons in Qatar with and without dromedary contact. Antibodies were only detected in those with contact, suggesting dromedary exposure as a risk factor for infection. Findings also showed evidence for substantial underestimation of the infection in populations at risk in Qatar. [ABSTRACT FROM AUTHOR]
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- 2015
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21. Human-Bat Interactions in Rural West Africa.
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Anti, Priscilla, Owusu, Michael, Agbenyega, Olivia, Annan, Augustina, Badu, Ebenezer Kofi, Nkrumah, Evans Ewald, Tschapka, Marco, Oppong, Samuel, Adu-Sarkodie, Yaw, and Drosten, Christian
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VIRAL disease diagnosis ,BATS as carriers of disease ,PUBLIC health ,EBOLA virus ,RURAL population ,MEDICAL care - Abstract
Because some bats host viruses with zoonotic potential, we investigated human-bat interactions in rural Ghana during 2011-2012. Nearly half (46.6%) of respondents regularly visited bat caves; 37.4% had been bitten, scratched, or exposed to bat urine; and 45.6% ate bat meat. Human-bat interactions in rural Ghana are frequent and diverse. [ABSTRACT FROM AUTHOR]
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- 2015
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22. Novel Arenavirus Isolates from Namaqua Rock Mice, Namibia, Southern Africa.
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Witkowski, Peter T., Kallies, René, Hoveka, Julia, Auste, Brita, Ithete, Ndapewa L., Šoltys, Katarína, Szemes, Tomáš, Drosten, Christian, Preiser, Wolfgang, Klempa, Boris, Mfune, John K.E., and Kruger, Detlev H.
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ARENAVIRUS diseases ,ARENAVIRUSES ,MOUSE diseases ,VETERINARY virology ,VETERINARY epidemiology - Abstract
Arenaviruses are feared as agents that cause viral hemorrhagic fevers. We report the identification, isolation, and genetic characterization of 2 novel arenaviruses from Namaqua rock mice in Namibia. These findings extend knowledge of the distribution and diversity of arenaviruses in Africa. [ABSTRACT FROM AUTHOR]
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- 2015
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23. Acute Middle East Respiratory Syndrome Coronavirus Infection in Livestock Dromedaries, Dubai, 2014.
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Wernery, Ulrich, Corman, Victor M., Wong, Emily Y. M., Tsang, Alan K. L., Muth, Doreen, Lau, Susanna K. P., Khazanehdari, Kamal, Zirkel, Florian, Ali, Mansoor, Nagy, Peter, Juhasz, Jutka, Wernery, Renate, Joseph, Sunitha, Syriac, Ginu, Elizabeth, Shyna K., Patteril, Nissy Annie Georgy, Woo, Patrick C. Y., and Drosten, Christian
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CORONAVIRUS diseases ,RNA ,VIRUS diseases ,IMMUNOGLOBULINS ,BLOOD proteins - Abstract
Camels carry Middle East respiratory syndrome coronavirus, but little is known about infection age or prevalence. We studied >800 dromedaries of all ages and 15 mother-calf pairs. This syndrome constitutes an acute, epidemic, and time-limited infection in camels <4 years of age, particularly calves. Delayed social separation of calves might reduce human infection risk. [ABSTRACT FROM AUTHOR]
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- 2015
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24. Time Course of MERS-CoV Infection and Immunity in Dromedary Camels.
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Meyer, Benjamin, Juhasz, Judit, Barua, Rajib, Das Gupta, Aungshuman, Hakimuddin, Fatima, Corman, Victor M., Müller, Marcel A., Wernery, Ulrich, Drosten, Christian, and Nagy, Peter
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MIDDLE East respiratory syndrome transmission ,MIDDLE East respiratory syndrome ,ANIMAL vaccination ,MICROBIAL sensitivity tests ,CAMEL diseases ,DIAGNOSIS ,ANIMAL experimentation ,ANIMAL diseases ,CATTLE ,COMPARATIVE studies ,CORONAVIRUS diseases ,IMMUNOGLOBULINS ,LONGITUDINAL method ,RESEARCH methodology ,MEDICAL cooperation ,RESEARCH ,RNA ,VIRAL antibodies ,ZOONOSES ,EVALUATION research ,MERS coronavirus ,INFECTIOUS disease transmission - Abstract
Knowledge about immunity to Middle East respiratory syndrome coronavirus (MERS-CoV) in dromedary camels is essential for infection control and vaccination. A longitudinal study of 11 dam-calf pairs showed that calves lose maternal MERS-CoV antibodies 5-6 months postparturition and are left susceptible to infection, indicating a short window of opportunity for vaccination. [ABSTRACT FROM AUTHOR]
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- 2016
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25. Antibodies against MERS coronavirus in dromedary camels, Kenya, 1992-2013.
