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Research has been conducted on the maltose-producing amylase inhibitor. The preparation of this inhibitor carried out via the high-molecular-weight acarbose degradation is described.

Research has been conducted on the ketosteroid isomerase from Pseudomonas putida biotype B. The structural and functional roles of the tyrosine residues' phenyl rings in the active site of P. putida ketosteroid isomerase have been investigated and discussed.

Research has been conducted on the thermostable phytase from Bacillus amyloliquifaciens DS11. The Ca (super)2+ -dependent catalytic properties of the phytase and its specific substrate requirement have been investigated.

Research has been conducted on the delta (super)5 -3-ketosteroid isomerase (KSI) from Pseudomonas putida biotype B. The crystal structures of Y 14F and the structural basis for the pseudoreversion in the KSI have been investigated.

Results demonstrate that survivin mediates its anti-apoototic activity by sequestering caspase-3 and -7 in their inhibited states. Data indicate that the protein binds to caspase-3 and -7 and inhibits the cleavage of the physiological substrates of the enzymes during the G2/M phase of the cell cycle.

Results reveal that catalysis of allylic isomerization by ketosteroid isomerase involves, among others, the catalytic residues charging the buffer to stabilize the negative charge of the intermediates. Data indicate that the hydrogen bond distance of the intermediate analogues is about 0.2 angstrom shorter in the product analogues.

Research has been conducted on glutamate 332. The role of Glu332 in the hydrolysis and transglycosylation activity of Thermus maltogenic amylases has been investigated via site-directed mutagenesis.

Research has been conducted on the Pseudomonas putida biotype B ketosteroid isomerase. The catalysis of the allylic isomerisation reaction is discussed.

Results indicate that amino acid aspartate 99 donates a hydrogen bond to the steroid intermediate directly in the catalytic reaction of isomerase from Pseudomonas putida Biotype B.

Analyzes the reactivity of high resolution Delta 5-3-ketosteroid isomerase crystal structures with a catalytic intermediate analogue. Protein purification and crystallization; Multiple isomorphous replacement method; Protein fold and structural features; Active-site residues; Kinetic analysis of mutant reduction.