Pseudoreversion of the catalytic activity of Y 14F by the additional substitution(s) of tyrosine with phenylalanine in the hydrogen bond network of delta (super)5 -3-ketosteroid isomerase from Pseudomonas putida biotype

Authors :: Gildon Choi
Nam-Chul Ha
Min-Sung Kim
Bee-Hak Hong
Byung-Ha Oh
Kwan Yong Choi
Source :: Biochemistry. June 12, 2001, Vol. 40 Issue 23, p6828, 8 p.
Publication Year :: 2001
Abstract: Research has been conducted on the delta (super)5 -3-ketosteroid isomerase (KSI) from Pseudomonas putida biotype B. The crystal structures of Y 14F and the structural basis for the pseudoreversion in the KSI have been investigated.

Subjects :: Biochemistry -- Research
Tyrosine -- Research
Phenylalanine -- Research
Hydrogen -- Research
Isomerases -- Research
Pseudomonas -- Research
Biological sciences
Chemistry

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