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Corman, Victor M., Jores, Joerg, Meyer, Benjamin, Younan, Mario, Liljander, Anne, Said, Mohammed Y., Gluecks, Ilona, Lattwein, Erik, Bosch, Berend-Jan, Drexler, Jan Felix, Bornstein, Set, Drosten, Christian, and Müller, Marcel A.
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MIDDLE East respiratory syndrome in animals ,CAMEL diseases ,CORONAVIRUS diseases ,CORONAVIRUSES ,MERS coronavirus ,ANIMAL diseases ,ANIMAL experimentation ,ENZYME-linked immunosorbent assay ,MAMMALS ,POPULATION density ,POPULATION geography ,VIRAL antibodies ,INFECTIOUS disease transmission - Abstract
Dromedary camels are a putative source for human infections with Middle East respiratory syndrome coronavirus. We showed that camels sampled in different regions in Kenya during 1992-2013 have antibodies against this virus. High densities of camel populations correlated with increased seropositivity and might be a factor in predicting long-term virus maintenance. [ABSTRACT FROM AUTHOR]
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- 2014
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26. Human Infection with MERS Coronavirus after Exposure to Infected Camels, Saudi Arabia, 2013.
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Memish, Ziad A., Cotten, Matthew, Meyer, Benjamin, Watson, Simon J., Alsahafi, Abdullah J., Al Rabeeah, Abdullah A., Corman, Victor Max, Sieberg, Andrea, Makhdoom, Hatem Q., Assiri, Abdullah, Al Masri, Malaki, Aldabbagh, Souhaib, Bosch, Berend-Jan, Beer, Martin, Müller, Marcel A., Kellam, Paul, and Drosten, Christian
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MERS coronavirus ,MIDDLE East respiratory syndrome ,CAMEL diseases ,DISEASE vectors ,NUCLEOTIDES ,GENETIC polymorphism research - Abstract
We investigated a case of human infection with Middle East respiratory syndrome coronavirus (MERS-CoV) after exposure to infected camels. Analysis of the whole human-derived virus and 15% of the camel-derived virus sequence yielded nucleotide polymorphism signatures suggestive of cross-species transmission. Camels may act as a direct source of human MERS-CoV infection. [ABSTRACT FROM AUTHOR]
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- 2014
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27. Antibodies against MERS coronavirus in dromedary camels, United Arab Emirates, 2003 and 2013.
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Meyer, Benjamin, Müller, Marcel A, Corman, Victor M, Reusken, Chantal B E M, Ritz, Daniel, Godeke, Gert-Jan, Lattwein, Erik, Kallies, Stephan, Siemens, Artem, van Beek, Janko, Drexler, Jan F, Muth, Doreen, Bosch, Berend-Jan, Wernery, Ulrich, Koopmans, Marion P G, Wernery, Renate, and Drosten, Christian
- Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) has caused an ongoing outbreak of severe acute respiratory tract infection in humans in the Arabian Peninsula since 2012. Dromedary camels have been implicated as possible viral reservoirs. We used serologic assays to analyze 651 dromedary camel serum samples from the United Arab Emirates; 151 of 651 samples were obtained in 2003, well before onset of the current epidemic, and 500 serum samples were obtained in 2013. Recombinant spike protein-specific immunofluorescence and virus neutralization tests enabled clear discrimination between MERS-CoV and bovine CoV infections. Most (632/651, 97.1%) camels had antibodies against MERS-CoV. This result included all 151 serum samples obtained in 2003. Most (389/651, 59.8%) serum samples had MERS-CoV-neutralizing antibody titers >1,280. Dromedary camels from the United Arab Emirates were infected at high rates with MERS-CoV or a closely related, probably conspecific, virus long before the first human MERS cases. [ABSTRACT FROM AUTHOR]
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- 2014
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28. Contact Investigation for Imported Case of Middle East Respiratory Syndrome, Germany.
