1. Paper Chromatography of Glucoside of Rice Bran
- Author
-
Hisaya Horiuchi and Yoshijiro Kihara
- Subjects
Chromatography ,Spots ,Bran ,Medicine (miscellaneous) ,chemistry.chemical_element ,Fraction (chemistry) ,Zinc ,Paper chromatography ,chemistry.chemical_compound ,chemistry ,Glucoside ,Chemistry (miscellaneous) ,Selective adsorption ,Reagent ,Food Science ,Biotechnology - Abstract
A new glucoside, Nukain, was isolated from the water extract of rice bran as previously reported in this Journal 19, 577 (1943). The properties of Nukain and Nukagenin were investigated by the paper chromatography. The fluorescent spots on paper were observed under ultra-violet ray lamp. Though Nukain showed a tailing figure, Nukagenin gave a single spot. An yellow coloring matter isolated from the fraction precipitated with basic lead acetate showed 3 spots. The paper chromatography of the sugar fraction of Nukain by anilinehydro-genphthalate gave the figure of glucose. The selective adsorption of Nukain by ion exchange resin could not be remarkedly observed. An yellow coloring matter could not be adsorbed by both anion-or cation-exchange resin. Nukain and Nukagenin gave the characteristic spots by paper electrophoresis as snown in Table 5 and 6. When Nukain was treated with Takadiasta. se and emulsin two spots were observed as shown in Table 7 but the spot of Nukagenin could not be found. The zinc dust distillation product of Nukagenin showed a spot on paper chro-matogram by Dragendorf's reagent, which was identical with that of quinolin. Nukagenin may have a quinolin nucleus.
- Published
- 1954