The Developing Solvent and Spraying Reagent to the Paper Chromatogrphy of Phenols

As the developing solvent in the paper chromatography of phenols, we found that the mix-ture of n-butanol, benzene, acetic acid, and water (1:5:1:0.5 by vol. %) is better than other solvents so for reported, (Table 1) and p-benzoquinone and p-nitrosodimethylaniline are preferable as the spraying reagent (Table 2).