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Böhmer, Merle, Reuss, Annicka, Litterst, Annette, Drosten, Christian, Seilmaier, Michael, Graf, Petra, Gold, Hermann, Wendtner, Clemens-Martin, Zanuzdana, Arina, Schaade, Lars, Haas, Walter, and Buchholz, Udo
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RESPIRATORY infections ,INFECTION ,RESPIRATORY diseases ,CORONAVIRUS diseases ,MIDDLE East respiratory syndrome ,CORONAVIRUSES ,INFECTIOUS disease transmission - Abstract
On March 19, 2013, a patient from United Arab Emirates who had severe respiratory infection was transferred to a hospital in Germany, 11 days after symptom onset. Infection with Middle East respiratory syndrome coronavirus (MERS-CoV) was suspected on March 21 and confirmed on March 23; the patient, who had contact with an ill camel shortly before symptom onset, died on March 26. A contact investigation was initiated to identify possible person-to-person transmission and assess infection control measures. Of 83 identified contacts, 81 were available for follow-up. Ten contacts experienced mild symptoms, but test results for respiratory and serum samples were negative for MERS-CoV. Serologic testing was done for 53 (75%) of 71 nonsymptomatic contacts; all results were negative. Among contacts, the use of FFP2/FFP3 face masks during aerosol exposure was more frequent after MERS-CoV infection was suspected than before. Infection control measures may have prevented nosocomial transmission of the virus. [ABSTRACT FROM AUTHOR]
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- 2014
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29. Antibodies against MERS Coronavirus in Dromedaries, United Arab Emirates, 2003 and 2013.
- Author
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Meyer, Benjamin, Müller, Marcel A., Corman, Victor M., Reusken, Chantal B. E. M., Ritz, Daniel, Godeke, Gert-Jan, Lattwein, Erik, Kallies, Stephan, Siemens, Artem, van Beek, Janko, Drexler, Jan F., Muth, Doreen, Bosch, Berend-Jan, Wernery, Ulrich, Koopmans, Marion P. G., Wernery, Renate, and Drosten, Christian
- Subjects
CORONAVIRUSES ,CORONAVIRUS diseases ,RESPIRATORY diseases ,RESPIRATORY infections ,IMMUNOFLUORESCENCE ,VIRUS diseases ,CAMEL diseases - Abstract
Middle East respiratory syndrome coronavirus (MERSCoV) has caused an ongoing outbreak of severe acute respiratory tract infection in humans in the Arabian Peninsula since 2012. Dromedaries have been implicated as possible viral reservoirs. We used serologic assays to analyze 651 dromedary serum samples from the United Arab Emirates; 151 of 651 samples were obtained in 2003, well before onset of the current epidemic, and 500 serum samples were obtained in 2013. Recombinant spike protein-specific immunofluorescence and virus neutralization tests enabled clear discrimination between MERS-CoV and bovine CoV infections. Most (632/651, 97.1%) dromedaries had antibodies against MERS-CoV. This result included all 151 serum samples obtained in 2003. Most (389/651, 59.8%) serum samples had MERS-CoV neutralizing antibody titers >1,280. Dromedaries from the United Arab Emirates were infected at high rates with MERS-CoV or a closely related, probably conspecific, virus long before the first human MERS cases. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
30. Replicative Capacity of MERS Coronavirus in Livestock Cell Lines.
- Author
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Eckerle, Isabella, Corman, Victor M., Müller, Marcel A., Lenk, Matthias, Ulrich, Rainer G., and Drosten, Christian
- Subjects
MERS coronavirus ,MIDDLE East respiratory syndrome ,LIVESTOCK diseases ,CELL lines ,GOAT diseases ,CAMEL diseases - Abstract
Replicative capacity of Middle East respiratory syndrome coronavirus (MERS-CoV) was assessed in cell lines derived from livestock and peridomestic small mammals on the Arabian Peninsula. Only cell lines originating from goats and camels showed efficient replication of MERSCoV. These results provide direction in the search for the intermediate host of MERS-CoV. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
31. Early Introduction and Delayed Dissemination of Pandemic influenza, Gabon.
- Author
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Lekana-Douki, Sonia Etenna, Mouinga-Ondémé, Augustin, Nkoghe, Dieudonné, Drosten, Christian, Drexler, Jan Felix, Kazanji, Mirdad, and Leroy, Eric M.
- Subjects
H1N1 influenza ,COUGH - Abstract
Active surveillance in health care centers in Gabon during 2009-2011 detected 72 clinical cases of pandemic (H1N1) 2009 (pH1N1). We found that pH1N1 virus was introduced in mid-2009 but spread throughout the country in 2010. Thus, Gabon was also affected by pH1N1. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
32. Human Parvovirus 4 in Nasal and Fecal Specimens from Children, Ghana.
- Author
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Drexler, Jan Felix, Reber, Ulrike, Muth, Doreen, Herzog, Petra, Annan, Augustina, Ebach, Fabian, Sarpong, Nimarko, Acquah, Samuel, Adlkofer, Julia, Adu-Sarkodie, Yaw, Panning, Marcus, Tannich, Egbert, May, Jürgen, Drosten, Christian, and Eis-Hübinger, Anna Maria
- Subjects
PARVOVIRUS diseases ,DNA ,SAMPLING (Process) ,MEDICAL virology ,INFECTIOUS disease transmission - Abstract
Nonparenteral transmission might contribute to human parvovirus 4 (PARV4) infections in sub-Saharan Africa. PARV4 DNA was detected in 8 (0.83%) of 961 nasal samples and 5 (0.53%) of 943 fecal samples from 1,904 children in Ghana. Virus concentrations ≈6-7 log[sub 10] copies/mL suggest respiratory or fecal-oral modes of PARV4 transmission. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
33. Cosavirus Infection in Persons with and without Gastroenteritis, Brazil.
- Author
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Stöcker, Andreas, Souza, Breno Frederico de Carvalho Dominguez, Ribeiro, Tereza Cristina Medrado, Netto, Eduardo Martins, Araujo, Luciana Oliveira, Corrêa, Jefferson Ivan, Almeida, Patrícia Silva, de Mattos, Angela Peixoto, Ribeiro Jr., Hugo da Costa, Pedral-Sampaio, Diana Brasil, Drosten, Christian, and Drexler, Jan Felix
- Subjects
JUVENILE diseases ,GASTROENTERITIS ,REVERSE transcriptase polymerase chain reaction ,PATHOGENIC microorganisms ,DIARRHEA ,RNA viruses - Abstract
The article discusses a study that investigated the prevalence of cosavirus in children and HIV-infected adults with gastroenteritis and control subjects without gastroenteritis in Brazil. Real-time reverse transcription polymerase chain reaction (PCR) was used to test the study subjects. Co-infections with common viral pathogens causing diarrhea were analyzed to determine whether cosavirus causes disease in sick children. Evidence against cosavirus involvement in human gastroenteritis include unusually low RNA virus concentrations and the higher prevalence of cosavirus found in controls than in patients.
- Published
- 2012
- Full Text
- View/download PDF
34. Aichi virus shedding in high concentrations in patients with acute diarrhea.
- Author
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Drexler JF, Baumgarte S, de Souza Luna LK, Eschbach-Bludau M, Lukashev AN, Drosten C, Drexler, Jan Felix, Baumgarte, Sigrid, de Souza Luna, Luciano Kleber, Eschbach-Bludau, Monika, Lukashev, Alexander N, and Drosten, Christian
- Abstract
We assessed Aichi virus shedding in patients with gastroenteritis and negative test results for other viral and bacterial infections. High concentrations of up to 1.32 × 1012 RNA copies/g stool were found in 10 (2.0%) of 499 outpatients sampled in northern Germany, 2004. These data substantiate Aichi virus pathogenicity in humans. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
35. Amplification of Emerging Viruses in a Bat Colony.
- Author
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Drexler, Jan Felix, Corman, Victor Max, Wegner, Tom, Tateno, Adriana Fumie, Zerbinati, Rodrigo Melim, Gloza-Rausch, Florian, Seebens, Antje, Müller, Marcel A., and Drosten, Christian
- Subjects
BATS ,CORONAVIRUSES ,NIDOVIRUSES ,RNA viruses ,ADENOVIRUSES ,PATHOGENIC microorganisms - Abstract
Bats host noteworthy viral pathogens, including coronaviruses, astroviruses, and adenoviruses. Knowledge on the ecology of reservoir-borne viruses is critical for preventive approaches against zoonotic epidemics. We studied a maternity colony of Myotis myotis bats in the attic of a private house in a suburban neighborhood in Rhineland-Palatinate, Germany, during 2008, 2009, and 2010. One coronavirus, 6 astroviruses, and 1 novel adenovirus were identified and monitored quantitatively. Strong and specific amplification of RNA viruses, but not of DNA viruses, occurred during colony formation and after parturition. The breeding success of the colony was significantly better in 2010 than in 2008, in spite of stronger amplification of coronaviruses and astroviruses in 2010, suggesting that these viruses had little pathogenic influence on bats. However, the general correlation of virus and bat population dynamics suggests that bats control infections similar to other mammals and that they may well experience epidemics of viruses under certain circumstances. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
36. Novel human parvovirus 4 genotype 3 in infants, Ghana.
- Author
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Panning M, Kobbe R, Vollbach S, Drexler JF, Adjei S, Adjei O, Drosten C, May J, Eis-Hubinger AM, Panning, Marcus, Kobbe, Robin, Vollbach, Silke, Drexler, Jan Felix, Adjei, Samuel, Adjei, Ohene, Drosten, Christian, May, Jürgen, and Eis-Hubinger, Anna Maria
- Abstract
Human parvovirus 4 has been considered to be transmitted only parenterally. However, after novel genotype 3 of parvovirus 4 was found in 2 patients with no parenteral risks, we tested infants in Ghana. A viremia rate of 8.6% over 2 years indicates that this infection is common in children in Africa. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
37. Distant Relatives of Severe Acute Respiratory Syndrome Coronavirus and Close Relatives of Human Coronavirus 229E in Bats, Ghana.
- Author
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Pfefferle, Susanne, Oppong, Samuel, Drexler, Jan Felix, Gloza-Rausch, Florian, Ipsen, Anne, Seebens, Antje, Müller, Marcel A., Annan, Augustina, Vallo, Peter, Adu-Sarkodie, Yaw, Kruppa, Thomas F., and Drosten, Christian
- Subjects
SARS disease ,CORONAVIRUSES ,BAT diseases ,RESPIRATORY infections ,RESPIRATORY diseases - Abstract
We tested 12 bat species in Ghana for coronavirus (CoV) RNA. The virus prevalence in insectivorous bats (n = 123) was 9.76%. CoV was not detected in 212 fecal samples from Eidolon helvum fruit bats. Leaf-nosed bats pertaining to Hipposideros ruber by morphology had group 1 and group 2 CoVs. Virus concentrations were ≤45,000 copies/100 mg of bat feces. The diversified group 1 CoV shared a common ancestor with the human common cold virus hCoV-229E but not with hCoV-NL63, disputing hypotheses of common human descent. The most recent common ancestor of hCoV-229E and GhanaBt-CoVGrp1 existed in ≈1686-1800 AD. The GhanaBt-CoVGrp2 shared an old ancestor (≈2,400 years) with the severe acute respiratory syndrome-like group of CoV. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
38. Novel Human Parechovirus from Brazil.
- Author
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Drexler, Jan Felix, Grywna, Klaus, Stöcker, Andreas, Almeida, Patrícia Silva, Medrado Ribeiro, Tereza Cristina, Eschbach-Bludau, Monika, Petersen, Nadine, Ribeiro Jr., Hugo da Costa, and Drosten, Christian
- Subjects
REVERSE transcriptase ,POLYMERASE chain reaction ,JUVENILE diseases ,ENTERITIS - Abstract
Human parechoviruses (HPeVs) were detected by reverse transcription--PCR in 16.1% of 335 stool samples from children <6 years of age with enteritis in Salvador, Brazil. Whole genome sequencing of 1 sample showed a novel HPeV that has been designated as HPeV8. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
39. Circulation of 3 Lineages of a Novel Saffold Cardiovirus in Humans.
- Author
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Drexler, Jan Felix, De Souza Luna, Luciano Kleber, Stöcker, Andreas, Almeida, Patrícia Silva, Ribeiro, Tereza Cristina Medrado, Petersen, Nadine, Herzog, Petra, Pedroso, Célia, Huppertz, Hans Iko, Ribeiro Jr., Hugo da Costa, Baumgarte, Sigrid, and Drosten, Christian
- Subjects
VIRUSES ,CARDIOVASCULAR diseases ,MOLECULAR diagnosis ,MULTIPLE sclerosis ,MYOCARDITIS ,ENCEPHALOMYELITIS ,DIABETES - Abstract
Cardioviruses cause serious disease, mainly in rodents, including diabetes, myocarditis, encephalomyelitis, and multiple sclerosis--like disseminated encephalomyelitis. Recently, a human virus isolate obtained 25 years ago, termed Saffold virus, was sequenced and classified as a cardiovirus. We conducted systematic molecular screening for Saffold-like viruses in 844 fecal samples from patients with gastroenteritis from Germany and Brazil, across all age groups. Six cardioviruses were identified in patients <6 years of age. Viral loads were 283,305-5,044,412,175 copies/g of stool. Co-infections occurred in 4 of 6 children. No evidence for outbreak-like epidemic patterns was found. Phylogenetic analysis identified 3 distinct genetic lineages. Viral protein 1 amino acids were 67.9%-77.7% identical and had a distance of at least 39.4% from known cardioviruses. Because closely related strains were found on 2 continents, global distribution in humans is suspected. Saffold- like viruses may be the first human cardiovirus species to be identified. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
40. Chikungunya Fever in Travelers Returning to Europe from the Indian Ocean Region, 2006.
- Author
-
Panning, Marcus, Grywna, Klaus, Van Esbroeck, Marjan, Emmerich, Petra, and Drosten, Christian
- Subjects
CHIKUNGUNYA ,FEVER ,TOGAVIRUS infections ,TRAVELERS ,IMMUNOFLUORESCENCE ,IMMUNOGLOBULIN M ,IMMUNOGLOBULIN G - Abstract
Chikungunya fever has spread through several Indian Ocean islands and India, including popular travel destinations. To compare usefulness of diagnostic tests and to understand reasons for the magnitude and severity of an outbreak, we used 3 diagnostic methods to test 720 samples from 680 patients returning to Europe from the Indian Ocean region in 2006. Chikungunya infection was confirmed for 24.4% patients in the first half of the year and for 9.9% in the second half. Reverse transcription-PCR was positive for all samples taken up to day 4 after symptom onset. Immunofl uorescence detected immunoglobulin (Ig) M on day 1 and IgG on day 2 for some patients, and in all patients from day 5 onward. Soon after onset of symptoms, patients had IgG and IgM and high viral loads (some >109 copies/mL plasma). These data will help healthcare providers select diagnostic tests for returning travelers. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
41. Viral load as predictor of Crimean-Congo hemorrhagic fever outcome.
- Author
-
Duh, Darja, Saksida, Ana, Petrovec, Miroslav, Ahmeti, Salih, Dedushaj, Iusuf, Panning, Marcus, Drosten, Christian, Avšič-Županc, Tatjana, and Avsic-Zupanc, Tatjana
- Subjects
HEMORRHAGIC fever ,POLYMERASE chain reaction ,VIRUSES ,PATIENTS ,IMMUNOGLOBULINS - Abstract
We used quantitative real-time reverse transcription-PCR to measure viral load in serum from 24 patients in Kosovo who had acute Crimean-Congo hemorrhagic fever. Viral load correlated with clinical disease and antibodies and could be used as a predictor of disease outcome. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
42. Coronavirus antibodies in African bat species.
- Author
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Müller, Marcel A., Paweska, Janusz T., Leman, Patricia A., Drosten, Christian, Grywna, Klaus, Kemp, Alan, Braack, Leo, Sonnenberg, Karen, Niedrig, Matthias, Swanepoel, Robert, and Müller, Marcel A
- Subjects
BATS ,CORONAVIRUSES ,SARS disease ,IMMUNOGLOBULINS ,ANTIGENS ,SERUM - Abstract
Asian bats have been identified as potential reservoir hosts of coronaviruses associated with severe acute respiratory syndrome (SARS-CoV). We detected antibody reactive with SARS-CoV antigen in 47 (6.7%) of 705 bat serum specimens comprising 26 species collected in Africa; thus, African bats may harbor agents related to putative group 4 CoV. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
43. Virus detection and monitoring of viral load in Crimean-Congo hemorrhagic fever virus patients.
- Author
-
Wölfel, Roman, Paweska, Janusz T., Petersen, Nadine, Grobbelaar, Antoinette A., Leman, Patricia A., Hewson, Roger, Georges-Courbot, Marie-Claude, Papa, Anna, Günther, Stephan, Drosten, Christian, Wölfel, Roman, and Günther, Stephan
- Subjects
HEMORRHAGIC fever ,POLYMERASE chain reaction ,VIRUS diseases ,SERUM ,VIRUSES ,INFECTION ,DIAGNOSIS ,PATIENTS ,REVERSE transcriptase polymerase chain reaction ,RESEARCH ,GENETICS ,RESEARCH evaluation ,BIOLOGICAL evolution ,VIRAL load ,RESEARCH methodology ,MEDICAL cooperation ,EVALUATION research ,COMPARATIVE studies ,IMMUNITY ,RNA viruses ,STANDARDS - Abstract
We developed a real-time reverse transcription--PCR that detected 1,164 copies/mL of Crimean-Congo hemorrhagic fever virus per milliliter of serum at 95% probability (probit analysis) and was 100% concordant with nested PCR on 63 samples from 31 patients with confirmed infection. Infected patients who died appeared to have higher viral loads; low viral loads correlated with IgG detection. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
44. MassTag polymerase chain reaction for differential diagnosis of viral hemorrhagic fever.
- Author
-
Palacios, Gustavo, Briese, Thomas, Kapoor, Vishal, Jabado, Omar, Zhiqiang Liu, Venter, Marietjie, Junhui Zhai, Renwick, Neil, Grolla, Allen, Geisbert, Thomas W., Drosten, Christian, Towner, Jonathan, Jingyue Ju, Paweska, Janusz, Nichol, Stuart T., Swanepoel, Robert, Feldmann, Heinz, Jahrling, Peter B., Lipkin, W. Ian, and Liu, Zhiqiang
- Subjects
VIRUS diseases ,COMMUNICABLE diseases ,FEVER ,ETIOLOGY of diseases ,POLYMERASE chain reaction ,PHYSICAL & theoretical chemistry ,COMPARATIVE studies ,COMPUTER software ,DIFFERENTIAL diagnosis ,HEMORRHAGIC fever ,RESEARCH methodology ,MEDICAL cooperation ,RESEARCH ,RESEARCH funding ,EVALUATION research ,DIAGNOSIS - Abstract
Viral hemorrhagic fevers are associated with high rates of illness and death. Although therapeutic options are limited, early differential diagnosis has implications for containment and may aid in clinical management. We describe a diagnostic system for rapid, multiplex polymerase chain reaction identification of 10 different causes of viral hemorrhagic fevers. [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
- View/download PDF
45. SARS Molecular Detection External Quality Assurance.
- Author
-
Drosten, Christian, Doerr, Hans Wilhelm, Lim, Wilina, Stöhr, Klaus, and Niedrig, Matthias
- Subjects
- *
RESPIRATORY infections , *SARS disease , *CORONAVIRUS diseases , *DIAGNOSIS , *QUALITY assurance - Abstract
Inactivated severe acute respiratory syndrome--associated coronavirus samples were used for an external quality assurance study within the World Health Organization SARS Reference and Verification Network and other reference institutions. Of 58 participants, 51 correctly detected virus in all samples ≥9,400 RNA copies per milliliter and none in negative samples. Commercial test kits significantly improved the outcome. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
46. Dengue Virus Serotype 3 Infection in Traveler Returning from West Africa to Germany.
- Author
-
Eckerle, Isabella, Kapaun, Annette, Junghanss, Thomas, Schnitzler, Paul, Drosten, Christian, and Jänisch, Thomas
- Subjects
DENGUE viruses ,TRAVEL hygiene - Abstract
The article presents a case study of a 71-year-old-man who was infected with dengue virus (DENV) serotype 3 infection after traveling to Tongo and returning to Germany from September to October 2013. The man tested positive for DENV non-structural protein 1 and IgM against DENV by sequencing a 1,479-nt fragment of the complete gene of the envelope glycoprotein gene. It is suggested that medical response is urgently needed for dengue in Africa.
- Published
- 2015
- Full Text
- View/download PDF
47. Close Relative of Human Middle East Respiratory Syndrome Coronavirus in Bat, South Africa.
- Author
-
Ithete, Ndapewa Laudika, Stoffberg, Samantha, Corman, Victor Max, Cottontail, Veronika M., Richards, Leigh Rosanne, Schoeman, M. Corrie, Drosten, Christian, Drexler, Jan Felix, and Preiser, Wolfgang
- Subjects
PIPISTRELLUS ,CORONAVIRUS diseases ,MIDDLE East respiratory syndrome ,ANIMAL droppings - Abstract
The article discusses a study which described European Pipistrellus bat-derived coronavirus (CoVs) that are closely related to Middle East respiratory syndrome (MERS)-CoV. The researchers collected fecal pellets from 62 bats representing 13 different species in the KwaZulu-Natal and Western Cape Provinces of South Africa. The relationship between PML/2011 and MERS-CoV was confirmed by a Bayesian phylogenetic analysis.
- Published
- 2013
- Full Text
- View/download PDF
48. Human Parvovirus 4 Viremia in Young Children, Ghana.
- Author
-
May, Jürgen, Drexler, Jan Felix, Reber, Ulrike, Sarpong, Nimarko, Adjei, Ohene, Panning, Marcus, Drosten, Christian, and Eis-Hübinger, Anna Maria
- Subjects
PARVOVIRUS diseases ,VIRAL diseases in children ,BLOOD plasma ,POLYMERASE chain reaction ,VIREMIA ,CASE studies - Abstract
The article describes several cases of human parvovirus 4 (PARV4) in young children with unknown parenteral blood exposure in Ashanti, Ghana. A number of plasma samples were collected over a January-December 2004 period. Real-time polymerase chain reaction was used to analyze all the samples. According to the study, 32 of 361 children tested positive for the PARV4 genotype 3 deoxyribonucleic acid (DNA). It also detected PARV4 viremia status in 78 children aged 24 months.
- Published
- 2012
- Full Text
- View/download PDF
49. Hantavirus in Bat, Sierra Leone.
- Author
-
Weiss, Sabrina, Witkowski, Peter T., Auste, Brita, Nowak, Kathrin, Weber, Natalie, Fahr, Jakob, Mombouli, Jean-Vivien, Wolfe, Nathan D., Drexler, Jan Felix, Drosten, Christian, Klempa, Boris, Leendertz, Fabian H., and Kruger, Detlev H.
- Subjects
HANTAVIRUSES ,BATS as carriers of disease ,INFECTIOUS disease transmission ,EMERGING infectious diseases ,RESEARCH - Abstract
This article presents a study which investigated hantaviruses in bats from Sierra Leone. Hantaviruses cause life-threatening human diseases such as hantavirus cardiopulmonary syndrome and hemorrhagic fever. These viruses are transmitted from rodent reservoirs to humans. For the study, researchers examined 525 tissue samples from 417 bats representing 28 genera for the presence of hantavirus RNA. One sample yielded a positive result and it was identified belonging to a Nycteris hispida species. The researchers concluded that further research is necessary to verify Nycteris hispida as a natural reservoir host.
- Published
- 2012
- Full Text
- View/download PDF
50. Human Cardioviruses, Meningitis, and Sudden Infant Death Syndrome in Children.
- Author
-
Felix Drexler, Jan, Baumgarte, Sigrid, Eschbach-Bludau, Monika, Simon, Arne, Kemen, Christoph, Bode, Udo, Eis-Hübinger, Anna-Maria, Madea, Burkhard, and Drosten, Christian
- Subjects
MENINGITIS ,SUDDEN infant death syndrome ,CEREBROSPINAL fluid ,GENETICS - Abstract
Cardioviruses cause myocarditis and encephalomyelitis in rodents; human cardioviruses have not been ascribed to any disease. We screened 6,854 cerebrospinal fluid and 10 myocardium specimens from children and adults. A genotype 2 cardiovirus was detected from a child who died of sudden infant death syndrome, and 2 untypeable cardioviruses were detected from 2 children with meningitis. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
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